Reviewed,
UniProtKB/Swiss-Prot Q96BA8 (CR3L1_HUMAN)
Last modified
November 25, 2008.
Version 51.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: cAMP-responsive element-binding protein 3-like protein 1 Alternative name(s): OASIS Cleaved into the following chain: 1- Recommended name: Processed cAMP-responsive element-binding protein 3-like protein 1 | ||||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 519 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Processed cAMP-responsive element-binding protein 3-like protein 1: Nucleus. Note= Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. |
| Tissue specificity | Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain. |
| Post-translational modification | Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus. |
| Sequence similarities | Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q96BA8-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q96BA8-2) Also known as: OASISv1; The sequence of this isoform differs from the canonical sequence as follows: 111-198: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 519 | 519 | cAMP-responsive element-binding protein 3-like protein 1 | PRO_0000288064 | |||||
| Chain | 1 – ? | Processed cAMP-responsive element-binding protein 3-like protein 1 | PRO_0000296206 | ||||||
Regions | |||||||||
| Topological domain | 1 – 374 | 374 | Cytoplasmic Potential | ||||||
| Transmembrane | 375 – 395 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 396 – 519 | 124 | Lumenal Potential | ||||||
| Domain | 318 – 353 | 36 | Leucine-zipper | ||||||
| DNA binding | 292 – 312 | 21 | Basic motif | ||||||
| Region | 1 – 60 | 60 | Required for transcriptional activation | ||||||
| Compositional bias | 146 – 154 | 9 | Poly-Ala | ||||||
Sites | |||||||||
| Site | 426 – 427 | 2 | Cleavage; by PS1 By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 492 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 513 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 111 – 198 | 88 | Missing in isoform 2. | VSP_025632 | |||||
| Natural variant | 411 | 1 | A → T: dbSNP rs35652107. | VAR_032392 | |||||
Experimental info | |||||||||
| Mutagenesis | 392 | 1 | P → L: Abolishes proteolytic cleavage by PS2 | ||||||
| Mutagenesis | 423 | 1 | R → A: Abolishes proteolytic cleavage by PS1 | ||||||
| Mutagenesis | 426 | 1 | L → V: Abolishes proteolytic cleavage by PS1 | ||||||
| Sequence conflict | 249 | 1 | A → P in AAH14097. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "OASIS is a transcriptional activator of CREB/ATF family with a transmembrane domain." Omori Y., Imai J., Suzuki Y., Watanabe S., Tanigami A., Sugano S. Biochem. Biophys. Res. Commun. 293:470-477(2002) [PubMed: 12054625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ALTERNATIVE SPLICING (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon. |
| [3] | "HRI human cDNA sequencing project." Ota T., Nishikawa T., Suzuki Y., Kawai-Hio Y., Hayashi K., Ishii S., Saito K., Yamamoto J., Wakamatsu A., Nagai T., Nakamura Y., Nagahari K., Sugano S., Isogai T. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 253-519. Tissue: Embryo. |
| [4] | "Cleavage of the membrane-bound transcription factor OASIS in response to endoplasmic reticulum stress." Murakami T., Kondo S., Ogata M., Kanemoto S., Saito A., Wanaka A., Imaizumi K. J. Neurochem. 96:1090-1100(2006) [PubMed: 16417584] [Abstract] Cited for: SUBCELLULLAR LOCATION, MUTAGENESIS OF PRO-392; ARG-423 AND LEU-426, PROTEOLYTIC PROCESSING. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB063321 mRNA. Translation: BAC01278.1. BC014097 mRNA. Translation: AAH14097.1. BC015781 mRNA. Translation: AAH15781.1. AK075538 mRNA. Translation: BAC11681.1. Different initiation. | |
| RefSeq | NP_443086.1. |
| UniGene | Hs.405961 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NWQ based on UniProtKB P05554. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSG00000157613. Homo sapiens. [Contig view] |
| GeneID | 90993. |
| KEGG | hsa:90993. |
Organism-specific databases | |
| HGNC | HGNC:18856. CREB3L1. |
| PharmGKB | PA134960108. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | Q96BA8. |
| HOVERGEN | Q96BA8. |
Gene expression databases | |
| ArrayExpress | Q96BA8. |
| CleanEx | HS_CREB3L1. |
Family and domain databases | |
| InterPro | IPR011616. bZIP_1. IPR001630. Leuzip_CREB. IPR004827. TF_bZIP. [Graphical view] |
| Pfam | PF00170. bZIP_1. 1 hit. [Graphical view] |
| PRINTS | PR00041. LEUZIPPRCREB. |
| SMART | SM00338. BRLZ. 1 hit. [Graphical view] |
| PROSITE | PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 77064. |
Entry information
| Entry name | CR3L1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q96BA8 Secondary accession number(s): Q8N2D5, Q96CP0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| SIMILARITY comments Index of protein domains and families |

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