Q96A65 (EXOC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exocyst complex component 4

Alternative name(s):
Exocyst complex component Sec8

Gene names

Name:	EXOC4
Synonyms:	KIAA1699, SEC8, SEC8L1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	974 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane By similarity.
Subunit structure	The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8 By similarity. Interacts with BIRC6/bruce. Interacts with MYRIP By similarity. Ref.10
Sequence similarities	Belongs to the SEC8 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay. Source: LIFEdb exocyst Inferred from electronic annotation. Source: InterPro growth cone membrane Inferred from electronic annotation. Source: Compara myelin sheath abaxonal region Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BIRC6	Q9NR09	3	EBI-355383,EBI-1765160

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 974	973	Exocyst complex component 4		PRO_0000118934
Regions
	Coiled coil	32 – 114	83	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.6 Ref.13
	Modified residue	9	1	N6-acetyllysine Ref.13
	Modified residue	237	1	Phosphothreonine Ref.9 Ref.11 Ref.12 Ref.14
Natural variations
	Natural variant	220	1	S → F in a colorectal cancer sample; somatic mutation. Ref.16		VAR_036292
	Natural variant	599	1	A → T in a colorectal cancer sample; somatic mutation. Ref.16		VAR_036293
Experimental info
	Sequence conflict	293	1	P → S in CAD39134. Ref.3
	Sequence conflict	338	1	L → T in AAF66445. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q96A65 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 122CD929C2635786	FASTA	974	110,498
	10 20 30 40 50 60 MAAEAAGGKY RSTVSKSKDP SGLLISVIRT LSTSDDVEDR ENEKGRLEEA YEKCDRDLDE 70 80 90 100 110 120 LIVQHYTELT TAIRTYQSIT ERITNSRNKI KQVKENLLSC KMLLHCKRDE LRKLWIEGIE 130 140 150 160 170 180 HKHVLNLLDE IENIKQVPQK LEQCMASKHY LSATDMLVSA VESLEGPLLQ VEGLSDLRLE 190 200 210 220 230 240 LHSKKMNLHL VLIDELHRHL YIKSTSRVVQ RNKEKGKISS LVKDASVPLI DVTNLPTPRK 250 260 270 280 290 300 FLDTSHYSTA GSSSVREINL QDIKEDLELD PEENSTLFMG ILIKGLAKLK KIPETVKAII 310 320 330 340 350 360 ERLEQELKQI VKRSTTQVAD SGYQRGENVT VENQPRLLLE LLELLFDKFN AVAAAHSVVL 370 380 390 400 410 420 GYLQDTVVTP LTQQEDIKLY DMADVWVKIQ DVLQMLLTEY LDMKNTRTAS EPSAQLSYAS 430 440 450 460 470 480 TGREFAAFFA KKKPQRPKNS LFKFESSSHA ISMSAYLREQ RRELYSRSGE LQGGPDDNLI 490 500 510 520 530 540 EGGGTKFVCK PGARNITVIF HPLLRFIQEI EHALGLGPAK QCPLREFLTV YIKNIFLNQV 550 560 570 580 590 600 LAEINKEIEG VTKTSDPLKI LANADTMKVL GVQRPLLQST IIVEKTVQDL LNLMHDLSAY 610 620 630 640 650 660 SDQFLNMVCV KLQEYKDTCT AAYRGIVQSE EKLVISASWA KDDDISRLLK SLPNWMNMAQ 670 680 690 700 710 720 PKQLRPKREE EEDFIRAAFG KESEVLIGNL GDKLIPPQDI LRDVSDLKAL ANMHESLEWL 730 740 750 760 770 780 ASRTKSAFSN LSTSQMLSPA QDSHTNTDLP PVSEQIMQTL SELAKSFQDM ADRCLLVLHL 790 800 810 820 830 840 EVRVHCFHYL IPLAKEGNYA IVANVESMDY DPLVVKLNKD ISAIEEAMSA SLQQHKFQYI 850 860 870 880 890 900 FEGLGHLISC ILINGAQYFR RISESGIKKM CRNIFVLQQN LTNITMSREA DLDFARQYYE 910 920 930 940 950 960 MLYNTADELL NLVVDQGVKY TELEYIHALT LLHRSQTGVG ELTTQNTRLQ RLKEIICEQA 970 AIKQATKDKK ITTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human secretory protein Sec8 related to testis development." Sha J.H. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma and Skeletal muscle.
[4]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Bienvenut W.V., Campbell A., Ozanne B.W. Submitted (JUN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 224-239; 314-325 AND 889-896, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Foreskin fibroblast.
[7]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-974. Tissue: Brain.
[8]	Luo W.Q., Chen J.H., Huang X.W., Zhou Y., Zhou H.J., Hu S.N., Yuan J.G. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 338-974.
[9]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Final stages of cytokinesis and midbody ring formation are controlled by BRUCE." Pohl C., Jentsch S. Cell 132:832-845(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BIRC6/BRUCE.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 AND LYS-9, MASS SPECTROMETRY.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-220 AND THR-599.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF380839 mRNA. Translation: AAK57456.1. AK022751 mRNA. Translation: BAB14225.1. AK027688 mRNA. Translation: BAB55298.1. AL831989 mRNA. Translation: CAD89977.1. AL834475 mRNA. Translation: CAD39134.1. CH236950 Genomic DNA. Translation: EAL24073.1. CH471070 Genomic DNA. Translation: EAW83804.1. AB051486 mRNA. Translation: BAB21790.1. AF132734 mRNA. Translation: AAF66445.1.
IPI	IPI00059279.
RefSeq	NP_068579.3. NM_021807.3.
UniGene	Hs.321273.
3D structure databases
ProteinModelPortal	Q96A65.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32976N.
IntAct	Q96A65. 15 interactions.
STRING	9606.ENSP00000253861.
PTM databases
PhosphoSite	Q96A65.
Polymorphism databases
DMDM	24418674.
Proteomic databases
PaxDb	Q96A65.
PRIDE	Q96A65.
Protocols and materials databases
DNASU	60412.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253861; ENSP00000253861; ENSG00000131558.
GeneID	60412.
KEGG	hsa:60412.
UCSC	uc003vrk.3. human.
Organism-specific databases
CTD	60412.
GeneCards	GC07P132937.
HGNC	HGNC:30389. EXOC4.
HPA	HPA031443.
MIM	608185. gene.
neXtProt	NX_Q96A65.
PharmGKB	PA134944654.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG270077.
HOGENOM	HOG000044203.
HOVERGEN	HBG036151.
InParanoid	Q96A65.
KO	K06111.
OMA	KQSLFKF.
OrthoDB	EOG4GB75C.
PhylomeDB	Q96A65.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6_traffickingpathway. Arf6 trafficking events. insulin_pathway. Insulin Pathway.
Reactome	REACT_11123. Membrane Trafficking. REACT_116125. Disease.
Gene expression databases
ArrayExpress	Q96A65.
Bgee	Q96A65.
CleanEx	HS_EXOC4.
Genevestigator	Q96A65.
GermOnline	ENSG00000131558. Homo sapiens.
Family and domain databases
InterPro	IPR007191. Sec8_exocyst. [Graphical view]
Pfam	PF04048. Sec8_exocyst. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EXOC4. human.
GenomeRNAi	60412.
NextBio	65320.
SOURCE	Search...

Entry information

Entry name

EXOC4_HUMAN

Accession

Primary (citable) accession number: Q96A65
Secondary accession number(s): Q541U8 , Q9C0G4, Q9H9K0, Q9P102

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.