Reviewed, UniProtKB/Swiss-Prot Q96BZ4 (PLD4_HUMAN)

Names and origin

Protein names

Recommended name:
    Phospholipase D4
      Short name=PLD 4
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 4
    Phosphatidylcholine-hydrolyzing phospholipase D4

Gene names

Name:	PLD4
Synonyms:	C14orf175
ORF Names:	UNQ2488/PRO5775

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Phospholipase D4		PRO_0000280333
Regions
	Transmembrane	31 – 51	21	Potential
	Domain	209 – 236	28	PLD phosphodiesterase 1
	Domain	423 – 449	27	PLD phosphodiesterase 2
Sites
	Active site	214	1	Potential
	Active site	216	1	Potential
	Active site	221	1	Potential
Amino acid modifications
	Modified residue	472	1	Phosphothreonine Ref.3
Natural variations
	Natural variant	27	1	E → Q: dbSNP rs2841280.		VAR_031119
	Natural variant	135	1	V → M: dbSNP rs3803295.		VAR_031120

Sequences

	Sequence		Length	Mass (Da)
	Q96BZ4-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: D7F820C83131FE26	FASTA	506	55,626
	10 20 30 40 50 60 MLKPLWKAAV APTWPCSMPP RRPWDREAGT LQVLGALAVL WLGSVALICL LWQVPRPPTW 70 80 90 100 110 120 GQVQPKDVPR SWEHGSSPAW EPLEAEARQQ RDSCQLVLVE SIPQDLPSAA GSPSAQPLGQ 130 140 150 160 170 180 AWLQLLDTAQ ESVHVASYYW SLTGPDIGVN DSSSQLGEAL LQKLQQLLGR NISLAVATSS 190 200 210 220 230 240 PTLARTSTDL QVLAARGAHV RQVPMGRLTR GVLHSKFWVV DGRHIYMGSA NMDWRSLTQV 250 260 270 280 290 300 KELGAVIYNC SHLAQDLEKT FQTYWVLGVP KAVLPKTWPQ NFSSHFNRFQ PFHGLFDGVP 310 320 330 340 350 360 TTAYFSASPP ALCPQGRTRD LEALLAVMGS AQEFIYASVM EYFPTTRFSH PPRYWPVLDN 370 380 390 400 410 420 ALRAAAFGKG VRVRLLVGCG LNTDPTMFPY LRSLQALSNP AANVSVDVKV FIVPVGNHSN 430 440 450 460 470 480 IPFSRVNHSK FMVTEKAAYI GTSNWSEDYF SSTAGVGLVV TQSPGAQPAG ATVQEQLRQL 490 500 FERDWSSRYA VGLDGQAPGQ DCVWQG

References

[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[3]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-472, MASS SPECTROMETRY. Tissue: T-cell.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358843 mRNA. Translation: AAQ89202.1. Different initiation. BC015003 mRNA. Translation: AAH15003.2.
IPI	IPI00060310.
RefSeq	NP_620145.2.
UniGene	Hs.407101
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q96BZ4.
PTM databases
PhosphoSite	Q96BZ4.
Proteomic databases
PRIDE	Q96BZ4.
Genome annotation databases
Ensembl	ENST00000392593; ENSP00000376372; ENSG00000166428; Homo sapiens. [Genome view]
GeneID	122618.
KEGG	hsa:122618.
UCSC	uc001ypu.1. human.
Organism-specific databases
CTD	122618.
GeneCards	GC14P104464.
HGNC	HGNC:23792. PLD4.
PharmGKB	PA134861676.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q96BZ4.
OMA	VNDSSSQ.
Enzyme and pathway databases
BRENDA	3.1.4.4. 247.
Gene expression databases
ArrayExpress	Q96BZ4.
Bgee	Q96BZ4.
CleanEx	HS_PLD4.
Genevestigator	Q96BZ4.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00122. Choline.
NextBio	80933.

Entry information

Entry name

PLD4_HUMAN

Accession

Primary (citable) accession number: Q96BZ4
Secondary accession number(s): Q6UWD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 16, 2006
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.