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Q96CJ1 (EAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ELL-associated factor 2
Alternative name(s):
Testosterone-regulated apoptosis inducer and tumor suppressor protein
Gene names
Name:EAF2
Synonyms:TRAITS
ORF Names:BM-040
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional transactivator of TCEA1 elongation activity By similarity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Ref.1 Ref.2 Ref.5

Subunit structure

Isoform 1 and isoform 2 interact with TCEA1 By similarity. Interacts with ELL and ELL2. Ref.1

Subcellular location

Nucleus speckle. Note: Colocalizes with ELL to nuclear speckles. Ref.1 Ref.2

Tissue specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes. Ref.1 Ref.2

Induction

By androgen. Ref.2

Sequence similarities

Belongs to the EAF family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TFPTP0C1Z64EBI-1245604,EBI-1245626

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96CJ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96CJ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260ELL-associated factor 2
PRO_0000130337

Regions

Region17 – 10488Necessary for interaction with ELL
Region177 – 26084Necessary for transactivation activity
Region246 – 26015Necessary for interaction with TCEA1 and transactivation activity By similarity
Compositional bias126 – 1305Poly-Gln
Compositional bias174 – 20734Ser-rich

Amino acid modifications

Modified residue1511Phosphoserine Ref.6
Modified residue1541Phosphoserine Ref.6 Ref.7

Natural variations

Alternative sequence1 – 130130Missing in isoform 2.
VSP_015310

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 2CDCE96FA387C799

FASTA26028,792
        10         20         30         40         50         60 
MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT 

        70         80         90        100        110        120 
ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI 

       130        140        150        160        170        180 
QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS 

       190        200        210        220        230        240 
DSKSSSSSSS EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL 

       250        260 
LMNTLRNDLQ LSESGSDSDD 

« Hide

Isoform 2 [UniParc].

Checksum: AEDE38BF25A5014B
Show »

FASTA13014,091

References

« Hide 'large scale' references
[1]"ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties."
Simone F., Luo R.T., Polak P.E., Kaberlein J.J., Thirman M.J.
Blood 101:2355-2362(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH ELL AND ELL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Bone marrow.
[2]"Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer."
Xiao W., Zhang Q., Jiang F., Pins M., Kozlowski J.M., Wang Z.
Cancer Res. 63:4698-4704(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN APOPTOSIS, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Bone marrow.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: B-cell.
[5]"ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL."
Kong S.E., Banks C.A., Shilatifard A., Conaway J.W., Conaway R.C.
Proc. Natl. Acad. Sci. U.S.A. 102:10094-10098(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF517829 mRNA. Translation: AAO63811.1.
AY049020 mRNA. Translation: AAL12223.1.
AF217516 mRNA. Translation: AAF67627.1.
BC014209 mRNA. Translation: AAH14209.1.
CCDSCCDS3006.1. [Q96CJ1-1]
RefSeqNP_060926.2. NM_018456.4. [Q96CJ1-1]
XP_005247677.1. XM_005247620.1. [Q96CJ1-2]
UniGeneHs.477325.

3D structure databases

ProteinModelPortalQ96CJ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120943. 5 interactions.
IntActQ96CJ1. 7 interactions.
STRING9606.ENSP00000273668.

PTM databases

PhosphoSiteQ96CJ1.

Polymorphism databases

DMDM73919269.

Proteomic databases

MaxQBQ96CJ1.
PaxDbQ96CJ1.
PRIDEQ96CJ1.

Protocols and materials databases

DNASU55840.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273668; ENSP00000273668; ENSG00000145088. [Q96CJ1-1]
GeneID55840.
KEGGhsa:55840.
UCSCuc003een.3. human. [Q96CJ1-1]

Organism-specific databases

CTD55840.
GeneCardsGC03P121555.
HGNCHGNC:23115. EAF2.
HPAHPA008411.
MIM607659. gene.
neXtProtNX_Q96CJ1.
PharmGKBPA128394690.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG273677.
HOGENOMHOG000070137.
HOVERGENHBG054898.
InParanoidQ96CJ1.
KOK15186.
PhylomeDBQ96CJ1.
TreeFamTF320864.

Gene expression databases

ArrayExpressQ96CJ1.
BgeeQ96CJ1.
CleanExHS_EAF2.
GenevestigatorQ96CJ1.

Family and domain databases

InterProIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERPTHR15970. PTHR15970. 1 hit.
PfamPF09816. EAF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiEAF2.
GenomeRNAi55840.
NextBio61073.
PROQ96CJ1.
SOURCESearch...

Entry information

Entry nameEAF2_HUMAN
AccessionPrimary (citable) accession number: Q96CJ1
Secondary accession number(s): Q9NZ82
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM