Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-lysine methyltransferase METTL21B

Gene

METTL21B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protein-lysine methyltransferase.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei104 – 1041S-adenosyl-L-methionineBy similarity
Binding sitei133 – 1331S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei150 – 1501S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine methyltransferase METTL21B (EC:2.1.1.-)
Alternative name(s):
Hepatocellular carcinoma-associated antigen 557a
Methyltransferase-like protein 21B
Gene namesi
Name:METTL21B
Synonyms:FAM119B, HCA557A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:24936. METTL21B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145008445.

Polymorphism and mutation databases

BioMutaiMETTL21B.
DMDMi74731178.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Protein-lysine methyltransferase METTL21BPRO_0000291850Add
BLAST

Proteomic databases

MaxQBiQ96AZ1.
PaxDbiQ96AZ1.
PRIDEiQ96AZ1.

Expressioni

Gene expression databases

BgeeiQ96AZ1.
CleanExiHS_FAM119B.
ExpressionAtlasiQ96AZ1. baseline and differential.
GenevisibleiQ96AZ1. HS.

Organism-specific databases

HPAiHPA043020.

Interactioni

Subunit structurei

Interacts with members of the heat shock protein 70 and 90 families and of the TCP-1 chaperonin family, as well as with HSPD1, STIP1 and tubulin; at least some of these proteins may be methylation substrates.1 Publication

Protein-protein interaction databases

BioGridi117403. 59 interactions.
MINTiMINT-2813700.
STRINGi9606.ENSP00000300209.

Structurei

Secondary structure

1
226
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 359Combined sources
Beta strandi38 – 4912Combined sources
Helixi51 – 544Combined sources
Helixi58 – 6912Combined sources
Beta strandi78 – 836Combined sources
Helixi88 – 958Combined sources
Beta strandi99 – 1046Combined sources
Helixi106 – 1083Combined sources
Helixi109 – 11911Combined sources
Beta strandi125 – 1306Combined sources
Turni133 – 1353Combined sources
Helixi137 – 1393Combined sources
Beta strandi144 – 1518Combined sources
Helixi156 – 1583Combined sources
Helixi159 – 16911Combined sources
Beta strandi170 – 18112Combined sources
Helixi184 – 1863Combined sources
Helixi188 – 1936Combined sources
Helixi196 – 1983Combined sources
Beta strandi200 – 2089Combined sources
Turni209 – 2124Combined sources
Beta strandi213 – 2208Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QPNX-ray1.25A1-226[»]
ProteinModelPortaliQ96AZ1.
SMRiQ96AZ1. Positions 24-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 853S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410ISZA. Eukaryota.
ENOG4111N17. LUCA.
GeneTreeiENSGT00550000074572.
HOVERGENiHBG059353.
InParanoidiQ96AZ1.
OMAiRAQVCAL.
PhylomeDBiQ96AZ1.
TreeFamiTF313206.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96AZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADPGPDPES ESESVFPREV GLFADSYSEK SQFCFCGHVL TITQNFGSRL
60 70 80 90 100
GVAARVWDAA LSLCNYFESQ NVDFRGKKVI ELGAGTGIVG ILAALQGGDV
110 120 130 140 150
TITDLPLALE QIQGNVQANV PAGGQAQVRA LSWGIDHHVF PANYDLVLGA
160 170 180 190 200
DIVYLEPTFP LLLGTLQHLC RPHGTIYLAS KMRKEHGTES FFQHLLPQHF
210 220
QLELAQRDED ENVNIYRARH REPRPA
Length:226
Mass (Da):24,911
Last modified:December 1, 2001 - v1
Checksum:iA73C4B00AF18B10B
GO
Isoform 2 (identifier: Q96AZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-149: GDVTITDLPL...FPANYDLVLG → AYGLVRETED...SSVRGGCYHH
     150-226: Missing.

Show »
Length:149
Mass (Da):16,154
Checksum:i70254A97063C4A09
GO
Isoform 3 (identifier: Q96AZ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: Missing.
     72-97: VDFRGKKVIELGAGTGIVGILAALQG → MRGACGHALSMSTMTPWESIKGSSVR

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,317
Checksum:iB2551D7D107A06B5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281S → L.
Corresponds to variant rs34913183 [ dbSNP | Ensembl ].
VAR_032869

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7171Missing in isoform 3. 1 PublicationVSP_026268Add
BLAST
Alternative sequencei72 – 9726VDFRG…AALQG → MRGACGHALSMSTMTPWESI KGSSVR in isoform 3. 1 PublicationVSP_026269Add
BLAST
Alternative sequencei98 – 14952GDVTI…DLVLG → AYGLVRETEDDVIEQELWRG MRGACGHALSMSTMTPWESI KGSSVRGGCYHH in isoform 2. 1 PublicationVSP_026270Add
BLAST
Alternative sequencei150 – 22677Missing in isoform 2. 1 PublicationVSP_026271Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455816 mRNA. Translation: AAL66294.1.
AK024983 mRNA. Translation: BAB15049.1.
AL050100 mRNA. Translation: CAB43271.2.
BC016395 mRNA. Translation: AAH16395.1.
BC099841 mRNA. Translation: AAH99841.1.
BC103501 mRNA. Translation: AAI03502.1.
CCDSiCCDS31848.1. [Q96AZ1-2]
CCDS8957.1. [Q96AZ1-1]
PIRiT08749.
RefSeqiNP_056248.2. NM_015433.2. [Q96AZ1-1]
NP_996797.1. NM_206914.1. [Q96AZ1-2]
UniGeneiHs.632720.

Genome annotation databases

EnsembliENST00000300209; ENSP00000300209; ENSG00000123427. [Q96AZ1-1]
ENST00000333012; ENSP00000327425; ENSG00000123427. [Q96AZ1-2]
GeneIDi25895.
KEGGihsa:25895.
UCSCiuc001sqf.4. human. [Q96AZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455816 mRNA. Translation: AAL66294.1.
AK024983 mRNA. Translation: BAB15049.1.
AL050100 mRNA. Translation: CAB43271.2.
BC016395 mRNA. Translation: AAH16395.1.
BC099841 mRNA. Translation: AAH99841.1.
BC103501 mRNA. Translation: AAI03502.1.
CCDSiCCDS31848.1. [Q96AZ1-2]
CCDS8957.1. [Q96AZ1-1]
PIRiT08749.
RefSeqiNP_056248.2. NM_015433.2. [Q96AZ1-1]
NP_996797.1. NM_206914.1. [Q96AZ1-2]
UniGeneiHs.632720.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QPNX-ray1.25A1-226[»]
ProteinModelPortaliQ96AZ1.
SMRiQ96AZ1. Positions 24-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117403. 59 interactions.
MINTiMINT-2813700.
STRINGi9606.ENSP00000300209.

Polymorphism and mutation databases

BioMutaiMETTL21B.
DMDMi74731178.

Proteomic databases

MaxQBiQ96AZ1.
PaxDbiQ96AZ1.
PRIDEiQ96AZ1.

Protocols and materials databases

DNASUi25895.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300209; ENSP00000300209; ENSG00000123427. [Q96AZ1-1]
ENST00000333012; ENSP00000327425; ENSG00000123427. [Q96AZ1-2]
GeneIDi25895.
KEGGihsa:25895.
UCSCiuc001sqf.4. human. [Q96AZ1-1]

Organism-specific databases

CTDi25895.
GeneCardsiMETTL21B.
HGNCiHGNC:24936. METTL21B.
HPAiHPA043020.
MIMi615258. gene.
neXtProtiNX_Q96AZ1.
PharmGKBiPA145008445.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISZA. Eukaryota.
ENOG4111N17. LUCA.
GeneTreeiENSGT00550000074572.
HOVERGENiHBG059353.
InParanoidiQ96AZ1.
OMAiRAQVCAL.
PhylomeDBiQ96AZ1.
TreeFamiTF313206.

Miscellaneous databases

GenomeRNAii25895.
NextBioi47335.
PROiQ96AZ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96AZ1.
CleanExiHS_FAM119B.
ExpressionAtlasiQ96AZ1. baseline and differential.
GenevisibleiQ96AZ1. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HCA557a, a transcription factor associated with hepatocellular carcinoma."
    Dong X.-Y., Chen W.-F.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Colon.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity."
    Cloutier P., Lavallee-Adam M., Faubert D., Blanchette M., Coulombe B.
    PLoS Genet. 9:E1003210-E1003210(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSP70 FAMILY MEMBERS; TCP-1 CHAPERONIN FAMILY MEMBERS; HSPD1; STIP1 AND TUBULIN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMT21B_HUMAN
AccessioniPrimary (citable) accession number: Q96AZ1
Secondary accession number(s): Q9H749, Q9Y3W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: December 1, 2001
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.