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Q96B01

- R51A1_HUMAN

UniProt

Q96B01 - R51A1_HUMAN

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Protein
RAD51-associated protein 1
Gene
RAD51AP1, PIR51
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.2 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB
  2. double-stranded DNA binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. single-stranded DNA binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: UniProtKB
  2. cellular response to ionizing radiation Source: UniProtKB
  3. double-strand break repair via homologous recombination Source: UniProtKB
  4. regulation of double-strand break repair via homologous recombination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RAD51-associated protein 1
Alternative name(s):
RAD51-interacting protein
Gene namesi
Synonyms:PIR51
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:16956. RAD51AP1.

Subcellular locationi

Nucleus By similarity
Note: Colocalizes with RAD51 to multiple nuclear foci By similarity.

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi333 – 3331R → A: Strongly decreases interaction with RAD51; when associated with Q-336; A-345 and A-346. 1 Publication
Mutagenesisi336 – 3361L → Q: Strongly decreases interaction with RAD51; when associated with A-333; A-345 and A-346. 1 Publication
Mutagenesisi345 – 3462LH → AA: Strongly decreases interaction with RAD51; when associated with A-333; and Q-336.

Organism-specific databases

PharmGKBiPA134871784.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352RAD51-associated protein 1
PRO_0000097140Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine2 Publications
Modified residuei21 – 211Phosphoserine2 Publications
Modified residuei120 – 1201Phosphoserine2 Publications
Modified residuei280 – 2801Phosphoserine2 Publications
Modified residuei327 – 3271Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96B01.
PaxDbiQ96B01.
PRIDEiQ96B01.

PTM databases

PhosphoSiteiQ96B01.

Expressioni

Tissue specificityi

Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.1 Publication

Gene expression databases

ArrayExpressiQ96B01.
BgeeiQ96B01.
CleanExiHS_RAD51AP1.
GenevestigatoriQ96B01.

Organism-specific databases

HPAiHPA051499.

Interactioni

Subunit structurei

Isoform 2 interacts with RAD51. Interacts with PALB2. Interacts (via C-terminal region) with RAD51; the interaction is direct.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD51Q066095EBI-1178748,EBI-297202

Protein-protein interaction databases

BioGridi115879. 7 interactions.
DIPiDIP-35257N.
IntActiQ96B01. 6 interactions.
STRINGi9606.ENSP00000228843.

Structurei

3D structure databases

ProteinModelPortaliQ96B01.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 35240Interaction with RAD51
Add
BLAST

Phylogenomic databases

eggNOGiNOG28637.
HOGENOMiHOG000116153.
HOVERGENiHBG079699.
InParanoidiQ96B01.
OMAiDFCESED.
OrthoDBiEOG7GQXWK.
PhylomeDBiQ96B01.
TreeFamiTF335955.

Family and domain databases

InterProiIPR026632. RAD51-assoc_prot_1.
[Graphical view]
PANTHERiPTHR15361:SF4. PTHR15361:SF4. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q96B01-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVRPVRHKKP VNYSQFDHSD SDDDFVSATV PLNKKSRTAP KELKQDKPKP    50
NLNNLRKEEI PVQEKTPKKR LPEGTFSIPA SAVPCTKMAL DDKLYQRDLE 100
VALALSVKEL PTVTTNVQNS QDKSIEKHGS SKIETMNKSP HISNCSVASD 150
YLDLDKITVE DDVGGVQGKR KAASKAAAQQ RKILLEGSDG DSANDTEPDF 200
APGEDSEDDS DFCESEDNDE DFSMRKSKVK EIKKKEVKVK SPVEKKEKKS 250
KSKCNALVTS VDSAPAAVKS ESQSLPKKVS LSSDTTRKPL EIRSPSAESK 300
KPKWVPPAAS GGSRSSSSPL VVVSVKSPNQ SLRLGLSRLA RVKPLHPNAT 350
ST 352
Length:352
Mass (Da):38,457
Last modified:December 1, 2001 - v1
Checksum:iE582EE4BC459DD92
GO
Isoform 21 Publication (identifier: Q96B01-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-87: Missing.

Show »
Length:335
Mass (Da):36,757
Checksum:i71BBA478F55C5D39
GO
Isoform 31 Publication (identifier: Q96B01-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     258-307: Missing.

Show »
Length:302
Mass (Da):33,142
Checksum:iF667DBB6B8DF19E6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681K → Q.
Corresponds to variant rs34810644 [ dbSNP | Ensembl ].
VAR_056976

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei71 – 8717Missing in isoform 2. 1 Publication
VSP_051739Add
BLAST
Alternative sequencei258 – 30750Missing in isoform 3. 1 Publication
VSP_051740Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006259 mRNA. Translation: AAC39554.1.
AK096930 mRNA. Translation: BAC04902.1.
AK291948 mRNA. Translation: BAF84637.1.
CH471116 Genomic DNA. Translation: EAW88844.1.
BC006992 mRNA. Translation: AAH06992.1.
BC016330 mRNA. Translation: AAH16330.1.
CCDSiCCDS44805.1. [Q96B01-1]
CCDS8529.1. [Q96B01-2]
RefSeqiNP_001124334.1. NM_001130862.1. [Q96B01-1]
NP_006470.1. NM_006479.4. [Q96B01-2]
UniGeneiHs.730696.

Genome annotation databases

EnsembliENST00000228843; ENSP00000228843; ENSG00000111247. [Q96B01-1]
ENST00000321524; ENSP00000323750; ENSG00000111247. [Q96B01-3]
ENST00000352618; ENSP00000309479; ENSG00000111247. [Q96B01-2]
GeneIDi10635.
KEGGihsa:10635.
UCSCiuc001qmu.3. human. [Q96B01-2]
uc001qmw.3. human. [Q96B01-1]

Polymorphism databases

DMDMi68565925.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006259 mRNA. Translation: AAC39554.1 .
AK096930 mRNA. Translation: BAC04902.1 .
AK291948 mRNA. Translation: BAF84637.1 .
CH471116 Genomic DNA. Translation: EAW88844.1 .
BC006992 mRNA. Translation: AAH06992.1 .
BC016330 mRNA. Translation: AAH16330.1 .
CCDSi CCDS44805.1. [Q96B01-1 ]
CCDS8529.1. [Q96B01-2 ]
RefSeqi NP_001124334.1. NM_001130862.1. [Q96B01-1 ]
NP_006470.1. NM_006479.4. [Q96B01-2 ]
UniGenei Hs.730696.

3D structure databases

ProteinModelPortali Q96B01.
ModBasei Search...

Protein-protein interaction databases

BioGridi 115879. 7 interactions.
DIPi DIP-35257N.
IntActi Q96B01. 6 interactions.
STRINGi 9606.ENSP00000228843.

PTM databases

PhosphoSitei Q96B01.

Polymorphism databases

DMDMi 68565925.

Proteomic databases

MaxQBi Q96B01.
PaxDbi Q96B01.
PRIDEi Q96B01.

Protocols and materials databases

DNASUi 10635.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000228843 ; ENSP00000228843 ; ENSG00000111247 . [Q96B01-1 ]
ENST00000321524 ; ENSP00000323750 ; ENSG00000111247 . [Q96B01-3 ]
ENST00000352618 ; ENSP00000309479 ; ENSG00000111247 . [Q96B01-2 ]
GeneIDi 10635.
KEGGi hsa:10635.
UCSCi uc001qmu.3. human. [Q96B01-2 ]
uc001qmw.3. human. [Q96B01-1 ]

Organism-specific databases

CTDi 10635.
GeneCardsi GC12P004647.
HGNCi HGNC:16956. RAD51AP1.
HPAi HPA051499.
MIMi 603070. gene.
neXtProti NX_Q96B01.
PharmGKBi PA134871784.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG28637.
HOGENOMi HOG000116153.
HOVERGENi HBG079699.
InParanoidi Q96B01.
OMAi DFCESED.
OrthoDBi EOG7GQXWK.
PhylomeDBi Q96B01.
TreeFami TF335955.

Miscellaneous databases

ChiTaRSi RAD51AP1. human.
GenomeRNAii 10635.
NextBioi 40421.
PROi Q96B01.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q96B01.
Bgeei Q96B01.
CleanExi HS_RAD51AP1.
Genevestigatori Q96B01.

Family and domain databases

InterProi IPR026632. RAD51-assoc_prot_1.
[Graphical view ]
PANTHERi PTHR15361:SF4. PTHR15361:SF4. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel nucleic acid-binding protein that interacts with human rad51 recombinase."
    Kovalenko O.V., Golub E.I., Bray-Ward P., Ward D.C., Radding C.M.
    Nucleic Acids Res. 25:4946-4953(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), FUNCTION, INTERACTION WITH RAD51, TISSUE SPECIFICITY.
    Tissue: Cervix carcinoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Bone marrow and Urinary bladder.
  5. "RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51."
    Kovalenko O.V., Wiese C., Schild D.
    Nucleic Acids Res. 34:5081-5092(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51, MUTAGENESIS OF ARG-333; LEU-336 AND 345-LEU-HIS-346.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-21; SER-280 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION, INTERACTION WITH PALB2.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-21; SER-120 AND SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "FIGNL1-containing protein complex is required for efficient homologous recombination repair."
    Yuan J., Chen J.
    Proc. Natl. Acad. Sci. U.S.A. 110:10640-10645(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51.

Entry informationi

Entry nameiR51A1_HUMAN
AccessioniPrimary (citable) accession number: Q96B01
Secondary accession number(s): A8K7D3, O43403, Q7Z779
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

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