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Protein

ELL-associated factor 2

Gene

EAF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.By similarity3 Publications

GO - Molecular functioni

  1. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. negative regulation of cell growth Source: Ensembl
  3. negative regulation of epithelial cell proliferation involved in prostate gland development Source: Ensembl
  4. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
ELL-associated factor 2
Alternative name(s):
Testosterone-regulated apoptosis inducer and tumor suppressor protein
Gene namesi
Name:EAF2
Synonyms:TRAITS
ORF Names:BM-040
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:23115. EAF2.

Subcellular locationi

Nucleus speckle 2 Publications
Note: Colocalizes with ELL to nuclear speckles.

GO - Cellular componenti

  1. ELL-EAF complex Source: InterPro
  2. nuclear speck Source: UniProtKB-SubCell
  3. transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394690.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260ELL-associated factor 2PRO_0000130337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511Phosphoserine1 Publication
Modified residuei154 – 1541Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96CJ1.
PaxDbiQ96CJ1.
PRIDEiQ96CJ1.

PTM databases

PhosphoSiteiQ96CJ1.

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.2 Publications

Inductioni

By androgen.1 Publication

Gene expression databases

BgeeiQ96CJ1.
CleanExiHS_EAF2.
ExpressionAtlasiQ96CJ1. baseline and differential.
GenevestigatoriQ96CJ1.

Organism-specific databases

HPAiHPA008411.

Interactioni

Subunit structurei

Isoform 1 and isoform 2 interact with TCEA1 (By similarity). Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL and ELL2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TFPTP0C1Z64EBI-1245604,EBI-1245626

Protein-protein interaction databases

BioGridi120943. 10 interactions.
IntActiQ96CJ1. 7 interactions.
STRINGi9606.ENSP00000273668.

Structurei

3D structure databases

ProteinModelPortaliQ96CJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 10488Necessary for interaction with ELLAdd
BLAST
Regioni177 – 26084Necessary for transactivation activityAdd
BLAST
Regioni246 – 26015Necessary for interaction with TCEA1 and transactivation activityBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi126 – 1305Poly-Gln
Compositional biasi174 – 20734Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the EAF family.Curated

Phylogenomic databases

eggNOGiNOG273677.
GeneTreeiENSGT00390000017724.
HOGENOMiHOG000070137.
HOVERGENiHBG054898.
InParanoidiQ96CJ1.
KOiK15186.
PhylomeDBiQ96CJ1.
TreeFamiTF320864.

Family and domain databases

InterProiIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERiPTHR15970. PTHR15970. 1 hit.
PfamiPF09816. EAF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96CJ1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY
60 70 80 90 100
LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL
110 120 130 140 150
EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM
160 170 180 190 200
SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDE
210 220 230 240 250
DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ
260
LSESGSDSDD
Length:260
Mass (Da):28,792
Last modified:December 1, 2001 - v1
Checksum:i2CDCE96FA387C799
GO
Isoform 2 (identifier: Q96CJ1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Show »
Length:130
Mass (Da):14,091
Checksum:iAEDE38BF25A5014B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 130130Missing in isoform 2. 1 PublicationVSP_015310Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF517829 mRNA. Translation: AAO63811.1.
AY049020 mRNA. Translation: AAL12223.1.
AF217516 mRNA. Translation: AAF67627.1.
BC014209 mRNA. Translation: AAH14209.1.
CCDSiCCDS3006.1. [Q96CJ1-1]
RefSeqiNP_060926.2. NM_018456.4. [Q96CJ1-1]
XP_005247677.1. XM_005247620.1. [Q96CJ1-2]
UniGeneiHs.477325.

Genome annotation databases

EnsembliENST00000273668; ENSP00000273668; ENSG00000145088. [Q96CJ1-1]
GeneIDi55840.
KEGGihsa:55840.
UCSCiuc003een.3. human. [Q96CJ1-1]

Polymorphism databases

DMDMi73919269.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF517829 mRNA. Translation: AAO63811.1.
AY049020 mRNA. Translation: AAL12223.1.
AF217516 mRNA. Translation: AAF67627.1.
BC014209 mRNA. Translation: AAH14209.1.
CCDSiCCDS3006.1. [Q96CJ1-1]
RefSeqiNP_060926.2. NM_018456.4. [Q96CJ1-1]
XP_005247677.1. XM_005247620.1. [Q96CJ1-2]
UniGeneiHs.477325.

3D structure databases

ProteinModelPortaliQ96CJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120943. 10 interactions.
IntActiQ96CJ1. 7 interactions.
STRINGi9606.ENSP00000273668.

PTM databases

PhosphoSiteiQ96CJ1.

Polymorphism databases

DMDMi73919269.

Proteomic databases

MaxQBiQ96CJ1.
PaxDbiQ96CJ1.
PRIDEiQ96CJ1.

Protocols and materials databases

DNASUi55840.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273668; ENSP00000273668; ENSG00000145088. [Q96CJ1-1]
GeneIDi55840.
KEGGihsa:55840.
UCSCiuc003een.3. human. [Q96CJ1-1]

Organism-specific databases

CTDi55840.
GeneCardsiGC03P121555.
HGNCiHGNC:23115. EAF2.
HPAiHPA008411.
MIMi607659. gene.
neXtProtiNX_Q96CJ1.
PharmGKBiPA128394690.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG273677.
GeneTreeiENSGT00390000017724.
HOGENOMiHOG000070137.
HOVERGENiHBG054898.
InParanoidiQ96CJ1.
KOiK15186.
PhylomeDBiQ96CJ1.
TreeFamiTF320864.

Miscellaneous databases

GeneWikiiEAF2.
GenomeRNAii55840.
NextBioi61073.
PROiQ96CJ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96CJ1.
CleanExiHS_EAF2.
ExpressionAtlasiQ96CJ1. baseline and differential.
GenevestigatoriQ96CJ1.

Family and domain databases

InterProiIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERiPTHR15970. PTHR15970. 1 hit.
PfamiPF09816. EAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties."
    Simone F., Luo R.T., Polak P.E., Kaberlein J.J., Thirman M.J.
    Blood 101:2355-2362(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH ELL AND ELL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Bone marrow.
  2. "Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer."
    Xiao W., Zhang Q., Jiang F., Pins M., Kozlowski J.M., Wang Z.
    Cancer Res. 63:4698-4704(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN APOPTOSIS, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Bone marrow.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: B-cell.
  5. "ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL."
    Kong S.E., Banks C.A., Shilatifard A., Conaway J.W., Conaway R.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:10094-10098(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. Cited for: IDENTIFICATION IN THE SEC COMPLEX.

Entry informationi

Entry nameiEAF2_HUMAN
AccessioniPrimary (citable) accession number: Q96CJ1
Secondary accession number(s): Q9NZ82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: February 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.