Reviewed, UniProtKB/Swiss-Prot Q96BT7 (ALKB8_HUMAN)

Names and origin

Protein names

Recommended name:
    Alkylated DNA repair protein alkB homolog 8

Gene names

Name:

ALKBH8

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May inhibit apoptosis and promote cell survival and angiogenesis. Ref.5
Sequence similarities	Belongs to the alkB family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96BT7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96BT7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-238: GIPAH → DCHGF
     239-664: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: Q96BT7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     215-224: GYIKHKPDQM → AEKNLEVGIH
     225-664: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 664

664

Alkylated DNA repair protein alkB homolog 8

PRO_0000337125

Regions

Domain

43 – 120

78

RRM

Natural variations

Alternative sequence

215 – 224

10

GYIKHKPDQM → AEKNLEVGIH in isoform 3.

VSP_033925

Alternative sequence

225 – 664

440

Missing in isoform 3.

VSP_033926

Alternative sequence

234 – 238

5

GIPAH → DCHGF in isoform 2.

VSP_033927

Alternative sequence

239 – 664

426

Missing in isoform 2.

VSP_033928

Experimental info

Sequence conflict

210

1

K → R in BAC04566. Ref.1

Secondary structure

1

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664

Helix Strand Turn

Details...

Beta strand

26 – 28

3

Helix

29 – 33

5

Beta strand

43 – 48

6

Helix

52 – 54

3

Helix

58 – 68

11

Beta strand

71 – 75

5

Beta strand

83 – 89

7

Helix

90 – 99

10

Turn

100 – 102

3

Beta strand

104 – 106

3

Beta strand

112 – 114

3

Beta strand

116 – 121

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 4BE595D6757C2A43	FASTA	664	75,208
	10 20 30 40 50 60 MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG GLGNGVSRNQ 70 80 90 100 110 120 LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL NGKEVVDDLG QKITLYLNFV 130 140 150 160 170 180 EKVQWKELRP QALPPGLMVV EEIISSEEEK MLLESVDWTE DTDNQNSQKS LKHRRVKHFG 190 200 210 220 230 240 YEFHYENNNV DKDKPLSGGL PDICESFLEK WLRKGYIKHK PDQMTINQYE PGQGIPAHID 250 260 270 280 290 300 THSAFEDEIV SLSLGSEIVM DFKHPDGIAV PVMLPRRSLL VMTGESRYLW THGITCRKFD 310 320 330 340 350 360 TVQASESLKS GIITSDVGDL TLSKRGLRTS FTFRKVRQTP CNCSYPLVCD SQRKETPPSF 370 380 390 400 410 420 PESDKEASRL EQEYVHQVYE EIAGHFSSTR HTPWPHIVEF LKALPSGSIV ADIGCGNGKY 430 440 450 460 470 480 LGINKELYMI GCDRSQNLVD ICRERQFQAF VCDALAVPVR SGSCDACISI AVIHHFATAE 490 500 510 520 530 540 RRVAALQEIV RLLRPGGKAL IYVWAMEQEY NKQKSKYLRG NRNSQGKKEE MNSDTSVQRS 550 560 570 580 590 600 LVEQMRDMGS RDSASSVPRI NDSQEGGCNS RQVSNSKLPV HVNRTSFYSQ DVLVPWHLKG 610 620 630 640 650 660 NPDKGKPVEP FGPIGSQDPS PVFHRYYHVF REGELEGACR TVSDVRILQS YYDQGNWCVI LQKA
	Isoform 2. Checksum: 24EF6A3A66DCC2F7	FASTA	238	27,440
	10 20 30 40 50 60 MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG GLGNGVSRNQ 70 80 90 100 110 120 LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL NGKEVVDDLG QKITLYLNFV 130 140 150 160 170 180 EKVQWKELRP QALPPGLMVV EEIISSEEEK MLLESVDWTE DTDNQNSQKS LKHRRVKHFG 190 200 210 220 230 YEFHYENNNV DKDKPLSGGL PDICESFLEK WLRKGYIKHK PDQMTINQYE PGQDCHGF
	Isoform 3. Checksum: 6FD66E832509EE87	FASTA	224	25,742
	10 20 30 40 50 60 MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG GLGNGVSRNQ 70 80 90 100 110 120 LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL NGKEVVDDLG QKITLYLNFV 130 140 150 160 170 180 EKVQWKELRP QALPPGLMVV EEIISSEEEK MLLESVDWTE DTDNQNSQKS LKHRRVKHFG 190 200 210 220 YEFHYENNNV DKDKPLSGGL PDICESFLEK WLRKAEKNLE VGIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[5]	"A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression." Shimada K., Nakamura M., Anai S., De Velasco M., Tanaka M., Tsujikawa K., Ouji Y., Konishi N. Cancer Res. 69:3157-3164(2009) [PubMed: 19293182] [Abstract] Cited for: FUNCTION.
[6]	"Solution structure of RNA binding domain in hypothetical protein LOC91801." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 25-125.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AK095523 mRNA. Translation: BAC04566.1.
AP001823 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67090.1.
BC015183 mRNA. Translation: AAH15183.1.

IPI

IPI00026191.
IPI00154533.
IPI00873081.

RefSeq

NP_620130.2.

UniGene

Hs.503763

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CQ2	NMR	-	A	25-125	[»]

SMR

Q96BT7. Positions 25-125.

ModBase

Search...

Protein-protein interaction databases

STRING

Q96BT7.

Genome annotation databases

Ensembl

ENST00000389568; ENSP00000374219; ENSG00000137760; Homo sapiens. [Genome view]
ENST00000417449; ENSP00000397673; ENSG00000137760; Homo sapiens. [Genome view]
ENST00000428149; ENSP00000415885; ENSG00000137760; Homo sapiens. [Genome view]

GeneID

91801.

KEGG

hsa:91801.

UCSC

uc001pjl.1. human.
uc009yxp.1. human.

Organism-specific databases

CTD

91801.

GeneCards

GC11M106879.

HGNC

HGNC:25189. ALKBH8.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q96BT7.

OrthoDB

EOG96DPP0

Gene expression databases

ArrayExpress

Q96BT7.

Bgee

Q96BT7.

CleanEx

HS_ALKBH8.

Genevestigator

Q96BT7.

Family and domain databases

InterPro

IPR015095. DUF1891.
IPR013216. Methyltransf_11.
IPR005123. Oxoglutarate/Fe-dep_oxygenase.
[Graphical view]

Pfam

PF03171. 2OG-FeII_Oxy. 1 hit.
PF09004. DUF1891. 1 hit.
PF08241. Methyltransf_11. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

77461.

Entry information

Entry name

ALKB8_HUMAN

Accession

Primary (citable) accession number: Q96BT7
Secondary accession number(s): Q8N989

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	November 24, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.