Q96C90 (PP14B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 14B

Alternative name(s):
Phospholipase C-beta-3 neighbouring gene protein

Gene names

Name:	PPP1R14B
Synonyms:	PLCB3N, PNG

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Ubiquitous. Expressed at low levels. Ref.3
Post-translational modification	Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown Ser is also phosphorylated by PKC (in vitro) By similarity.
Sequence similarities	Belongs to the PP1 inhibitor family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Molecular function	Protein phosphatase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein phosphatase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW protein phosphatase type 1 regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Protein phosphatase 1 regulatory subunit 14B		PRO_0000071490
Regions
	Coiled coil	61 – 103	43	Potential
Amino acid modifications
	Modified residue	32	1	Phosphoserine Ref.4 Ref.5 Ref.7
	Modified residue	57	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q96C90 [UniParc]. Last modified November 9, 2004. Version 3. Checksum: 2932A4EA9F312451	FASTA	147	15,911
	10 20 30 40 50 60 MADSGTAGGA ALAAPAPGPG SGGPGPRVYF QSPPGAAGEG PGGADDEGPV RRQGKVTVKY 70 80 90 100 110 120 DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDARA ARVKELLVDC 130 140 YKPTEAFISG LLDKIRGMQK LSTPQKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[3]	"Isolation and characterization of a novel gene close to the human phosphoinositide-specific phospholipase C beta 3 gene on chromosomal region 11q13." Lagercrantz J., Carson E., Larsson C., Nordenskjoeld M., Weber G. Genomics 31:380-384(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT009914 mRNA. Translation: AAP88916.1. BC014522 mRNA. Translation: AAH14522.3. BC094817 mRNA. Translation: AAH94817.1.
IPI	IPI00398922.
RefSeq	NP_619634.1. NM_138689.2.
UniGene	Hs.523760. Hs.596713.
3D structure databases
ProteinModelPortal	Q96C90.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3051633.
STRING	9606.ENSP00000310117.
PTM databases
PhosphoSite	Q96C90.
Polymorphism databases
DMDM	55583975.
Proteomic databases
PaxDb	Q96C90.
PRIDE	Q96C90.
Protocols and materials databases
DNASU	26472.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309318; ENSP00000310117; ENSG00000173457.
GeneID	26472.
KEGG	hsa:26472.
UCSC	uc001nza.3. human.
Organism-specific databases
CTD	26472.
GeneCards	GC11M064011.
HGNC	HGNC:9057. PPP1R14B.
MIM	601140. gene.
neXtProt	NX_Q96C90.
PharmGKB	PA33624.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG124396.
HOGENOM	HOG000231993.
HOVERGEN	HBG054419.
InParanoid	Q96C90.
OMA	NLEEWII.
OrthoDB	EOG4BVRW6.
PhylomeDB	Q96C90.
Gene expression databases
ArrayExpress	Q96C90.
Bgee	Q96C90.
CleanEx	HS_PPP1R14B.
Genevestigator	Q96C90.
GermOnline	ENSG00000173457. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.220. 1 hit.
InterPro	IPR008025. PP1_inhibitor. [Graphical view]
PANTHER	PTHR16188. PTHR16188. 1 hit.
Pfam	PF05361. PP1_inhibitor. 1 hit. [Graphical view]
SUPFAM	SSF81790. PP1_inhibitor. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	26472.
NextBio	48728.
SOURCE	Search...

Entry information

Entry name

PP14B_HUMAN

Accession

Primary (citable) accession number: Q96C90
Secondary accession number(s): Q504S7, Q7KZD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	November 9, 2004
Last modified:	May 1, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.