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Protein

Clusterin-associated protein 1

Gene

CLUAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cilia biogenesis. Appears to function within the multiple intraflagellar transport complex B (IFT-B). Key regulator of hedgehog signaling (By similarity).By similarity

GO - Biological processi

  1. cilium assembly Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Clusterin-associated protein 1
Alternative name(s):
Qilin
Gene namesi
Name:CLUAP1
Synonyms:KIAA0643
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:19009. CLUAP1.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: GO_Central
  2. cilium Source: GO_Central
  3. intracellular membrane-bounded organelle Source: HPA
  4. intraciliary transport particle B Source: GO_Central
  5. nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394600.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Clusterin-associated protein 1PRO_0000239451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei409 – 4091Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96AJ1.
PaxDbiQ96AJ1.
PRIDEiQ96AJ1.

PTM databases

PhosphoSiteiQ96AJ1.

Expressioni

Tissue specificityi

Expressed in testis, thyroid and trachea and to a lower extent in spinal cord and adrenal gland. Highly expressed in colon cancer and osteosarcoma cell lines.2 Publications

Gene expression databases

BgeeiQ96AJ1.
CleanExiHS_CLUAP1.
ExpressionAtlasiQ96AJ1. baseline and differential.
GenevestigatoriQ96AJ1.

Organism-specific databases

HPAiHPA036976.
HPA044191.

Interactioni

Subunit structurei

Interacts with CLU/clusterin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAGEA11P433642EBI-739780,EBI-739552

Protein-protein interaction databases

BioGridi116694. 9 interactions.
IntActiQ96AJ1. 2 interactions.
STRINGi9606.ENSP00000344392.

Structurei

3D structure databases

ProteinModelPortaliQ96AJ1.
SMRiQ96AJ1. Positions 8-46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili198 – 29194Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi360 – 38627Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the CLUAP1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG330295.
GeneTreeiENSGT00390000008957.
HOGENOMiHOG000257815.
HOVERGENiHBG081253.
InParanoidiQ96AJ1.
OrthoDBiEOG7XDBG7.
PhylomeDBiQ96AJ1.
TreeFamiTF314639.

Family and domain databases

InterProiIPR019366. Clusterin-associated_protein-1.
[Graphical view]
PANTHERiPTHR21547. PTHR21547. 1 hit.
PfamiPF10234. Cluap1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q96AJ1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFRDLRNFT EMMRALGYPR HISMENFRTP NFGLVSEVLL WLVKRYEPQT
60 70 80 90 100
DIPPDVDTEQ DRVFFIKAIA QFMATKAHIK LNTKKLYQAD GYAVKELLKI
110 120 130 140 150
TSVLYNAMKT KGMEGSEIVE EDVNKFKFDL GSKIADLKAA RQLASEITSK
160 170 180 190 200
GASLYDLLGM EVELREMRTE AIARPLEINE TEKVMRIAIK EILTQVQKTK
210 220 230 240 250
DLLNNVASDE ANLEAKIEKR KLELERNRKR LETLQSVRPC FMDEYEKTEE
260 270 280 290 300
ELQKQYDTYL EKFQNLTYLE QQLEDHHRME QERFEEAKNT LCLIQNKLKE
310 320 330 340 350
EEKRLLKSGS NDDSDIDIQE DDESDSELEE RRLPKPQTAM EMLMQGRPGK
360 370 380 390 400
RIVGTMQGGD SDDNEDSEES EIDMEDDDDE DDDLEDESIS LSPTKPNRRV
410
RKSEPLDESD NDF
Length:413
Mass (Da):48,125
Last modified:June 13, 2006 - v4
Checksum:i7A23D4FA2EBE5A9F
GO
Isoform 2 (identifier: Q96AJ1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-166: Missing.

Note: No experimental confirmation available.

Show »
Length:247
Mass (Da):29,093
Checksum:i04DEC4E12F4D36F3
GO

Sequence cautioni

The sequence BAA31618.1 differs from that shown.Intron retention.Curated
The sequence BAB14523.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681A → S.
Corresponds to variant rs34115694 [ dbSNP | Ensembl ].
VAR_050869
Natural varianti401 – 4011R → W.1 Publication
Corresponds to variant rs9790 [ dbSNP | Ensembl ].
VAR_050870

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 166166Missing in isoform 2. 1 PublicationVSP_019229Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089691 mRNA. Translation: BAD44779.1.
AB014543 mRNA. Translation: BAA31618.1. Sequence problems.
AK023319 mRNA. Translation: BAB14523.1. Different initiation.
AK023359 mRNA. Translation: BAB14542.1.
BC017070 mRNA. Translation: AAH17070.3.
CCDSiCCDS32381.1. [Q96AJ1-1]
CCDS45398.1. [Q96AJ1-2]
RefSeqiNP_055856.1. NM_015041.2. [Q96AJ1-1]
NP_079069.1. NM_024793.2. [Q96AJ1-2]
UniGeneiHs.155995.

Genome annotation databases

EnsembliENST00000572600; ENSP00000460889; ENSG00000103351. [Q96AJ1-2]
ENST00000576634; ENSP00000460850; ENSG00000103351. [Q96AJ1-1]
GeneIDi23059.
KEGGihsa:23059.
UCSCiuc002cvk.2. human. [Q96AJ1-1]

Polymorphism databases

DMDMi108935970.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089691 mRNA. Translation: BAD44779.1.
AB014543 mRNA. Translation: BAA31618.1. Sequence problems.
AK023319 mRNA. Translation: BAB14523.1. Different initiation.
AK023359 mRNA. Translation: BAB14542.1.
BC017070 mRNA. Translation: AAH17070.3.
CCDSiCCDS32381.1. [Q96AJ1-1]
CCDS45398.1. [Q96AJ1-2]
RefSeqiNP_055856.1. NM_015041.2. [Q96AJ1-1]
NP_079069.1. NM_024793.2. [Q96AJ1-2]
UniGeneiHs.155995.

3D structure databases

ProteinModelPortaliQ96AJ1.
SMRiQ96AJ1. Positions 8-46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116694. 9 interactions.
IntActiQ96AJ1. 2 interactions.
STRINGi9606.ENSP00000344392.

PTM databases

PhosphoSiteiQ96AJ1.

Polymorphism databases

DMDMi108935970.

Proteomic databases

MaxQBiQ96AJ1.
PaxDbiQ96AJ1.
PRIDEiQ96AJ1.

Protocols and materials databases

DNASUi23059.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000572600; ENSP00000460889; ENSG00000103351. [Q96AJ1-2]
ENST00000576634; ENSP00000460850; ENSG00000103351. [Q96AJ1-1]
GeneIDi23059.
KEGGihsa:23059.
UCSCiuc002cvk.2. human. [Q96AJ1-1]

Organism-specific databases

CTDi23059.
GeneCardsiGC16P003550.
HGNCiHGNC:19009. CLUAP1.
HPAiHPA036976.
HPA044191.
neXtProtiNX_Q96AJ1.
PharmGKBiPA128394600.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG330295.
GeneTreeiENSGT00390000008957.
HOGENOMiHOG000257815.
HOVERGENiHBG081253.
InParanoidiQ96AJ1.
OrthoDBiEOG7XDBG7.
PhylomeDBiQ96AJ1.
TreeFamiTF314639.

Miscellaneous databases

GeneWikiiCLUAP1.
GenomeRNAii23059.
NextBioi44121.
PROiQ96AJ1.

Gene expression databases

BgeeiQ96AJ1.
CleanExiHS_CLUAP1.
ExpressionAtlasiQ96AJ1. baseline and differential.
GenevestigatoriQ96AJ1.

Family and domain databases

InterProiIPR019366. Clusterin-associated_protein-1.
[Graphical view]
PANTHERiPTHR21547. PTHR21547. 1 hit.
PfamiPF10234. Cluap1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer."
    Takahashi M., Lin Y.-M., Nakamura Y., Furukawa Y.
    Oncogene 23:9289-9294(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CLU, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Colon tumor.
  2. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 222-413 (ISOFORMS 1/2).
    Tissue: Ovary.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TRP-401.
    Tissue: Skin.
  5. "Human cilia proteome contains homolog of zebrafish polycystic kidney disease gene qilin."
    Marshall W.F.
    Curr. Biol. 14:R913-R914(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBCELLULAR LOCATION.
  6. "Identification of CLUAP1 as a human osteosarcoma tumor-associated antigen recognized by the humoral immune system."
    Ishikura H., Ikeda H., Abe H., Ohkuri T., Hiraga H., Isu K., Tsukahara T., Sato N., Kitamura H., Iwasaki N., Takeda N., Minami A., Nishimura T.
    Int. J. Oncol. 30:461-467(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiCLUA1_HUMAN
AccessioniPrimary (citable) accession number: Q96AJ1
Secondary accession number(s): O75138
, Q65ZA3, Q9H8R4, Q9H8T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: February 4, 2015
This is version 97 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Associated with a number of cancers such as colon (PubMed:15480429) and bone cancer (PubMed:17203229). Possibly involved in polycystic kidney diseases (PubMed:15530380).3 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.