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Protein

Centromere protein T

Gene

CENPT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.3 Publications

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • CENP-A containing nucleosome assembly Source: Reactome
  • chromosome organization Source: UniProtKB
  • chromosome segregation Source: UniProtKB
  • kinetochore assembly Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein T
Short name:
CENP-T
Alternative name(s):
Interphase centromere complex protein 22
Gene namesi
Name:CENPT
Synonyms:C16orf56, ICEN22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25787. CENPT.

Subcellular locationi

  • Nucleus
  • Chromosomecentromerekinetochore

  • Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000102901.
PharmGKBiPA142672263.

Polymorphism and mutation databases

BioMutaiCENPT.
DMDMi74760746.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495141 – 561Centromere protein TAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei47PhosphoserineCombined sources1 Publication1
Modified residuei85PhosphothreonineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei345PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1
Modified residuei373PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei386PhosphoserineCombined sources1
Modified residuei397PhosphoserineCombined sources1

Post-translational modificationi

Dynamically phosphorylated at Ser-47 and probably also other sites during the cell cycle. Phosphorylated at Ser-47 during G2 phase, metaphase and anaphase, but not during telophase or G1 phase.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ96BT3.
MaxQBiQ96BT3.
PaxDbiQ96BT3.
PeptideAtlasiQ96BT3.
PRIDEiQ96BT3.

PTM databases

iPTMnetiQ96BT3.
PhosphoSitePlusiQ96BT3.

Expressioni

Gene expression databases

BgeeiENSG00000102901.
CleanExiHS_CENPT.
ExpressionAtlasiQ96BT3. baseline and differential.
GenevisibleiQ96BT3. HS.

Organism-specific databases

HPAiHPA041220.
HPA058036.

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Part of a centromere complex consisting of CENPA, CENPT and CENPW. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Interacts (via N-terminus) with the NDC80 complex (Probable). Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292). Binds DNA.Curated8 Publications

Protein-protein interaction databases

BioGridi123143. 14 interactors.
IntActiQ96BT3. 13 interactors.
MINTiMINT-1376718.
STRINGi9606.ENSP00000219172.

Structurei

3D structure databases

ProteinModelPortaliQ96BT3.
SMRiQ96BT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 421Flexible stalk domainBy similarityAdd BLAST329

Domaini

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.By similarity

Sequence similaritiesi

Belongs to the CENPT family.Curated

Phylogenomic databases

eggNOGiENOG410IFJ0. Eukaryota.
ENOG4111ZWH. LUCA.
GeneTreeiENSGT00390000003044.
HOGENOMiHOG000111545.
HOVERGENiHBG081089.
InParanoidiQ96BT3.
KOiK11512.
OMAiFSFYAKM.
OrthoDBiEOG091G05OQ.
PhylomeDBiQ96BT3.
TreeFamiTF332946.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028255. CENP-T.
IPR032373. CENP-T_N.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR10484:SF80. PTHR10484:SF80. 1 hit.
PfamiPF16171. CENP-T_N. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96BT3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADHNPDSDS TPRTLLRRVL DTADPRTPRR PRSARAGARR ALLETASPRK
60 70 80 90 100
LSGQTRTIAR GRSHGARSVG RSAHIQASGH LEEQTPRTLL KNILLTAPES
110 120 130 140 150
SILMPESVVK PVPAPQAVQP SRQESSCGSL ELQLPELEPP TTLAPGLLAP
160 170 180 190 200
GRRKQRLRLS VFQQGVDQGL SLSQEPQGNA DASSLTRSLN LTFATPLQPQ
210 220 230 240 250
SVQRPGLARR PPARRAVDVG AFLRDLRDTS LAPPNIVLED TQPFSQPMVG
260 270 280 290 300
SPNVYHSLPC TPHTGAEDAE QAAGRKTQSS GPGLQKNSPG KPAQFLAGEA
310 320 330 340 350
EEVNAFALGF LSTSSGVSGE DEVEPLHDGV EEAEKKMEEE GVSVSEMEAT
360 370 380 390 400
GAQGPSRVEE AEGHTEVTEA EGSQGTAEAD GPGASSGDED ASGRAASPES
410 420 430 440 450
ASSTPESLQA RRHHQFLEPA PAPGAAVLSS EPAEPLLVRH PPRPRTTGPR
460 470 480 490 500
PRQDPHKAGL SHYVKLFSFY AKMPMERKAL EMVEKCLDKY FQHLCDDLEV
510 520 530 540 550
FAAHAGRKTV KPEDLELLMR RQGLVTDQVS LHVLVERHLP LEYRQLLIPC
560
AYSGNSVFPA Q
Length:561
Mass (Da):60,423
Last modified:March 1, 2004 - v2
Checksum:i295848C52AAF7DF1
GO
Isoform 2 (identifier: Q96BT3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-299: PGKPAQFLAGE → ECVALVAWSQI
     300-561: Missing.

Note: No experimental confirmation available.
Show »
Length:299
Mass (Da):32,170
Checksum:i437895CBF0AD00DC
GO
Isoform 3 (identifier: Q96BT3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-151: SVGRSAHIQA...TLAPGLLAPG → VSTQPTDPKG...NCPRIFHPDA
     152-561: Missing.

Note: No experimental confirmation available.
Show »
Length:151
Mass (Da):16,434
Checksum:i4A936224C3E78049
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027421115P → L.Corresponds to variant rs12102580dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02045568 – 151SVGRS…LLAPG → VSTQPTDPKGPWLPRGGGLR SSSALEPTLRKSQGRRTDWL LGASPIVCWQIGPYSGQWAL GGTDTSDAAEEHPTNCPRIF HPDA in isoform 3. 1 PublicationAdd BLAST84
Alternative sequenceiVSP_020456152 – 561Missing in isoform 3. 1 PublicationAdd BLAST410
Alternative sequenceiVSP_020457289 – 299PGKPAQFLAGE → ECVALVAWSQI in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_020458300 – 561Missing in isoform 2. 1 PublicationAdd BLAST262

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023173 mRNA. Translation: BAB14445.1.
AK055237 mRNA. Translation: BAB70884.1.
BC007642 mRNA. Translation: AAH07642.1.
BC007864 mRNA. Translation: AAH07864.2.
BC015202 mRNA. Translation: AAH15202.2.
CCDSiCCDS42182.1. [Q96BT3-1]
RefSeqiNP_079358.3. NM_025082.3. [Q96BT3-1]
UniGeneiHs.288382.

Genome annotation databases

EnsembliENST00000440851; ENSP00000400140; ENSG00000102901. [Q96BT3-1]
ENST00000562787; ENSP00000457810; ENSG00000102901. [Q96BT3-1]
GeneIDi80152.
KEGGihsa:80152.
UCSCiuc002eun.4. human. [Q96BT3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023173 mRNA. Translation: BAB14445.1.
AK055237 mRNA. Translation: BAB70884.1.
BC007642 mRNA. Translation: AAH07642.1.
BC007864 mRNA. Translation: AAH07864.2.
BC015202 mRNA. Translation: AAH15202.2.
CCDSiCCDS42182.1. [Q96BT3-1]
RefSeqiNP_079358.3. NM_025082.3. [Q96BT3-1]
UniGeneiHs.288382.

3D structure databases

ProteinModelPortaliQ96BT3.
SMRiQ96BT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123143. 14 interactors.
IntActiQ96BT3. 13 interactors.
MINTiMINT-1376718.
STRINGi9606.ENSP00000219172.

PTM databases

iPTMnetiQ96BT3.
PhosphoSitePlusiQ96BT3.

Polymorphism and mutation databases

BioMutaiCENPT.
DMDMi74760746.

Proteomic databases

EPDiQ96BT3.
MaxQBiQ96BT3.
PaxDbiQ96BT3.
PeptideAtlasiQ96BT3.
PRIDEiQ96BT3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000440851; ENSP00000400140; ENSG00000102901. [Q96BT3-1]
ENST00000562787; ENSP00000457810; ENSG00000102901. [Q96BT3-1]
GeneIDi80152.
KEGGihsa:80152.
UCSCiuc002eun.4. human. [Q96BT3-1]

Organism-specific databases

CTDi80152.
GeneCardsiCENPT.
HGNCiHGNC:25787. CENPT.
HPAiHPA041220.
HPA058036.
MIMi611510. gene.
neXtProtiNX_Q96BT3.
OpenTargetsiENSG00000102901.
PharmGKBiPA142672263.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJ0. Eukaryota.
ENOG4111ZWH. LUCA.
GeneTreeiENSGT00390000003044.
HOGENOMiHOG000111545.
HOVERGENiHBG081089.
InParanoidiQ96BT3.
KOiK11512.
OMAiFSFYAKM.
OrthoDBiEOG091G05OQ.
PhylomeDBiQ96BT3.
TreeFamiTF332946.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSiCENPT. human.
GeneWikiiCENPT.
GenomeRNAii80152.
PROiQ96BT3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102901.
CleanExiHS_CENPT.
ExpressionAtlasiQ96BT3. baseline and differential.
GenevisibleiQ96BT3. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028255. CENP-T.
IPR032373. CENP-T_N.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR10484:SF80. PTHR10484:SF80. 1 hit.
PfamiPF16171. CENP-T_N. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPT_HUMAN
AccessioniPrimary (citable) accession number: Q96BT3
Secondary accession number(s): Q96I29
, Q96IC6, Q96NK9, Q9H901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2004
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.