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Reviewed, UniProtKB/Swiss-Prot Q96AV8 (E2F7_HUMAN)

Last modified July 13, 2010. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Transcription factor E2F7

Short name=E2F-7
Gene names
Name:E2F7
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
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Protein attributesHide

Sequence length911 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription By similarity. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Ref.4 Ref.5

Subunit structure

Forms homodimers and, to a lesser extent, heterodimers with E2F8. Dimerization is important for DNA binding. Ref.6

Subcellular location

Nucleus Ref.5.

Domain

Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.

Sequence similarities

Belongs to the E2F/DP family.

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OntologiesHide

Keywords
   Biological processCell cycle
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandDNA-binding
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: InterPro

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componenttranscription factor complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionidentical protein binding Ref.5

Inferred from physical interaction. Source: IntAct

Complete GO annotation...
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Binary interactionsHide

With

Entry

#Exp.

IntAct

Notes

itself2EBI-1386765,EBI-1386765
RUVBL1Q9Y2651EBI-1386765,EBI-353675
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Alternative productsHide

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96AV8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96AV8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     714-845: GLNGFNVLLS...QQPESPVYVG → VTSSSDPQEHPTHTS
     846-911: Missing.
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 911911Transcription factor E2F7
PRO_0000298907

Regions

DNA binding142 – 21170 Potential
DNA binding282 – 36786 Potential

Amino acid modifications

Modified residue951Phosphoserine Ref.8
Modified residue4101Phosphoserine Ref.7

Natural variations

Alternative sequence714 – 845132GLNGF…PVYVG → VTSSSDPQEHPTHTS in isoform 2.
VSP_027473
Alternative sequence846 – 91166Missing in isoform 2.
VSP_027474
Natural variant721L → F. [dbSNP:rs310791] Ref.1 Ref.2
VAR_034732
Natural variant6261M → V. [dbSNP:rs3829295]
VAR_034733
Natural variant8541Q → H. [dbSNP:rs310831] Ref.1 Ref.2
VAR_034734

Experimental info

Mutagenesis147 – 1482LG → EE: Loss of DNA-binding and E2F-dependent repression. Ref.4
Mutagenesis1851R → A: Loss of DNA-binding and inhibition of E2F1-dependent activation. Ref.4 Ref.5
Mutagenesis3341R → A: Loss of DNA-binding and inhibition of E2F1-dependent activation. Ref.4 Ref.5
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SequencesHide

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 21, 2007. Version 2.
Checksum: 1B2FA12B1255E13F

FASTA91199,845
        10         20         30         40         50         60 
MEVNCLTLKD LISPRQPRLD FAVEDGENAQ KENIFVDRSR MAPKTPIKNE PIDLSKQKKF 

        70         80         90        100        110        120 
TPERNPITPV KLVDRQQAEP WTPTANLKML ISAASPDIRD REKKKGLFRP IENKDDAFTD 

       130        140        150        160        170        180 
SLQLDVVGDS AVDEFEKQRP SRKQKSLGLL CQKFLARYPS YPLSTEKTTI SLDEVAVSLG 

       190        200        210        220        230        240 
VERRRIYDIV NVLESLHLVS RVAKNQYGWH GRHSLPKTLR NLQRLGEEQK YEEQMAYLQQ 

       250        260        270        280        290        300 
KELDLIDYKF GERKKDGDPD SQEQQLLDFS EPDCPSSSAN SRKDKSLRIM SQKFVMLFLV 

       310        320        330        340        350        360 
SKTKIVTLDV AAKILIEESQ DAPDHSKFKT KVRRLYDIAN VLTSLALIKK VHVTEERGRK 

       370        380        390        400        410        420 
PAFKWIGPVD FSSSDEELVD VSASVLPELK RETYGQIQVC AKQKLARHGS FNTVQASERI 

       430        440        450        460        470        480 
QRKVNSEPSS PYREEQGSGG YSLEIGSLAA VYRQKIEDNS QGKAFASKRV VPPSSSLDPV 

       490        500        510        520        530        540 
APFPVLSVDP EYCVNPLAHP VFSVAQTDLQ AFSMQNGLNG QVDVSLASAA SAVESLKPAL 

       550        560        570        580        590        600 
LAGQPLVYVP SASLFMLYGS LQEGPASGSG SERDDRSSEA PATVELSSAP SAQKRLCEER 

       610        620        630        640        650        660 
KPQEEDEPAT KRQSREYEDG PLSLVMPKKP SDSTDLASPK TMGNRASIPL KDIHVNGQLP 

       670        680        690        700        710        720 
AAEEISGKAT ANSLVSSEWG NPSRNTDVEK PSKENESTKE PSLLQYLCVQ SPAGLNGFNV 

       730        740        750        760        770        780 
LLSGSQTPPT VGPSSGQLPS FSVPCMVLPS PPLGPFPVLY SPAMPGPVSS TLGALPNTGP 

       790        800        810        820        830        840 
VNFSLPGLGS IAQLLVGPTA VVNPKSSTLP SADPQLQSQP SLNLSPVMSR SHSVVQQPES 

       850        860        870        880        890        900 
PVYVGHPVSV VKLQQSPVPV TPKSIQRTHR ETFFKTPGSL GDPVLKRRER NQSRNTSSAQ 

       910 
RRLEIPSGGA D 

« Hide

Isoform 2.

Checksum: B5D8C35C9920F325
Show »

FASTA72880,832
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ReferencesHide

« Hide 'large scale' references
[1]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed: 16541075] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS PHE-72 AND HIS-854.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 595-911 (ISOFORM 2), VARIANTS PHE-72 AND HIS-854.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-911 (ISOFORM 1).
[4]"E2F7, a novel E2F featuring DP-independent repression of a subset of E2F-regulated genes."
Di Stefano L., Jensen M.R., Helin K.
EMBO J. 22:6289-6298(2003) [PubMed: 14633988] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, SELF-ASSOCIATION, MUTAGENESIS OF 147-LEU-GLY-148.
[5]"E2F-7: a distinctive E2F family member with an unusual organization of DNA-binding domains."
Logan N., Delavaine L., Graham A., Reilly C., Wilson J., Brummelkamp T.R., Hijmans E.M., Bernards R., La Thangue N.B.
Oncogene 23:5138-5150(2004) [PubMed: 15133492] [Abstract]
Cited for: FUNCTION, SELF-ASSOCIATION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-185 AND ARG-334.
[6]"Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development."
Li J., Ran C., Li E., Gordon F., Comstock G., Siddiqui H., Cleghorn W., Chen H.-Z., Kornacker K., Liu C.-G., Pandit S.K., Khanizadeh M., Weinstein M., Leone G., de Bruin A.
Dev. Cell 14:62-75(2008) [PubMed: 18194653] [Abstract]
Cited for: DIMERIZATION.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
+Additional computationally mapped references.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ
AC025161 Genomic DNA. No translation available.
AC079030 Genomic DNA. No translation available.
BC016658 mRNA. Translation: AAH16658.1.
BC136366 mRNA. Translation: AAI36367.1.
BC136367 mRNA. Translation: AAI36368.1.
BC017481 mRNA. No translation available.
AK097677 mRNA. No translation available.
IPIIPI00414604.
IPI00854868.
UniGeneHs.416375

3D structure databases

SMRQ96AV8. Positions 141-210, 281-365.
ModBaseSearch...

Protein-protein interaction databases

IntActQ96AV8. 2 interactions.
MINTMINT-6541592.
STRINGQ96AV8.

PTM databases

PhosphoSiteQ96AV8.

Proteomic databases

PRIDEQ96AV8.

Genome annotation databases

EnsemblENST00000322886; ENSP00000323246; ENSG00000165891; Homo sapiens. [Genome view]

Organism-specific databases

GeneCardsGC12M075917.
H-InvDBHIX0010843.
HGNCHGNC:23820. E2F7.
MIM612046. gene.
PharmGKBPA134894421.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG13390.
HOGENOMHBG505309.
HOVERGENHBG063270.
InParanoidQ96AV8.

Gene expression databases

ArrayExpressQ96AV8.
BgeeQ96AV8.
CleanExHS_E2F7.
GenevestigatorQ96AV8.

Family and domain databases

InterProIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
PANTHERPTHR12081. E2F. 1 hit.
PfamPF02319. E2F_TDP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...
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Entry informationHide

Entry nameE2F7_HUMAN
AccessionPrimary (citable) accession number: Q96AV8
Secondary accession number(s): A6NC74, B2RMR7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: August 21, 2007
Last modified: July 13, 2010
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.
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Relevant documentsHide

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents