Peptidylprolyl isomerase domain and WD repeat-containing protein 1

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Q96BP3 (PPWD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peptidylprolyl isomerase domain and WD repeat-containing protein 1
EC=5.2.1.8

Alternative name(s):
Spliceosome-associated cyclophilin

Gene names

Name:	PPWD1
Synonyms:	KIAA0073

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Subunit structure	Identified in the spliceosome C complex. Ref.3
Subcellular location	Nucleus. Note: Associated with spliceosomal complexes. Ref.5
Sequence similarities	Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily. Contains 1 PPIase cyclophilin-type domain. Contains 4 WD repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat WD repeat
Molecular function	Isomerase Rotamase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator Ref.3. Source: UniProtKB protein folding Inferred from electronic annotation. Source: UniProtKB-KW protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 646

646

Peptidylprolyl isomerase domain and WD repeat-containing protein 1

PRO_0000240306

Regions

Repeat

88 – 126

WD 1

Repeat

131 – 170

WD 2

Repeat

221 – 260

WD 3

Repeat

278 – 319

WD 4

Domain

490 – 645

156

PPIase cyclophilin-type

Compositional bias

12 – 17

Poly-Arg

Secondary structure

646

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q96BP3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 4DEFA1C78F914430
Show »

FASTA

646

73,575

        10         20         30         40         50         60 
MAAESGSDFQ QRRRRRRDPE EPEKTELSER ELAVAVAVSQ ENDEENEERW VGPLPVEATL 

        70         80         90        100        110        120 
AKKRKVLEFE RVYLDNLPSA SMYERSYMHR DVITHVVCTK TDFIITASHD GHVKFWKKIE 

       130        140        150        160        170        180 
EGIEFVKHFR SHLGVIESIA VSSEGALFCS VGDDKAMKVF DVVNFDMINM LKLGYFPGQC 

       190        200        210        220        230        240 
EWIYCPGDAI SSVAASEKST GKIFIYDGRG DNQPLHIFDK LHTSPLTQIR LNPVYKAVVS 

       250        260        270        280        290        300 
SDKSGMIEYW TGPPHEYKFP KNVNWEYKTD TDLYEFAKCK AYPTSVCFSP DGKKIATIGS 

       310        320        330        340        350        360 
DRKVRIFRFV TGKLMRVFDE SLSMFTELQQ MRQQLPDMEF GRRMAVEREL EKVDAVRLIN 

       370        380        390        400        410        420 
IVFDETGHFV LYGTMLGIKV INVETNRCVR ILGKQENIRV MQLALFQGIA KKHRAATTIE 

       430        440        450        460        470        480 
MKASENPVLQ NIQADPTIVC TSFKKNRFYM FTKREPEDTK SADSDRDVFN EKPSKEEVMA 

       490        500        510        520        530        540 
ATQAEGPKRV SDSAIIHTSM GDIHTKLFPV ECPKTVENFC VHSRNGYYNG HTFHRIIKGF 

       550        560        570        580        590        600 
MIQTGDPTGT GMGGESIWGG EFEDEFHSTL RHDRPYTLSM ANAGSNTNGS QFFITVVPTP 

       610        620        630        640 
WLDNKHTVFG RVTKGMEVVQ RISNVKVNPK TDKPYEDVSI INITVK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone.
[2]	"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1." Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S. DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-646. Tissue: Bone marrow.
[3]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1)." Davis T.L., Walker J.R., Ouyang H., MacKenzie F., Butler-Cole C., Newman E.M., Eisenmesser E.Z., Dhe-Paganon S. FEBS J. 275:2283-2295(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 473-646, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC015385 mRNA. Translation: AAH15385.1.
D38552 mRNA. Translation: BAA07555.1.

IPI

IPI00149650.

RefSeq

NP_056157.1. NM_015342.3.

UniGene

Hs.121432.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A2N	X-ray	1.65	A/B/C	473-646	[»]

ProteinModelPortal

Q96BP3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q96BP3. 4 interactions.

STRING

9606.ENSP00000261308.

PTM databases

PhosphoSite

Q96BP3.

Polymorphism databases

DMDM

74760739.

Proteomic databases

PaxDb

Q96BP3.

PeptideAtlas

Q96BP3.

PRIDE

Q96BP3.

Protocols and materials databases

DNASU

23398.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000261308; ENSP00000261308; ENSG00000113593.

GeneID

23398.

KEGG

hsa:23398.

UCSC

uc003jtv.4. human.

Organism-specific databases

CTD

23398.

GeneCards

GC05P064895.

HGNC

HGNC:28954. PPWD1.

HPA

HPA019353.
HPA019360.

neXtProt

NX_Q96BP3.

PharmGKB

PA142671149.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2319.

HOGENOM

HOG000213214.

HOVERGEN

HBG079869.

InParanoid

Q96BP3.

K12736.

OMA

EDEFHPR.

OrthoDB

EOG4VDPXZ.

PhylomeDB

Q96BP3.

Gene expression databases

ArrayExpress

Q96BP3.

Bgee

Q96BP3.

CleanEx

HS_PPWD1.

Genevestigator

Q96BP3.

GermOnline

ENSG00000113593. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 2 hits.

InterPro

IPR002130. Cyclophilin-like_PPIase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF00160. Pro_isomerase. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]

PRINTS

PR00153. CSAPPISMRASE.

SMART

SM00320. WD40. 4 hits.
[Graphical view]

SUPFAM

SSF50891. CSA_PPIase. 1 hit.
SSF50978. WD40_like. 1 hit.

PROSITE

PS00170. CSA_PPIASE_1. False negative.
PS50072. CSA_PPIASE_2. 1 hit.
PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q96BP3.

GenomeRNAi

23398.

NextBio

45545.

Entry information

Entry name

PPWD1_HUMAN

Accession

Primary (citable) accession number: Q96BP3
Secondary accession number(s): Q15002, Q7KZ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents