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Protein

Protein N-terminal asparagine amidohydrolase

Gene

NTAN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.1 Publication

Enzyme regulationi

Inhibited by micromolar concentrations of copper and zinc ions.1 Publication

pH dependencei

Optimum pH is 6.0-7.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei75 – 751Essential for catalytic activity

GO - Molecular functioni

  1. protein-N-terminal asparagine amidohydrolase activity Source: Ensembl

GO - Biological processi

  1. adult locomotory behavior Source: Ensembl
  2. memory Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal asparagine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal asparagine amidohydrolase
Short name:
PNAA
Protein NH2-terminal asparagine deamidase
Short name:
PNAD
Short name:
Protein N-terminal Asn amidase
Short name:
Protein N-terminal asparagine amidase
Short name:
Protein NTN-amidase
Gene namesi
Name:NTAN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 16, Unplaced

Organism-specific databases

HGNCiHGNC:29909. NTAN1.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21P → G: 3-fold reduction in catalytic activity. 1 Publication
Mutagenesisi75 – 751C → A, S or T: Abolishes catalytic activity. 1 Publication

Organism-specific databases

PharmGKBiPA134863573.

Polymorphism and mutation databases

DMDMi37082118.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 310309Protein N-terminal asparagine amidohydrolasePRO_0000057971Add
BLAST

Proteomic databases

MaxQBiQ96AB6.
PaxDbiQ96AB6.
PRIDEiQ96AB6.

PTM databases

PhosphoSiteiQ96AB6.

Expressioni

Gene expression databases

BgeeiQ96AB6.
CleanExiHS_NTAN1.
ExpressionAtlasiQ96AB6. baseline and differential.
GenevestigatoriQ96AB6.

Organism-specific databases

HPAiCAB029997.
HPA051418.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi125835. 1 interaction.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG76939.
GeneTreeiENSGT00390000016730.
HOGENOMiHOG000047800.
HOVERGENiHBG052674.
InParanoidiQ96AB6.
KOiK14662.
OMAiAGGPMIS.
OrthoDBiEOG773XGD.
PhylomeDBiQ96AB6.
TreeFamiTF325597.

Family and domain databases

InterProiIPR026750. NTAN1.
[Graphical view]
PANTHERiPTHR12498. PTHR12498. 1 hit.
PfamiPF14736. N_Asn_amidohyd. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96AB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ
60 70 80 90 100
QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA
110 120 130 140 150
EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ
160 170 180 190 200
EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE
210 220 230 240 250
EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL
260 270 280 290 300
ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG
310
LWEKISSPGS
Length:310
Mass (Da):34,677
Last modified:January 23, 2007 - v3
Checksum:iC7FE8C550883DFC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61E → D in AAP97215 (Ref. 1) Curated
Sequence conflicti14 – 141Q → R in AAP97215 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti283 – 2831H → N.
Corresponds to variant rs1136001 [ dbSNP | Ensembl ].
VAR_051244
Natural varianti287 – 2871S → P.
Corresponds to variant rs1135999 [ dbSNP | Ensembl ].
VAR_051245

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092440 mRNA. Translation: AAP97215.1.
BC017336 mRNA. Translation: AAH17336.1.
CCDSiCCDS10558.1.
RefSeqiNP_001257695.1. NM_001270766.1.
NP_001257696.1. NM_001270767.1.
NP_775745.1. NM_173474.3.
UniGeneiHs.592045.

Genome annotation databases

EnsembliENST00000287706; ENSP00000287706; ENSG00000157045.
ENST00000620176; ENSP00000484380; ENSG00000275779.
GeneIDi123803.
KEGGihsa:123803.
UCSCiuc002ddd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092440 mRNA. Translation: AAP97215.1.
BC017336 mRNA. Translation: AAH17336.1.
CCDSiCCDS10558.1.
RefSeqiNP_001257695.1. NM_001270766.1.
NP_001257696.1. NM_001270767.1.
NP_775745.1. NM_173474.3.
UniGeneiHs.592045.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125835. 1 interaction.

PTM databases

PhosphoSiteiQ96AB6.

Polymorphism and mutation databases

DMDMi37082118.

Proteomic databases

MaxQBiQ96AB6.
PaxDbiQ96AB6.
PRIDEiQ96AB6.

Protocols and materials databases

DNASUi123803.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287706; ENSP00000287706; ENSG00000157045.
ENST00000620176; ENSP00000484380; ENSG00000275779.
GeneIDi123803.
KEGGihsa:123803.
UCSCiuc002ddd.4. human.

Organism-specific databases

CTDi123803.
GeneCardsiGC16M015131.
HGNCiHGNC:29909. NTAN1.
HPAiCAB029997.
HPA051418.
MIMi615367. gene.
neXtProtiNX_Q96AB6.
PharmGKBiPA134863573.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG76939.
GeneTreeiENSGT00390000016730.
HOGENOMiHOG000047800.
HOVERGENiHBG052674.
InParanoidiQ96AB6.
KOiK14662.
OMAiAGGPMIS.
OrthoDBiEOG773XGD.
PhylomeDBiQ96AB6.
TreeFamiTF325597.

Miscellaneous databases

GenomeRNAii123803.
NextBioi81157.
PROiQ96AB6.
SOURCEiSearch...

Gene expression databases

BgeeiQ96AB6.
CleanExiHS_NTAN1.
ExpressionAtlasiQ96AB6. baseline and differential.
GenevestigatoriQ96AB6.

Family and domain databases

InterProiIPR026750. NTAN1.
[Graphical view]
PANTHERiPTHR12498. PTHR12498. 1 hit.
PfamiPF14736. N_Asn_amidohyd. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a novel human cDNA homology to murine N-terminal asparagine amidohydrolase (Ntan1) mRNA."
    Jiang C.L., Yu L., Fan Y.X., Tu Q., Jiang J.X., Zhao S.Y.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  3. "Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase."
    Cantor J.R., Stone E.M., Georgiou G.
    Biochemistry 50:3025-3033(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, REMOVAL OF INITIATOR METHIONINE, MUTAGENESIS OF PRO-2 AND CYS-75.

Entry informationi

Entry nameiNTAN1_HUMAN
AccessioniPrimary (citable) accession number: Q96AB6
Secondary accession number(s): Q7Z4Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.