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Q96B86 (RGMA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Repulsive guidance molecule A
Alternative name(s):
RGM domain family member A
Gene names
Name:RGMA
Synonyms:RGM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. Ref.7 Ref.8

Subunit structure

Interacts with NEO1, BMP2 and BMP4 By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity.

Sequence similarities

Belongs to the repulsive guidance molecule (RGM) family.

Sequence caution

The sequence AAH15886.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAB98207.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   PTMGlycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Traceable author statement. Source: Reactome

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum

Inferred from direct assay. Source: LIFEdb

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96B86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96B86-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: Missing.
Isoform 3 (identifier: Q96B86-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MQPP → MGGLGPRRAGTS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4747 Potential
Propeptide48 – 168121Removed in mature form By similarity
PRO_0000030385
Chain169 – 424256Repulsive guidance molecule A
PRO_0000030386
Propeptide425 – 45026Removed in mature form Potential
PRO_0000030387

Amino acid modifications

Lipidation4241GPI-anchor amidated alanine Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1591N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1616Missing in isoform 2.
VSP_054070
Alternative sequence1 – 44MQPP → MGGLGPRRAGTS in isoform 3.
VSP_054071
Natural variant4151D → E. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs4238485 [ dbSNP | Ensembl ].
VAR_062144
Natural variant4311A → V.
Corresponds to variant rs4778078 [ dbSNP | Ensembl ].
VAR_060105

Experimental info

Sequence conflict1991V → A in BAC11285. Ref.3
Sequence conflict3141L → P in BAH13014. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: E3244FA43A029DCF

FASTA45049,347
        10         20         30         40         50         60 
MQPPRERLVV TGRAGWMGMG RGAGRSALGF WPTLAFLLCS FPAATSPCKI LKCNSEFWSA 

        70         80         90        100        110        120 
TSGSHAPASD DTPEFCAALR SYALCTRRTA RTCRGDLAYH SAVHGIEDLM SQHNCSKDGP 

       130        140        150        160        170        180 
TSQPRLRTLP PAGDSQERSD SPEICHYEKS FHKHSATPNY THCGLFGDPH LRTFTDRFQT 

       190        200        210        220        230        240 
CKVQGAWPLI DNNYLNVQVT NTPVLPGSAA TATSKLTIIF KNFQECVDQK VYQAEMDELP 

       250        260        270        280        290        300 
AAFVDGSKNG GDKHGANSLK ITEKVSGQHV EIQAKYIGTT IVVRQVGRYL TFAVRMPEEV 

       310        320        330        340        350        360 
VNAVEDWDSQ GLYLCLRGCP LNQQIDFQAF HTNAEGTGAR RLAAASPAPT APETFPYETA 

       370        380        390        400        410        420 
VAKCKEKLPV EDLYYQACVF DLLTTGDVNF TLAAYYALED VKMLHSNKDK LHLYDRTRDL 

       430        440        450 
PGRAAAGLPL APRPLLGALV PLLALLPVFC 

« Hide

Isoform 2 [UniParc].

Checksum: 219D630DAEF61745
Show »

FASTA43447,512
Isoform 3 [UniParc].

Checksum: E01D353C68437163
Show »

FASTA45850,035

References

« Hide 'large scale' references
[1]The European IMAGE consortium
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLU-415.
Tissue: Retina.
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLU-415.
Tissue: Testis.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT GLU-415.
Tissue: Teratocarcinoma.
[4]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-415.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT GLU-415.
Tissue: Brain and Uterus.
[7]"Unc5B associates with LARG to mediate the action of repulsive guidance molecule."
Hata K., Kaibuchi K., Inagaki S., Yamashita T.
J. Cell Biol. 184:737-750(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Inactivation of Ras by p120GAP via focal adhesion kinase dephosphorylation mediates RGMa-induced growth cone collapse."
Endo M., Yamashita T.
J. Neurosci. 29:6649-6662(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL390083 mRNA. Translation: CAB98207.1. Different initiation.
AL136826 mRNA. Translation: CAB66760.1.
AK074910 mRNA. Translation: BAC11285.1.
AK074966 mRNA. Translation: BAC11321.1.
AK074980 mRNA. Translation: BAC11330.1.
AK299363 mRNA. Translation: BAH13014.1.
AC087641 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX02167.1.
BC015886 mRNA. Translation: AAH15886.2. Different initiation.
BC140838 mRNA. Translation: AAI40839.1.
BC151132 mRNA. Translation: AAI51133.1.
CCDSCCDS45357.1. [Q96B86-1]
RefSeqNP_001159755.1. NM_001166283.1.
NP_001159758.1. NM_001166286.1.
NP_001159759.1. NM_001166287.1.
NP_001159760.1. NM_001166288.1.
NP_001159761.1. NM_001166289.1.
NP_064596.2. NM_020211.2.
UniGeneHs.271277.

3D structure databases

ProteinModelPortalQ96B86.
SMRQ96B86. Positions 169-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121284. 3 interactions.
IntActQ96B86. 2 interactions.
MINTMINT-1420050.
STRING9606.ENSP00000330005.

PTM databases

PhosphoSiteQ96B86.

Polymorphism databases

DMDM296452979.

Proteomic databases

PaxDbQ96B86.
PRIDEQ96B86.

Protocols and materials databases

DNASU56963.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000329082; ENSP00000330005; ENSG00000182175. [Q96B86-1]
ENST00000425933; ENSP00000404442; ENSG00000182175.
ENST00000542321; ENSP00000440025; ENSG00000182175.
ENST00000543599; ENSP00000442498; ENSG00000182175.
ENST00000557301; ENSP00000452126; ENSG00000182175.
GeneID56963.
KEGGhsa:56963.
UCSCuc002bsq.2. human. [Q96B86-1]

Organism-specific databases

CTD56963.
GeneCardsGC15M093586.
HGNCHGNC:30308. RGMA.
HPACAB012343.
MIM607362. gene.
neXtProtNX_Q96B86.
PharmGKBPA128394693.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG87951.
HOGENOMHOG000013072.
HOVERGENHBG057627.
InParanoidQ96B86.
KOK06847.
OMAPLNQQID.
OrthoDBEOG7VQJDC.
PhylomeDBQ96B86.
TreeFamTF329836.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ96B86.
BgeeQ96B86.
CleanExHS_RGMA.
GenevestigatorQ96B86.

Family and domain databases

InterProIPR009496. RGM_C.
IPR010536. RGM_N.
[Graphical view]
PfamPF06534. RGM_C. 1 hit.
PF06535. RGM_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRGMA. human.
GeneWikiRGMA.
GenomeRNAi56963.
NextBio35507888.
PROQ96B86.
SOURCESearch...

Entry information

Entry nameRGMA_HUMAN
AccessionPrimary (citable) accession number: Q96B86
Secondary accession number(s): B2RTW1 expand/collapse secondary AC list , B7Z5S8, F5GXQ7, F5GZU6, G3V518, Q0JV97, Q8NC80, Q9H0E6, Q9NPM3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM