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Protein

Mitoferrin-2

Gene

SLC25A28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

ReactomeiR-HSA-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Protein family/group databases

TCDBi2.A.29.5.8. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitoferrin-2
Alternative name(s):
Mitochondrial RNA-splicing protein 3/4 homolog
Short name:
MRS3/4
Short name:
hMRS3/4
Mitochondrial iron transporter 2
Solute carrier family 25 member 28
Gene namesi
Name:SLC25A28
Synonyms:MFRN2
ORF Names:NPD016
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23472. SLC25A28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei72 – 9120Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei133 – 15220Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei170 – 18920Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei227 – 24620Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei261 – 28020Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei327 – 34620Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134867966.

Polymorphism and mutation databases

BioMutaiSLC25A28.
DMDMi74751734.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Mitoferrin-2PRO_0000235255Add
BLAST

Proteomic databases

EPDiQ96A46.
MaxQBiQ96A46.
PaxDbiQ96A46.
PRIDEiQ96A46.

PTM databases

iPTMnetiQ96A46.
PhosphoSiteiQ96A46.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart.1 Publication

Gene expression databases

BgeeiQ96A46.
CleanExiHS_SLC25A28.
ExpressionAtlasiQ96A46. baseline and differential.
GenevisibleiQ96A46. HS.

Interactioni

Protein-protein interaction databases

BioGridi123626. 3 interactions.
STRINGi9606.ENSP00000359526.

Structurei

3D structure databases

ProteinModelPortaliQ96A46.
SMRiQ96A46. Positions 70-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 15889Solcar 1Add
BLAST
Repeati168 – 25285Solcar 2Add
BLAST
Repeati259 – 35294Solcar 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 4743Gly-richAdd
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0760. Eukaryota.
ENOG410XNT2. LUCA.
GeneTreeiENSGT00550000074721.
HOGENOMiHOG000038810.
HOVERGENiHBG079464.
InParanoidiQ96A46.
KOiK15113.
OMAiPMRGLNI.
OrthoDBiEOG77DJ7B.
PhylomeDBiQ96A46.
TreeFamiTF314118.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96A46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELEGRGAGG VAGGPAAGPG RSPGESALLD GWLQRGVGRG AGGGEAGACR
60 70 80 90 100
PPVRQDPDSG PDYEALPAGA TVTTHMVAGA VAGILEHCVM YPIDCVKTRM
110 120 130 140 150
QSLQPDPAAR YRNVLEALWR IIRTEGLWRP MRGLNVTATG AGPAHALYFA
160 170 180 190 200
CYEKLKKTLS DVIHPGGNSH IANGAAGCVA TLLHDAAMNP AEVVKQRMQM
210 220 230 240 250
YNSPYHRVTD CVRAVWQNEG AGAFYRSYTT QLTMNVPFQA IHFMTYEFLQ
260 270 280 290 300
EHFNPQRRYN PSSHVLSGAC AGAVAAAATT PLDVCKTLLN TQESLALNSH
310 320 330 340 350
ITGHITGMAS AFRTVYQVGG VTAYFRGVQA RVIYQIPSTA IAWSVYEFFK
360
YLITKRQEEW RAGK
Length:364
Mass (Da):39,272
Last modified:December 1, 2001 - v1
Checksum:i22049C4F8DD7A577
GO
Isoform 2 (identifier: Q96A46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-187: Missing.

Show »
Length:177
Mass (Da):20,045
Checksum:iE5370283AAF732E3
GO
Isoform 3 (identifier: Q96A46-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-188: Missing.
     189-192: NPAE → MALL

Show »
Length:176
Mass (Da):19,931
Checksum:i4A4831AC572309D4
GO

Sequence cautioni

The sequence AAH47312.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI16900.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 188188Missing in isoform 3. 2 PublicationsVSP_018413Add
BLAST
Alternative sequencei1 – 187187Missing in isoform 2. 1 PublicationVSP_018414Add
BLAST
Alternative sequencei189 – 1924NPAE → MALL in isoform 3. 2 PublicationsVSP_018415

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327402 mRNA. Translation: AAK49519.1.
AF327403 mRNA. Translation: AAK49520.1.
AJ303077 mRNA. Translation: CAC27996.1.
AJ303078 mRNA. Translation: CAC27997.1.
AF267854 mRNA. Translation: AAG44723.1.
AL831943 mRNA. Translation: CAH10774.1.
AL353719 Genomic DNA. Translation: CAI16900.1. Sequence problems.
AL353719 Genomic DNA. Translation: CAI16901.1.
BC047312 mRNA. Translation: AAH47312.1. Different initiation.
BC058937 mRNA. Translation: AAH58937.1.
BC076399 mRNA. Translation: AAH76399.1.
BC094821 mRNA. Translation: AAH94821.1.
CCDSiCCDS41559.1. [Q96A46-1]
RefSeqiNP_112489.3. NM_031212.3. [Q96A46-1]
UniGeneiHs.403790.
Hs.736801.

Genome annotation databases

EnsembliENST00000370495; ENSP00000359526; ENSG00000155287. [Q96A46-1]
GeneIDi81894.
KEGGihsa:81894.
UCSCiuc001kpx.3. human. [Q96A46-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327402 mRNA. Translation: AAK49519.1.
AF327403 mRNA. Translation: AAK49520.1.
AJ303077 mRNA. Translation: CAC27996.1.
AJ303078 mRNA. Translation: CAC27997.1.
AF267854 mRNA. Translation: AAG44723.1.
AL831943 mRNA. Translation: CAH10774.1.
AL353719 Genomic DNA. Translation: CAI16900.1. Sequence problems.
AL353719 Genomic DNA. Translation: CAI16901.1.
BC047312 mRNA. Translation: AAH47312.1. Different initiation.
BC058937 mRNA. Translation: AAH58937.1.
BC076399 mRNA. Translation: AAH76399.1.
BC094821 mRNA. Translation: AAH94821.1.
CCDSiCCDS41559.1. [Q96A46-1]
RefSeqiNP_112489.3. NM_031212.3. [Q96A46-1]
UniGeneiHs.403790.
Hs.736801.

3D structure databases

ProteinModelPortaliQ96A46.
SMRiQ96A46. Positions 70-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123626. 3 interactions.
STRINGi9606.ENSP00000359526.

Protein family/group databases

TCDBi2.A.29.5.8. the mitochondrial carrier (mc) family.

PTM databases

iPTMnetiQ96A46.
PhosphoSiteiQ96A46.

Polymorphism and mutation databases

BioMutaiSLC25A28.
DMDMi74751734.

Proteomic databases

EPDiQ96A46.
MaxQBiQ96A46.
PaxDbiQ96A46.
PRIDEiQ96A46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370495; ENSP00000359526; ENSG00000155287. [Q96A46-1]
GeneIDi81894.
KEGGihsa:81894.
UCSCiuc001kpx.3. human. [Q96A46-1]

Organism-specific databases

CTDi81894.
GeneCardsiSLC25A28.
H-InvDBHIX0009111.
HGNCiHGNC:23472. SLC25A28.
MIMi609767. gene.
neXtProtiNX_Q96A46.
PharmGKBiPA134867966.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0760. Eukaryota.
ENOG410XNT2. LUCA.
GeneTreeiENSGT00550000074721.
HOGENOMiHOG000038810.
HOVERGENiHBG079464.
InParanoidiQ96A46.
KOiK15113.
OMAiPMRGLNI.
OrthoDBiEOG77DJ7B.
PhylomeDBiQ96A46.
TreeFamiTF314118.

Enzyme and pathway databases

ReactomeiR-HSA-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Miscellaneous databases

ChiTaRSiSLC25A28. human.
GenomeRNAii81894.
NextBioi72253.
PROiQ96A46.
SOURCEiSearch...

Gene expression databases

BgeeiQ96A46.
CleanExiHS_SLC25A28.
ExpressionAtlasiQ96A46. baseline and differential.
GenevisibleiQ96A46. HS.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel human putative mitochondrial transporter homologous to the yeast mitochondrial RNA splicing proteins 3 and 4."
    Li F.-Y., Nikali K., Gregan J., Leibiger I., Leibiger B., Schweyen R., Larsson C., Suomalainen A.
    FEBS Lett. 494:79-84(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Pituitary.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain, Eye and Salivary gland.

Entry informationi

Entry nameiMFRN2_HUMAN
AccessioniPrimary (citable) accession number: Q96A46
Secondary accession number(s): Q4VBZ0
, Q5T777, Q86VX5, Q969G8, Q9H2J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: December 1, 2001
Last modified: March 16, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.