Q96CJ1 (EAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ELL-associated factor 2

Alternative name(s):
Testosterone-regulated apoptosis inducer and tumor suppressor protein

Gene names

Name:	EAF2
Synonyms:	TRAITS
ORF Names:	BM-040

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional transactivator of TCEA1 elongation activity By similarity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Ref.1 Ref.2 Ref.5
Subunit structure	Isoform 1 and isoform 2 interact with TCEA1 By similarity. Interacts with ELL and ELL2. Ref.1
Subcellular location	Nucleus speckle. Note: Colocalizes with ELL to nuclear speckles. Ref.1 Ref.2
Tissue specificity	Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes. Ref.1 Ref.2
Induction	By androgen. Ref.2
Sequence similarities	Belongs to the EAF family.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TFPT	P0C1Z6	4	EBI-1245604,EBI-1245626

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96CJ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96CJ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	ELL-associated factor 2		PRO_0000130337
Regions
	Region	17 – 104	88	Necessary for interaction with ELL
	Region	177 – 260	84	Necessary for transactivation activity
	Region	246 – 260	15	Necessary for interaction with TCEA1 and transactivation activity By similarity
	Compositional bias	126 – 130	5	Poly-Gln
	Compositional bias	174 – 207	34	Ser-rich
Amino acid modifications
	Modified residue	146	1	Phosphoserine Ref.6
	Modified residue	151	1	Phosphoserine Ref.6
	Modified residue	154	1	Phosphoserine Ref.6 Ref.7
Natural variations
	Alternative sequence	1 – 130	130	Missing in isoform 2.		VSP_015310

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2CDCE96FA387C799	FASTA	260	28,792
	10 20 30 40 50 60 MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT 70 80 90 100 110 120 ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI 130 140 150 160 170 180 QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS 190 200 210 220 230 240 DSKSSSSSSS EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL 250 260 LMNTLRNDLQ LSESGSDSDD
	Isoform 2 [UniParc]. Checksum: AEDE38BF25A5014B	FASTA	130	14,091
	10 20 30 40 50 60 MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS 70 80 90 100 110 120 EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ 130 LSESGSDSDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties." Simone F., Luo R.T., Polak P.E., Kaberlein J.J., Thirman M.J. Blood 101:2355-2362(2003) [PubMed: 12446457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN TRANSCRIPTION ACTIVATION, INTERACTION WITH ELL AND ELL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Bone marrow.
[2]	"Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer." Xiao W., Zhang Q., Jiang F., Pins M., Kozlowski J.M., Wang Z. Cancer Res. 63:4698-4704(2003) [PubMed: 12907652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN APOPTOSIS, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Bone marrow.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: B-cell.
[5]	"ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL." Kong S.E., Banks C.A., Shilatifard A., Conaway J.W., Conaway R.C. Proc. Natl. Acad. Sci. U.S.A. 102:10094-10098(2005) [PubMed: 16006523] [Abstract] Cited for: ROLE IN ELL-TRANSCRIPTION ACTIVATION.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-151 AND SER-154, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF517829 mRNA. Translation: AAO63811.1. AY049020 mRNA. Translation: AAL12223.1. AF217516 mRNA. Translation: AAF67627.1. BC014209 mRNA. Translation: AAH14209.1.
IPI	IPI00642124. IPI00942345.
RefSeq	NP_060926.2. NM_018456.4.
UniGene	Hs.477325.
3D structure databases
ProteinModelPortal	Q96CJ1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96CJ1. 5 interactions.
STRING	Q96CJ1.
PTM databases
PhosphoSite	Q96CJ1.
Polymorphism databases
DMDM	73919269.
Proteomic databases
PRIDE	Q96CJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000273668; ENSP00000273668; ENSG00000145088.
GeneID	55840.
KEGG	hsa:55840.
UCSC	uc003een.1. human. uc003eeo.1. human.
Organism-specific databases
CTD	55840.
GeneCards	GC03P121555.
H-InvDB	HIX0003596.
HGNC	HGNC:23115. EAF2.
HPA	HPA008411.
MIM	607659. gene.
neXtProt	NX_Q96CJ1.
PharmGKB	PA128394690.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06484.
GeneTree	ENSGT00390000017724.
HOVERGEN	HBG054898.
InParanoid	Q96CJ1.
OrthoDB	EOG4W6NX6.
PhylomeDB	Q96CJ1.
Gene expression databases
ArrayExpress	Q96CJ1.
Bgee	Q96CJ1.
CleanEx	HS_EAF2.
Genevestigator	Q96CJ1.
GermOnline	ENSG00000145088. Homo sapiens.
Family and domain databases
InterPro	IPR019194. Tscrpt_elong_fac_Eaf_N. [Graphical view]
KO	K15186.
Pfam	PF09816. EAF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	61073.
SOURCE	Search...

Entry information

Entry name

EAF2_HUMAN

Accession

Primary (citable) accession number: Q96CJ1
Secondary accession number(s): Q9NZ82

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.