Q96AH0 (SOSB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SOSS complex subunit B2

Alternative name(s):
Oligonucleotide/oligosaccharide-binding fold-containing protein 2A
Sensor of single-strand DNA complex subunit B2
Sensor of ssDNA subunit B2
Short name=SOSS-B2
Single-stranded DNA-binding protein 2
Short name=hSSB2

Gene names

Name:	OBFC2A
Synonyms:	SSB2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. Ref.6 Ref.7
Subunit structure	Component of the SOSS complex, composed of SOSS-B (SOSS-B1/OBFC2B or SOSS-B2/OBFC2A), SOSS-A/INTS3 and SOSS-C/C9orf80. SOSS complexes containing SOSS-B1/OBFC2B are more abundant than complexes containing SOSS-B2/OBFC2A.
Subcellular location	Nucleus. Note: Localizes to nuclear foci following DNA damage. Ref.6 Ref.7
Sequence similarities	Belongs to the SOSS-B family. SOSS-B2 subfamily. Contains 1 OB DNA-binding domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition checkpoint Inferred from mutant phenotype Ref.7. Source: UniProtKB double-strand break repair via homologous recombination Inferred from mutant phenotype Ref.6. Source: UniProtKB response to ionizing radiation Inferred from mutant phenotype Ref.6Ref.7. Source: UniProtKB
Cellular component	SOSS complex Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	single-stranded DNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96AH0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96AH0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: Missing.

Isoform 3 (identifier: Q96AH0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     101-134: EFCMVYSEVPNFSEPNPDYRGQQNKGAQSEQKNN → DLGAVQAAAMRDSIHYYPGNDLHPDLEEPSSLGV
     135-204: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	SOSS complex subunit B2		PRO_0000333954
Regions
	DNA binding	26 – 99	74	OB
Natural variations
	Alternative sequence	1 – 80	80	Missing in isoform 2.		VSP_033600
	Alternative sequence	101 – 134	34	EFCMV…EQKNN → DLGAVQAAAMRDSIHYYPGN DLHPDLEEPSSLGV in isoform 3.		VSP_033601
	Alternative sequence	135 – 204	70	Missing in isoform 3.		VSP_033602
	Natural variant	154	1	T → S. Corresponds to variant rs12612256 [ dbSNP | Ensembl ].		VAR_043340

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: EF7579A452381E47	FASTA	204	22,423
	10 20 30 40 50 60 MNRVNDPLIF IRDIKPGLKN LNVVFIVLEI GRVTKTKDGH EVRSCKVADK TGSITISVWD 70 80 90 100 110 120 EIGGLIQPGD IIRLTRGYAS MWKGCLTLYT GRGGELQKIG EFCMVYSEVP NFSEPNPDYR 130 140 150 160 170 180 GQQNKGAQSE QKNNSMNSNM GTGTFGPVGN GVHTGPESRE HQFSHAGRSN GRGLINPQLQ 190 200 GTASNQTVMT TISNGRDPRR AFKR
	Isoform 2 [UniParc]. Checksum: 285E087256426CAC	FASTA	124	13,579
	10 20 30 40 50 60 MWKGCLTLYT GRGGELQKIG EFCMVYSEVP NFSEPNPDYR GQQNKGAQSE QKNNSMNSNM 70 80 90 100 110 120 GTGTFGPVGN GVHTGPESRE HQFSHAGRSN GRGLINPQLQ GTASNQTVMT TISNGRDPRR AFKR
	Isoform 3 [UniParc]. Checksum: 9243CCF90A5E06E2	FASTA	134	14,677
	10 20 30 40 50 60 MNRVNDPLIF IRDIKPGLKN LNVVFIVLEI GRVTKTKDGH EVRSCKVADK TGSITISVWD 70 80 90 100 110 120 EIGGLIQPGD IIRLTRGYAS MWKGCLTLYT GRGGELQKIG DLGAVQAAAM RDSIHYYPGN 130 DLHPDLEEPS SLGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Ileal mucosa.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Bone marrow.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Uterus.
[6]	"hSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response." Li Y., Bolderson E., Kumar R., Muniandy P.A., Xue Y., Richard D.J., Seidman M., Pandita T.K., Khanna K.K., Wang W. J. Biol. Chem. 284:23525-23531(2009) [PubMed: 19605351] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SOSS COMPLEX.
[7]	"SOSS complexes participate in the maintenance of genomic stability." Huang J., Gong Z., Ghosal G., Chen J. Mol. Cell 35:384-393(2009) [PubMed: 19683501] [Abstract] Cited for: FUNCTION IN THE SOSS COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SOSS COMPLEX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK026486 mRNA. Translation: BAB15491.1. AL832659 mRNA. Translation: CAH56209.1. AC114778 Genomic DNA. Translation: AAY24348.1. CH471058 Genomic DNA. Translation: EAX10836.1. CH471058 Genomic DNA. Translation: EAX10837.1. CH471058 Genomic DNA. Translation: EAX10838.1. BC017114 mRNA. Translation: AAH17114.1. BC107723 mRNA. Translation: AAI07724.1.
IPI	IPI00010228. IPI00059434. IPI00892743.
RefSeq	NP_001026886.1. NM_001031716.2.
UniGene	Hs.591610.
3D structure databases
ProteinModelPortal	Q96AH0.
SMR	Q96AH0. Positions 9-101.
ModBase	Search...
Protein-protein interaction databases
STRING	Q96AH0.
Polymorphism databases
DMDM	74760705.
Proteomic databases
PRIDE	Q96AH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000425611; ENSP00000403683; ENSG00000173559.
GeneID	64859.
KEGG	hsa:64859.
UCSC	uc002usw.1. human. uc002usx.1. human.
Organism-specific databases
CTD	64859.
GeneCards	GC02P192542.
H-InvDB	HIX0002686.
HGNC	HGNC:26232. OBFC2A.
MIM	612103. gene.
neXtProt	NX_Q96AH0.
PharmGKB	PA143485566.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18195.
GeneTree	ENSGT00390000001913.
HOGENOM	HBG599931.
InParanoid	Q96AH0.
OMA	GTANNQT.
PhylomeDB	Q96AH0.
Gene expression databases
ArrayExpress	Q96AH0.
Bgee	Q96AH0.
CleanEx	HS_OBFC2A.
Genevestigator	Q96AH0.
Family and domain databases
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01336. tRNA_anti. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
NextBio	67011.
SOURCE	Search...

Entry information

Entry name

SOSB2_HUMAN

Accession

Primary (citable) accession number: Q96AH0
Secondary accession number(s): Q658Y8, Q9H5X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.