Q96BA8 (CR3L1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclic AMP-responsive element-binding protein 3-like protein 1
Short name=cAMP-responsive element-binding protein 3-like protein 1

Alternative name(s):
Old astrocyte specifically-induced substance
Short name=OASIS

Cleaved into the following chain:

1. Processed cyclic AMP-responsive element-binding protein 3-like protein 1

Gene names

Name:	CREB3L1
Synonyms:	OASIS
ORF Names:	PSEC0238

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE By similarity. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.1 Ref.4. Processed cyclic AMP-responsive element-binding protein 3-like protein 1: Nucleus. Note: Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. Ref.1 Ref.4
Tissue specificity	Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain. Ref.1
Post-translational modification	Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
Sequence similarities	Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence BAC11681.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Unfolded protein response
Cellular component	Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	DNA-binding
Molecular function	Activator
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of bone mineralization Inferred from electronic annotation. Source: Compara response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from electronic annotation. Source: Compara chromatin binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96BA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96BA8-2)

Also known as: OASISv1;

The sequence of this isoform differs from the canonical sequence as follows:
     111-198: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Cyclic AMP-responsive element-binding protein 3-like protein 1		PRO_0000288064
	Chain	1 – ?		Processed cyclic AMP-responsive element-binding protein 3-like protein 1		PRO_0000296206
Regions
	Topological domain	1 – 374	374	Cytoplasmic Potential
	Transmembrane	375 – 395	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	396 – 519	124	Lumenal Potential
	Domain	290 – 353	64	bZIP
	Region	1 – 60	60	Required for transcriptional activation
	Region	292 – 321	30	Basic motif By similarity
	Region	332 – 353	22	Leucine-zipper By similarity
	Compositional bias	146 – 154	9	Poly-Ala
Sites
	Site	426 – 427	2	Cleavage; by PS1 By similarity
Amino acid modifications
	Glycosylation	492	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	111 – 198	88	Missing in isoform 2.		VSP_025632
	Natural variant	411	1	A → T. Corresponds to variant rs35652107 [ dbSNP | Ensembl ].		VAR_032392
Experimental info
	Mutagenesis	392	1	P → L: Abolishes proteolytic cleavage by PS2. Ref.4
	Mutagenesis	423	1	R → A: Abolishes proteolytic cleavage by PS1. Ref.4
	Mutagenesis	426	1	L → V: Abolishes proteolytic cleavage by PS1. Ref.4
	Sequence conflict	249	1	A → P in AAH14097. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D08133CD8B02A3AC	FASTA	519	57,005
	10 20 30 40 50 60 MDAVLEPFPA DRLFPGSSFL DLGDLNESDF LNNAHFPEHL DHFTENMEDF SNDLFSSFFD 70 80 90 100 110 120 DPVLDEKSPL LDMELDSPTP GIQAEHSYSL SGDSAPQSPL VPIKMEDTTQ DAEHGAWALG 130 140 150 160 170 180 HKLCSIMVKQ EQSPELPVDP LAAPSAMAAA AAMATTPLLG LSPLSRLPIP HQAPGEMTQL 190 200 210 220 230 240 PVIKAEPLEV NQFLKVTPED LVQMPPTPPS SHGSDSDGSQ SPRSLPPSSP VRPMARSSTA 250 260 270 280 290 300 ISTSPLLTAP HKLQGTSGPL LLTEEEKRTL IAEGYPIPTK LPLTKAEEKA LKRVRRKIKN 310 320 330 340 350 360 KISAQESRRK KKEYVECLEK KVETFTSENN ELWKKVETLE NANRTLLQQL QKLQTLVTNK 370 380 390 400 410 420 ISRPYKMAAT QTGTCLMVAA LCFVLVLGSL VPCLPEFSSG SQTVKEDPLA ADGVYTASQM 430 440 450 460 470 480 PSRSLLFYDD GAGLWEDGRS TLLPMEPPDG WEINPGGPAE QRPRDHLQHD HLDSTHETTK 490 500 510 YLSEAWPKDG GNGTSPDFSH SKEWFHDRDL GPNTTIKLS
	Isoform 2 (OASISv1) [UniParc]. Checksum: 7DD049831DE6024C	FASTA	431	47,783
	10 20 30 40 50 60 MDAVLEPFPA DRLFPGSSFL DLGDLNESDF LNNAHFPEHL DHFTENMEDF SNDLFSSFFD 70 80 90 100 110 120 DPVLDEKSPL LDMELDSPTP GIQAEHSYSL SGDSAPQSPL VPIKMEDTTQ EDLVQMPPTP 130 140 150 160 170 180 PSSHGSDSDG SQSPRSLPPS SPVRPMARSS TAISTSPLLT APHKLQGTSG PLLLTEEEKR 190 200 210 220 230 240 TLIAEGYPIP TKLPLTKAEE KALKRVRRKI KNKISAQESR RKKKEYVECL EKKVETFTSE 250 260 270 280 290 300 NNELWKKVET LENANRTLLQ QLQKLQTLVT NKISRPYKMA ATQTGTCLMV AALCFVLVLG 310 320 330 340 350 360 SLVPCLPEFS SGSQTVKEDP LAADGVYTAS QMPSRSLLFY DDGAGLWEDG RSTLLPMEPP 370 380 390 400 410 420 DGWEINPGGP AEQRPRDHLQ HDHLDSTHET TKYLSEAWPK DGGNGTSPDF SHSKEWFHDR 430 DLGPNTTIKL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"OASIS is a transcriptional activator of CREB/ATF family with a transmembrane domain." Omori Y., Imai J., Suzuki Y., Watanabe S., Tanigami A., Sugano S. Biochem. Biophys. Res. Commun. 293:470-477(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ALTERNATIVE SPLICING (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 253-519. Tissue: Embryo.
[4]	"Cleavage of the membrane-bound transcription factor OASIS in response to endoplasmic reticulum stress." Murakami T., Kondo S., Ogata M., Kanemoto S., Saito A., Wanaka A., Imaizumi K. J. Neurochem. 96:1090-1100(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-392; ARG-423 AND LEU-426, PROTEOLYTIC PROCESSING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB063321 mRNA. Translation: BAC01278.1. BC014097 mRNA. Translation: AAH14097.1. BC015781 mRNA. Translation: AAH15781.1. AK075538 mRNA. Translation: BAC11681.1. Different initiation.
IPI	IPI00303434. IPI00848028.
RefSeq	NP_443086.1. NM_052854.2.
UniGene	Hs.405961.
3D structure databases
HSSP	HSSP built from PDB template 1T2K based on UniProtKB P15336.
ProteinModelPortal	Q96BA8.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-268046.
STRING	9606.ENSP00000288400.
PTM databases
PhosphoSite	Q96BA8.
Polymorphism databases
DMDM	74751763.
Proteomic databases
PaxDb	Q96BA8.
PRIDE	Q96BA8.
Protocols and materials databases
DNASU	90993.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000288400; ENSP00000288400; ENSG00000157613. ENST00000529193; ENSP00000434939; ENSG00000157613.
GeneID	90993.
KEGG	hsa:90993.
UCSC	uc021qik.1. human.
Organism-specific databases
CTD	90993.
GeneCards	GC11P046299.
HGNC	HGNC:18856. CREB3L1.
HPA	CAB026151. HPA024069.
neXtProt	NX_Q96BA8.
Orphanet	79105. Myxofibrosarcoma.
PharmGKB	PA134960108.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG294764.
HOGENOM	HOG000060150.
HOVERGEN	HBG057480.
InParanoid	Q96BA8.
KO	K09048.
OMA	HGAWALG.
Gene expression databases
ArrayExpress	Q96BA8.
Bgee	Q96BA8.
CleanEx	HS_CREB3L1.
Genevestigator	Q96BA8.
Family and domain databases
InterPro	IPR004827. bZIP. IPR008917. Euk_TF_DNA-bd. IPR001630. Leuzip_CREB. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
PRINTS	PR00041. LEUZIPPRCREB.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
SUPFAM	SSF47454. Euk_transcr_DNA. 1 hit.
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CREB3L1. human.
GenomeRNAi	90993.
NextBio	77064.

Entry information

Entry name

CR3L1_HUMAN

Accession

Primary (citable) accession number: Q96BA8
Secondary accession number(s): Q8N2D5, Q96CP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.