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Protein

Epididymal sperm-binding protein 1

Gene

ELSPBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymal sperm-binding protein 1
Alternative name(s):
Epididymal secretory protein 12
Short name:
hE12
Gene namesi
Name:ELSPBP1
Synonyms:E12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:14417. ELSPBP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27765.

Polymorphism and mutation databases

BioMutaiELSPBP1.
DMDMi296434494.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 223199Epididymal sperm-binding protein 1PRO_0000308249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 100PROSITE-ProRule annotation
Disulfide bondi88 ↔ 115PROSITE-ProRule annotation
Disulfide bondi129 ↔ 153PROSITE-ProRule annotation
Disulfide bondi143 ↔ 168PROSITE-ProRule annotation
Disulfide bondi182 ↔ 208PROSITE-ProRule annotation
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence analysis
Disulfide bondi196 ↔ 223PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ96BH3.
PeptideAtlasiQ96BH3.
PRIDEiQ96BH3.

PTM databases

iPTMnetiQ96BH3.

Expressioni

Tissue specificityi

Detected in cauda epididymidal fluid and on sperm membrane (at protein level).2 Publications

Gene expression databases

BgeeiQ96BH3.
CleanExiHS_ELSPBP1.
ExpressionAtlasiQ96BH3. baseline and differential.
GenevisibleiQ96BH3. HS.

Organism-specific databases

HPAiHPA044256.
HPA050341.

Interactioni

Protein-protein interaction databases

BioGridi122060. 12 interactions.
STRINGi9606.ENSP00000340660.

Structurei

3D structure databases

ProteinModelPortaliQ96BH3.
SMRiQ96BH3. Positions 27-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 6844Fibronectin type-II 1PROSITE-ProRule annotationAdd
BLAST
Domaini69 – 11749Fibronectin type-II 2PROSITE-ProRule annotationAdd
BLAST
Domaini124 – 17047Fibronectin type-II 3PROSITE-ProRule annotationAdd
BLAST
Domaini177 – 22347Fibronectin type-II 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the seminal plasma protein family.Curated
Contains 4 fibronectin type-II domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00830000128254.
HOGENOMiHOG000168399.
HOVERGENiHBG098408.
InParanoidiQ96BH3.
OMAiCETNEYG.
OrthoDBiEOG7FZ005.
PhylomeDBiQ96BH3.
TreeFamiTF343543.

Family and domain databases

Gene3Di2.10.10.10. 4 hits.
InterProiIPR000562. FN_type2_col-bd.
IPR013806. Kringle-like.
[Graphical view]
PfamiPF00040. fn2. 4 hits.
[Graphical view]
SMARTiSM00059. FN2. 4 hits.
[Graphical view]
SUPFAMiSSF57440. SSF57440. 4 hits.
PROSITEiPS00023. FN2_1. 1 hit.
PS51092. FN2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96BH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRWSSYLLG WTTFLLYSYE SSGGMHEECV FPFTYKGSVY FTCTHIHSLS
60 70 80 90 100
PWCATRAVYN GQWKYCQSED YPRCIFPFIY RGKAYNSCIS QGSFLGSLWC
110 120 130 140 150
SVTSVFDEKQ QWKFCETNEY GGNSLRKPCI FPSIYRNNVV SDCMEDESNK
160 170 180 190 200
LWCPTTENMD KDGKWSFCAD TRISALVPGF PCHFPFNYKN KNYFNCTNEG
210 220
SKENLVWCAT SYNYDQDHTW VYC
Length:223
Mass (Da):26,106
Last modified:May 18, 2010 - v2
Checksum:iFC7C215BA3E5CE51
GO

Sequence cautioni

The sequence CAC14267.1 differs from that shown. Reason: Frameshift at position 9. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21T → N in CAC14267 (PubMed:11144225).Curated
Sequence conflicti221 – 2211V → A in AAK69481 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti100 – 1001C → W.
Corresponds to variant rs3745751 [ dbSNP | Ensembl ].
VAR_036760
Natural varianti170 – 1701D → N.
Corresponds to variant rs35362679 [ dbSNP | Ensembl ].
VAR_036761
Natural varianti199 – 1991E → K.3 Publications
Corresponds to variant rs2303690 [ dbSNP | Ensembl ].
VAR_036762
Natural varianti215 – 2151D → N.
Corresponds to variant rs6509358 [ dbSNP | Ensembl ].
VAR_036763

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278478 mRNA. Translation: CAC14267.1. Frameshift.
AF279147 mRNA. Translation: AAK69481.1.
AC010330 Genomic DNA. No translation available.
BC015598 mRNA. Translation: AAH15598.1.
CCDSiCCDS12708.1.
RefSeqiNP_071425.3. NM_022142.4.
UniGeneiHs.104894.

Genome annotation databases

EnsembliENST00000339841; ENSP00000340660; ENSG00000169393.
GeneIDi64100.
KEGGihsa:64100.
UCSCiuc002pht.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278478 mRNA. Translation: CAC14267.1. Frameshift.
AF279147 mRNA. Translation: AAK69481.1.
AC010330 Genomic DNA. No translation available.
BC015598 mRNA. Translation: AAH15598.1.
CCDSiCCDS12708.1.
RefSeqiNP_071425.3. NM_022142.4.
UniGeneiHs.104894.

3D structure databases

ProteinModelPortaliQ96BH3.
SMRiQ96BH3. Positions 27-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122060. 12 interactions.
STRINGi9606.ENSP00000340660.

PTM databases

iPTMnetiQ96BH3.

Polymorphism and mutation databases

BioMutaiELSPBP1.
DMDMi296434494.

Proteomic databases

PaxDbiQ96BH3.
PeptideAtlasiQ96BH3.
PRIDEiQ96BH3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339841; ENSP00000340660; ENSG00000169393.
GeneIDi64100.
KEGGihsa:64100.
UCSCiuc002pht.4. human.

Organism-specific databases

CTDi64100.
GeneCardsiELSPBP1.
H-InvDBHIX0015284.
HGNCiHGNC:14417. ELSPBP1.
HPAiHPA044256.
HPA050341.
MIMi607443. gene.
neXtProtiNX_Q96BH3.
PharmGKBiPA27765.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00830000128254.
HOGENOMiHOG000168399.
HOVERGENiHBG098408.
InParanoidiQ96BH3.
OMAiCETNEYG.
OrthoDBiEOG7FZ005.
PhylomeDBiQ96BH3.
TreeFamiTF343543.

Miscellaneous databases

GenomeRNAii64100.
PROiQ96BH3.
SOURCEiSearch...

Gene expression databases

BgeeiQ96BH3.
CleanExiHS_ELSPBP1.
ExpressionAtlasiQ96BH3. baseline and differential.
GenevisibleiQ96BH3. HS.

Family and domain databases

Gene3Di2.10.10.10. 4 hits.
InterProiIPR000562. FN_type2_col-bd.
IPR013806. Kringle-like.
[Graphical view]
PfamiPF00040. fn2. 4 hits.
[Graphical view]
SMARTiSM00059. FN2. 4 hits.
[Graphical view]
SUPFAMiSSF57440. SSF57440. 4 hits.
PROSITEiPS00023. FN2_1. 1 hit.
PS51092. FN2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel sperm-binding proteins of epididymal origin contain four fibronectin type II-modules."
    Saalmann A., Muenz S., Ellerbrock K., Ivell R., Kirchhoff C.
    Mol. Reprod. Dev. 58:88-100(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT LYS-199.
  2. "A new human homolog 1 of bovine seminal plasma proteins."
    Luo Y., Zhang X., Yu B.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-199.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-199.
    Tissue: Placenta.
  5. "Sperm-binding fibronectin type II-module proteins are genetically linked and functionally related."
    Ekhlasi-Hundrieser M., Schaefer B., Philipp U., Kuiper H., Leeb T., Mehta M., Kirchhoff C., Toepfer-Petersen E.
    Gene 392:253-265(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiESPB1_HUMAN
AccessioniPrimary (citable) accession number: Q96BH3
Secondary accession number(s): Q96RT0, Q9H4C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: May 18, 2010
Last modified: July 6, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.