Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q96AB6

- NTAN1_HUMAN

UniProt

Q96AB6 - NTAN1_HUMAN

Protein

Protein N-terminal asparagine amidohydrolase

Gene

NTAN1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.1 Publication

    Enzyme regulationi

    Inhibited by micromolar concentrations of copper and zinc ions.1 Publication

    pH dependencei

    Optimum pH is 6.0-7.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei75 – 751Essential for catalytic activity

    GO - Molecular functioni

    1. protein-N-terminal asparagine amidohydrolase activity Source: Ensembl

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. memory Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein N-terminal asparagine amidohydrolase (EC:3.5.1.-)
    Alternative name(s):
    Protein NH2-terminal asparagine amidohydrolase
    Short name:
    PNAA
    Protein NH2-terminal asparagine deamidase
    Short name:
    PNAD
    Short name:
    Protein N-terminal Asn amidase
    Short name:
    Protein N-terminal asparagine amidase
    Short name:
    Protein NTN-amidase
    Gene namesi
    Name:NTAN1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:29909. NTAN1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21P → G: 3-fold reduction in catalytic activity. 1 Publication
    Mutagenesisi75 – 751C → A, S or T: Abolishes catalytic activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA134863573.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 310309Protein N-terminal asparagine amidohydrolasePRO_0000057971Add
    BLAST

    Proteomic databases

    MaxQBiQ96AB6.
    PaxDbiQ96AB6.
    PRIDEiQ96AB6.

    PTM databases

    PhosphoSiteiQ96AB6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ96AB6.
    BgeeiQ96AB6.
    CleanExiHS_NTAN1.
    GenevestigatoriQ96AB6.

    Organism-specific databases

    HPAiCAB029997.
    HPA051418.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ96AB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG76939.
    HOGENOMiHOG000047800.
    HOVERGENiHBG052674.
    InParanoidiQ96AB6.
    KOiK14662.
    OMAiTHCDGSD.
    OrthoDBiEOG773XGD.
    PhylomeDBiQ96AB6.
    TreeFamiTF325597.

    Family and domain databases

    InterProiIPR026750. NTAN1.
    [Graphical view]
    PANTHERiPTHR12498. PTHR12498. 1 hit.
    PfamiPF14736. N_Asn_amidohyd. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q96AB6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ    50
    QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA 100
    EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ 150
    EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE 200
    EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL 250
    ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG 300
    LWEKISSPGS 310
    Length:310
    Mass (Da):34,677
    Last modified:January 23, 2007 - v3
    Checksum:iC7FE8C550883DFC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61E → D in AAP97215. 1 PublicationCurated
    Sequence conflicti14 – 141Q → R in AAP97215. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti283 – 2831H → N.
    Corresponds to variant rs1136001 [ dbSNP | Ensembl ].
    VAR_051244
    Natural varianti287 – 2871S → P.
    Corresponds to variant rs1135999 [ dbSNP | Ensembl ].
    VAR_051245

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF092440 mRNA. Translation: AAP97215.1.
    BC017336 mRNA. Translation: AAH17336.1.
    CCDSiCCDS10558.1.
    RefSeqiNP_001257695.1. NM_001270766.1.
    NP_001257696.1. NM_001270767.1.
    NP_775745.1. NM_173474.3.
    UniGeneiHs.592045.

    Genome annotation databases

    EnsembliENST00000287706; ENSP00000287706; ENSG00000157045.
    GeneIDi123803.
    KEGGihsa:123803.
    UCSCiuc002ddd.4. human.

    Polymorphism databases

    DMDMi37082118.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF092440 mRNA. Translation: AAP97215.1 .
    BC017336 mRNA. Translation: AAH17336.1 .
    CCDSi CCDS10558.1.
    RefSeqi NP_001257695.1. NM_001270766.1.
    NP_001257696.1. NM_001270767.1.
    NP_775745.1. NM_173474.3.
    UniGenei Hs.592045.

    3D structure databases

    ProteinModelPortali Q96AB6.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q96AB6.

    Polymorphism databases

    DMDMi 37082118.

    Proteomic databases

    MaxQBi Q96AB6.
    PaxDbi Q96AB6.
    PRIDEi Q96AB6.

    Protocols and materials databases

    DNASUi 123803.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000287706 ; ENSP00000287706 ; ENSG00000157045 .
    GeneIDi 123803.
    KEGGi hsa:123803.
    UCSCi uc002ddd.4. human.

    Organism-specific databases

    CTDi 123803.
    GeneCardsi GC16M015131.
    HGNCi HGNC:29909. NTAN1.
    HPAi CAB029997.
    HPA051418.
    MIMi 615367. gene.
    neXtProti NX_Q96AB6.
    PharmGKBi PA134863573.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG76939.
    HOGENOMi HOG000047800.
    HOVERGENi HBG052674.
    InParanoidi Q96AB6.
    KOi K14662.
    OMAi THCDGSD.
    OrthoDBi EOG773XGD.
    PhylomeDBi Q96AB6.
    TreeFami TF325597.

    Miscellaneous databases

    GenomeRNAii 123803.
    NextBioi 81157.
    PROi Q96AB6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q96AB6.
    Bgeei Q96AB6.
    CleanExi HS_NTAN1.
    Genevestigatori Q96AB6.

    Family and domain databases

    InterProi IPR026750. NTAN1.
    [Graphical view ]
    PANTHERi PTHR12498. PTHR12498. 1 hit.
    Pfami PF14736. N_Asn_amidohyd. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a novel human cDNA homology to murine N-terminal asparagine amidohydrolase (Ntan1) mRNA."
      Jiang C.L., Yu L., Fan Y.X., Tu Q., Jiang J.X., Zhao S.Y.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    3. "Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase."
      Cantor J.R., Stone E.M., Georgiou G.
      Biochemistry 50:3025-3033(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, REMOVAL OF INITIATOR METHIONINE, MUTAGENESIS OF PRO-2 AND CYS-75.

    Entry informationi

    Entry nameiNTAN1_HUMAN
    AccessioniPrimary (citable) accession number: Q96AB6
    Secondary accession number(s): Q7Z4Z0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 84 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3