Q96AW1 (VOPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicular, overexpressed in cancer, prosurvival protein 1

Alternative name(s):
EGFR-coamplified and overexpressed protein
Short name=ECop
Glioblastoma-amplified secreted protein
Putative NF-kappa-B-activating protein 055N

Gene names

Name:	VOPP1
Synonyms:	ECOP, GASP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Increases the transcriptional activity of NFKB1 by facilitating its nuclear translocation, DNA-binding and associated apoptotic response, when overexpressed. Ref.9
Subcellular location	Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Note: When overexpressed, localizes in the nucleus and perinuclear regions. Ref.10
Sequence similarities	Belongs to the VOPP1/ECOP family.
Sequence caution	The sequence AAK82888.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasmic vesicle Membrane
Domain	Signal Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle membrane Inferred from direct assay Ref.10. Source: UniProtKB endosome Inferred from direct assay. Source: LIFEdb integral to organelle membrane Inferred from direct assay Ref.10. Source: UniProtKB
Molecular function	signal transducer activity Inferred from mutant phenotype Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 172	150	Vesicular, overexpressed in cancer, prosurvival protein 1		PRO_0000325917
Regions
	Topological domain	23 – 60	38	Extracellular Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Topological domain	82 – 172	91	Cytoplasmic Potential
	Compositional bias	87 – 164	78	Pro-rich
Experimental info
	Sequence conflict	92	1	E → K in CAG38514. Ref.4
	Sequence conflict	111	1	A → T in BAC11590. Ref.3
	Sequence conflict	134	1	M → T in CAB66545. Ref.2
	Sequence conflict	134	1	M → T in CAG38514. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q96AW1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7F7F75231C2A4276	FASTA	172	19,224
	10 20 30 40 50 60 MRRQPAKVAA LLLGLLLECT EAKKHCWYFE GLYPTYYICR SYEDCCGSRC CVRALSIQRL 70 80 90 100 110 120 WYFWFLLMMG VLFCCGAGFF IRRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT 130 140 150 160 170 DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed: 12761501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung fibroblast.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Synovial cell.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[8]	"A 2 Mb physical and transcript map of the 7p11.2 region surrounding the EGFR locus amplified in glioblastomas." Eley G.D., Wang X.Y., Frederick L., Hebrink D., Nash C., James C.D. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-172.
[9]	"ECop (EGFR-coamplified and overexpressed protein), a novel protein, regulates NF-kappaB transcriptional activity and associated apoptotic response in an IkappaBalpha-dependent manner." Park S., James C.D. Oncogene 24:2495-2502(2005) [PubMed: 15735698] [Abstract] Cited for: FUNCTION.
[10]	"Intracellular localization of GASP/ECOP/VOPP1." Baras A., Moskaluk C.A. J. Mol. Histol. 41:153-164(2010) [PubMed: 20571887] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136610 mRNA. Translation: CAB66545.1. AB097004 mRNA. Translation: BAC77357.1. AK075391 mRNA. Translation: BAC11590.1. AK314338 mRNA. Translation: BAG36981.1. CR533483 mRNA. Translation: CAG38514.1. CH236957 Genomic DNA. Translation: EAL23811.1. CH471201 Genomic DNA. Translation: EAW50969.1. BC016650 mRNA. Translation: AAH16650.1. AF395824 mRNA. Translation: AAK82888.1. Sequence problems.
IPI	IPI00414382.
RefSeq	NP_110423.3. NM_030796.3.
UniGene	Hs.488307.
3D structure databases
ProteinModelPortal	Q96AW1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96AW1. 16 interactions.
STRING	Q96AW1.
Proteomic databases
PRIDE	Q96AW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000285279; ENSP00000285279; ENSG00000154978.
GeneID	81552.
KEGG	hsa:81552.
UCSC	uc003tqs.1. human.
Organism-specific databases
CTD	81552.
GeneCards	GC07M055503.
H-InvDB	HIX0006698.
HGNC	HGNC:34518. VOPP1.
HPA	HPA016826.
neXtProt	NX_Q96AW1.
PharmGKB	PA165618503.
GenAtlas	Search...
Phylogenomic databases
eggNOG	maNOG22837.
GeneTree	ENSGT00390000015821.
HOGENOM	HBG445635.
OMA	YEQVVKS.
OrthoDB	EOG48GW4N.
PhylomeDB	Q96AW1.
Gene expression databases
ArrayExpress	Q96AW1.
Bgee	Q96AW1.
Genevestigator	Q96AW1.
Family and domain databases
ProtoNet	Search...
Other
NextBio	71799.

Entry information

Entry name

VOPP1_HUMAN

Accession

Primary (citable) accession number: Q96AW1
Secondary accession number(s): B2RAT4 , Q6FIE3, Q8NBN8, Q96RE5, Q9H0W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.