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Protein

ADP-ribosylation factor-like protein 8A

Gene

ARL8A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in lysosomes motility. Alternatively, may play a role in chromosome segregation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 357GTP
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi130 – 1334GTP

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 8A
Alternative name(s):
ADP-ribosylation factor-like protein 10B
Novel small G protein indispensable for equal chromosome segregation 2
Gene namesi
Name:ARL8A
Synonyms:ARL10B, GIE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25192. ARL8A.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei1 – 1919Note=Mediates targeting to membranesBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • late endosome membrane Source: UniProtKB-SubCell
  • lysosomal membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • midbody Source: UniProtKB
  • spindle midzone Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134905021.

Polymorphism and mutation databases

BioMutaiARL8A.
DMDMi74751775.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186ADP-ribosylation factor-like protein 8APRO_0000232916Add
BLAST

Proteomic databases

EPDiQ96BM9.
MaxQBiQ96BM9.
PaxDbiQ96BM9.
PeptideAtlasiQ96BM9.
PRIDEiQ96BM9.

PTM databases

iPTMnetiQ96BM9.
PhosphoSiteiQ96BM9.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ96BM9.
CleanExiHS_ARL8A.
ExpressionAtlasiQ96BM9. baseline and differential.
GenevisibleiQ96BM9. HS.

Organism-specific databases

HPAiHPA038759.
HPA040515.
HPA045924.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PUF60Q9UHX13EBI-4401082,EBI-1053259
UQCRHP079193EBI-4401082,EBI-1224427

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi126084. 12 interactions.
IntActiQ96BM9. 4 interactions.
STRINGi9606.ENSP00000272217.

Structurei

Secondary structure

1
186
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 179Combined sources
Beta strandi19 – 268Combined sources
Helixi33 – 4210Combined sources
Beta strandi46 – 494Combined sources
Beta strandi54 – 629Combined sources
Beta strandi65 – 728Combined sources
Helixi76 – 794Combined sources
Helixi82 – 865Combined sources
Beta strandi90 – 978Combined sources
Helixi101 – 1033Combined sources
Helixi104 – 11512Combined sources
Helixi118 – 1203Combined sources
Beta strandi125 – 1306Combined sources
Helixi140 – 1467Combined sources
Helixi149 – 1513Combined sources
Beta strandi157 – 1615Combined sources
Turni164 – 1663Combined sources
Helixi170 – 17910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZD9X-ray1.70A18-186[»]
2H18X-ray1.90A9-182[»]
4ILEX-ray2.68A1-181[»]
ProteinModelPortaliQ96BM9.
SMRiQ96BM9. Positions 18-182.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96BM9.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163692.
HOVERGENiHBG105214.
InParanoidiQ96BM9.
KOiK07955.
OMAiNVMWERY.
OrthoDBiEOG7F24V2.
PhylomeDBiQ96BM9.
TreeFamiTF105470.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96BM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALFNKLLD WFKALFWKEE MELTLVGLQY SGKTTFVNVI ASGQFNEDMI
60 70 80 90 100
PTVGFNMRKI TKGNVTIKLW DIGGQPRFRS MWERYCRGVS AIVYMVDAAD
110 120 130 140 150
QEKIEASKNE LHNLLDKPQL QGIPVLVLGN KRDLPGALDE KELIEKMNLS
160 170 180
AIQDREICCY SISCKEKDNI DITLQWLIQH SKSRRS
Length:186
Mass (Da):21,416
Last modified:December 1, 2001 - v1
Checksum:iEE5141235CE0F414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118752 mRNA. Translation: BAD23993.1.
AK127138 mRNA. Translation: BAG54442.1.
AL592300 Genomic DNA. Translation: CAI13107.1.
CH471067 Genomic DNA. Translation: EAW91395.1.
BC015408 mRNA. Translation: AAH15408.1.
CCDSiCCDS1421.1.
RefSeqiNP_001243058.1. NM_001256129.1.
NP_620150.1. NM_138795.3.
UniGeneiHs.497399.
Hs.604697.

Genome annotation databases

EnsembliENST00000272217; ENSP00000272217; ENSG00000143862.
GeneIDi127829.
KEGGihsa:127829.
UCSCiuc001gxk.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118752 mRNA. Translation: BAD23993.1.
AK127138 mRNA. Translation: BAG54442.1.
AL592300 Genomic DNA. Translation: CAI13107.1.
CH471067 Genomic DNA. Translation: EAW91395.1.
BC015408 mRNA. Translation: AAH15408.1.
CCDSiCCDS1421.1.
RefSeqiNP_001243058.1. NM_001256129.1.
NP_620150.1. NM_138795.3.
UniGeneiHs.497399.
Hs.604697.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZD9X-ray1.70A18-186[»]
2H18X-ray1.90A9-182[»]
4ILEX-ray2.68A1-181[»]
ProteinModelPortaliQ96BM9.
SMRiQ96BM9. Positions 18-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126084. 12 interactions.
IntActiQ96BM9. 4 interactions.
STRINGi9606.ENSP00000272217.

PTM databases

iPTMnetiQ96BM9.
PhosphoSiteiQ96BM9.

Polymorphism and mutation databases

BioMutaiARL8A.
DMDMi74751775.

Proteomic databases

EPDiQ96BM9.
MaxQBiQ96BM9.
PaxDbiQ96BM9.
PeptideAtlasiQ96BM9.
PRIDEiQ96BM9.

Protocols and materials databases

DNASUi127829.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272217; ENSP00000272217; ENSG00000143862.
GeneIDi127829.
KEGGihsa:127829.
UCSCiuc001gxk.3. human.

Organism-specific databases

CTDi127829.
GeneCardsiARL8A.
H-InvDBHIX0200127.
HGNCiHGNC:25192. ARL8A.
HPAiHPA038759.
HPA040515.
HPA045924.
MIMi616597. gene.
neXtProtiNX_Q96BM9.
PharmGKBiPA134905021.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163692.
HOVERGENiHBG105214.
InParanoidiQ96BM9.
KOiK07955.
OMAiNVMWERY.
OrthoDBiEOG7F24V2.
PhylomeDBiQ96BM9.
TreeFamiTF105470.

Miscellaneous databases

ChiTaRSiARL8A. human.
EvolutionaryTraceiQ96BM9.
GeneWikiiARL8A.
GenomeRNAii127829.
PROiQ96BM9.
SOURCEiSearch...

Gene expression databases

BgeeiQ96BM9.
CleanExiHS_ARL8A.
ExpressionAtlasiQ96BM9. baseline and differential.
GenevisibleiQ96BM9. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation."
    Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T.
    J. Cell Sci. 117:4705-4715(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Caudate nucleus.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  6. "An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility."
    Hofmann I., Munro S.
    J. Cell Sci. 119:1494-1503(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Placenta.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Structure of human ADP-ribosylation factor-like 10B."
    Structural genomics consortium (SGC)
    Submitted (MAY-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 18-186 IN COMPLEX WITH GTP ANALOG.

Entry informationi

Entry nameiARL8A_HUMAN
AccessioniPrimary (citable) accession number: Q96BM9
Secondary accession number(s): B3KXD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.