Q96AJ9 (VTI1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A

Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2

Gene names

Name:

VTI1A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Ref.5 Ref.6 UniProtKB O89116
Subunit structure	Interacts with distinct SNARE complexes that contain either STX5 or STX6 By similarity. Interacts with NAPA and, to a lesser extent, with NAPG By similarity. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A By similarity.
Subcellular location	Cytoplasmic vesicle. Golgi apparatus membrane; Single-pass type IV membrane protein By similarity Ref.6.
Sequence similarities	Belongs to the VTI1 family. UniProtKB O89116

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, endosome to Golgi Inferred from direct assay Ref.5. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 19224922. Source: UniProtKB Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Traceable author statement PubMed 15215310. Source: HGNC cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	SNAP receptor activity Inferred from direct assay PubMed 15215310. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q96AJ9-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q96AJ9-1)

The sequence of this isoform differs from the canonical sequence as follows:
     188-217: IIQNRILLVILGIIVVITILMAITFSVRRH → GCSVKKQCNLSLAPKA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Vesicle transport through interaction with t-SNAREs homolog 1A		PRO_0000218225
Regions
	Topological domain	1 – 192	192	Cytoplasmic Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Topological domain	214 – 217	4	Extracellular Potential
	Coiled coil	31 – 92	62	Potential
	Coiled coil	112 – 178	67	Potential
Natural variations
	Alternative sequence	188 – 217	30	IIQNR…SVRRH → GCSVKKQCNLSLAPKA in isoform 1.		VSP_039848
Experimental info
	Sequence conflict	83	1	K → E in BAG64649. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified January 25, 2012. Version 2. Checksum: 3FE5FE787B5958F0	FASTA	217	25,218
	10 20 30 40 50 60 MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEAKE LLEQMDLEVR 70 80 90 100 110 120 EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDDGNS SENQRAHLLD 130 140 150 160 170 180 NTERLERSSR RLEAGYQIAV ETEQIGQEML ENLSHDREKI QRARERLRET DANLGKSSRI 190 200 210 LTGMLRRIIQ NRILLVILGI IVVITILMAI TFSVRRH
	Isoform 1 [UniParc]. Checksum: 751DB052B552BC09	FASTA	203	23,435
	10 20 30 40 50 60 MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEAKE LLEQMDLEVR 70 80 90 100 110 120 EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDDGNS SENQRAHLLD 130 140 150 160 170 180 NTERLERSSR RLEAGYQIAV ETEQIGQEML ENLSHDREKI QRARERLRET DANLGKSSRI 190 200 LTGMLRRGCS VKKQCNLSLA PKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[5]	"A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells." Ganley I.G., Espinosa E., Pfeffer S.R. J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels." Flowerdew S.E., Burgoyne R.D. Biochem. J. 418:529-540(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK303646 mRNA. Translation: BAG64649.1. AL139120, AC022018, AL158212 Genomic DNA. Translation: CAH71781.2. AL139120, AC022018 Genomic DNA. Translation: CAM15142.1. AL158212, AC022018, AL139120 Genomic DNA. Translation: CAM22437.1. CH471066 Genomic DNA. Translation: EAW49524.1. CH471066 Genomic DNA. Translation: EAW49525.1. BC017052 mRNA. Translation: AAH17052.1.
IPI	IPI00059472. IPI00852968.
RefSeq	NP_660207.2. NM_145206.2.
UniGene	Hs.194554.
3D structure databases
ProteinModelPortal	Q96AJ9.
SMR	Q96AJ9. Positions 8-94, 113-190.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96AJ9. 2 interactions.
MINT	MINT-3050814.
STRING	9606.ENSP00000376792.
PTM databases
PhosphoSite	Q96AJ9.
Polymorphism databases
DMDM	29337113.
Proteomic databases
PaxDb	Q96AJ9.
PeptideAtlas	Q96AJ9.
PRIDE	Q96AJ9.
Protocols and materials databases
DNASU	143187.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000393077; ENSP00000376792; ENSG00000151532. ENST00000432306; ENSP00000395017; ENSG00000151532.
GeneID	143187.
KEGG	hsa:143187.
UCSC	uc001kzy.3. human.
Organism-specific databases
CTD	143187.
GeneCards	GC10P114197.
H-InvDB	HIX0023094.
HGNC	HGNC:17792. VTI1A.
HPA	HPA054108.
MIM	614316. gene.
neXtProt	NX_Q96AJ9.
PharmGKB	PA134928049.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG239679.
HOGENOM	HOG000116573.
HOVERGEN	HBG104027.
KO	K08493.
OMA	YEQDFGT.
OrthoDB	EOG4V6ZHV.
Gene expression databases
ArrayExpress	Q96AJ9.
Bgee	Q96AJ9.
CleanEx	HS_VTI1A.
Genevestigator	Q96AJ9.
GermOnline	ENSG00000151532. Homo sapiens.
Family and domain databases
InterPro	IPR010989. t-SNARE. IPR000727. T_SNARE_dom. IPR007705. Vesicle_trsprt_v-SNARE_N. [Graphical view]
Pfam	PF05008. V-SNARE. 1 hit. [Graphical view]
SMART	SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	143187.
NextBio	84666.
SOURCE	Search...

Entry information

Entry name

VTI1A_HUMAN

Accession

Primary (citable) accession number: Q96AJ9
Secondary accession number(s): A2A307, B4E137, Q5W0D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 25, 2012
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.