Reviewed, UniProtKB/Swiss-Prot Q96B86 (RGMA_HUMAN)

Names and origin

Protein names

Recommended name:
    Repulsive guidance molecule A
Alternative name(s):
    RGM domain family member A

Gene names

Name:	RGMA
Synonyms:	RGM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Repulsive guidance molecules (Rgm) are involved in the guidance of growth cones of the developing neurons.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchorBy similarity.
Sequence similarities	Belongs to the repulsive guidance molecule (RGM) family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96B86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96B86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MQPP → ARGRGGRSLPARCSRRRSEAALSSQRDLFSSPFFLNSSS

Notes: Incomplete sequence. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 47	47	Potential
	Propeptide	48 – 168	121	Removed in mature form By similarity		PRO_0000030385
	Chain	169 – 424	256	Repulsive guidance molecule A		PRO_0000030386
	Propeptide	425 – 450	26	Removed in mature form Potential		PRO_0000030387
Amino acid modifications
	Lipidation	424	1	GPI-anchor amidated alanine Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Glycosylation	389	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 4	4	MQPP → ARGRGGRSLPARCSRRRSEA ALSSQRDLFSSPFFLNSSS in isoform 2.		VSP_022294
Experimental info
	Sequence conflict	199	1	V → A in BAC11285. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: E3244FA591182DCF	FASTA	450	49,361
	10 20 30 40 50 60 MQPPRERLVV TGRAGWMGMG RGAGRSALGF WPTLAFLLCS FPAATSPCKI LKCNSEFWSA 70 80 90 100 110 120 TSGSHAPASD DTPEFCAALR SYALCTRRTA RTCRGDLAYH SAVHGIEDLM SQHNCSKDGP 130 140 150 160 170 180 TSQPRLRTLP PAGDSQERSD SPEICHYEKS FHKHSATPNY THCGLFGDPH LRTFTDRFQT 190 200 210 220 230 240 CKVQGAWPLI DNNYLNVQVT NTPVLPGSAA TATSKLTIIF KNFQECVDQK VYQAEMDELP 250 260 270 280 290 300 AAFVDGSKNG GDKHGANSLK ITEKVSGQHV EIQAKYIGTT IVVRQVGRYL TFAVRMPEEV 310 320 330 340 350 360 VNAVEDWDSQ GLYLCLRGCP LNQQIDFQAF HTNAEGTGAR RLAAASPAPT APETFPYETA 370 380 390 400 410 420 VAKCKEKLPV EDLYYQACVF DLLTTGDVNF TLAAYYALED VKMLHSNKDK LHLYERTRDL 430 440 450 PGRAAAGLPL APRPLLGALV PLLALLPVFC
	Isoform 2 [UniParc]. Checksum: F0B61FB93BF262BE		485	53,161
	10 20 30 40 50 60 ARGRGGRSLP ARCSRRRSEA ALSSQRDLFS SPFFLNSSSR ERLVVTGRAG WMGMGRGAGR 70 80 90 100 110 120 SALGFWPTLA FLLCSFPAAT SPCKILKCNS EFWSATSGSH APASDDTPEF CAALRSYALC 130 140 150 160 170 180 TRRTARTCRG DLAYHSAVHG IEDLMSQHNC SKDGPTSQPR LRTLPPAGDS QERSDSPEIC 190 200 210 220 230 240 HYEKSFHKHS ATPNYTHCGL FGDPHLRTFT DRFQTCKVQG AWPLIDNNYL NVQVTNTPVL 250 260 270 280 290 300 PGSAATATSK LTIIFKNFQE CVDQKVYQAE MDELPAAFVD GSKNGGDKHG ANSLKITEKV 310 320 330 340 350 360 SGQHVEIQAK YIGTTIVVRQ VGRYLTFAVR MPEEVVNAVE DWDSQGLYLC LRGCPLNQQI 370 380 390 400 410 420 DFQAFHTNAE GTGARRLAAA SPAPTAPETF PYETAVAKCK EKLPVEDLYY QACVFDLLTT 430 440 450 460 470 480 GDVNFTLAAY YALEDVKMLH SNKDKLHLYE RTRDLPGRAA AGLPLAPRPL LGALVPLLAL LPVFC

References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[2]	The German cDNA consortium Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1) AND PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[4]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Retina.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL136826 mRNA. Translation: CAB66760.1. AM392685 mRNA. Translation: CAL37563.1. AK074910 mRNA. Translation: BAC11285.1. AK074966 mRNA. Translation: BAC11321.1. AK074980 mRNA. Translation: BAC11330.1. AL390083 mRNA. Translation: CAB98207.1. BC015886 mRNA. Translation: AAH15886.2.
RefSeq	NP_064596.1.
UniGene	Hs.271277
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96B86.
Genome annotation databases
Ensembl	ENSG00000182175. Homo sapiens. [Contig view]
GeneID	56963.
KEGG	hsa:56963.
Organism-specific databases
HGNC	HGNC:30308. RGMA.
HPA	CAB012343.
MIM	607362. gene.
PharmGKB	PA128394693.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q96B86.
HOVERGEN	Q96B86.
Gene expression databases
ArrayExpress	Q96B86.
CleanEx	HS_RGMA.
GermOnline	ENSG00000182175. Homo sapiens.
Family and domain databases
InterPro	IPR009496. RGM_C. IPR010536. RGM_N. [Graphical view]
Pfam	PF06534. RGM_C. 1 hit. PF06535. RGM_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	62595.
SOURCE	Search...

Entry information

Entry name

RGMA_HUMAN

Accession

Primary (citable) accession number: Q96B86
Secondary accession number(s): Q0JV97 , Q8NC80, Q9H0E6, Q9NPM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	November 4, 2008
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)