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Q96BY2 (MOAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modulator of apoptosis 1

Short name=MAP-1
Short name=MAP1
Alternative name(s):
Paraneoplastic antigen Ma4
Gene names
Name:MOAP1
Synonyms:PNMA4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for death receptor-dependent apoptosis. When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Ref.5

Subunit structure

Homodimer. Under normal circumstances, held in an inactive conformation by an intramolecular interaction. Binding to RASSF1 isoform A(RASSF1A) relieves this inhibitory interaction and allows further binding to BAX. Binds also to BCL2 and BCLX. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Interacts with TRIM39. Interacts with RASSF6 By similarity. Ref.5 Ref.7

Tissue specificity

Widely expressed, with high levels in heart and brain. Ref.6

Domain

The BH3-like domain is required for association with BAX and for mediating apoptosis. The three BH domains (BH1, BH2, and BH3) of BAX are all required for mediating protein-protein interaction.

Post-translational modification

Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39. Ref.8

Sequence similarities

Belongs to the PNMA family.

Sequence caution

The sequence BAB14788.1 differs from that shown. Reason: Frameshift at position 102.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Modulator of apoptosis 1
PRO_0000155205

Regions

Region120 – 1278BH3-like
Region202 – 2054RASSF1-binding

Experimental info

Mutagenesis120 – 1278Missing: Abrogates interaction with BAX, resulting in a nonapoptotic protein. Ref.1
Mutagenesis1201L → E: Weakened interaction with BAX, resulting in a nonapoptotic protein. Ref.1
Mutagenesis125 – 1273GHE → VLA: Abrogates interaction with BAX, resulting in a nonapoptotic protein. Ref.1
Mutagenesis161 – 1666KYKKLR → AYAALA: No effect on RASSF1-binding.
Mutagenesis178 – 1803EEE → AAA: No effect on RASSF1-binding; interacts with BAX in the absence of RASSF1.
Mutagenesis202 – 2054KRRR → AAAA: Loss of RASSF1-binding; interacts with BAX in the absence of RASSF1. Ref.5
Sequence conflict2441T → A in BAB14788. Ref.2
Sequence conflict2581Y → H in BAB14788. Ref.2
Sequence conflict2591Q → H in AAG31786. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q96BY2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5310142AC02B563C

FASTA35139,513
        10         20         30         40         50         60 
MTLRLLEDWC RGMDMNPRKA LLIAGISQSC SVAEIEEALQ AGLAPLGEYR LLGRMFRRDE 

        70         80         90        100        110        120 
NRKVALVGLT AETSHALVPK EIPGKGGIWR VIFKPPDPDN TFLSRLNEFL AGEGMTVGEL 

       130        140        150        160        170        180 
SRALGHENGS LDPEQGMIPE MWAPMLAQAL EALQPALQCL KYKKLRVFSG RESPEPGEEE 

       190        200        210        220        230        240 
FGRWMFHTTQ MIKAWQVPDV EKRRRLLESL RGPALDVIRV LKINNPLITV DECLQALEEV 

       250        260        270        280        290        300 
FGVTDNPREL QVKYLTTYQK DEEKLSAYVL RLEPLLQKLV QRGAIERDAV NQARLDQVIA 

       310        320        330        340        350 
GAVHKTIRRE LNLPEDGPAP GFLQLLVLIK DYEAAEEEEA LLQAILEGNF T 

« Hide

References

« Hide 'large scale' references
[1]"MAP-1, a novel proapoptotic protein containing a BH3-like motif that associates with Bax through its Bcl-2 homology domains."
Tan K.O., Tan K.M.L., Chan S.-L., Yee K.S.Y., Bevort M., Ang K.C., Yu V.C.
J. Biol. Chem. 276:2802-2807(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF LEU-120; 120-LEU--ARG-127 AND 125-GLY--GLU-127.
Tissue: Cerebellum.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Retinoblastoma and Tongue.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[5]"The tumor suppressor RASSF1A and MAP-1 link death receptor signaling to Bax conformational change and cell death."
Baksh S., Tommasi S., Fenton S., Yu V.C., Martins L.M., Pfeifer G.P., Latif F., Downward J., Neel B.G.
Mol. Cell 18:637-650(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RASSF1, INTRAMOLECULAR INTERACTION, MUTAGENESIS OF 161-LYS--ARG-166; 178-GLU--ALA-181 AND 202-LYS--ARG-205, FUNCTION.
[6]"Paraneoplastic antigen-like 5 gene (PNMA5) is preferentially expressed in the association areas in a primate specific manner."
Takaji M., Komatsu Y., Watakabe A., Hashikawa T., Yamamori T.
Cereb. Cortex 19:2865-2879(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"TRIM39 is a MOAP-1-binding protein that stabilizes MOAP-1 through inhibition of its poly-ubiquitination process."
Lee S.S., Fu N.Y., Sukumaran S.K., Wan K.F., Wan Q., Yu V.C.
Exp. Cell Res. 315:1313-1325(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRIM39.
[8]"The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1."
Huang N.J., Zhang L., Tang W., Chen C., Yang C.S., Kornbluth S.
J. Cell Biol. 197:361-367(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION BY APC/C-CDH1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF305550 mRNA. Translation: AAG31786.1.
AK024029 mRNA. Translation: BAB14788.1. Sequence problems.
AK315522 mRNA. Translation: BAG37903.1.
CH471061 Genomic DNA. Translation: EAW81516.1.
BC015044 mRNA. Translation: AAH15044.1.
CCDSCCDS9908.1.
RefSeqNP_071434.2. NM_022151.4.
UniGeneHs.24719.

3D structure databases

ProteinModelPortalQ96BY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122069. 13 interactions.
IntActQ96BY2. 9 interactions.
MINTMINT-1438458.

PTM databases

PhosphoSiteQ96BY2.

Polymorphism databases

DMDM37999755.

Proteomic databases

PaxDbQ96BY2.
PRIDEQ96BY2.

Protocols and materials databases

DNASU64112.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000298894; ENSP00000298894; ENSG00000165943.
ENST00000556883; ENSP00000451594; ENSG00000165943.
GeneID64112.
KEGGhsa:64112.
UCSCuc001ybj.3. human.

Organism-specific databases

CTD64112.
GeneCardsGC14M093648.
HGNCHGNC:16658. MOAP1.
HPAHPA000939.
MIM609485. gene.
neXtProtNX_Q96BY2.
PharmGKBPA134908381.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145999.
HOGENOMHOG000013079.
HOVERGENHBG052488.
InParanoidQ96BY2.
OMAIPEMWAP.
OrthoDBEOG708VZW.
PhylomeDBQ96BY2.
TreeFamTF335054.

Gene expression databases

BgeeQ96BY2.
CleanExHS_MOAP1.
GenevestigatorQ96BY2.

Family and domain databases

InterProIPR026523. PNMA.
[Graphical view]
PANTHERPTHR23095. PTHR23095. 1 hit.
PfamPF14893. PNMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMOAP1. human.
GeneWikiMOAP1.
GenomeRNAi64112.
NextBio65976.
PROQ96BY2.
SOURCESearch...

Entry information

Entry nameMOAP1_HUMAN
AccessionPrimary (citable) accession number: Q96BY2
Secondary accession number(s): B2RDF6, Q9H833, Q9HAS1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM