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Q96B01

- R51A1_HUMAN

UniProt

Q96B01 - R51A1_HUMAN

Protein

RAD51-associated protein 1

Gene

RAD51AP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.2 Publications

    GO - Molecular functioni

    1. double-stranded DNA binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB
    4. single-stranded DNA binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to ionizing radiation Source: UniProtKB
    2. DNA repair Source: UniProtKB
    3. double-strand break repair via homologous recombination Source: UniProtKB
    4. regulation of double-strand break repair via homologous recombination Source: UniProtKB

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RAD51-associated protein 1
    Alternative name(s):
    RAD51-interacting protein
    Gene namesi
    Name:RAD51AP1Imported
    Synonyms:PIR51
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:16956. RAD51AP1.

    Subcellular locationi

    Nucleus By similarity
    Note: Colocalizes with RAD51 to multiple nuclear foci.By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi333 – 3331R → A: Strongly decreases interaction with RAD51; when associated with Q-336; A-345 and A-346. 1 Publication
    Mutagenesisi336 – 3361L → Q: Strongly decreases interaction with RAD51; when associated with A-333; A-345 and A-346. 1 Publication
    Mutagenesisi345 – 3462LH → AA: Strongly decreases interaction with RAD51; when associated with A-333; and Q-336.

    Organism-specific databases

    PharmGKBiPA134871784.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352RAD51-associated protein 1PRO_0000097140Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphoserine2 Publications
    Modified residuei21 – 211Phosphoserine2 Publications
    Modified residuei120 – 1201Phosphoserine2 Publications
    Modified residuei280 – 2801Phosphoserine2 Publications
    Modified residuei327 – 3271Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ96B01.
    PaxDbiQ96B01.
    PRIDEiQ96B01.

    PTM databases

    PhosphoSiteiQ96B01.

    Expressioni

    Tissue specificityi

    Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.1 Publication

    Gene expression databases

    ArrayExpressiQ96B01.
    BgeeiQ96B01.
    CleanExiHS_RAD51AP1.
    GenevestigatoriQ96B01.

    Organism-specific databases

    HPAiHPA051499.

    Interactioni

    Subunit structurei

    Isoform 2 interacts with RAD51. Interacts with PALB2. Interacts (via C-terminal region) with RAD51; the interaction is direct.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAD51Q066095EBI-1178748,EBI-297202

    Protein-protein interaction databases

    BioGridi115879. 8 interactions.
    DIPiDIP-35257N.
    IntActiQ96B01. 6 interactions.
    STRINGi9606.ENSP00000228843.

    Structurei

    3D structure databases

    ProteinModelPortaliQ96B01.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni313 – 35240Interaction with RAD51Add
    BLAST

    Phylogenomic databases

    eggNOGiNOG28637.
    HOGENOMiHOG000116153.
    HOVERGENiHBG079699.
    InParanoidiQ96B01.
    OMAiDFCESED.
    OrthoDBiEOG7GQXWK.
    PhylomeDBiQ96B01.
    TreeFamiTF335955.

    Family and domain databases

    InterProiIPR026632. RAD51-assoc_prot_1.
    [Graphical view]
    PANTHERiPTHR15361:SF4. PTHR15361:SF4. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.1 Publication

    Isoform 1Curated (identifier: Q96B01-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVRPVRHKKP VNYSQFDHSD SDDDFVSATV PLNKKSRTAP KELKQDKPKP    50
    NLNNLRKEEI PVQEKTPKKR LPEGTFSIPA SAVPCTKMAL DDKLYQRDLE 100
    VALALSVKEL PTVTTNVQNS QDKSIEKHGS SKIETMNKSP HISNCSVASD 150
    YLDLDKITVE DDVGGVQGKR KAASKAAAQQ RKILLEGSDG DSANDTEPDF 200
    APGEDSEDDS DFCESEDNDE DFSMRKSKVK EIKKKEVKVK SPVEKKEKKS 250
    KSKCNALVTS VDSAPAAVKS ESQSLPKKVS LSSDTTRKPL EIRSPSAESK 300
    KPKWVPPAAS GGSRSSSSPL VVVSVKSPNQ SLRLGLSRLA RVKPLHPNAT 350
    ST 352
    Length:352
    Mass (Da):38,457
    Last modified:December 1, 2001 - v1
    Checksum:iE582EE4BC459DD92
    GO
    Isoform 21 Publication (identifier: Q96B01-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         71-87: Missing.

    Show »
    Length:335
    Mass (Da):36,757
    Checksum:i71BBA478F55C5D39
    GO
    Isoform 31 Publication (identifier: Q96B01-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         258-307: Missing.

    Show »
    Length:302
    Mass (Da):33,142
    Checksum:iF667DBB6B8DF19E6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti68 – 681K → Q.
    Corresponds to variant rs34810644 [ dbSNP | Ensembl ].
    VAR_056976

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei71 – 8717Missing in isoform 2. 3 PublicationsVSP_051739Add
    BLAST
    Alternative sequencei258 – 30750Missing in isoform 3. 1 PublicationVSP_051740Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006259 mRNA. Translation: AAC39554.1.
    AK096930 mRNA. Translation: BAC04902.1.
    AK291948 mRNA. Translation: BAF84637.1.
    CH471116 Genomic DNA. Translation: EAW88844.1.
    BC006992 mRNA. Translation: AAH06992.1.
    BC016330 mRNA. Translation: AAH16330.1.
    CCDSiCCDS44805.1. [Q96B01-1]
    CCDS8529.1. [Q96B01-2]
    RefSeqiNP_001124334.1. NM_001130862.1. [Q96B01-1]
    NP_006470.1. NM_006479.4. [Q96B01-2]
    UniGeneiHs.730696.

    Genome annotation databases

    EnsembliENST00000228843; ENSP00000228843; ENSG00000111247. [Q96B01-1]
    ENST00000352618; ENSP00000309479; ENSG00000111247. [Q96B01-2]
    GeneIDi10635.
    KEGGihsa:10635.
    UCSCiuc001qmu.3. human. [Q96B01-2]
    uc001qmw.3. human. [Q96B01-1]

    Polymorphism databases

    DMDMi68565925.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006259 mRNA. Translation: AAC39554.1 .
    AK096930 mRNA. Translation: BAC04902.1 .
    AK291948 mRNA. Translation: BAF84637.1 .
    CH471116 Genomic DNA. Translation: EAW88844.1 .
    BC006992 mRNA. Translation: AAH06992.1 .
    BC016330 mRNA. Translation: AAH16330.1 .
    CCDSi CCDS44805.1. [Q96B01-1 ]
    CCDS8529.1. [Q96B01-2 ]
    RefSeqi NP_001124334.1. NM_001130862.1. [Q96B01-1 ]
    NP_006470.1. NM_006479.4. [Q96B01-2 ]
    UniGenei Hs.730696.

    3D structure databases

    ProteinModelPortali Q96B01.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115879. 8 interactions.
    DIPi DIP-35257N.
    IntActi Q96B01. 6 interactions.
    STRINGi 9606.ENSP00000228843.

    PTM databases

    PhosphoSitei Q96B01.

    Polymorphism databases

    DMDMi 68565925.

    Proteomic databases

    MaxQBi Q96B01.
    PaxDbi Q96B01.
    PRIDEi Q96B01.

    Protocols and materials databases

    DNASUi 10635.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000228843 ; ENSP00000228843 ; ENSG00000111247 . [Q96B01-1 ]
    ENST00000352618 ; ENSP00000309479 ; ENSG00000111247 . [Q96B01-2 ]
    GeneIDi 10635.
    KEGGi hsa:10635.
    UCSCi uc001qmu.3. human. [Q96B01-2 ]
    uc001qmw.3. human. [Q96B01-1 ]

    Organism-specific databases

    CTDi 10635.
    GeneCardsi GC12P004647.
    HGNCi HGNC:16956. RAD51AP1.
    HPAi HPA051499.
    MIMi 603070. gene.
    neXtProti NX_Q96B01.
    PharmGKBi PA134871784.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG28637.
    HOGENOMi HOG000116153.
    HOVERGENi HBG079699.
    InParanoidi Q96B01.
    OMAi DFCESED.
    OrthoDBi EOG7GQXWK.
    PhylomeDBi Q96B01.
    TreeFami TF335955.

    Miscellaneous databases

    ChiTaRSi RAD51AP1. human.
    GenomeRNAii 10635.
    NextBioi 40421.
    PROi Q96B01.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96B01.
    Bgeei Q96B01.
    CleanExi HS_RAD51AP1.
    Genevestigatori Q96B01.

    Family and domain databases

    InterProi IPR026632. RAD51-assoc_prot_1.
    [Graphical view ]
    PANTHERi PTHR15361:SF4. PTHR15361:SF4. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel nucleic acid-binding protein that interacts with human rad51 recombinase."
      Kovalenko O.V., Golub E.I., Bray-Ward P., Ward D.C., Radding C.M.
      Nucleic Acids Res. 25:4946-4953(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), FUNCTION, INTERACTION WITH RAD51, TISSUE SPECIFICITY.
      Tissue: Cervix carcinoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Bone marrowImported and Urinary bladderImported.
    5. "RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51."
      Kovalenko O.V., Wiese C., Schild D.
      Nucleic Acids Res. 34:5081-5092(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAD51, MUTAGENESIS OF ARG-333; LEU-336 AND 345-LEU-HIS-346.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-21; SER-280 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: FUNCTION, INTERACTION WITH PALB2.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-21; SER-120 AND SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "FIGNL1-containing protein complex is required for efficient homologous recombination repair."
      Yuan J., Chen J.
      Proc. Natl. Acad. Sci. U.S.A. 110:10640-10645(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAD51.

    Entry informationi

    Entry nameiR51A1_HUMAN
    AccessioniPrimary (citable) accession number: Q96B01
    Secondary accession number(s): A8K7D3, O43403, Q7Z779
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3