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Protein

Ribulose-phosphate 3-epimerase

Gene

RPE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.1 Publication

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.1 Publication

Cofactori

Fe2+1 Publication, Mn2+1 Publication, Zn2+1 Publication, Co2+1 PublicationNote: Binds 1 divalent metal cation per subunit. Active with Fe2+, and probably also with Mn2+, Zn2+ and Co2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Substrate1
Metal bindingi35Divalent metal cation1
Active sitei37Proton acceptorCurated1
Metal bindingi37Divalent metal cation1
Metal bindingi70Divalent metal cation1
Binding sitei70Substrate1
Active sitei175Proton donorCurated1
Metal bindingi175Divalent metal cation1
Binding sitei177Substrate; via amide nitrogen1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • ribulose-phosphate 3-epimerase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS04202-MONOMER.
BRENDAi5.1.3.1. 2681.
ReactomeiR-HSA-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase (EC:5.1.3.1)
Alternative name(s):
Ribulose-5-phosphate-3-epimerase
Gene namesi
Name:RPE
ORF Names:HUSSY-17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:10293. RPE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi12L → A: Reduces enzyme activity by half. 1 Publication1
Mutagenesisi35H → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi37D → A: Alters protein structure. Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi39M → A: Lowers enzyme activity by 10%. 1 Publication1
Mutagenesisi70H → A: Alters protein structure. 1 Publication1
Mutagenesisi72M → A: Reduces enzyme activity by half. 1 Publication1
Mutagenesisi141M → A: No effect on enzyme activity. 1 Publication1
Mutagenesisi175D → A: Alters protein structure. 1 Publication1

Organism-specific databases

DisGeNETi6120.
OpenTargetsiENSG00000197713.
PharmGKBiPA34654.

Polymorphism and mutation databases

DMDMi34924986.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001715872 – 228Ribulose-phosphate 3-epimeraseAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ96AT9.
MaxQBiQ96AT9.
PaxDbiQ96AT9.
PeptideAtlasiQ96AT9.
PRIDEiQ96AT9.
TopDownProteomicsiQ96AT9-1. [Q96AT9-1]
Q96AT9-2. [Q96AT9-2]

PTM databases

iPTMnetiQ96AT9.
PhosphoSitePlusiQ96AT9.

Expressioni

Gene expression databases

BgeeiENSG00000197713.
CleanExiHS_RPE.
ExpressionAtlasiQ96AT9. baseline and differential.
GenevisibleiQ96AT9. HS.

Organism-specific databases

HPAiHPA036498.
HPA036499.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-372480,EBI-372480

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi112040. 50 interactors.
IntActiQ96AT9. 3 interactors.
MINTiMINT-1448309.
STRINGi9606.ENSP00000352401.

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi16 – 18Combined sources3
Helixi19 – 28Combined sources10
Beta strandi34 – 45Combined sources12
Helixi51 – 61Combined sources11
Beta strandi63 – 65Combined sources3
Beta strandi67 – 72Combined sources6
Helixi76 – 79Combined sources4
Helixi80 – 86Combined sources7
Beta strandi89 – 94Combined sources6
Helixi95 – 97Combined sources3
Helixi101 – 110Combined sources10
Beta strandi114 – 119Combined sources6
Helixi125 – 127Combined sources3
Helixi129 – 134Combined sources6
Beta strandi136 – 143Combined sources8
Turni145 – 147Combined sources3
Helixi154 – 156Combined sources3
Helixi157 – 166Combined sources10
Beta strandi171 – 177Combined sources7
Turni180 – 182Combined sources3
Helixi183 – 189Combined sources7
Beta strandi193 – 197Combined sources5
Helixi198 – 201Combined sources4
Helixi206 – 223Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OVPX-ray1.70A/B1-228[»]
3OVQX-ray2.00A/B1-228[»]
3OVRX-ray1.95A/B1-228[»]
3QC3X-ray2.20A/B1-224[»]
ProteinModelPortaliQ96AT9.
SMRiQ96AT9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96AT9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni146 – 149Substrate binding4
Regioni197 – 198Substrate binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3111. Eukaryota.
COG0036. LUCA.
GeneTreeiENSGT00390000001447.
HOGENOMiHOG000259349.
HOVERGENiHBG044821.
InParanoidiQ96AT9.
KOiK01783.
OMAiKTIDVCA.
OrthoDBiEOG091G0JMQ.
PhylomeDBiQ96AT9.
TreeFamiTF300157.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96AT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGCKIGPS ILNSDLANLG AECLRMLDSG ADYLHLDVMD GHFVPNITFG
60 70 80 90 100
HPVVESLRKQ LGQDPFFDMH MMVSKPEQWV KPMAVAGANQ YTFHLEATEN
110 120 130 140 150
PGALIKDIRE NGMKVGLAIK PGTSVEYLAP WANQIDMALV MTVEPGFGGQ
160 170 180 190 200
KFMEDMMPKV HWLRTQFPSL DIEVDGGVGP DTVHKCAEAG ANMIVSGSAI
210 220
MRSEDPRSVI NLLRNVCSEA AQKRSLDR
Length:228
Mass (Da):24,928
Last modified:December 1, 2001 - v1
Checksum:i447130018AC52331
GO
Isoform 2 (identifier: Q96AT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-68: Missing.
     114-114: K → KSCSVTQAEVQWHSQGPLQ

Note: No experimental confirmation available.
Show »
Length:220
Mass (Da):23,924
Checksum:i626FAFD9426AE406
GO
Isoform 3 (identifier: Q96AT9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.
     114-114: K → KSCSVTQAEVQWHSQGPLQ

Note: Gene prediction based on EST data.
Show »
Length:178
Mass (Da):19,537
Checksum:iC2D4353C7FFAEC41
GO
Isoform 4 (identifier: Q96AT9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-188: Missing.

Note: No experimental confirmation available.
Show »
Length:199
Mass (Da):21,739
Checksum:i88D6C3598F28A1E1
GO
Isoform 5 (identifier: Q96AT9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: Gene prediction based on EST data.
Show »
Length:160
Mass (Da):17,570
Checksum:i9B6890E5E78B7D35
GO

Sequence cautioni

The sequence BAB71076 differs from that shown. Reason: Frameshift at position 69.Curated
The sequence BAC04212 differs from that shown. Reason: Frameshift at position 42.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180P → L in BAC04212 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0471171 – 68Missing in isoform 3 and isoform 5. CuratedAdd BLAST68
Alternative sequenceiVSP_00831743 – 68Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_008318114K → KSCSVTQAEVQWHSQGPLQ in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_055265160 – 188Missing in isoform 4. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056028 mRNA. Translation: BAB71076.1. Frameshift.
AK093658 mRNA. Translation: BAC04212.1. Frameshift.
AK291035 mRNA. Translation: BAF83724.1.
AK303184 mRNA. Translation: BAG64278.1.
AC007038 Genomic DNA. Translation: AAX93087.1.
CH471063 Genomic DNA. Translation: EAW70473.1.
CH471063 Genomic DNA. Translation: EAW70474.1.
BC005148 mRNA. Translation: AAH05148.2.
BC016764 mRNA. Translation: AAH16764.1.
BC072401 mRNA. Translation: AAH72401.1.
AJ224326 mRNA. Translation: CAA11895.1.
CCDSiCCDS2388.1. [Q96AT9-1]
CCDS42810.1. [Q96AT9-3]
CCDS63107.1. [Q96AT9-4]
CCDS63108.1. [Q96AT9-5]
RefSeqiNP_001265211.1. NM_001278282.1. [Q96AT9-3]
NP_001265212.1. NM_001278283.1. [Q96AT9-3]
NP_001265214.1. NM_001278285.1. [Q96AT9-4]
NP_001265215.1. NM_001278286.1. [Q96AT9-5]
NP_001265217.1. NM_001278288.1. [Q96AT9-5]
NP_001265218.1. NM_001278289.1.
NP_001305855.1. NM_001318926.1.
NP_001305856.1. NM_001318927.1.
NP_001305857.1. NM_001318928.1.
NP_001305858.1. NM_001318929.1.
NP_001305859.1. NM_001318930.1. [Q96AT9-5]
NP_001305860.1. NM_001318931.1. [Q96AT9-5]
NP_008847.1. NM_006916.2. [Q96AT9-3]
NP_954699.1. NM_199229.2. [Q96AT9-1]
XP_006712740.1. XM_006712677.3. [Q96AT9-5]
UniGeneiHs.282260.
Hs.591638.
Hs.734255.

Genome annotation databases

EnsembliENST00000354506; ENSP00000346501; ENSG00000197713. [Q96AT9-4]
ENST00000359429; ENSP00000352401; ENSG00000197713. [Q96AT9-1]
ENST00000411934; ENSP00000389411; ENSG00000197713. [Q96AT9-5]
ENST00000429921; ENSP00000401838; ENSG00000197713. [Q96AT9-3]
ENST00000436630; ENSP00000403808; ENSG00000197713. [Q96AT9-3]
ENST00000438204; ENSP00000402061; ENSG00000197713. [Q96AT9-5]
ENST00000454822; ENSP00000394455; ENSG00000197713. [Q96AT9-3]
GeneIDi6120.
KEGGihsa:6120.
UCSCiuc002vdn.5. human. [Q96AT9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056028 mRNA. Translation: BAB71076.1. Frameshift.
AK093658 mRNA. Translation: BAC04212.1. Frameshift.
AK291035 mRNA. Translation: BAF83724.1.
AK303184 mRNA. Translation: BAG64278.1.
AC007038 Genomic DNA. Translation: AAX93087.1.
CH471063 Genomic DNA. Translation: EAW70473.1.
CH471063 Genomic DNA. Translation: EAW70474.1.
BC005148 mRNA. Translation: AAH05148.2.
BC016764 mRNA. Translation: AAH16764.1.
BC072401 mRNA. Translation: AAH72401.1.
AJ224326 mRNA. Translation: CAA11895.1.
CCDSiCCDS2388.1. [Q96AT9-1]
CCDS42810.1. [Q96AT9-3]
CCDS63107.1. [Q96AT9-4]
CCDS63108.1. [Q96AT9-5]
RefSeqiNP_001265211.1. NM_001278282.1. [Q96AT9-3]
NP_001265212.1. NM_001278283.1. [Q96AT9-3]
NP_001265214.1. NM_001278285.1. [Q96AT9-4]
NP_001265215.1. NM_001278286.1. [Q96AT9-5]
NP_001265217.1. NM_001278288.1. [Q96AT9-5]
NP_001265218.1. NM_001278289.1.
NP_001305855.1. NM_001318926.1.
NP_001305856.1. NM_001318927.1.
NP_001305857.1. NM_001318928.1.
NP_001305858.1. NM_001318929.1.
NP_001305859.1. NM_001318930.1. [Q96AT9-5]
NP_001305860.1. NM_001318931.1. [Q96AT9-5]
NP_008847.1. NM_006916.2. [Q96AT9-3]
NP_954699.1. NM_199229.2. [Q96AT9-1]
XP_006712740.1. XM_006712677.3. [Q96AT9-5]
UniGeneiHs.282260.
Hs.591638.
Hs.734255.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OVPX-ray1.70A/B1-228[»]
3OVQX-ray2.00A/B1-228[»]
3OVRX-ray1.95A/B1-228[»]
3QC3X-ray2.20A/B1-224[»]
ProteinModelPortaliQ96AT9.
SMRiQ96AT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112040. 50 interactors.
IntActiQ96AT9. 3 interactors.
MINTiMINT-1448309.
STRINGi9606.ENSP00000352401.

PTM databases

iPTMnetiQ96AT9.
PhosphoSitePlusiQ96AT9.

Polymorphism and mutation databases

DMDMi34924986.

Proteomic databases

EPDiQ96AT9.
MaxQBiQ96AT9.
PaxDbiQ96AT9.
PeptideAtlasiQ96AT9.
PRIDEiQ96AT9.
TopDownProteomicsiQ96AT9-1. [Q96AT9-1]
Q96AT9-2. [Q96AT9-2]

Protocols and materials databases

DNASUi6120.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354506; ENSP00000346501; ENSG00000197713. [Q96AT9-4]
ENST00000359429; ENSP00000352401; ENSG00000197713. [Q96AT9-1]
ENST00000411934; ENSP00000389411; ENSG00000197713. [Q96AT9-5]
ENST00000429921; ENSP00000401838; ENSG00000197713. [Q96AT9-3]
ENST00000436630; ENSP00000403808; ENSG00000197713. [Q96AT9-3]
ENST00000438204; ENSP00000402061; ENSG00000197713. [Q96AT9-5]
ENST00000454822; ENSP00000394455; ENSG00000197713. [Q96AT9-3]
GeneIDi6120.
KEGGihsa:6120.
UCSCiuc002vdn.5. human. [Q96AT9-1]

Organism-specific databases

CTDi6120.
DisGeNETi6120.
GeneCardsiRPE.
H-InvDBHIX0190555.
HGNCiHGNC:10293. RPE.
HPAiHPA036498.
HPA036499.
MIMi180480. gene.
neXtProtiNX_Q96AT9.
OpenTargetsiENSG00000197713.
PharmGKBiPA34654.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3111. Eukaryota.
COG0036. LUCA.
GeneTreeiENSGT00390000001447.
HOGENOMiHOG000259349.
HOVERGENiHBG044821.
InParanoidiQ96AT9.
KOiK01783.
OMAiKTIDVCA.
OrthoDBiEOG091G0JMQ.
PhylomeDBiQ96AT9.
TreeFamiTF300157.

Enzyme and pathway databases

BioCyciZFISH:HS04202-MONOMER.
BRENDAi5.1.3.1. 2681.
ReactomeiR-HSA-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

EvolutionaryTraceiQ96AT9.
GeneWikiiRPE_(gene).
GenomeRNAii6120.
PROiQ96AT9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197713.
CleanExiHS_RPE.
ExpressionAtlasiQ96AT9. baseline and differential.
GenevisibleiQ96AT9. HS.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPE_HUMAN
AccessioniPrimary (citable) accession number: Q96AT9
Secondary accession number(s): A8K4S0
, B4E016, C9JPQ7, O43767, Q53TV9, Q8N215, Q96N34, Q9BSB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.