Q96AX1 (VP33A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 33A
Short name=hVPS33A

Gene names

Name:

VPS33A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes By similarity.
Subunit structure	Large heterooligomeric complex together with VPS11, VPS16 and VPS18. Interacts with STX7 By similarity. Interacts with VIPAS39. Ref.5
Subcellular location	Late endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes By similarity.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.
Sequence caution	The sequence BAB15570.1 differs from that shown. Reason: Frameshift at position 140.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Lysosome Membrane
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysosome localization Inferred from direct assay PubMed 15790593. Source: UniProtKB melanosome localization Inferred from direct assay PubMed 15790593. Source: UniProtKB platelet formation Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of developmental pigmentation Inferred from sequence or structural similarity. Source: UniProtKB vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from direct assay PubMed 15790593. Source: MGI
Cellular_component	early endosome Inferred from direct assay Ref.5. Source: UniProtKB late endosome Inferred from direct assay Ref.5. Source: UniProtKB late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Vacuolar protein sorting-associated protein 33A		PRO_0000206302
Natural variations
	Natural variant	256	1	I → L. Corresponds to variant rs34996966 [ dbSNP | Ensembl ].		VAR_052471

Sequences

	Sequence		Length	Mass (Da)
	Q96AX1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 12442A1D9A223F56	FASTA	596	67,611
	10 20 30 40 50 60 MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA QYSLLKEHEV 70 80 90 100 110 120 EKMFTLKGNR LPAADVKNII FFVRPRLELM DIIAENVLSE DRRGPTRDFH ILFVPRRSLL 130 140 150 160 170 180 CEQRLKDLGV LGSFIHREEY SLDLIPFDGD LLSMESEGAF KECYLEGDQT SLYHAAKGLM 190 200 210 220 230 240 TLQALYGTIP QIFGKGECAR QVANMMIRMK REFTGSQNSI FPVFDNLLLL DRNVDLLTPL 250 260 270 280 290 300 ATQLTYEGLI DEIYGIQNSY VKLPPEKFAP KKQGDGGKDL PTEAKKLQLN SAEELYAEIR 310 320 330 340 350 360 DKNFNAVGSV LSKKAKIISA AFEERHNAKT VGEIKQFVSQ LPHMQAARGS LANHTSIAEL 370 380 390 400 410 420 IKDVTTSEDF FDKLTVEQEF MSGIDTDKVN NYIEDCIAQK HSLIKVLRLV CLQSVCNSGL 430 440 450 460 470 480 KQKVLDYYKR EILQTYGYEH ILTLHNLEKA GLLKPQTGGR NNYPTIRKTL RLWMDDVNEQ 490 500 510 520 530 540 NPTDISYVYS GYAPLSVRLA QLLSRPGWRS IEEVLRILPG PHFEERQPLP TGLQKKRQPG 550 560 570 580 590 ENRVTLIFFL GGVTFAEIAA LRFLSQLEDG GTEYVIATTK LMNGTSWIEA LMEKPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse organellar biogenesis mutant buff results from a mutation in Vps33a, a homologue of yeast vps33 and Drosophila carnation." Suzuki T., Oiso N., Gautam R., Novak E.K., Panthier J.J., Suprabha P.G., Vida T., Swank R.T., Spritz R.A. Proc. Natl. Acad. Sci. U.S.A. 100:1146-1150(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[5]	"SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery." Zhu G.D., Salazar G., Zlatic S.A., Fiza B., Doucette M.M., Heilman C.J., Levey A.I., Faundez V., L'hernault S.W. Mol. Biol. Cell 20:1223-1240(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VIPAS39.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF439857 mRNA. Translation: AAL33577.1. AK026840 mRNA. Translation: BAB15570.1. Frameshift. CH471054 Genomic DNA. Translation: EAW98319.1. BC016617 mRNA. Translation: AAH16617.1.
IPI	IPI00073179.
RefSeq	NP_075067.2. NM_022916.4.
UniGene	Hs.592009.
3D structure databases
ProteinModelPortal	Q96AX1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96AX1. 6 interactions.
STRING	9606.ENSP00000267199.
PTM databases
PhosphoSite	Q96AX1.
Polymorphism databases
DMDM	23396917.
Proteomic databases
PaxDb	Q96AX1.
PRIDE	Q96AX1.
Protocols and materials databases
DNASU	65082.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267199; ENSP00000267199; ENSG00000139719.
GeneID	65082.
KEGG	hsa:65082.
UCSC	uc001ucd.3. human.
Organism-specific databases
CTD	65082.
GeneCards	GC12M122716.
H-InvDB	HIX0079486.
HGNC	HGNC:18179. VPS33A.
HPA	HPA038701.
MIM	610034. gene.
neXtProt	NX_Q96AX1.
PharmGKB	PA38306.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5158.
HOGENOM	HOG000166771.
HOVERGEN	HBG054244.
InParanoid	Q96AX1.
OMA	ECYLEND.
OrthoDB	EOG42FSHB.
PhylomeDB	Q96AX1.
Gene expression databases
ArrayExpress	Q96AX1.
Bgee	Q96AX1.
CleanEx	HS_VPS33A.
Genevestigator	Q96AX1.
GermOnline	ENSG00000139719. Homo sapiens.
Family and domain databases
InterPro	IPR001619. Sec1-like. IPR027121. VPS33. [Graphical view]
PANTHER	PTHR11679. PTHR11679. 1 hit. PTHR11679:SF1. PTHR11679:SF1. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	VPS33A. human.
GenomeRNAi	65082.
NextBio	67276.
SOURCE	Search...

Entry information

Entry name

VP33A_HUMAN

Accession

Primary (citable) accession number: Q96AX1
Secondary accession number(s): Q547V4, Q9H5Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.