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Protein

Baculoviral IAP repeat-containing protein 7

Gene

BIRC7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.4 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi124Zinc1
Metal bindingi127Zinc1
Metal bindingi144Zinc1
Metal bindingi151Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri252 – 286RING-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • activation of JUN kinase activity Source: UniProtKB
  • apoptotic process Source: UniProtKB-KW
  • inhibition of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
  • mitotic spindle assembly Source: GO_Central
  • negative regulation of apoptotic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of cell proliferation Source: UniProtKB
  • regulation of natural killer cell apoptotic process Source: UniProtKB
  • regulation of signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor, Transferase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101197-MONOMER.
SignaLinkiQ96CA5.
SIGNORiQ96CA5.

Protein family/group databases

MEROPSiI32.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Baculoviral IAP repeat-containing protein 7 (EC:2.3.2.271 Publication)
Alternative name(s):
Kidney inhibitor of apoptosis protein
Short name:
KIAP
Livin
Melanoma inhibitor of apoptosis protein
Short name:
ML-IAP
RING finger protein 50
RING-type E3 ubiquitin transferase BIRC7Curated
Cleaved into the following chain:
Baculoviral IAP repeat-containing protein 7 30kDa subunit
Short name:
Truncated livin
Short name:
p30-Livin
Short name:
tLivin
Gene namesi
Name:BIRC7
Synonyms:KIAP, LIVIN, MLIAP, RNF50
ORF Names:UNQ5800/PRO19607/PRO21344
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:13702. BIRC7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • nucleus Source: UniProtKB
  • spindle microtubule Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87 – 88EE → AA: No change in SMAC interaction and anti-apoptotic activity. 1 Publication2
Mutagenesisi120D → A: Abolishes inhibition of caspases, SMAC binding and anti-apoptotic activity. 1 Publication1
Mutagenesisi124C → A: Abolishes inhibition of caspases and anti-apoptotic activity. 1 Publication1
Mutagenesisi138D → A: Abolishes inhibition of caspases, SMAC binding and anti-apoptotic activity. 1 Publication1

Organism-specific databases

DisGeNETi79444.
OpenTargetsiENSG00000101197.
PharmGKBiPA25364.

Chemistry databases

ChEMBLiCHEMBL1075127.
GuidetoPHARMACOLOGYi2794.

Polymorphism and mutation databases

BioMutaiBIRC7.
DMDMi21759008.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223621 – 298Baculoviral IAP repeat-containing protein 7Add BLAST298
ChainiPRO_000041609253 – 298Baculoviral IAP repeat-containing protein 7 30kDa subunitAdd BLAST246

Post-translational modificationi

Autoubiquitinated and undergoes proteasome-mediated degradation.
The truncated protein (tLivin) not only loses its anti-apoptotic effect but also acquires a pro-apoptotic effect.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei52 – 53Cleavage; by CASP3 and CASP72

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ96CA5.
PaxDbiQ96CA5.
PeptideAtlasiQ96CA5.
PRIDEiQ96CA5.

PTM databases

iPTMnetiQ96CA5.
PhosphoSitePlusiQ96CA5.

Miscellaneous databases

PMAP-CutDBQ96CA5.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.

Gene expression databases

BgeeiENSG00000101197.
CleanExiHS_BIRC7.
ExpressionAtlasiQ96CA5. baseline and differential.
GenevisibleiQ96CA5. HS.

Organism-specific databases

HPAiCAB037025.
CAB037119.
HPA047850.

Interactioni

Subunit structurei

Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AESQ081173EBI-517623,EBI-717810
BIRC2Q134903EBI-517623,EBI-514538
CASP9P5521110EBI-517623,EBI-516799
DDX6P261965EBI-517623,EBI-351257
DIABLOQ9NR286EBI-517623,EBI-517508
FAM124BQ9H5Z63EBI-517623,EBI-741626
HTRA2O434642EBI-517623,EBI-517086
PHF1O431893EBI-517623,EBI-530034
POLR1CO151605EBI-517623,EBI-1055079

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122670. 32 interactors.
DIPiDIP-33486N.
IntActiQ96CA5. 27 interactors.
MINTiMINT-147031.
STRINGi9606.ENSP00000217169.

Chemistry databases

BindingDBiQ96CA5.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi82 – 84Combined sources3
Helixi87 – 92Combined sources6
Turni93 – 96Combined sources4
Turni100 – 102Combined sources3
Helixi105 – 110Combined sources6
Beta strandi113 – 115Combined sources3
Beta strandi117 – 120Combined sources4
Beta strandi122 – 124Combined sources3
Turni125 – 127Combined sources3
Beta strandi130 – 132Combined sources3
Helixi140 – 147Combined sources8
Helixi152 – 158Combined sources7
Helixi160 – 166Combined sources7
Helixi168 – 171Combined sources4
Helixi243 – 249Combined sources7
Turni253 – 255Combined sources3
Beta strandi256 – 259Combined sources4
Beta strandi262 – 265Combined sources4
Turni273 – 275Combined sources3
Helixi276 – 278Combined sources3
Turni283 – 285Combined sources3
Beta strandi291 – 294Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OXNX-ray2.20A/B/C/D/E63-179[»]
1OXQX-ray2.30A/B/C/D/E63-179[»]
1OY7X-ray2.70A/B/C/D/E63-179[»]
1TW6X-ray1.71A/B63-172[»]
2I3HX-ray1.62A/B63-172[»]
2I3IX-ray2.30A/B63-172[»]
3F7GX-ray2.30A/B/C/D/E63-179[»]
3F7HX-ray1.80A/B63-172[»]
3F7IX-ray1.90A/B63-172[»]
3GT9X-ray1.70A/B63-172[»]
3GTAX-ray1.70A/B63-172[»]
3UW5X-ray1.71A/B63-159[»]
4AUQX-ray2.18B/E239-298[»]
ProteinModelPortaliQ96CA5.
SMRiQ96CA5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96CA5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati90 – 155BIRAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi64 – 69Poly-Glu6

Domaini

The RING domain is essential for autoubiquitination.

Sequence similaritiesi

Belongs to the IAP family.Curated
Contains 1 BIR repeat.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri252 – 286RING-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1101. Eukaryota.
ENOG410YPNM. LUCA.
GeneTreeiENSGT00500000044782.
HOGENOMiHOG000232059.
HOVERGENiHBG099136.
InParanoidiQ96CA5.
KOiK16061.
OMAiFPSCQFL.
OrthoDBiEOG091G0CXH.
PhylomeDBiQ96CA5.
TreeFamiTF105356.

Family and domain databases

CDDicd00022. BIR. 1 hit.
Gene3Di1.10.1170.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001370. BIR_rpt.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00653. BIR. 1 hit.
[Graphical view]
SMARTiSM00238. BIR. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS01282. BIR_REPEAT_1. 1 hit.
PS50143. BIR_REPEAT_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q96CA5-1) [UniParc]FASTAAdd to basket
Also known as: Livin alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH
60 70 80 90 100
VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT
110 120 130 140 150
AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS
160 170 180 190 200
CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE
210 220 230 240 250
LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER
260 270 280 290
TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS
Length:298
Mass (Da):32,798
Last modified:July 11, 2002 - v2
Checksum:iB2EAAEE531BEC101
GO
Isoform 1 (identifier: Q96CA5-2) [UniParc]FASTAAdd to basket
Also known as: Livin beta

The sequence of this isoform differs from the canonical sequence as follows:
     216-233: Missing.

Show »
Length:280
Mass (Da):30,866
Checksum:i630BE9C0737F7952
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020253223E → Q.1 PublicationCorresponds to variant rs1077019dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002459216 – 233Missing in isoform 1. 2 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF301009 mRNA. Translation: AAG37878.1.
AJ309298 Genomic DNA. Translation: CAC37337.1.
AJ309298 Genomic DNA. Translation: CAC37338.1.
AF311388 mRNA. Translation: AAG33622.1.
AY358835 mRNA. Translation: AAQ89194.1.
AY358836 mRNA. Translation: AAQ89195.1.
AL121827 Genomic DNA. No translation available.
BC014475 mRNA. Translation: AAH14475.1.
CCDSiCCDS13512.1. [Q96CA5-2]
CCDS13513.1. [Q96CA5-1]
PIRiJC7568.
RefSeqiNP_071444.1. NM_022161.3. [Q96CA5-2]
NP_647478.1. NM_139317.2. [Q96CA5-1]
UniGeneiHs.256126.

Genome annotation databases

EnsembliENST00000217169; ENSP00000217169; ENSG00000101197. [Q96CA5-1]
ENST00000342412; ENSP00000345213; ENSG00000101197. [Q96CA5-2]
GeneIDi79444.
KEGGihsa:79444.
UCSCiuc002yei.5. human. [Q96CA5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF301009 mRNA. Translation: AAG37878.1.
AJ309298 Genomic DNA. Translation: CAC37337.1.
AJ309298 Genomic DNA. Translation: CAC37338.1.
AF311388 mRNA. Translation: AAG33622.1.
AY358835 mRNA. Translation: AAQ89194.1.
AY358836 mRNA. Translation: AAQ89195.1.
AL121827 Genomic DNA. No translation available.
BC014475 mRNA. Translation: AAH14475.1.
CCDSiCCDS13512.1. [Q96CA5-2]
CCDS13513.1. [Q96CA5-1]
PIRiJC7568.
RefSeqiNP_071444.1. NM_022161.3. [Q96CA5-2]
NP_647478.1. NM_139317.2. [Q96CA5-1]
UniGeneiHs.256126.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OXNX-ray2.20A/B/C/D/E63-179[»]
1OXQX-ray2.30A/B/C/D/E63-179[»]
1OY7X-ray2.70A/B/C/D/E63-179[»]
1TW6X-ray1.71A/B63-172[»]
2I3HX-ray1.62A/B63-172[»]
2I3IX-ray2.30A/B63-172[»]
3F7GX-ray2.30A/B/C/D/E63-179[»]
3F7HX-ray1.80A/B63-172[»]
3F7IX-ray1.90A/B63-172[»]
3GT9X-ray1.70A/B63-172[»]
3GTAX-ray1.70A/B63-172[»]
3UW5X-ray1.71A/B63-159[»]
4AUQX-ray2.18B/E239-298[»]
ProteinModelPortaliQ96CA5.
SMRiQ96CA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122670. 32 interactors.
DIPiDIP-33486N.
IntActiQ96CA5. 27 interactors.
MINTiMINT-147031.
STRINGi9606.ENSP00000217169.

Chemistry databases

BindingDBiQ96CA5.
ChEMBLiCHEMBL1075127.
GuidetoPHARMACOLOGYi2794.

Protein family/group databases

MEROPSiI32.007.

PTM databases

iPTMnetiQ96CA5.
PhosphoSitePlusiQ96CA5.

Polymorphism and mutation databases

BioMutaiBIRC7.
DMDMi21759008.

Proteomic databases

MaxQBiQ96CA5.
PaxDbiQ96CA5.
PeptideAtlasiQ96CA5.
PRIDEiQ96CA5.

Protocols and materials databases

DNASUi79444.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217169; ENSP00000217169; ENSG00000101197. [Q96CA5-1]
ENST00000342412; ENSP00000345213; ENSG00000101197. [Q96CA5-2]
GeneIDi79444.
KEGGihsa:79444.
UCSCiuc002yei.5. human. [Q96CA5-1]

Organism-specific databases

CTDi79444.
DisGeNETi79444.
GeneCardsiBIRC7.
HGNCiHGNC:13702. BIRC7.
HPAiCAB037025.
CAB037119.
HPA047850.
MIMi605737. gene.
neXtProtiNX_Q96CA5.
OpenTargetsiENSG00000101197.
PharmGKBiPA25364.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1101. Eukaryota.
ENOG410YPNM. LUCA.
GeneTreeiENSGT00500000044782.
HOGENOMiHOG000232059.
HOVERGENiHBG099136.
InParanoidiQ96CA5.
KOiK16061.
OMAiFPSCQFL.
OrthoDBiEOG091G0CXH.
PhylomeDBiQ96CA5.
TreeFamiTF105356.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101197-MONOMER.
SignaLinkiQ96CA5.
SIGNORiQ96CA5.

Miscellaneous databases

EvolutionaryTraceiQ96CA5.
GeneWikiiBIRC7.
GenomeRNAii79444.
PMAP-CutDBQ96CA5.
PROiQ96CA5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101197.
CleanExiHS_BIRC7.
ExpressionAtlasiQ96CA5. baseline and differential.
GenevisibleiQ96CA5. HS.

Family and domain databases

CDDicd00022. BIR. 1 hit.
Gene3Di1.10.1170.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001370. BIR_rpt.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00653. BIR. 1 hit.
[Graphical view]
SMARTiSM00238. BIR. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS01282. BIR_REPEAT_1. 1 hit.
PS50143. BIR_REPEAT_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIRC7_HUMAN
AccessioniPrimary (citable) accession number: Q96CA5
Secondary accession number(s): Q9BQV0, Q9H2A8, Q9HAP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.