Reviewed, UniProtKB/Swiss-Prot Q96B23 (CR025_HUMAN)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein C18orf25

Gene names

Name:

C18orf25

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96B23-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96B23-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-299: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Uncharacterized protein C18orf25		PRO_0000079312
Regions
	Coiled coil	175 – 200	26	Potential
Amino acid modifications
	Modified residue	39	1	Phosphoserine Ref.7
	Modified residue	66	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9
	Modified residue	69	1	Phosphoserine Ref.7
	Modified residue	326	1	Phosphoserine Ref.6 Ref.9 Ref.10
	Modified residue	327	1	Phosphoserine Ref.6 Ref.9 Ref.10
	Modified residue	329	1	Phosphoserine Ref.6 Ref.9 Ref.10
Natural variations
	Alternative sequence	239 – 299	61	Missing in isoform 2.		VSP_014753
	Natural variant	313	1	S → SA: dbSNP rs34068795 and dbSNP rs59370768. Ref.1 Ref.2 Ref.4 Ref.5		VAR_046670
Experimental info
	Sequence conflict	233	1	I → V in CAD89920. Ref.2
	Sequence conflict	315	1	N → S in CAE45812. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 1843683941D4DD01	FASTA	403	43,324
	10 20 30 40 50 60 MKMEEAVGKV EELIESEAPP KASEQETAKE EDGSVELESQ VQKDGVADST VISSMPCLLM 70 80 90 100 110 120 ELRRDSSESQ LASTESDKPT TGRVYESDSS NHCMLSPSSS GHLADSDTLS SAEENEPSQA 130 140 150 160 170 180 ETAVEGDPSG VSGATVGRKS RRSRSESETS TMAAKKNRQS SDKQNGRVAK VKGHRSQKHK 190 200 210 220 230 240 ERIRLLRQKR EAAARKKYNL LQDSSTSDSD LTCDSSTSSS DDDEEVSGSS KTITAEIPDG 250 260 270 280 290 300 PPVVAHYDMS DTNSDPEVVN VDNLLAAAVV QEHSNSVGGQ DTGATWRTSG LLEELNAEAG 310 320 330 340 350 360 HLDPGFLASD KTSGNAPLNE EINIASSDSE VEIVGVQEHA RCVHPRGGVI QSVSSWKHGS 370 380 390 400 GTQYVSTRQT QSWTAVTPQQ TWASPAEVVD LTLDEDSRRK YLL
	Isoform 2. Checksum: CE45696435E5ED16	FASTA	342	36,977
	10 20 30 40 50 60 MKMEEAVGKV EELIESEAPP KASEQETAKE EDGSVELESQ VQKDGVADST VISSMPCLLM 70 80 90 100 110 120 ELRRDSSESQ LASTESDKPT TGRVYESDSS NHCMLSPSSS GHLADSDTLS SAEENEPSQA 130 140 150 160 170 180 ETAVEGDPSG VSGATVGRKS RRSRSESETS TMAAKKNRQS SDKQNGRVAK VKGHRSQKHK 190 200 210 220 230 240 ERIRLLRQKR EAAARKKYNL LQDSSTSDSD LTCDSSTSSS DDDEEVSGSS KTITAEIPGH 250 260 270 280 290 300 LDPGFLASDK TSGNAPLNEE INIASSDSEV EIVGVQEHAR CVHPRGGVIQ SVSSWKHGSG 310 320 330 340 TQYVSTRQTQ SWTAVTPQQT WASPAEVVDL TLDEDSRRKY LL

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-313 INS. Tissue: Brain and Trachea.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-313 INS. Tissue: Lymph node and Skeletal muscle.
[3]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-313 INS.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-313 INS. Tissue: Eye and Lung.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-326; SER-327 AND SER-329, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-66 AND SER-69, MASS SPECTROMETRY.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, MASS SPECTROMETRY.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-326; SER-327 AND SER-329, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; SER-327 AND SER-329, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK289738 mRNA. Translation: BAF82427.1. AK292981 mRNA. Translation: BAF85670.1. AL713661 mRNA. Translation: CAD28470.1. AL832027 mRNA. Translation: CAD89920.1. BX640683 mRNA. Translation: CAE45812.1. AC021763 Genomic DNA. No translation available. CH471088 Genomic DNA. Translation: EAX01473.1. BC016149 mRNA. Translation: AAH16149.1. BC084579 mRNA. Translation: AAH84579.1.
IPI	IPI00059687. IPI00152407.
RefSeq	NP_001008240.1. NP_659492.1.
UniGene	Hs.116486
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96B23. 1 interaction.
STRING	Q96B23.
PTM databases
PhosphoSite	Q96B23.
Proteomic databases
PRIDE	Q96B23.
Genome annotation databases
Ensembl	ENST00000282059; ENSP00000282059; ENSG00000152242; Homo sapiens. [Genome view]
GeneID	147339.
KEGG	hsa:147339.
UCSC	uc002lbw.1. human.
Organism-specific databases
CTD	147339.
GeneCards	GC18P042049.
HGNC	HGNC:28172. C18orf25.
PharmGKB	PA134919240.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q96B23.
InParanoid	Q96B23.
OMA	KQNGRVT.
OrthoDB	EOG9D81R8.
Gene expression databases
ArrayExpress	Q96B23.
Bgee	Q96B23.
CleanEx	HS_C18orf25.
Genevestigator	Q96B23.
GermOnline	ENSG00000152242. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	85587.

Entry information

Entry name

CR025_HUMAN

Accession

Primary (citable) accession number: Q96B23
Secondary accession number(s): A8K123 , A8KAB6, Q5XG78, Q6N058, Q86TB5, Q8TCQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 14, 2008
Last modified:	December 15, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.