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Protein

Vesicle transport through interaction with t-SNAREs homolog 1A

Gene

VTI1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence.2 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: HGNC

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: ParkinsonsUK-UCL
  • Golgi ribbon formation Source: UniProtKB
  • intracellular protein transport Source: InterPro
  • retrograde transport, endosome to Golgi Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: ParkinsonsUK-UCL
  • voluntary musculoskeletal movement Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2
Gene namesi
Name:VTI1A
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:17792. VTI1A.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 192192CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Topological domaini214 – 2174ExtracellularSequence Analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134928049.

Polymorphism and mutation databases

BioMutaiVTI1A.
DMDMi374095459.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Vesicle transport through interaction with t-SNAREs homolog 1APRO_0000218225Add
BLAST

Proteomic databases

MaxQBiQ96AJ9.
PaxDbiQ96AJ9.
PeptideAtlasiQ96AJ9.
PRIDEiQ96AJ9.

PTM databases

PhosphoSiteiQ96AJ9.

Expressioni

Gene expression databases

BgeeiQ96AJ9.
CleanExiHS_VTI1A.
GenevisibleiQ96AJ9. HS.

Organism-specific databases

HPAiHPA054108.

Interactioni

Subunit structurei

Interacts with distinct SNARE complexes that contain either STX5 or STX6. Interacts with NAPA and, to a lesser extent, with NAPG. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.By similarity

Protein-protein interaction databases

BioGridi126789. 12 interactions.
DIPiDIP-44223N.
IntActiQ96AJ9. 3 interactions.
MINTiMINT-3050814.
STRINGi9606.ENSP00000376792.

Structurei

3D structure databases

ProteinModelPortaliQ96AJ9.
SMRiQ96AJ9. Positions 8-94, 113-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili31 – 9262Sequence AnalysisAdd
BLAST
Coiled coili112 – 17867Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239679.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiQ96AJ9.
KOiK08493.
OMAiLIANTQR.
OrthoDBiEOG7MKW77.
PhylomeDBiQ96AJ9.
TreeFamiTF312874.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q96AJ9-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEAKE
60 70 80 90 100
LLEQMDLEVR EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR
110 120 130 140 150
NELLGDDGNS SENQRAHLLD NTERLERSSR RLEAGYQIAV ETEQIGQEML
160 170 180 190 200
ENLSHDREKI QRARERLRET DANLGKSSRI LTGMLRRIIQ NRILLVILGI
210
IVVITILMAI TFSVRRH
Length:217
Mass (Da):25,218
Last modified:January 25, 2012 - v2
Checksum:i3FE5FE787B5958F0
GO
Isoform 1 (identifier: Q96AJ9-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-217: IIQNRILLVILGIIVVITILMAITFSVRRH → GCSVKKQCNLSLAPKA

Note: No experimental confirmation available.
Show »
Length:203
Mass (Da):23,435
Checksum:i751DB052B552BC09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831K → E in BAG64649 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei188 – 21730IIQNR…SVRRH → GCSVKKQCNLSLAPKA in isoform 1. 1 PublicationVSP_039848Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK303646 mRNA. Translation: BAG64649.1.
AL139120, AC022018, AL158212 Genomic DNA. Translation: CAH71781.2.
AL139120, AC022018 Genomic DNA. Translation: CAM15142.1.
AL158212, AC022018, AL139120 Genomic DNA. Translation: CAM22437.1.
CH471066 Genomic DNA. Translation: EAW49524.1.
CH471066 Genomic DNA. Translation: EAW49525.1.
BC017052 mRNA. Translation: AAH17052.1.
CCDSiCCDS7575.2. [Q96AJ9-2]
RefSeqiNP_660207.2. NM_145206.2. [Q96AJ9-2]
XP_005269601.1. XM_005269544.3. [Q96AJ9-1]
UniGeneiHs.194554.

Genome annotation databases

EnsembliENST00000393077; ENSP00000376792; ENSG00000151532. [Q96AJ9-2]
ENST00000432306; ENSP00000395017; ENSG00000151532. [Q96AJ9-1]
GeneIDi143187.
KEGGihsa:143187.
UCSCiuc001kzy.3. human. [Q96AJ9-1]
uc001kzz.3. human. [Q96AJ9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK303646 mRNA. Translation: BAG64649.1.
AL139120, AC022018, AL158212 Genomic DNA. Translation: CAH71781.2.
AL139120, AC022018 Genomic DNA. Translation: CAM15142.1.
AL158212, AC022018, AL139120 Genomic DNA. Translation: CAM22437.1.
CH471066 Genomic DNA. Translation: EAW49524.1.
CH471066 Genomic DNA. Translation: EAW49525.1.
BC017052 mRNA. Translation: AAH17052.1.
CCDSiCCDS7575.2. [Q96AJ9-2]
RefSeqiNP_660207.2. NM_145206.2. [Q96AJ9-2]
XP_005269601.1. XM_005269544.3. [Q96AJ9-1]
UniGeneiHs.194554.

3D structure databases

ProteinModelPortaliQ96AJ9.
SMRiQ96AJ9. Positions 8-94, 113-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126789. 12 interactions.
DIPiDIP-44223N.
IntActiQ96AJ9. 3 interactions.
MINTiMINT-3050814.
STRINGi9606.ENSP00000376792.

PTM databases

PhosphoSiteiQ96AJ9.

Polymorphism and mutation databases

BioMutaiVTI1A.
DMDMi374095459.

Proteomic databases

MaxQBiQ96AJ9.
PaxDbiQ96AJ9.
PeptideAtlasiQ96AJ9.
PRIDEiQ96AJ9.

Protocols and materials databases

DNASUi143187.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393077; ENSP00000376792; ENSG00000151532. [Q96AJ9-2]
ENST00000432306; ENSP00000395017; ENSG00000151532. [Q96AJ9-1]
GeneIDi143187.
KEGGihsa:143187.
UCSCiuc001kzy.3. human. [Q96AJ9-1]
uc001kzz.3. human. [Q96AJ9-2]

Organism-specific databases

CTDi143187.
GeneCardsiGC10P114197.
H-InvDBHIX0023094.
HGNCiHGNC:17792. VTI1A.
HPAiHPA054108.
MIMi614316. gene.
neXtProtiNX_Q96AJ9.
PharmGKBiPA134928049.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG239679.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiQ96AJ9.
KOiK08493.
OMAiLIANTQR.
OrthoDBiEOG7MKW77.
PhylomeDBiQ96AJ9.
TreeFamiTF312874.

Miscellaneous databases

ChiTaRSiVTI1A. human.
GeneWikiiVTI1A.
GenomeRNAii143187.
NextBioi84666.
PROiQ96AJ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ96AJ9.
CleanExiHS_VTI1A.
GenevisibleiQ96AJ9. HS.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Thymus.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells."
    Ganley I.G., Espinosa E., Pfeffer S.R.
    J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels."
    Flowerdew S.E., Burgoyne R.D.
    Biochem. J. 418:529-540(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVTI1A_HUMAN
AccessioniPrimary (citable) accession number: Q96AJ9
Secondary accession number(s): A2A307, B4E137, Q5W0D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 25, 2012
Last modified: June 24, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.