Q96CD2 (COAC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphopantothenoylcysteine decarboxylase
Short name=PPC-DC
EC=4.1.1.36

Alternative name(s):
CoaC

Gene names

Name:	PPCDC
Synonyms:	COAC
ORF Names:	MDS018, UNQ9365/PRO34154

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the biosynthesis of coenzyme A. Catalyzes the decarboxylation of 4-phosphopantothenoylcysteine to form 4'-phosphopantotheine. Ref.6 Ref.7
Catalytic activity	N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.
Cofactor	Binds 1 FMN per subunit.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.
Subunit structure	Homotrimer Potential.
Sequence similarities	Belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	FMN Flavoprotein
Molecular function	Decarboxylase Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pantothenate metabolic process Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	phosphopantothenoylcysteine decarboxylase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96CD2-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96CD2-2) The sequence of this isoform differs from the canonical sequence as follows:      1-77: Missing.      78-78: I → M
Note: Produced only in the presence of the Met-1 variant.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Natural variant	1	1	M → I. Corresponds to variant rs2304899 [ dbSNP | Ensembl ].

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

Phosphopantothenoylcysteine decarboxylase

PRO_0000182030

Regions

Nucleotide binding

104 – 107

4

FMN

Sites

Active site

173

1

Proton donor

Binding site

59

1

FMN

Binding site

140

1

Substrate By similarity

Natural variations

Alternative sequence

1 – 77

77

Missing in isoform 2.

VSP_014003

Alternative sequence

78

1

I → M in isoform 2.

VSP_039201

Experimental info

Sequence conflict

49

1

A → S in BAB55151. Ref.3

Secondary structure

1

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204

Helix Strand Turn

Details...

Beta strand

16 – 24

9

Helix

28 – 31

4

Helix

33 – 40

8

Beta strand

46 – 52

7

Helix

55 – 58

4

Helix

62 – 64

3

Helix

72 – 77

6

Helix

87 – 92

6

Beta strand

96 – 103

8

Helix

105 – 112

8

Helix

119 – 125

7

Beta strand

135 – 137

3

Helix

141 – 144

4

Helix

149 – 157

9

Helix

186 – 191

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 9E59683480032894	FASTA	204	22,395
	10 20 30 40 50 60 MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY 70 80 90 100 110 120 SPQDIPVTLY SDADEWEIWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL 130 140 150 160 170 180 TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA 190 200 MAEVGTIVDK VKEVLFQHSG FQQS
	Isoform 2 [UniParc]. Checksum: 562D659DE77C0AF5	FASTA	127	14,069
	10 20 30 40 50 60 MWKSRSDPVL HIDLRRWADL LLVAPLDANT LGKVASGICD NLLTCVMRAW DRSKPLLFCP 70 80 90 100 110 120 AMNTAMWEHP ITAQQVDQLK AFGYVEIPCV AKKLVCGDEG LGAMAEVGTI VDKVKEVLFQ HSGFQQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Huang C., Qian B., Tu Y., Gu W., Wang Y., Han Z., Chen Z. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hematopoietic stem cell.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[6]	"Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics." Daugherty M., Polanuyer B., Farrell M., Scholle M., Lykidis A., de Crecy-Lagard V., Osterman A. J. Biol. Chem. 277:21431-21439(2002) [PubMed: 11923312] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[7]	"Mechanistic studies on phosphopantothenoylcysteine decarboxylase: trapping of an enethiolate intermediate with a mechanism-based inactivating agent." Strauss E., Zhai H., Brand L.A., McLafferty F.W., Begley T.P. Biochemistry 43:15520-15533(2004) [PubMed: 15581364] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY.
[8]	"Unusual space-group pseudosymmetry in crystals of human phosphopantothenoylcysteine decarboxylase." Manoj N., Ealick S.E. Acta Crystallogr. D 59:1762-1766(2003) [PubMed: 14501115] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.91 ANGSTROMS) IN COMPLEX WITH FMN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF182419 mRNA. Translation: AAG14955.1.
AY358848 mRNA. Translation: AAQ89207.1.
AK027491 mRNA. Translation: BAB55151.1.
AC015720 Genomic DNA. No translation available.
BC014409 mRNA. Translation: AAH14409.1.

IPI

IPI00303962.
IPI00604702.

RefSeq

NP_068595.3. NM_021823.3.

UniGene

Hs.458922.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QZU	X-ray	2.91	A/B/C/D	1-204	[»]

ProteinModelPortal

Q96CD2.

SMR

Q96CD2. Positions 14-194.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96CD2. 3 interactions.

MINT

MINT-1394867.

STRING

Q96CD2.

Polymorphism databases

DMDM

296434457.

Proteomic databases

PRIDE

Q96CD2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000342932; ENSP00000343190; ENSG00000138621.

GeneID

60490.

KEGG

hsa:60490.

UCSC

uc002azo.1. human.

Organism-specific databases

CTD

60490.

GeneCards

GC15P075315.

H-InvDB

HIX0202140.

HGNC

HGNC:28107. PPCDC.

MIM

609854. gene.

neXtProt

NX_Q96CD2.

PharmGKB

PA142671157.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10187.

GeneTree

ENSGT00440000038107.

HOGENOM

HBG523890.

HOVERGEN

HBG054722.

InParanoid

Q96CD2.

OMA

APLMERK.

OrthoDB

EOG42NJ1C.

PhylomeDB

Q96CD2.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

Q96CD2.

Bgee

Q96CD2.

CleanEx

HS_PPCDC.

Genevestigator

Q96CD2.

GermOnline

ENSG00000138621. Homo sapiens.

Family and domain databases

InterPro

IPR003382. Flavoprotein.
[Graphical view]

Gene3D

G3DSA:3.40.50.1950. Flavoprotein. 1 hit.

KO

K01598.

Pfam

PF02441. Flavoprotein. 1 hit.
[Graphical view]

SUPFAM

SSF52507. Flavoprotein. 1 hit.

ProtoNet

Search...

Other

NextBio

65379.

SOURCE

Search...

Entry information

Entry name

COAC_HUMAN

Accession

Primary (citable) accession number: Q96CD2
Secondary accession number(s): Q96SX0, Q9HC17

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.