Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q96BP3 (PPWD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidylprolyl isomerase domain and WD repeat-containing protein 1

EC=5.2.1.8
Alternative name(s):
Spliceosome-associated cyclophilin
Gene names
Name:PPWD1
Synonyms:KIAA0073
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length646 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

Catalytic activity

Peptidylproline (omega=180) = peptidylproline (omega=0).

Subunit structure

Identified in the spliceosome C complex. Ref.5

Subcellular location

Nucleus. Note: Associated with spliceosomal complexes. Ref.10

Sequence similarities

Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.

Contains 1 PPIase cyclophilin-type domain.

Contains 4 WD repeats.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   DomainRepeat
WD repeat
   Molecular functionIsomerase
Rotamase
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA splicing, via spliceosome

Inferred by curator Ref.5. Source: UniProtKB

protein folding

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcatalytic step 2 spliceosome

Inferred from direct assay Ref.5. Source: UniProtKB

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionpeptidyl-prolyl cis-trans isomerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96BP3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96BP3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 646645Peptidylprolyl isomerase domain and WD repeat-containing protein 1
PRO_0000240306

Regions

Repeat88 – 12639WD 1
Repeat131 – 17040WD 2
Repeat221 – 26040WD 3
Repeat278 – 31942WD 4
Domain490 – 645156PPIase cyclophilin-type
Compositional bias12 – 176Poly-Arg

Amino acid modifications

Modified residue21N-acetylalanine Ref.6 Ref.8 Ref.9

Natural variations

Alternative sequence1 – 156156Missing in isoform 2.
VSP_055266

Secondary structure

................................. 646
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 4DEFA1C78F914430

FASTA64673,575
        10         20         30         40         50         60 
MAAESGSDFQ QRRRRRRDPE EPEKTELSER ELAVAVAVSQ ENDEENEERW VGPLPVEATL 

        70         80         90        100        110        120 
AKKRKVLEFE RVYLDNLPSA SMYERSYMHR DVITHVVCTK TDFIITASHD GHVKFWKKIE 

       130        140        150        160        170        180 
EGIEFVKHFR SHLGVIESIA VSSEGALFCS VGDDKAMKVF DVVNFDMINM LKLGYFPGQC 

       190        200        210        220        230        240 
EWIYCPGDAI SSVAASEKST GKIFIYDGRG DNQPLHIFDK LHTSPLTQIR LNPVYKAVVS 

       250        260        270        280        290        300 
SDKSGMIEYW TGPPHEYKFP KNVNWEYKTD TDLYEFAKCK AYPTSVCFSP DGKKIATIGS 

       310        320        330        340        350        360 
DRKVRIFRFV TGKLMRVFDE SLSMFTELQQ MRQQLPDMEF GRRMAVEREL EKVDAVRLIN 

       370        380        390        400        410        420 
IVFDETGHFV LYGTMLGIKV INVETNRCVR ILGKQENIRV MQLALFQGIA KKHRAATTIE 

       430        440        450        460        470        480 
MKASENPVLQ NIQADPTIVC TSFKKNRFYM FTKREPEDTK SADSDRDVFN EKPSKEEVMA 

       490        500        510        520        530        540 
ATQAEGPKRV SDSAIIHTSM GDIHTKLFPV ECPKTVENFC VHSRNGYYNG HTFHRIIKGF 

       550        560        570        580        590        600 
MIQTGDPTGT GMGGESIWGG EFEDEFHSTL RHDRPYTLSM ANAGSNTNGS QFFITVVPTP 

       610        620        630        640 
WLDNKHTVFG RVTKGMEVVQ RISNVKVNPK TDKPYEDVSI INITVK 

« Hide

Isoform 2 [UniParc].

Checksum: D82E40743B31C263
Show »

FASTA49055,700

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Esophagus.
[2]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone.
[4]"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-646 (ISOFORM 1).
Tissue: Bone marrow.
[5]"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1)."
Davis T.L., Walker J.R., Ouyang H., MacKenzie F., Butler-Cole C., Newman E.M., Eisenmesser E.Z., Dhe-Paganon S.
FEBS J. 275:2283-2295(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 473-646, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK301654 mRNA. Translation: BAG63131.1.
AC008560 Genomic DNA. No translation available.
BC015385 mRNA. Translation: AAH15385.1.
D38552 mRNA. Translation: BAA07555.1.
CCDSCCDS3985.1.
RefSeqNP_001265855.1. NM_001278926.1.
NP_001265856.1. NM_001278927.1.
NP_001265858.1. NM_001278929.1.
NP_056157.1. NM_015342.3.
UniGeneHs.121432.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2A2NX-ray1.65A/B/C473-646[»]
ProteinModelPortalQ96BP3.
SMRQ96BP3. Positions 102-318, 357-392, 482-646.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116971. 2 interactions.
IntActQ96BP3. 4 interactions.
MINTMINT-2813736.
STRING9606.ENSP00000261308.

PTM databases

PhosphoSiteQ96BP3.

Polymorphism databases

DMDM74760739.

Proteomic databases

MaxQBQ96BP3.
PaxDbQ96BP3.
PeptideAtlasQ96BP3.
PRIDEQ96BP3.

Protocols and materials databases

DNASU23398.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261308; ENSP00000261308; ENSG00000113593.
ENST00000538977; ENSP00000444496; ENSG00000113593.
GeneID23398.
KEGGhsa:23398.
UCSCuc003jtv.5. human.

Organism-specific databases

CTD23398.
GeneCardsGC05P064895.
HGNCHGNC:28954. PPWD1.
HPAHPA019353.
HPA019360.
neXtProtNX_Q96BP3.
PharmGKBPA142671149.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000213214.
HOVERGENHBG079869.
InParanoidQ96BP3.
KOK12736.
OMARFYMFTN.
OrthoDBEOG7M98FR.
PhylomeDBQ96BP3.
TreeFamTF105686.

Enzyme and pathway databases

SignaLinkQ96BP3.

Gene expression databases

ArrayExpressQ96BP3.
BgeeQ96BP3.
CleanExHS_PPWD1.
GenevestigatorQ96BP3.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
2.40.100.10. 1 hit.
InterProIPR029000. Cyclophilin-like_dom.
IPR002130. Cyclophilin-type_PPIase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00160. Pro_isomerase. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PRINTSPR00153. CSAPPISMRASE.
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50891. SSF50891. 1 hit.
SSF50978. SSF50978. 3 hits.
PROSITEPS50072. CSA_PPIASE_2. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ96BP3.
GenomeRNAi23398.
NextBio45545.
PROQ96BP3.

Entry information

Entry namePPWD1_HUMAN
AccessionPrimary (citable) accession number: Q96BP3
Secondary accession number(s): B4DWR9, Q15002, Q7KZ89
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM