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Protein

Vesicular, overexpressed in cancer, prosurvival protein 1

Gene

VOPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Increases the transcriptional activity of NFKB1 by facilitating its nuclear translocation, DNA-binding and associated apoptotic response, when overexpressed.1 Publication

GO - Molecular functioni

  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • signal transduction Source: GOC
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular, overexpressed in cancer, prosurvival protein 1
Alternative name(s):
EGFR-coamplified and overexpressed protein
Short name:
ECop
Glioblastoma-amplified secreted protein
Putative NF-kappa-B-activating protein 055N
Gene namesi
Name:VOPP1
Synonyms:ECOP, GASP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:34518. VOPP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 6038ExtracellularSequence analysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Topological domaini82 – 17291CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB
  • endosome Source: LIFEdb
  • integral component of organelle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165618503.

Polymorphism and mutation databases

BioMutaiVOPP1.
DMDMi74760714.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 172150Vesicular, overexpressed in cancer, prosurvival protein 1PRO_0000325917Add
BLAST

Proteomic databases

PaxDbiQ96AW1.
PRIDEiQ96AW1.

PTM databases

iPTMnetiQ96AW1.
PhosphoSiteiQ96AW1.

Expressioni

Tissue specificityi

Widely expressed with highest levels in thymus and ovary.1 Publication

Gene expression databases

BgeeiQ96AW1.
ExpressionAtlasiQ96AW1. baseline and differential.
GenevisibleiQ96AW1. HS.

Organism-specific databases

HPAiHPA016826.

Interactioni

Protein-protein interaction databases

IntActiQ96AW1. 16 interactions.
MINTiMINT-4723663.
STRINGi9606.ENSP00000285279.

Structurei

3D structure databases

ProteinModelPortaliQ96AW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi87 – 16478Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the VOPP1/ECOP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVPU. Eukaryota.
ENOG4111SC5. LUCA.
GeneTreeiENSGT00390000015821.
HOGENOMiHOG000065719.
InParanoidiQ96AW1.
OMAiPYEDCCG.
OrthoDBiEOG7Z3F6B.
PhylomeDBiQ96AW1.
TreeFamiTF332098.

Family and domain databases

InterProiIPR026229. VOPP1.
[Graphical view]
PRINTSiPR02068. VOPPROTEIN1.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96AW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRQPAKVAA LLLGLLLECT EAKKHCWYFE GLYPTYYICR SYEDCCGSRC
60 70 80 90 100
CVRALSIQRL WYFWFLLMMG VLFCCGAGFF IRRRMYPPPL IEEPAFNVSY
110 120 130 140 150
TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA
160 170
CPPPPAYCNT PPPPYEQVVK AK
Length:172
Mass (Da):19,224
Last modified:December 1, 2001 - v1
Checksum:i7F7F75231C2A4276
GO
Isoform 2 (identifier: Q96AW1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-18: QPAKVAALLLGLLLE → TGSHAQ

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,275
Checksum:i3D8E449D6A98A9C8
GO
Isoform 3 (identifier: Q96AW1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-18: RRQPAKVAALLLGLLLE → LSETLGYLSSVLLQ

Note: No experimental confirmation available.
Show »
Length:169
Mass (Da):18,886
Checksum:i472021D3ECE5C0E5
GO
Isoform 4 (identifier: Q96AW1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MRRQPAKVAALLLGLLLE → M

Note: Gene prediction based on EST data.
Show »
Length:155
Mass (Da):17,381
Checksum:iD522150B1C5E469B
GO

Sequence cautioni

The sequence AAK82888.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921E → K in CAG38514 (Ref. 4) Curated
Sequence conflicti111 – 1111A → T in BAC11590 (PubMed:14702039).Curated
Sequence conflicti134 – 1341M → T in CAB66545 (PubMed:12761501).Curated
Sequence conflicti134 – 1341M → T in CAG38514 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1818MRRQP…GLLLE → M in isoform 4. CuratedVSP_055723Add
BLAST
Alternative sequencei2 – 1817RRQPA…GLLLE → LSETLGYLSSVLLQ in isoform 3. 1 PublicationVSP_043867Add
BLAST
Alternative sequencei4 – 1815QPAKV…GLLLE → TGSHAQ in isoform 2. 1 PublicationVSP_043868Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136610 mRNA. Translation: CAB66545.1.
AB097004 mRNA. Translation: BAC77357.1.
AK075391 mRNA. Translation: BAC11590.1.
AK092992 mRNA. Translation: BAG52634.1.
AK314338 mRNA. Translation: BAG36981.1.
AK315882 mRNA. Translation: BAF98773.1.
CR533483 mRNA. Translation: CAG38514.1.
AC073237 Genomic DNA. No translation available.
AC073347 Genomic DNA. No translation available.
AC099681 Genomic DNA. No translation available.
CH236957 Genomic DNA. Translation: EAL23811.1.
CH471201 Genomic DNA. Translation: EAW50969.1.
BC016650 mRNA. Translation: AAH16650.1.
AF395824 mRNA. Translation: AAK82888.1. Sequence problems.
CCDSiCCDS47588.1. [Q96AW1-1]
CCDS64654.1. [Q96AW1-2]
CCDS64655.1. [Q96AW1-3]
CCDS64656.1. [Q96AW1-4]
RefSeqiNP_001271211.1. NM_001284282.1. [Q96AW1-4]
NP_001271212.1. NM_001284283.1. [Q96AW1-3]
NP_001271213.1. NM_001284284.1. [Q96AW1-2]
NP_110423.3. NM_030796.4. [Q96AW1-1]
XP_011513844.1. XM_011515542.1.
XP_011513846.1. XM_011515544.1.
XP_011513847.1. XM_011515545.1.
UniGeneiHs.488307.
Hs.680358.

Genome annotation databases

EnsembliENST00000285279; ENSP00000285279; ENSG00000154978. [Q96AW1-1]
ENST00000418904; ENSP00000393210; ENSG00000154978. [Q96AW1-4]
ENST00000433959; ENSP00000415230; ENSG00000154978. [Q96AW1-2]
ENST00000625836; ENSP00000486983; ENSG00000154978. [Q96AW1-3]
GeneIDi81552.
KEGGihsa:81552.
UCSCiuc003tqq.5. human. [Q96AW1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136610 mRNA. Translation: CAB66545.1.
AB097004 mRNA. Translation: BAC77357.1.
AK075391 mRNA. Translation: BAC11590.1.
AK092992 mRNA. Translation: BAG52634.1.
AK314338 mRNA. Translation: BAG36981.1.
AK315882 mRNA. Translation: BAF98773.1.
CR533483 mRNA. Translation: CAG38514.1.
AC073237 Genomic DNA. No translation available.
AC073347 Genomic DNA. No translation available.
AC099681 Genomic DNA. No translation available.
CH236957 Genomic DNA. Translation: EAL23811.1.
CH471201 Genomic DNA. Translation: EAW50969.1.
BC016650 mRNA. Translation: AAH16650.1.
AF395824 mRNA. Translation: AAK82888.1. Sequence problems.
CCDSiCCDS47588.1. [Q96AW1-1]
CCDS64654.1. [Q96AW1-2]
CCDS64655.1. [Q96AW1-3]
CCDS64656.1. [Q96AW1-4]
RefSeqiNP_001271211.1. NM_001284282.1. [Q96AW1-4]
NP_001271212.1. NM_001284283.1. [Q96AW1-3]
NP_001271213.1. NM_001284284.1. [Q96AW1-2]
NP_110423.3. NM_030796.4. [Q96AW1-1]
XP_011513844.1. XM_011515542.1.
XP_011513846.1. XM_011515544.1.
XP_011513847.1. XM_011515545.1.
UniGeneiHs.488307.
Hs.680358.

3D structure databases

ProteinModelPortaliQ96AW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ96AW1. 16 interactions.
MINTiMINT-4723663.
STRINGi9606.ENSP00000285279.

PTM databases

iPTMnetiQ96AW1.
PhosphoSiteiQ96AW1.

Polymorphism and mutation databases

BioMutaiVOPP1.
DMDMi74760714.

Proteomic databases

PaxDbiQ96AW1.
PRIDEiQ96AW1.

Protocols and materials databases

DNASUi81552.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000285279; ENSP00000285279; ENSG00000154978. [Q96AW1-1]
ENST00000418904; ENSP00000393210; ENSG00000154978. [Q96AW1-4]
ENST00000433959; ENSP00000415230; ENSG00000154978. [Q96AW1-2]
ENST00000625836; ENSP00000486983; ENSG00000154978. [Q96AW1-3]
GeneIDi81552.
KEGGihsa:81552.
UCSCiuc003tqq.5. human. [Q96AW1-1]

Organism-specific databases

CTDi81552.
GeneCardsiVOPP1.
HGNCiHGNC:34518. VOPP1.
HPAiHPA016826.
MIMi611915. gene.
neXtProtiNX_Q96AW1.
PharmGKBiPA165618503.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVPU. Eukaryota.
ENOG4111SC5. LUCA.
GeneTreeiENSGT00390000015821.
HOGENOMiHOG000065719.
InParanoidiQ96AW1.
OMAiPYEDCCG.
OrthoDBiEOG7Z3F6B.
PhylomeDBiQ96AW1.
TreeFamiTF332098.

Miscellaneous databases

ChiTaRSiVOPP1. human.
GeneWikiiECOP.
GenomeRNAii81552.
PROiQ96AW1.
SOURCEiSearch...

Gene expression databases

BgeeiQ96AW1.
ExpressionAtlasiQ96AW1. baseline and differential.
GenevisibleiQ96AW1. HS.

Family and domain databases

InterProiIPR026229. VOPP1.
[Graphical view]
PRINTSiPR02068. VOPPROTEIN1.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
    Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
    Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung fibroblast.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Spleen, Synovial cell and Umbilical cord blood.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  9. "A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma."
    Eley G.D., Reiter J.L., Pandita A., Park S., Jenkins R.B., Maihle N.J., James C.D.
    Neuro-oncol. 4:86-94(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-172 (ISOFORM 1), TISSUE SPECIFICITY.
  10. "ECop (EGFR-coamplified and overexpressed protein), a novel protein, regulates NF-kappaB transcriptional activity and associated apoptotic response in an IkappaBalpha-dependent manner."
    Park S., James C.D.
    Oncogene 24:2495-2502(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Intracellular localization of GASP/ECOP/VOPP1."
    Baras A., Moskaluk C.A.
    J. Mol. Histol. 41:153-164(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiVOPP1_HUMAN
AccessioniPrimary (citable) accession number: Q96AW1
Secondary accession number(s): B0AZU1
, B2RAT4, B3KS72, C9JWR3, Q6FIE3, Q8NBN8, Q96RE5, Q9H0W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.