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Q96AJ9 (VTI1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2
Gene names
Name:VTI1A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Ref.5 Ref.6 UniProtKB O89116

Subunit structure

Interacts with distinct SNARE complexes that contain either STX5 or STX6 By similarity. Interacts with NAPA and, to a lesser extent, with NAPG By similarity. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A By similarity.

Subcellular location

Cytoplasmic vesicle. Golgi apparatus membrane; Single-pass type IV membrane protein By similarity Ref.6.

Sequence similarities

Belongs to the VTI1 family. UniProtKB O89116

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q96AJ9-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q96AJ9-1)

The sequence of this isoform differs from the canonical sequence as follows:
     188-217: IIQNRILLVILGIIVVITILMAITFSVRRH → GCSVKKQCNLSLAPKA
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217Vesicle transport through interaction with t-SNAREs homolog 1A
PRO_0000218225

Regions

Topological domain1 – 192192Cytoplasmic Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 2174Extracellular Potential
Coiled coil31 – 9262 Potential
Coiled coil112 – 17867 Potential

Natural variations

Alternative sequence188 – 21730IIQNR…SVRRH → GCSVKKQCNLSLAPKA in isoform 1.
VSP_039848

Experimental info

Sequence conflict831K → E in BAG64649. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified January 25, 2012. Version 2.
Checksum: 3FE5FE787B5958F0

FASTA21725,218
        10         20         30         40         50         60 
MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEAKE LLEQMDLEVR 

        70         80         90        100        110        120 
EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDDGNS SENQRAHLLD 

       130        140        150        160        170        180 
NTERLERSSR RLEAGYQIAV ETEQIGQEML ENLSHDREKI QRARERLRET DANLGKSSRI 

       190        200        210 
LTGMLRRIIQ NRILLVILGI IVVITILMAI TFSVRRH 

« Hide

Isoform 1 [UniParc].

Checksum: 751DB052B552BC09
Show »

FASTA20323,435

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Thymus.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[5]"A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells."
Ganley I.G., Espinosa E., Pfeffer S.R.
J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels."
Flowerdew S.E., Burgoyne R.D.
Biochem. J. 418:529-540(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK303646 mRNA. Translation: BAG64649.1.
AL139120, AC022018, AL158212 Genomic DNA. Translation: CAH71781.2.
AL139120, AC022018 Genomic DNA. Translation: CAM15142.1.
AL158212, AC022018, AL139120 Genomic DNA. Translation: CAM22437.1.
CH471066 Genomic DNA. Translation: EAW49524.1.
CH471066 Genomic DNA. Translation: EAW49525.1.
BC017052 mRNA. Translation: AAH17052.1.
CCDSCCDS7575.2. [Q96AJ9-2]
RefSeqNP_660207.2. NM_145206.2. [Q96AJ9-2]
XP_005269601.1. XM_005269544.2. [Q96AJ9-1]
UniGeneHs.194554.

3D structure databases

ProteinModelPortalQ96AJ9.
SMRQ96AJ9. Positions 8-94, 113-190.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126789. 8 interactions.
DIPDIP-44223N.
IntActQ96AJ9. 3 interactions.
MINTMINT-3050814.
STRING9606.ENSP00000376792.

PTM databases

PhosphoSiteQ96AJ9.

Polymorphism databases

DMDM374095459.

Proteomic databases

MaxQBQ96AJ9.
PaxDbQ96AJ9.
PeptideAtlasQ96AJ9.
PRIDEQ96AJ9.

Protocols and materials databases

DNASU143187.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000393077; ENSP00000376792; ENSG00000151532. [Q96AJ9-2]
ENST00000432306; ENSP00000395017; ENSG00000151532. [Q96AJ9-1]
GeneID143187.
KEGGhsa:143187.
UCSCuc001kzy.3. human. [Q96AJ9-1]
uc001kzz.3. human. [Q96AJ9-2]

Organism-specific databases

CTD143187.
GeneCardsGC10P114197.
H-InvDBHIX0023094.
HGNCHGNC:17792. VTI1A.
HPAHPA054108.
MIM614316. gene.
neXtProtNX_Q96AJ9.
PharmGKBPA134928049.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG239679.
HOGENOMHOG000116573.
HOVERGENHBG104027.
KOK08493.
OMAADFKRSR.
OrthoDBEOG7MKW77.
PhylomeDBQ96AJ9.
TreeFamTF312874.

Gene expression databases

BgeeQ96AJ9.
CleanExHS_VTI1A.
GenevestigatorQ96AJ9.

Family and domain databases

InterProIPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamPF05008. V-SNARE. 1 hit.
[Graphical view]
SMARTSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMSSF47661. SSF47661. 1 hit.
ProtoNetSearch...

Other

GeneWikiVTI1A.
GenomeRNAi143187.
NextBio84666.
PROQ96AJ9.
SOURCESearch...

Entry information

Entry nameVTI1A_HUMAN
AccessionPrimary (citable) accession number: Q96AJ9
Secondary accession number(s): A2A307, B4E137, Q5W0D7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 25, 2012
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM