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Protein

SPRY domain-containing SOCS box protein 1

Gene

SPSB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171621-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 1
Short name:
SSB-1
Gene namesi
Name:SPSB1
Synonyms:SSB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:30628. SPSB1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31Y → F: Loss of phosphorylation. 1 Publication1
Mutagenesisi260 – 263LPLP → AAAA: Abolishes interaction with RNF7 and CUL5. 1 Publication4

Organism-specific databases

DisGeNETi80176.
OpenTargetsiENSG00000171621.
PharmGKBiPA142670871.

Polymorphism and mutation databases

BioMutaiSPSB1.
DMDMi74731225.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384721 – 273SPRY domain-containing SOCS box protein 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Phosphotyrosine; by MET1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96BD6.
PRIDEiQ96BD6.

PTM databases

iPTMnetiQ96BD6.
PhosphoSitePlusiQ96BD6.

Expressioni

Gene expression databases

BgeeiENSG00000171621.
CleanExiHS_SPSB1.
ExpressionAtlasiQ96BD6. baseline and differential.
GenevisibleiQ96BD6. HS.

Organism-specific databases

HPAiHPA003296.

Interactioni

Subunit structurei

Component of the probable ECS(SPSB1) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB1. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Directly interacts with MET tyrosine kinase domain in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. When phosphorylated, interacts with RASA1 without affecting its stability. Interacts, in complex with Elongin BC complex, with PAWR.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KDM1AO603412EBI-2659201,EBI-710124
PAWRQ96IZ02EBI-2659201,EBI-595869
PRMT1Q998732EBI-2659201,EBI-78738
PRMT6Q96LA82EBI-2659201,EBI-912440
SNAI1O958633EBI-2659201,EBI-1045459
SUV39H1O434632EBI-2659201,EBI-349968
vasP090522EBI-2659201,EBI-134067From a different organism.

Protein-protein interaction databases

BioGridi123158. 22 interactors.
IntActiQ96BD6. 12 interactors.
MINTiMINT-6780046.
STRINGi9606.ENSP00000330221.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 42Combined sources7
Helixi48 – 53Combined sources6
Beta strandi55 – 61Combined sources7
Beta strandi65 – 68Combined sources4
Beta strandi71 – 77Combined sources7
Beta strandi83 – 90Combined sources8
Beta strandi95 – 103Combined sources9
Helixi106 – 108Combined sources3
Beta strandi114 – 118Combined sources5
Beta strandi125 – 129Combined sources5
Beta strandi139 – 143Combined sources5
Turni144 – 147Combined sources4
Beta strandi148 – 152Combined sources5
Turni153 – 155Combined sources3
Beta strandi159 – 162Combined sources4
Beta strandi175 – 182Combined sources8
Turni183 – 186Combined sources4
Beta strandi187 – 192Combined sources6
Beta strandi195 – 201Combined sources7
Beta strandi209 – 215Combined sources7
Beta strandi221 – 230Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JK9X-ray1.79A24-233[»]
3F2OX-ray2.05A/B24-233[»]
ProteinModelPortaliQ96BD6.
SMRiQ96BD6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96BD6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 231B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199
Domaini232 – 273SOCS boxPROSITE-ProRule annotationAdd BLAST42

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). The B30.2/SPRY domain is involved in MET binding.By similarity

Sequence similaritiesi

Belongs to the SPSB family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiQ96BD6.
KOiK10343.
OMAiGMESECP.
OrthoDBiEOG091G0DF0.
PhylomeDBiQ96BD6.
TreeFamiTF312822.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96BD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV
60 70 80 90 100
QLLHSWNNND RSLNVFVKED DKLIFHRHPV AQSTDAIRGK VGYTRGLHVW
110 120 130 140 150
QITWAMRQRG THAVVGVATA DAPLHSVGYT TLVGNNHESW GWDLGRNRLY
160 170 180 190 200
HDGKNQPSKT YPAFLEPDET FIVPDSFLVA LDMDDGTLSF IVDGQYMGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EIRMRYLNGL DPEPLPLMDL CRRSVRLALG
260 270
RERLGEIHTL PLPASLKAYL LYQ
Length:273
Mass (Da):30,942
Last modified:December 1, 2001 - v1
Checksum:iF93D5690A32403DF
GO

Sequence cautioni

The sequence BAD92796 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14M → L in BAD92796 (Ref. 3) Curated1
Sequence conflicti73L → P in BAB15335 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403026 mRNA. Translation: AAL57345.1.
AK026046 mRNA. Translation: BAB15335.1.
AB209559 mRNA. Translation: BAD92796.1. Different initiation.
AL358252, AL008734 Genomic DNA. Translation: CAI14376.2.
AL008734, AL358252 Genomic DNA. Translation: CAM12843.1.
CH471130 Genomic DNA. Translation: EAW71617.1.
BC005852 mRNA. Translation: AAH05852.2.
BC015711 mRNA. Translation: AAH15711.1.
CCDSiCCDS102.1.
RefSeqiNP_079382.2. NM_025106.3.
UniGeneiHs.738680.
Hs.8261.

Genome annotation databases

EnsembliENST00000328089; ENSP00000330221; ENSG00000171621.
ENST00000357898; ENSP00000350573; ENSG00000171621.
ENST00000377399; ENSP00000366616; ENSG00000171621.
GeneIDi80176.
KEGGihsa:80176.
UCSCiuc001apv.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403026 mRNA. Translation: AAL57345.1.
AK026046 mRNA. Translation: BAB15335.1.
AB209559 mRNA. Translation: BAD92796.1. Different initiation.
AL358252, AL008734 Genomic DNA. Translation: CAI14376.2.
AL008734, AL358252 Genomic DNA. Translation: CAM12843.1.
CH471130 Genomic DNA. Translation: EAW71617.1.
BC005852 mRNA. Translation: AAH05852.2.
BC015711 mRNA. Translation: AAH15711.1.
CCDSiCCDS102.1.
RefSeqiNP_079382.2. NM_025106.3.
UniGeneiHs.738680.
Hs.8261.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JK9X-ray1.79A24-233[»]
3F2OX-ray2.05A/B24-233[»]
ProteinModelPortaliQ96BD6.
SMRiQ96BD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123158. 22 interactors.
IntActiQ96BD6. 12 interactors.
MINTiMINT-6780046.
STRINGi9606.ENSP00000330221.

PTM databases

iPTMnetiQ96BD6.
PhosphoSitePlusiQ96BD6.

Polymorphism and mutation databases

BioMutaiSPSB1.
DMDMi74731225.

Proteomic databases

PaxDbiQ96BD6.
PRIDEiQ96BD6.

Protocols and materials databases

DNASUi80176.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328089; ENSP00000330221; ENSG00000171621.
ENST00000357898; ENSP00000350573; ENSG00000171621.
ENST00000377399; ENSP00000366616; ENSG00000171621.
GeneIDi80176.
KEGGihsa:80176.
UCSCiuc001apv.4. human.

Organism-specific databases

CTDi80176.
DisGeNETi80176.
GeneCardsiSPSB1.
HGNCiHGNC:30628. SPSB1.
HPAiHPA003296.
MIMi611657. gene.
neXtProtiNX_Q96BD6.
OpenTargetsiENSG00000171621.
PharmGKBiPA142670871.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiQ96BD6.
KOiK10343.
OMAiGMESECP.
OrthoDBiEOG091G0DF0.
PhylomeDBiQ96BD6.
TreeFamiTF312822.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000171621-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiSPSB1. human.
EvolutionaryTraceiQ96BD6.
GeneWikiiSPSB1.
GenomeRNAii80176.
PROiQ96BD6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171621.
CleanExiHS_SPSB1.
ExpressionAtlasiQ96BD6. baseline and differential.
GenevisibleiQ96BD6. HS.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPSB1_HUMAN
AccessioniPrimary (citable) accession number: Q96BD6
Secondary accession number(s): A2A275
, Q59FA1, Q5TIH9, Q9BRY9, Q9H6C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.