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Q96C24 (SYTL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene names
Name:SYTL4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner By similarity.

Subunit structure

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A By similarity.

Subcellular location

Membrane; Peripheral membrane protein By similarity. Cell membrane; Peripheral membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Peripheral membrane protein By similarity. Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles By similarity.

Sequence similarities

Contains 2 C2 domains.

Contains 1 FYVE-type zinc finger.

Contains 1 RabBD (Rab-binding) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processexocytosis

Inferred from electronic annotation. Source: Ensembl

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

multivesicular body sorting pathway

Inferred from mutant phenotype PubMed 19966785. Source: UniProtKB

negative regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of exocytosis

Inferred from mutant phenotype PubMed 19966785. Source: UniProtKB

positive regulation of protein secretion

Inferred from mutant phenotype PubMed 19966785. Source: UniProtKB

   Cellular_componentcentrosome

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

endosome

Inferred from direct assay PubMed 19966785. Source: UniProtKB

extrinsic component of membrane

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

secretory granule

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle

Inferred from electronic annotation. Source: InterPro

transport vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionneurexin family protein binding

Inferred from sequence or structural similarity. Source: UniProtKB

phospholipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

transporter activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96C24-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96C24-2)

The sequence of this isoform differs from the canonical sequence as follows:
     336-657: Missing.
     658-671: LQLRSSMAKQKLGL → VSSIRSVVTGMLGY
Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 671671Synaptotagmin-like protein 4
PRO_0000190216

Regions

Domain4 – 122119RabBD
Domain358 – 462105C2 1
Domain513 – 617105C2 2
Zinc finger63 – 10543FYVE-type
Compositional bias305 – 3117Poly-Glu

Amino acid modifications

Modified residue2041Phosphoserine Ref.7
Modified residue2171Phosphoserine Ref.7
Modified residue2891Phosphoserine Ref.7

Natural variations

Alternative sequence336 – 657322Missing in isoform 2.
VSP_015237
Alternative sequence658 – 67114LQLRS…QKLGL → VSSIRSVVTGMLGY in isoform 2.
VSP_015238
Natural variant4201I → V. Ref.1 Ref.2 Ref.4
Corresponds to variant rs2022039 [ dbSNP | Ensembl ].
VAR_016076

Experimental info

Sequence conflict441L → P in CAI46126. Ref.2
Sequence conflict1601T → A in BAC04287. Ref.1
Sequence conflict2511V → A in CAI46126. Ref.2
Sequence conflict2871S → G in BAC04287. Ref.1

Secondary structure

...................................... 671
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: 04152119330EAB84

FASTA67176,024
        10         20         30         40         50         60 
MSELLDLSFL SEEEKDLILS VLQRDEEVRK ADEKRIRRLK NELLEIKRKG AKRGSQHYSD 

        70         80         90        100        110        120 
RTCARCQESL GRLSPKTNTC RGCNHLVCRD CRIQESNGTW RCKVCAKEIE LKKATGDWFY 

       130        140        150        160        170        180 
DQKVNRFAYR TGSEIIRMSL RHKPAVSKRE TVGQSLLHQT QMGDIWPGRK IIQERQKEPS 

       190        200        210        220        230        240 
VLFEVPKLKS GKSALEAESE SLDSFTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE 

       250        260        270        280        290        300 
TQPGGQNVVF VDEGEMIFKK NTRKILRPSE YTKSVIDLRP EDVVHESGSL GDRSKSVPGL 

       310        320        330        340        350        360 
NVDMEEEEEE EDIDHLVKLH RQKLARSSMQ SGSSMSTIGS MMSIYSEAGD FGNIFVTGRI 

       370        380        390        400        410        420 
AFSLKYEQQT QSLVVHVKEC HQLAYADEAK KRSNPYVKTY LLPDKSRQGK RKTSIKRDTI 

       430        440        450        460        470        480 
NPLYDETLRY EIPESLLAQR TLQFSVWHHG RFGRNTFLGE AEIQMDSWKL DKKLDHCLPL 

       490        500        510        520        530        540 
HGKISAESPT GLPSHKGELV VSLKYIPASK TPVGGDRKKS KGGEGGELQV WIKEAKNLTA 

       550        560        570        580        590        600 
AKAGGTSDSF VKGYLLPMRN KASKRKTPVM KKTLNPHYNH TFVYNGVRLE DLQHMCLELT 

       610        620        630        640        650        660 
VWDREPLASN DFLGGVRLGV GTGISNGEVV DWMDSTGEEV SLWQKMRQYP GSWAEGTLQL 

       670 
RSSMAKQKLG L 

« Hide

Isoform 2 [UniParc].

Checksum: 815C9B7DC933119E
Show »

FASTA34939,813

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-420.
Tissue: Adrenal gland.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-420.
Tissue: Endometrium and Spinal cord.
[3]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-420.
Tissue: Uterus.
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-204; SER-217 AND SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Solution structure of the RING domain of the synaptotagmin-like protein 4."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 43-105.
[9]"Crystal structure of a C2 domain from human synaptotagmin-like protein 4."
New York structural genomix research consortium (NYSGXRC)
Submitted (DEC-2008) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 352-488.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK094110 mRNA. Translation: BAC04287.1.
BX537410 mRNA. Translation: CAD97652.1.
AL832596 mRNA. Translation: CAI46126.1.
BC014913 mRNA. Translation: AAH14913.1.
Z73900, AL391688 Genomic DNA. Translation: CAI42004.1.
Z73900, AL391688 Genomic DNA. Translation: CAI42005.1.
RefSeqNP_001123368.1. NM_001129896.2.
NP_001167539.1. NM_001174068.1.
NP_542775.2. NM_080737.2.
XP_005262283.1. XM_005262226.1.
XP_005262286.1. XM_005262229.1.
UniGeneHs.592224.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CSZNMR-A43-105[»]
3FDWX-ray2.20A/B352-488[»]
ProteinModelPortalQ96C24.
SMRQ96C24. Positions 10-120, 354-662.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125120. 10 interactions.
IntActQ96C24. 3 interactions.
STRING9606.ENSP00000263033.

Chemistry

DrugBankDB00047. Insulin Glargine recombinant.
DB00046. Insulin Lyspro recombinant.
DB00030. Insulin recombinant.
DB00071. Insulin, porcine.

PTM databases

PhosphoSiteQ96C24.

Polymorphism databases

DMDM317373281.

Proteomic databases

PaxDbQ96C24.
PRIDEQ96C24.

Protocols and materials databases

DNASU94121.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263033; ENSP00000263033; ENSG00000102362. [Q96C24-1]
ENST00000276141; ENSP00000276141; ENSG00000102362. [Q96C24-1]
ENST00000372981; ENSP00000362072; ENSG00000102362. [Q96C24-2]
ENST00000372989; ENSP00000362080; ENSG00000102362. [Q96C24-1]
ENST00000455616; ENSP00000390252; ENSG00000102362. [Q96C24-1]
GeneID94121.
KEGGhsa:94121.
UCSCuc004egd.4. human. [Q96C24-1]
uc004egg.4. human. [Q96C24-2]

Organism-specific databases

CTD94121.
GeneCardsGC0XM099929.
H-InvDBHIX0016918.
HGNCHGNC:15588. SYTL4.
HPAHPA001475.
HPA001589.
MIM300723. gene.
neXtProtNX_Q96C24.
PharmGKBPA37987.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293068.
HOVERGENHBG054255.
InParanoidQ96C24.
KOK17598.
OrthoDBEOG7JMGDC.
PhylomeDBQ96C24.
TreeFamTF341184.

Gene expression databases

ArrayExpressQ96C24.
BgeeQ96C24.
CleanExHS_SYTL4.
GenevestigatorQ96C24.

Family and domain databases

Gene3D2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028694. SYTL4.
IPR010911. Znf_FYVE-typ.
IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERPTHR10024:SF9. PTHR10024:SF9. 1 hit.
PfamPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
PRINTSPR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSYTL4. human.
EvolutionaryTraceQ96C24.
GeneWikiSYTL4.
GenomeRNAi94121.
NextBio78402.
PROQ96C24.
SOURCESearch...

Entry information

Entry nameSYTL4_HUMAN
AccessionPrimary (citable) accession number: Q96C24
Secondary accession number(s): Q5H9J3 expand/collapse secondary AC list , Q5JPG8, Q8N9P4, Q9H4R0, Q9H4R1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM