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Protein

SPRY domain-containing SOCS box protein 1

Gene

SPSB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 1
Short name:
SSB-1
Gene namesi
Name:SPSB1
Synonyms:SSB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000171621.13
HGNCiHGNC:30628 SPSB1
MIMi611657 gene
neXtProtiNX_Q96BD6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31Y → F: Loss of phosphorylation. 1 Publication1
Mutagenesisi260 – 263LPLP → AAAA: Abolishes interaction with RNF7 and CUL5. 1 Publication4

Organism-specific databases

DisGeNETi80176
OpenTargetsiENSG00000171621
PharmGKBiPA142670871

Polymorphism and mutation databases

BioMutaiSPSB1
DMDMi74731225

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384721 – 273SPRY domain-containing SOCS box protein 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Phosphotyrosine; by MET1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ96BD6
PeptideAtlasiQ96BD6
PRIDEiQ96BD6

PTM databases

iPTMnetiQ96BD6
PhosphoSitePlusiQ96BD6

Expressioni

Gene expression databases

BgeeiENSG00000171621
CleanExiHS_SPSB1
ExpressionAtlasiQ96BD6 baseline and differential
GenevisibleiQ96BD6 HS

Interactioni

Subunit structurei

Component of the probable ECS(SPSB1) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB1 (PubMed:15601820). Interacts with CUL5, RNF7, ELOB and ELOC (PubMed:15601820). Directly interacts with MET tyrosine kinase domain in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction (PubMed:15713673). When phosphorylated, interacts with RASA1 without affecting its stability (PubMed:15713673). Interacts (via B30.2/SPRY domain) with PAWR; this interaction is direct and occurs in association with the Elongin BC complex (PubMed:17189197, PubMed:20561531).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi123158, 22 interactors
ELMiQ96BD6
IntActiQ96BD6, 12 interactors
MINTiQ96BD6
STRINGi9606.ENSP00000330221

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 42Combined sources7
Helixi48 – 53Combined sources6
Beta strandi55 – 61Combined sources7
Beta strandi65 – 68Combined sources4
Beta strandi71 – 77Combined sources7
Beta strandi83 – 90Combined sources8
Beta strandi95 – 103Combined sources9
Helixi106 – 108Combined sources3
Beta strandi114 – 118Combined sources5
Beta strandi125 – 129Combined sources5
Beta strandi139 – 143Combined sources5
Turni144 – 147Combined sources4
Beta strandi148 – 152Combined sources5
Turni153 – 155Combined sources3
Beta strandi159 – 162Combined sources4
Beta strandi175 – 182Combined sources8
Turni183 – 186Combined sources4
Beta strandi187 – 192Combined sources6
Beta strandi195 – 201Combined sources7
Beta strandi209 – 215Combined sources7
Beta strandi221 – 230Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JK9X-ray1.79A30-231[»]
3F2OX-ray2.05A/B31-233[»]
ProteinModelPortaliQ96BD6
SMRiQ96BD6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96BD6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 231B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199
Domaini232 – 273SOCS boxPROSITE-ProRule annotationAdd BLAST42

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). The B30.2/SPRY domain is involved in MET and PAWR binding (PubMed:15713673, PubMed:20561531).By similarity2 Publications

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiQ96BD6
KOiK10343
OMAiMGQKVPG
OrthoDBiEOG091G0DF0
PhylomeDBiQ96BD6
TreeFamiTF312822

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR003877 SPRY_dom
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit

Sequencei

Sequence statusi: Complete.

Q96BD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV
60 70 80 90 100
QLLHSWNNND RSLNVFVKED DKLIFHRHPV AQSTDAIRGK VGYTRGLHVW
110 120 130 140 150
QITWAMRQRG THAVVGVATA DAPLHSVGYT TLVGNNHESW GWDLGRNRLY
160 170 180 190 200
HDGKNQPSKT YPAFLEPDET FIVPDSFLVA LDMDDGTLSF IVDGQYMGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EIRMRYLNGL DPEPLPLMDL CRRSVRLALG
260 270
RERLGEIHTL PLPASLKAYL LYQ
Length:273
Mass (Da):30,942
Last modified:December 1, 2001 - v1
Checksum:iF93D5690A32403DF
GO

Sequence cautioni

The sequence BAD92796 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14M → L in BAD92796 (Ref. 3) Curated1
Sequence conflicti73L → P in BAB15335 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403026 mRNA Translation: AAL57345.1
AK026046 mRNA Translation: BAB15335.1
AB209559 mRNA Translation: BAD92796.1 Different initiation.
AL358252 Genomic DNA No translation available.
AL008734 Genomic DNA No translation available.
CH471130 Genomic DNA Translation: EAW71617.1
BC005852 mRNA Translation: AAH05852.2
BC015711 mRNA Translation: AAH15711.1
CCDSiCCDS102.1
RefSeqiNP_079382.2, NM_025106.3
UniGeneiHs.738680
Hs.8261

Genome annotation databases

EnsembliENST00000328089; ENSP00000330221; ENSG00000171621
ENST00000357898; ENSP00000350573; ENSG00000171621
ENST00000377399; ENSP00000366616; ENSG00000171621
GeneIDi80176
KEGGihsa:80176
UCSCiuc001apv.4 human

Similar proteinsi

Entry informationi

Entry nameiSPSB1_HUMAN
AccessioniPrimary (citable) accession number: Q96BD6
Secondary accession number(s): A2A275
, Q59FA1, Q5TIH9, Q9BRY9, Q9H6C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health