Q96CB8 (INT12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrator complex subunit 12
Short name=Int12

Alternative name(s):
PHD finger protein 22

Gene names

Name:	INTS12
Synonyms:	PHF22
ORF Names:	SBBI22

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Subunit structure	Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Ref.5
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 PHD-type zinc finger.
Sequence caution	The sequence AAF99604.1 differs from that shown. Reason: Frameshift at position 439.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	snRNA processing Inferred from direct assay Ref.5. Source: HGNC
Cellular_component	integrator complex Inferred from direct assay Ref.5. Source: HGNC
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Integrator complex subunit 12		PRO_0000059312
Regions
	Zinc finger	159 – 215	57	PHD-type
	Compositional bias	270 – 440	171	Ser-rich
Amino acid modifications
	Modified residue	128	1	Phosphoserine Ref.8
Natural variations
	Natural variant	323	1	T → A. Corresponds to variant rs34567094 [ dbSNP | Ensembl ].		VAR_049629

Sequences

	Sequence		Length	Mass (Da)
	Q96CB8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7AEF952044A3E951	FASTA	462	48,808
	10 20 30 40 50 60 MAATVNLELD PIFLKALGFL HSKSKDSAEK LKALLDESLA RGIDSSYRPS QKDVEPPKIS 70 80 90 100 110 120 STKNISIKQE PKISSSLPSG NNNGKVLTTE KVKKEAEKRP ADKMKSDITE GVDIPKKPRL 130 140 150 160 170 180 EKPETQSSPI TVQSSKDLPM ADLSSFEETS ADDFAMEMGL ACVVCRQMMV ASGNQLVECQ 190 200 210 220 230 240 ECHNLYHRDC HKPQVTDKEA NDPRLVWYCA RCTRQMKRMA QKTQKPPQKP APAVVSVTPA 250 260 270 280 290 300 VKDPLVKKPE TKLKQETTFL AFKRTEVKTS TVISGNSSSA SVSSSVTSGL TGWAAFAAKT 310 320 330 340 350 360 SSAGPSTAKL SSTTQNNTGK PATSSANQKP VGLTGLATSS KGGIGSKIGS NNSTTPTVPL 370 380 390 400 410 420 KPPPPLTLGK TGLSRSVSCD NVSKVGLPSP SSLVPGSSSQ LSGNGNSGTS GPSGSTTSKT 430 440 450 460 TSESSSSPSA SLKGPTSQES QLNAMKRLQM VKKKAAQKKL KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hypothetical nuclear factor SBBI22 mRNA." Wan T., Li N., Zhang W., Cao X. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[5]	"Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II." Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N., Shiekhattar R. Cell 123:265-276(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR COMPLEX.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF242524 mRNA. Translation: AAF99604.1. Frameshift. AK314939 mRNA. Translation: BAG37445.1. CH471057 Genomic DNA. Translation: EAX06189.1. BC014442 mRNA. Translation: AAH14442.1. BK005729 mRNA. Translation: DAA05729.1.
IPI	IPI00060379.
RefSeq	NP_001135943.1. NM_001142471.1. NP_065128.2. NM_020395.3.
UniGene	Hs.480454.
3D structure databases
ProteinModelPortal	Q96CB8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48479N.
STRING	9606.ENSP00000265150.
PTM databases
PhosphoSite	Q96CB8.
Polymorphism databases
DMDM	73621394.
Proteomic databases
PaxDb	Q96CB8.
PeptideAtlas	Q96CB8.
PRIDE	Q96CB8.
Protocols and materials databases
DNASU	57117.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000340139; ENSP00000340737; ENSG00000138785. ENST00000394735; ENSP00000378221; ENSG00000138785. ENST00000451321; ENSP00000415433; ENSG00000138785.
GeneID	57117.
KEGG	hsa:57117.
UCSC	uc003hxw.3. human.
Organism-specific databases
CTD	57117.
GeneCards	GC04M106603.
HGNC	HGNC:25067. INTS12.
HPA	HPA035772.
MIM	611355. gene.
neXtProt	NX_Q96CB8.
PharmGKB	PA142671177.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG247951.
HOGENOM	HOG000059634.
HOVERGEN	HBG081798.
InParanoid	Q96CB8.
KO	K13149.
OMA	KPRLEKQ.
OrthoDB	EOG4Q58Q7.
PhylomeDB	Q96CB8.
Gene expression databases
ArrayExpress	Q96CB8.
Bgee	Q96CB8.
CleanEx	HS_INTS12.
Genevestigator	Q96CB8.
GermOnline	ENSG00000138785. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00628. PHD. 1 hit. [Graphical view]
SMART	SM00249. PHD. 1 hit. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57117.
NextBio	62983.
SOURCE	Search...

Entry information

Entry name

INT12_HUMAN

Accession

Primary (citable) accession number: Q96CB8
Secondary accession number(s): B2RC48, Q3B6Z3, Q9HD71

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.