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Q96BP3

- PPWD1_HUMAN

UniProt

Q96BP3 - PPWD1_HUMAN

Protein

Peptidylprolyl isomerase domain and WD repeat-containing protein 1

Gene

PPWD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

    Catalytic activityi

    Peptidylproline (omega=180) = peptidylproline (omega=0).

    GO - Molecular functioni

    1. peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB
    2. protein folding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Rotamase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    SignaLinkiQ96BP3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidylprolyl isomerase domain and WD repeat-containing protein 1 (EC:5.2.1.8)
    Alternative name(s):
    Spliceosome-associated cyclophilin
    Gene namesi
    Name:PPWD1
    Synonyms:KIAA0073
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:28954. PPWD1.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Associated with spliceosomal complexes.

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671149.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 646645Peptidylprolyl isomerase domain and WD repeat-containing protein 1PRO_0000240306Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ96BP3.
    PaxDbiQ96BP3.
    PeptideAtlasiQ96BP3.
    PRIDEiQ96BP3.

    PTM databases

    PhosphoSiteiQ96BP3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96BP3.
    BgeeiQ96BP3.
    CleanExiHS_PPWD1.
    GenevestigatoriQ96BP3.

    Organism-specific databases

    HPAiHPA019353.
    HPA019360.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex.1 Publication

    Protein-protein interaction databases

    BioGridi116971. 2 interactions.
    IntActiQ96BP3. 4 interactions.
    MINTiMINT-2813736.
    STRINGi9606.ENSP00000261308.

    Structurei

    Secondary structure

    1
    646
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi492 – 4987
    Beta strandi501 – 5077
    Turni509 – 5113
    Helixi513 – 52412
    Turni525 – 5306
    Beta strandi535 – 5373
    Turni538 – 5403
    Beta strandi541 – 5444
    Beta strandi549 – 5524
    Beta strandi574 – 5807
    Beta strandi592 – 5976
    Helixi600 – 6023
    Turni603 – 6053
    Beta strandi608 – 6147
    Helixi616 – 6238
    Turni629 – 6313
    Beta strandi634 – 6363
    Beta strandi639 – 6457

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2A2NX-ray1.65A/B/C473-646[»]
    ProteinModelPortaliQ96BP3.
    SMRiQ96BP3. Positions 102-318, 357-392, 482-646.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ96BP3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati88 – 12639WD 1Add
    BLAST
    Repeati131 – 17040WD 2Add
    BLAST
    Repeati221 – 26040WD 3Add
    BLAST
    Repeati278 – 31942WD 4Add
    BLAST
    Domaini490 – 645156PPIase cyclophilin-typePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi12 – 176Poly-Arg

    Sequence similaritiesi

    Contains 1 PPIase cyclophilin-type domain.PROSITE-ProRule annotation
    Contains 4 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000213214.
    HOVERGENiHBG079869.
    InParanoidiQ96BP3.
    KOiK12736.
    OMAiRFYMFTN.
    OrthoDBiEOG7M98FR.
    PhylomeDBiQ96BP3.
    TreeFamiTF105686.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    2.40.100.10. 1 hit.
    InterProiIPR029000. Cyclophilin-like_dom.
    IPR002130. Cyclophilin-type_PPIase_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00160. Pro_isomerase. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view]
    PRINTSiPR00153. CSAPPISMRASE.
    SMARTiSM00320. WD40. 4 hits.
    [Graphical view]
    SUPFAMiSSF50891. SSF50891. 1 hit.
    SSF50978. SSF50978. 3 hits.
    PROSITEiPS50072. CSA_PPIASE_2. 1 hit.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q96BP3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAESGSDFQ QRRRRRRDPE EPEKTELSER ELAVAVAVSQ ENDEENEERW    50
    VGPLPVEATL AKKRKVLEFE RVYLDNLPSA SMYERSYMHR DVITHVVCTK 100
    TDFIITASHD GHVKFWKKIE EGIEFVKHFR SHLGVIESIA VSSEGALFCS 150
    VGDDKAMKVF DVVNFDMINM LKLGYFPGQC EWIYCPGDAI SSVAASEKST 200
    GKIFIYDGRG DNQPLHIFDK LHTSPLTQIR LNPVYKAVVS SDKSGMIEYW 250
    TGPPHEYKFP KNVNWEYKTD TDLYEFAKCK AYPTSVCFSP DGKKIATIGS 300
    DRKVRIFRFV TGKLMRVFDE SLSMFTELQQ MRQQLPDMEF GRRMAVEREL 350
    EKVDAVRLIN IVFDETGHFV LYGTMLGIKV INVETNRCVR ILGKQENIRV 400
    MQLALFQGIA KKHRAATTIE MKASENPVLQ NIQADPTIVC TSFKKNRFYM 450
    FTKREPEDTK SADSDRDVFN EKPSKEEVMA ATQAEGPKRV SDSAIIHTSM 500
    GDIHTKLFPV ECPKTVENFC VHSRNGYYNG HTFHRIIKGF MIQTGDPTGT 550
    GMGGESIWGG EFEDEFHSTL RHDRPYTLSM ANAGSNTNGS QFFITVVPTP 600
    WLDNKHTVFG RVTKGMEVVQ RISNVKVNPK TDKPYEDVSI INITVK 646
    Length:646
    Mass (Da):73,575
    Last modified:December 1, 2001 - v1
    Checksum:i4DEFA1C78F914430
    GO
    Isoform 2 (identifier: Q96BP3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-156: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:490
    Mass (Da):55,700
    Checksum:iD82E40743B31C263
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 156156Missing in isoform 2. 1 PublicationVSP_055266Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK301654 mRNA. Translation: BAG63131.1.
    AC008560 Genomic DNA. No translation available.
    BC015385 mRNA. Translation: AAH15385.1.
    D38552 mRNA. Translation: BAA07555.1.
    CCDSiCCDS3985.1. [Q96BP3-1]
    CCDS64162.1. [Q96BP3-2]
    RefSeqiNP_001265855.1. NM_001278926.1.
    NP_001265856.1. NM_001278927.1.
    NP_001265858.1. NM_001278929.1.
    NP_056157.1. NM_015342.3.
    UniGeneiHs.121432.

    Genome annotation databases

    EnsembliENST00000261308; ENSP00000261308; ENSG00000113593. [Q96BP3-1]
    ENST00000538977; ENSP00000444496; ENSG00000113593. [Q96BP3-2]
    GeneIDi23398.
    KEGGihsa:23398.
    UCSCiuc003jtv.5. human. [Q96BP3-1]

    Polymorphism databases

    DMDMi74760739.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK301654 mRNA. Translation: BAG63131.1 .
    AC008560 Genomic DNA. No translation available.
    BC015385 mRNA. Translation: AAH15385.1 .
    D38552 mRNA. Translation: BAA07555.1 .
    CCDSi CCDS3985.1. [Q96BP3-1 ]
    CCDS64162.1. [Q96BP3-2 ]
    RefSeqi NP_001265855.1. NM_001278926.1.
    NP_001265856.1. NM_001278927.1.
    NP_001265858.1. NM_001278929.1.
    NP_056157.1. NM_015342.3.
    UniGenei Hs.121432.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2A2N X-ray 1.65 A/B/C 473-646 [» ]
    ProteinModelPortali Q96BP3.
    SMRi Q96BP3. Positions 102-318, 357-392, 482-646.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116971. 2 interactions.
    IntActi Q96BP3. 4 interactions.
    MINTi MINT-2813736.
    STRINGi 9606.ENSP00000261308.

    PTM databases

    PhosphoSitei Q96BP3.

    Polymorphism databases

    DMDMi 74760739.

    Proteomic databases

    MaxQBi Q96BP3.
    PaxDbi Q96BP3.
    PeptideAtlasi Q96BP3.
    PRIDEi Q96BP3.

    Protocols and materials databases

    DNASUi 23398.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261308 ; ENSP00000261308 ; ENSG00000113593 . [Q96BP3-1 ]
    ENST00000538977 ; ENSP00000444496 ; ENSG00000113593 . [Q96BP3-2 ]
    GeneIDi 23398.
    KEGGi hsa:23398.
    UCSCi uc003jtv.5. human. [Q96BP3-1 ]

    Organism-specific databases

    CTDi 23398.
    GeneCardsi GC05P064895.
    HGNCi HGNC:28954. PPWD1.
    HPAi HPA019353.
    HPA019360.
    neXtProti NX_Q96BP3.
    PharmGKBi PA142671149.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000213214.
    HOVERGENi HBG079869.
    InParanoidi Q96BP3.
    KOi K12736.
    OMAi RFYMFTN.
    OrthoDBi EOG7M98FR.
    PhylomeDBi Q96BP3.
    TreeFami TF105686.

    Enzyme and pathway databases

    SignaLinki Q96BP3.

    Miscellaneous databases

    EvolutionaryTracei Q96BP3.
    GenomeRNAii 23398.
    NextBioi 35475797.
    PROi Q96BP3.

    Gene expression databases

    ArrayExpressi Q96BP3.
    Bgeei Q96BP3.
    CleanExi HS_PPWD1.
    Genevestigatori Q96BP3.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    2.40.100.10. 1 hit.
    InterProi IPR029000. Cyclophilin-like_dom.
    IPR002130. Cyclophilin-type_PPIase_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00160. Pro_isomerase. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view ]
    PRINTSi PR00153. CSAPPISMRASE.
    SMARTi SM00320. WD40. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50891. SSF50891. 1 hit.
    SSF50978. SSF50978. 3 hits.
    PROSITEi PS50072. CSA_PPIASE_2. 1 hit.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Esophagus.
    2. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Bone.
    4. "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
      Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
      DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-646 (ISOFORM 1).
      Tissue: Bone marrow.
    5. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1)."
      Davis T.L., Walker J.R., Ouyang H., MacKenzie F., Butler-Cole C., Newman E.M., Eisenmesser E.Z., Dhe-Paganon S.
      FEBS J. 275:2283-2295(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 473-646, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPWD1_HUMAN
    AccessioniPrimary (citable) accession number: Q96BP3
    Secondary accession number(s): B4DWR9, Q15002, Q7KZ89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3