Reviewed,
UniProtKB/Swiss-Prot Q96B01 (R51A1_HUMAN)
Last modified
May 26, 2009.
Version 58.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: RAD51-associated protein 1 Alternative name(s): RAD51-interacting protein | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 352 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA. Ref.1 |
| Subunit structure | |
| Subcellular location | Nucleus By similarity. Note: Colocalizes with RAD51 to multiple nuclear foci By similarity. |
| Tissue specificity | Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes. Ref.1 |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5 Ref.6 |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Ligand | DNA-binding RNA-binding |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | double-strand break repair via homologous recombination Ref.1 Non-traceable author statement. Source: UniProtKB |
| Cellular component | nucleus Ref.1 Inferred by curator. Source: UniProtKB |
| Molecular function | RNA binding Ref.1 Inferred from direct assay. Source: UniProtKB double-stranded DNA binding Ref.1Inferred from direct assay. Source: UniProtKB protein binding Ref.1Inferred from physical interaction. Source: UniProtKB single-stranded DNA binding Ref.1Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| RAD51 | Q06609 | 2 | EBI-1178743,EBI-297202 | |
| RAD51 | Q06609 | 3 | EBI-1178748,EBI-297202 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: Q96B01-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 2 Ref.1 (identifier: Q96B01-2) The sequence of this isoform differs from the canonical sequence as follows: 71-87: Missing. | ||||||
| Isoform 3 Ref.1 (identifier: Q96B01-3) The sequence of this isoform differs from the canonical sequence as follows: 258-307: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 352 | 352 | RAD51-associated protein 1 | PRO_0000097140 | |||||
Regions | |||||||||
| Region | 313 – 352 | 40 | Interaction with RAD51 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 19 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 21 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 66 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 120 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 280 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 327 | 1 | Phosphoserine Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 71 – 87 | 17 | Missing in isoform 2. Ref.1 | VSP_051739 | |||||
| Alternative sequence | 258 – 307 | 50 | Missing in isoform 3. Ref.1 | VSP_051740 | |||||
| Natural variant | 68 | 1 | K → Q: dbSNP rs34810644. | VAR_056976 | |||||
Experimental info | |||||||||
| Mutagenesis | 333 | 1 | R → A: Strongly descreases interaction with RAD51; when associated with Q-336; A-345 and A-346. Ref.4 | ||||||
| Mutagenesis | 336 | 1 | L → Q: Strongly descreases interaction with RAD51; when associated with A-333; A-345 and A-346. Ref.4 | ||||||
| Mutagenesis | 345 – 346 | 2 | LH → AA: Strongly descreases interaction with RAD51; when associated with A-333; and Q-336. | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A novel nucleic acid-binding protein that interacts with human rad51 recombinase." Kovalenko O.V., Golub E.I., Bray-Ward P., Ward D.C., Radding C.M. Nucleic Acids Res. 25:4946-4953(1997) [PubMed: 9396801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), FUNCTION, INTERACTION WITH RAD51, TISSUE SPECIFICITY. Tissue: Cervix carcinoma. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Bone marrow and Urinary bladder. |
| [4] | "RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51." Kovalenko O.V., Wiese C., Schild D. Nucleic Acids Res. 34:5081-5092(2006) [PubMed: 16990250] [Abstract] Cited for: INTERACTION WITH RAD51, MUTAGENESIS OF ARG-333; LEU-336 AND 345-LEU-HIS-346. |
| [5] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, MASS SPECTROMETRY. |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-21; THR-66; SER-280 AND SER-327, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF006259 mRNA. Translation: AAC39554.1. AK096930 mRNA. Translation: BAC04902.1. BC006992 mRNA. Translation: AAH06992.1. BC016330 mRNA. Translation: AAH16330.1. | |
| IPI | IPI00093253. IPI00396551. IPI00607574. |
| RefSeq | NP_001124334.1. |
| UniGene | Hs.709525 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q96B01. 4 interactions. |
PTM databases | |
| PhosphoSite | Q96B01. |
Proteomic databases | |
| PRIDE | Q96B01. |
Genome annotation databases | |
| Ensembl | ENSG00000111247. Homo sapiens. [Contig view] |
| GeneID | 10635. |
| KEGG | hsa:10635. |
Organism-specific databases | |
| GeneCards | GC12P004519. |
| H-InvDB | HIX0010349. |
| HGNC | HGNC:16956. RAD51AP1. |
| MIM | 603070. gene. |
| PharmGKB | PA134871784. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q96B01. |
| HOVERGEN | Q96B01. |
| OMA | Q96B01. KWVPPAA. |
Gene expression databases | |
| ArrayExpress | Q96B01. |
| Bgee | Q96B01. |
| CleanEx | HS_RAD51AP1. |
| GermOnline | ENSG00000111247. Homo sapiens. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 40421. |
| SOURCE | Search... |
Entry information
| Entry name | R51A1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q96B01 Secondary accession number(s): O43403, Q7Z779 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |

Clusters with


