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Q96A56 (T53I1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tumor protein p53-inducible nuclear protein 1
Alternative name(s):
Stress-induced protein
p53-dependent damage-inducible nuclear protein 1
Short name=p53DINP1
Gene names
Name:TP53INP1
Synonyms:P53DINP1, SIP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. Ref.1 Ref.8 Ref.9

Subunit structure

Interacts with p53/TP53 and HIPK2. Interacts with PRKCG, GABARAP, GABARAPL1, GABARAPL2, MAP1LC3A, MAP1LC3B AND MAP1LC3C. Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasmcytosol. Nucleus. NucleusPML body. Cytoplasmic vesicleautophagosome. Note: Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation. Ref.1 Ref.6 Ref.9

Tissue specificity

Ubiquitously expressed. Ref.1 Ref.2

Induction

By adriamycin, gamma irradiation and H2O2, in a p53/TP53-dependent way. At lower levels by UV irradiation. By TP73. Ref.1 Ref.2

Domain

The LC3 interacting region (LIR) motif mediates interaction with GABARAP, GABARAPL1, GABARAPL2, MAP1LC3A, MAP1LC3B and MAP1LC3C.

Ontologies

Keywords
   Biological processApoptosis
Autophagy
Transcription
Transcription regulation
   Cellular componentCytoplasm
Cytoplasmic vesicle
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseTumor suppressor
   Molecular functionActivator
Antioxidant
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

autophagic cell death

Inferred from mutant phenotype Ref.8. Source: UniProtKB

autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

cell cycle arrest

Traceable author statement Ref.8. Source: UniProtKB

cellular response to UV

Inferred from electronic annotation. Source: Ensembl

cellular response to ethanol

Inferred from electronic annotation. Source: Ensembl

cellular response to hydroperoxide

Inferred from electronic annotation. Source: Ensembl

cellular response to methyl methanesulfonate

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of autophagy

Inferred from mutant phenotype Ref.8Ref.9. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.9. Source: UniProtKB

response to heat

Inferred from electronic annotation. Source: Ensembl

response to stress

Traceable author statement Ref.8. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentPML body

Inferred from electronic annotation. Source: UniProtKB-SubCell

autophagic vacuole

Inferred from direct assay Ref.8Ref.9. Source: UniProtKB

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Inferred from direct assay Ref.9. Source: UniProtKB

nucleus

Inferred from direct assay Ref.9. Source: UniProtKB

   Molecular_functionantioxidant activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.8Ref.9. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q96A56-1)

Also known as: p53DINP1a; alpha; SIP27; TEAP;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q96A56-2)

Also known as: p53DINP1b; beta; SIP18;

The sequence of this isoform differs from the canonical sequence as follows:
     159-240: VEAQNEMGQH...HQPCPRQYNY → ARKSCL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Tumor protein p53-inducible nuclear protein 1
PRO_0000072406

Regions

Motif25 – 3713LIR
Compositional bias14 – 174Poly-Ser
Compositional bias20 – 6142Glu-rich

Natural variations

Alternative sequence159 – 24082VEAQN…RQYNY → ARKSCL in isoform 2.
VSP_013176
Natural variant751C → R.
Corresponds to variant rs11991800 [ dbSNP | Ensembl ].
VAR_051404

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (p53DINP1a) (alpha) (SIP27) (TEAP) [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 1950D66EDDB4A186

FASTA24027,366
        10         20         30         40         50         60 
MFQRLNKMFV GEVSSSSNQE PEFNEKEDDE WILVDFIDTC TGFSAEEEEE EEDISEESPT 

        70         80         90        100        110        120 
EHPSVFSCLP ASLECLADTS DSCFLQFESC PMEESWFITP PPCFTAGGLT TIKVETSPME 

       130        140        150        160        170        180 
NLLIEHPSMS VYAVHNSCPG LSEATRGTDE LHSPSSPRVE AQNEMGQHIH CYVAALAAHT 

       190        200        210        220        230        240 
TFLEQPKSFR PSQWIKEHSE RQPLNRNSLR RQNLTRDCHP RQVKHNGWVV HQPCPRQYNY 

« Hide

Isoform 2 (p53DINP1b) (beta) (SIP18) [UniParc].

Checksum: 8C5B27F2CFB47B8E
Show »

FASTA16418,244

References

« Hide 'large scale' references
[1]"p53DINP1, a p53-inducible gene, regulate p53-dependent apoptosis."
Okamura S., Arakawa H., Tanaka T., Nakanishi H., Ng C.C., Taya Y., Monden M., Nakamura Y.
Mol. Cell 8:85-94(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, FUNCTION.
Tissue: Thymus.
[2]"p53-dependent expression of the stress-induced protein (SIP)."
Tomasini R., Azizi Samir A.L., Pebusque M.-J., Calvo E.L., Totaro S., Dagorn J.-C., Dusetti N.J., Iovanna J.L.
Eur. J. Cell Biol. 81:294-301(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION, TISSUE SPECIFICITY.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Trachea.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[6]"TP53INP1s and homeodomain-interacting protein kinase-2 (HIPK2) are partners in regulating p53 activity."
Tomasini R., Samir A.A., Carrier A., Isnardon D., Cecchinelli B., Soddu S., Malissen B., Dagorn J.-C., Iovanna J.L., Dusetti N.J.
J. Biol. Chem. 278:37722-37729(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53 AND HIPK2, SUBCELLULAR LOCATION.
[7]"Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage."
Yoshida K., Liu H., Miki Y.
J. Biol. Chem. 281:5734-5740(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRKCG.
[8]"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."
Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J.
Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GABARAP; GABARAPL2 AND MAP1LC3A.
[9]"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription."
Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A.
PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GABARAP; GABARAPL1; GABARAPL2; MAP1LC3A; MAP1LC3B AND MAP1LC3C.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017926 mRNA. Translation: BAB62149.1.
AB017927 mRNA. Translation: BAB62150.1.
AB062056 Genomic DNA. Translation: BAB62151.1.
AB062056 Genomic DNA. Translation: BAB62152.1.
AF409114 mRNA. Translation: AAL04513.1.
AF409115 mRNA. Translation: AAL04514.1.
AK315071 mRNA. Translation: BAG37542.1.
CH471060 Genomic DNA. Translation: EAW91740.1.
BC074813 mRNA. Translation: AAH74813.1.
BC074868 mRNA. Translation: AAH74868.1.
CCDSCCDS47899.1. [Q96A56-2]
CCDS6265.1. [Q96A56-1]
RefSeqNP_001129205.1. NM_001135733.1. [Q96A56-2]
NP_150601.1. NM_033285.3. [Q96A56-1]
UniGeneHs.492261.

3D structure databases

ProteinModelPortalQ96A56.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125152. 3 interactions.
STRING9606.ENSP00000344215.

Polymorphism databases

DMDM61216823.

Proteomic databases

PRIDEQ96A56.

Protocols and materials databases

DNASU94241.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000342697; ENSP00000344215; ENSG00000164938. [Q96A56-1]
ENST00000448464; ENSP00000390063; ENSG00000164938. [Q96A56-2]
GeneID94241.
KEGGhsa:94241.
UCSCuc003yhg.3. human. [Q96A56-1]
uc003yhh.3. human. [Q96A56-2]

Organism-specific databases

CTD94241.
GeneCardsGC08M095938.
HGNCHGNC:18022. TP53INP1.
HPAHPA005856.
MIM606185. gene.
neXtProtNX_Q96A56.
PharmGKBPA38278.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39796.
HOGENOMHOG000010288.
HOVERGENHBG055647.
InParanoidQ96A56.
KOK15310.
OMAWAVHQPC.
OrthoDBEOG7MSMPN.
PhylomeDBQ96A56.
TreeFamTF333017.

Gene expression databases

ArrayExpressQ96A56.
BgeeQ96A56.
CleanExHS_TP53INP1.
GenevestigatorQ96A56.

Family and domain databases

InterProIPR029431. TP53INP.
[Graphical view]
PfamPF14839. DOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTP53INP1. human.
GeneWikiTP53INP1.
GenomeRNAi94241.
NextBio78499.
PROQ96A56.
SOURCESearch...

Entry information

Entry nameT53I1_HUMAN
AccessionPrimary (citable) accession number: Q96A56
Secondary accession number(s): B2RCE5, Q969R9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM