ID   PP14B_HUMAN             Reviewed;         147 AA.
AC   Q96C90; Q504S7; Q7KZD7;
DT   09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   09-NOV-2004, sequence version 3.
DT   24-JAN-2024, entry version 164.
DE   RecName: Full=Protein phosphatase 1 regulatory subunit 14B;
DE   AltName: Full=Phospholipase C-beta-3 neighbouring gene protein;
GN   Name=PPP1R14B; Synonyms=PLCB3N, PNG;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=8838322; DOI=10.1006/geno.1996.0063;
RA   Lagercrantz J., Carson E., Larsson C., Nordenskjoeld M., Weber G.;
RT   "Isolation and characterization of a novel gene close to the human
RT   phosphoinositide-specific phospholipase C beta 3 gene on chromosomal region
RT   11q13.";
RL   Genomics 31:380-384(1996).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=16964243; DOI=10.1038/nbt1240;
RA   Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT   "A probability-based approach for high-throughput protein phosphorylation
RT   analysis and site localization.";
RL   Nat. Biotechnol. 24:1285-1292(2006).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [6]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RX   PubMed=19413330; DOI=10.1021/ac9004309;
RA   Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT   "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT   refined SCX-based approach.";
RL   Anal. Chem. 81:4493-4501(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [10]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RX   PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA   Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA   Giglione C.;
RT   "Comparative large-scale characterisation of plant vs. mammal proteins
RT   reveals similar and idiosyncratic N-alpha acetylation features.";
RL   Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN   [11]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [12]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; TYR-29 AND SER-32, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Inhibitor of PPP1CA. Has over 50-fold higher inhibitory
CC       activity when phosphorylated (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
CC       {ECO:0000269|PubMed:8838322}.
CC   -!- PTM: Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown
CC       Ser is also phosphorylated by PKC (in vitro) (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BT009914; AAP88916.1; -; mRNA.
DR   EMBL; BC014522; AAH14522.3; -; mRNA.
DR   EMBL; BC094817; AAH94817.1; -; mRNA.
DR   CCDS; CCDS31596.1; -.
DR   RefSeq; NP_619634.1; NM_138689.2.
DR   AlphaFoldDB; Q96C90; -.
DR   BioGRID; 117696; 28.
DR   IntAct; Q96C90; 5.
DR   MINT; Q96C90; -.
DR   STRING; 9606.ENSP00000310117; -.
DR   GlyGen; Q96C90; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q96C90; -.
DR   PhosphoSitePlus; Q96C90; -.
DR   BioMuta; PPP1R14B; -.
DR   DMDM; 55583975; -.
DR   EPD; Q96C90; -.
DR   jPOST; Q96C90; -.
DR   MassIVE; Q96C90; -.
DR   MaxQB; Q96C90; -.
DR   PaxDb; 9606-ENSP00000310117; -.
DR   PeptideAtlas; Q96C90; -.
DR   ProteomicsDB; 76165; -.
DR   Pumba; Q96C90; -.
DR   TopDownProteomics; Q96C90; -.
DR   Antibodypedia; 43962; 74 antibodies from 21 providers.
DR   DNASU; 26472; -.
DR   Ensembl; ENST00000309318.8; ENSP00000310117.3; ENSG00000173457.11.
DR   GeneID; 26472; -.
DR   KEGG; hsa:26472; -.
DR   MANE-Select; ENST00000309318.8; ENSP00000310117.3; NM_138689.3; NP_619634.1.
DR   UCSC; uc001nza.4; human.
DR   AGR; HGNC:9057; -.
DR   CTD; 26472; -.
DR   DisGeNET; 26472; -.
DR   GeneCards; PPP1R14B; -.
DR   HGNC; HGNC:9057; PPP1R14B.
DR   HPA; ENSG00000173457; Low tissue specificity.
DR   MIM; 601140; gene.
DR   neXtProt; NX_Q96C90; -.
DR   OpenTargets; ENSG00000173457; -.
DR   PharmGKB; PA33624; -.
DR   VEuPathDB; HostDB:ENSG00000173457; -.
DR   eggNOG; KOG0824; Eukaryota.
DR   GeneTree; ENSGT00950000182985; -.
DR   HOGENOM; CLU_114155_1_0_1; -.
DR   InParanoid; Q96C90; -.
DR   OMA; DMDSDEH; -.
DR   OrthoDB; 5321897at2759; -.
DR   PhylomeDB; Q96C90; -.
DR   TreeFam; TF105546; -.
DR   PathwayCommons; Q96C90; -.
DR   SignaLink; Q96C90; -.
DR   SIGNOR; Q96C90; -.
DR   BioGRID-ORCS; 26472; 122 hits in 1093 CRISPR screens.
DR   ChiTaRS; PPP1R14B; human.
DR   GeneWiki; PPP1R14B; -.
DR   GenomeRNAi; 26472; -.
DR   Pharos; Q96C90; Tbio.
DR   PRO; PR:Q96C90; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q96C90; Protein.
DR   Bgee; ENSG00000173457; Expressed in cortical plate and 95 other cell types or tissues.
DR   ExpressionAtlas; Q96C90; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR   Gene3D; 1.10.150.220; CPI-17; 1.
DR   InterPro; IPR008025; CPI-17.
DR   InterPro; IPR036658; CPI-17_sf.
DR   PANTHER; PTHR16188; PROTEIN PHOSPHATASE 1 INHIBITOR POTENTIATED BY PROTEIN KINASE C; 1.
DR   PANTHER; PTHR16188:SF5; PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 14B; 1.
DR   Pfam; PF05361; PP1_inhibitor; 1.
DR   SUPFAM; SSF81790; Myosin phosphatase inhibitor 17kDa protein, CPI-17; 1.
DR   Genevisible; Q96C90; HS.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Cytoplasm; Phosphoprotein;
KW   Protein phosphatase inhibitor; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0007744|PubMed:19413330,
FT                   ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378"
FT   CHAIN           2..147
FT                   /note="Protein phosphatase 1 regulatory subunit 14B"
FT                   /id="PRO_0000071490"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          61..103
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0007744|PubMed:19413330,
FT                   ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         29
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:16964243,
FT                   ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231,
FT                   ECO:0007744|PubMed:23186163"
FT   MOD_RES         57
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q62084"
SQ   SEQUENCE   147 AA;  15911 MW;  2932A4EA9F312451 CRC64;
     MADSGTAGGA ALAAPAPGPG SGGPGPRVYF QSPPGAAGEG PGGADDEGPV RRQGKVTVKY
     DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDARA ARVKELLVDC
     YKPTEAFISG LLDKIRGMQK LSTPQKK
//