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Protein

Far upstream element-binding protein 1

Gene

FUBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • single-stranded DNA binding Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • positive regulation of gene expression Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS08703-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Far upstream element-binding protein 1
Short name:
FBP
Short name:
FUSE-binding protein 1
Alternative name(s):
DNA helicase V
Short name:
hDH V
Gene namesi
Name:FUBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4004. FUBP1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8880.
OpenTargetsiENSG00000162613.
PharmGKBiPA28420.

Polymorphism and mutation databases

BioMutaiFUBP1.
DMDMi116241370.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000501352 – 644Far upstream element-binding protein 1Add BLAST643

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei52PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei153PhosphothreonineCombined sources1
Modified residuei321Omega-N-methylarginineBy similarity1
Modified residuei359Omega-N-methylarginineCombined sources1
Modified residuei361Omega-N-methylarginineCombined sources1
Modified residuei363Omega-N-methylarginineCombined sources1
Modified residuei432PhosphothreonineCombined sources1
Modified residuei630PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated. This targets the protein for proteasome-mediated degradation.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ96AE4.
MaxQBiQ96AE4.
PaxDbiQ96AE4.
PeptideAtlasiQ96AE4.
PRIDEiQ96AE4.
TopDownProteomicsiQ96AE4-1. [Q96AE4-1]

2D gel databases

REPRODUCTION-2DPAGEIPI00375441.

PTM databases

iPTMnetiQ96AE4.
PhosphoSitePlusiQ96AE4.
SwissPalmiQ96AE4.

Expressioni

Gene expression databases

BgeeiENSG00000162613.
CleanExiHS_FUBP1.
ExpressionAtlasiQ96AE4. baseline and differential.
GenevisibleiQ96AE4. HS.

Organism-specific databases

HPAiHPA006149.

Interactioni

Subunit structurei

Found in a complex with PUF60 and far upstream element (FUSE) DNA segment. Interacts with PUF60 and JTV1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AIMP2Q131554EBI-711404,EBI-745226
USP22Q9UPT93EBI-711404,EBI-723510

Protein-protein interaction databases

BioGridi114399. 101 interactors.
DIPiDIP-47273N.
IntActiQ96AE4. 44 interactors.
MINTiMINT-1374324.
STRINGi9606.ENSP00000359804.

Structurei

Secondary structure

1644
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 44Combined sources15
Beta strandi101 – 108Combined sources8
Helixi109 – 111Combined sources3
Helixi112 – 116Combined sources5
Helixi118 – 120Combined sources3
Helixi121 – 130Combined sources10
Beta strandi133 – 136Combined sources4
Beta strandi144 – 152Combined sources9
Helixi154 – 168Combined sources15
Beta strandi278 – 283Combined sources6
Helixi284 – 291Combined sources8
Helixi293 – 295Combined sources3
Helixi296 – 305Combined sources10
Beta strandi308 – 312Combined sources5
Beta strandi319 – 327Combined sources9
Helixi329 – 346Combined sources18
Beta strandi378 – 384Combined sources7
Turni385 – 387Combined sources3
Helixi388 – 392Combined sources5
Helixi394 – 396Combined sources3
Helixi397 – 406Combined sources10
Beta strandi409 – 413Combined sources5
Turni417 – 419Combined sources3
Beta strandi424 – 430Combined sources7
Helixi433 – 446Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4WNMR-A278-447[»]
2KXHNMR-B27-52[»]
4LIJX-ray1.80A/B/C86-175[»]
ProteinModelPortaliQ96AE4.
SMRiQ96AE4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96AE4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 164KH 1PROSITE-ProRule annotationAdd BLAST65
Domaini185 – 251KH 2PROSITE-ProRule annotationAdd BLAST67
Domaini275 – 339KH 3PROSITE-ProRule annotationAdd BLAST65
Domaini376 – 443KH 4PROSITE-ProRule annotationAdd BLAST68

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 26Gly-richAdd BLAST14
Compositional biasi349 – 396Gly-richAdd BLAST48
Compositional biasi450 – 560Pro-richAdd BLAST111

Sequence similaritiesi

Contains 4 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
GeneTreeiENSGT00730000110664.
HOGENOMiHOG000231552.
HOVERGENiHBG000625.
InParanoidiQ96AE4.
KOiK13210.
PhylomeDBiQ96AE4.
TreeFamiTF313654.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96AE4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADYSTVPPP SSGSAGGGGG GGGGGGVNDA FKDALQRARQ IAAKIGGDAG
60 70 80 90 100
TSLNSNDYGY GGQKRPLEDG DQPDAKKVAP QNDSFGTQLP PMHQQQSRSV
110 120 130 140 150
MTEEYKVPDG MVGFIIGRGG EQISRIQQES GCKIQIAPDS GGLPERSCML
160 170 180 190 200
TGTPESVQSA KRLLDQIVEK GRPAPGFHHG DGPGNAVQEI MIPASKAGLV
210 220 230 240 250
IGKGGETIKQ LQERAGVKMV MIQDGPQNTG ADKPLRITGD PYKVQQAKEM
260 270 280 290 300
VLELIRDQGG FREVRNEYGS RIGGNEGIDV PIPRFAVGIV IGRNGEMIKK
310 320 330 340 350
IQNDAGVRIQ FKPDDGTTPE RIAQITGPPD RCQHAAEIIT DLLRSVQAGN
360 370 380 390 400
PGGPGPGGRG RGRGQGNWNM GPPGGLQEFN FIVPTGKTGL IIGKGGETIK
410 420 430 440 450
SISQQSGARI ELQRNPPPNA DPNMKLFTIR GTPQQIDYAR QLIEEKIGGP
460 470 480 490 500
VNPLGPPVPH GPHGVPGPHG PPGPPGPGTP MGPYNPAPYN PGPPGPAPHG
510 520 530 540 550
PPAPYAPQGW GNAYPHWQQQ APPDPAKAGT DPNSAAWAAY YAHYYQQQAQ
560 570 580 590 600
PPPAAPAGAP TTTQTNGQGD QQNPAPAGQV DYTKAWEEYY KKMGQAVPAP
610 620 630 640
TGAPPGGQPD YSAAWAEYYR QQAAYYAQTS PQGMPQHPPA PQGQ
Length:644
Mass (Da):67,560
Last modified:October 17, 2006 - v3
Checksum:i1FD422EA2FC49531
GO
Isoform 2 (identifier: Q96AE4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-97: Missing.
     643-644: GQ → CRFDPASIELAL

Note: No experimental confirmation available.
Show »
Length:653
Mass (Da):68,605
Checksum:i52CB50319085539A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049679399I → K.Corresponds to variant rs12748509dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02110797Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_008321643 – 644GQ → CRFDPASIELAL in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05040 mRNA. Translation: AAA17976.2.
AC096948 Genomic DNA. No translation available.
BC017247 mRNA. Translation: AAH17247.1.
CCDSiCCDS683.1. [Q96AE4-1]
PIRiA53184.
RefSeqiNP_001290362.1. NM_001303433.1.
NP_003893.2. NM_003902.4. [Q96AE4-1]
XP_016858232.1. XM_017002743.1. [Q96AE4-2]
UniGeneiHs.567380.
Hs.707742.

Genome annotation databases

EnsembliENST00000294623; ENSP00000294623; ENSG00000162613. [Q96AE4-2]
ENST00000370768; ENSP00000359804; ENSG00000162613. [Q96AE4-1]
GeneIDi8880.
KEGGihsa:8880.
UCSCiuc001dii.4. human. [Q96AE4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05040 mRNA. Translation: AAA17976.2.
AC096948 Genomic DNA. No translation available.
BC017247 mRNA. Translation: AAH17247.1.
CCDSiCCDS683.1. [Q96AE4-1]
PIRiA53184.
RefSeqiNP_001290362.1. NM_001303433.1.
NP_003893.2. NM_003902.4. [Q96AE4-1]
XP_016858232.1. XM_017002743.1. [Q96AE4-2]
UniGeneiHs.567380.
Hs.707742.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4WNMR-A278-447[»]
2KXHNMR-B27-52[»]
4LIJX-ray1.80A/B/C86-175[»]
ProteinModelPortaliQ96AE4.
SMRiQ96AE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114399. 101 interactors.
DIPiDIP-47273N.
IntActiQ96AE4. 44 interactors.
MINTiMINT-1374324.
STRINGi9606.ENSP00000359804.

PTM databases

iPTMnetiQ96AE4.
PhosphoSitePlusiQ96AE4.
SwissPalmiQ96AE4.

Polymorphism and mutation databases

BioMutaiFUBP1.
DMDMi116241370.

2D gel databases

REPRODUCTION-2DPAGEIPI00375441.

Proteomic databases

EPDiQ96AE4.
MaxQBiQ96AE4.
PaxDbiQ96AE4.
PeptideAtlasiQ96AE4.
PRIDEiQ96AE4.
TopDownProteomicsiQ96AE4-1. [Q96AE4-1]

Protocols and materials databases

DNASUi8880.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000294623; ENSP00000294623; ENSG00000162613. [Q96AE4-2]
ENST00000370768; ENSP00000359804; ENSG00000162613. [Q96AE4-1]
GeneIDi8880.
KEGGihsa:8880.
UCSCiuc001dii.4. human. [Q96AE4-1]

Organism-specific databases

CTDi8880.
DisGeNETi8880.
GeneCardsiFUBP1.
HGNCiHGNC:4004. FUBP1.
HPAiHPA006149.
MIMi603444. gene.
neXtProtiNX_Q96AE4.
OpenTargetsiENSG00000162613.
PharmGKBiPA28420.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
GeneTreeiENSGT00730000110664.
HOGENOMiHOG000231552.
HOVERGENiHBG000625.
InParanoidiQ96AE4.
KOiK13210.
PhylomeDBiQ96AE4.
TreeFamiTF313654.

Enzyme and pathway databases

BioCyciZFISH:HS08703-MONOMER.

Miscellaneous databases

ChiTaRSiFUBP1. human.
EvolutionaryTraceiQ96AE4.
GeneWikiiFar_upstream_element-binding_protein_1.
GenomeRNAii8880.
PROiQ96AE4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162613.
CleanExiHS_FUBP1.
ExpressionAtlasiQ96AE4. baseline and differential.
GenevisibleiQ96AE4. HS.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUBP1_HUMAN
AccessioniPrimary (citable) accession number: Q96AE4
Secondary accession number(s): Q12828
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 161 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.