Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SPRY domain-containing SOCS box protein 4

Gene

SPSB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 4
Short name:
SSB-4
Gene namesi
Name:SPSB4
Synonyms:SSB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:30630. SPSB4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670874.

Polymorphism and mutation databases

BioMutaiSPSB4.
DMDMi74731085.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273SPRY domain-containing SOCS box protein 4PRO_0000238477Add
BLAST

Proteomic databases

PaxDbiQ96A44.
PRIDEiQ96A44.

Expressioni

Gene expression databases

BgeeiQ96A44.
CleanExiHS_SPSB4.
ExpressionAtlasiQ96A44. baseline and differential.
GenevisibleiQ96A44. HS.

Interactioni

Subunit structurei

Interacts with PARW (By similarity). Probable component of the ECS(SPSB4) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB4. Interacts with MET.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TCEB1Q153692EBI-2323233,EBI-301231

Protein-protein interaction databases

BioGridi124941. 8 interactions.
IntActiQ96A44. 6 interactions.
STRINGi9606.ENSP00000311609.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 426Combined sources
Helixi48 – 536Combined sources
Beta strandi55 – 617Combined sources
Beta strandi65 – 684Combined sources
Beta strandi71 – 777Combined sources
Beta strandi81 – 9010Combined sources
Beta strandi93 – 10311Combined sources
Helixi106 – 1083Combined sources
Beta strandi114 – 1185Combined sources
Beta strandi125 – 1295Combined sources
Beta strandi139 – 1435Combined sources
Turni144 – 1474Combined sources
Beta strandi148 – 1503Combined sources
Beta strandi159 – 1624Combined sources
Beta strandi175 – 1828Combined sources
Turni183 – 1864Combined sources
Beta strandi187 – 1926Combined sources
Beta strandi195 – 2017Combined sources
Beta strandi209 – 2157Combined sources
Beta strandi217 – 2193Combined sources
Beta strandi221 – 23010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V24X-ray2.20A28-233[»]
ProteinModelPortaliQ96A44.
SMRiQ96A44. Positions 33-233.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ96A44.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 233200B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST
Domaini234 – 27340SOCS boxPROSITE-ProRule annotationAdd
BLAST

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.By similarity

Sequence similaritiesi

Belongs to the SPSB family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiQ96A44.
KOiK10343.
OMAiCKNQPGV.
OrthoDBiEOG72C513.
PhylomeDBiQ96A44.
TreeFamiTF312822.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q96A44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKLSGSLK SVEVREPALR PAKRELRGAE PGRPARLDQL LDMPAAGLAV
60 70 80 90 100
QLRHAWNPED RSLNVFVKDD DRLTFHRHPV AQSTDGIRGK VGHARGLHAW
110 120 130 140 150
QINWPARQRG THAVVGVATA RAPLHSVGYT ALVGSDAESW GWDLGRSRLY
160 170 180 190 200
HDGKNQPGVA YPAFLGPDEA FALPDSLLVV LDMDEGTLSF IVDGQYLGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EVTMRYINGL DPEPLPLMDL CRRSIRSALG
260 270
RQRLQDISSL PLPQSLKNYL QYQ
Length:273
Mass (Da):30,179
Last modified:December 1, 2001 - v1
Checksum:i234A651A08217489
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403029 mRNA. Translation: AAL57348.1.
AK056367 mRNA. Translation: BAB71165.1.
BC008324 mRNA. Translation: AAH08324.1.
CCDSiCCDS3115.1.
RefSeqiNP_543138.1. NM_080862.2.
XP_011511615.1. XM_011513313.1.
UniGeneiHs.655112.

Genome annotation databases

EnsembliENST00000310546; ENSP00000311609; ENSG00000175093.
GeneIDi92369.
KEGGihsa:92369.
UCSCiuc003ett.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403029 mRNA. Translation: AAL57348.1.
AK056367 mRNA. Translation: BAB71165.1.
BC008324 mRNA. Translation: AAH08324.1.
CCDSiCCDS3115.1.
RefSeqiNP_543138.1. NM_080862.2.
XP_011511615.1. XM_011513313.1.
UniGeneiHs.655112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V24X-ray2.20A28-233[»]
ProteinModelPortaliQ96A44.
SMRiQ96A44. Positions 33-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124941. 8 interactions.
IntActiQ96A44. 6 interactions.
STRINGi9606.ENSP00000311609.

Polymorphism and mutation databases

BioMutaiSPSB4.
DMDMi74731085.

Proteomic databases

PaxDbiQ96A44.
PRIDEiQ96A44.

Protocols and materials databases

DNASUi92369.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310546; ENSP00000311609; ENSG00000175093.
GeneIDi92369.
KEGGihsa:92369.
UCSCiuc003ett.4. human.

Organism-specific databases

CTDi92369.
GeneCardsiSPSB4.
HGNCiHGNC:30630. SPSB4.
MIMi611660. gene.
neXtProtiNX_Q96A44.
PharmGKBiPA142670874.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG055793.
InParanoidiQ96A44.
KOiK10343.
OMAiCKNQPGV.
OrthoDBiEOG72C513.
PhylomeDBiQ96A44.
TreeFamiTF312822.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiSPSB4. human.
EvolutionaryTraceiQ96A44.
GenomeRNAii92369.
NextBioi77732.
PROiQ96A44.
SOURCEiSearch...

Gene expression databases

BgeeiQ96A44.
CleanExiHS_SPSB4.
ExpressionAtlasiQ96A44. baseline and differential.
GenevisibleiQ96A44. HS.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SOCS box proteins."
    Friedel E.J., Nicholson S.E., Nicola N.A., Kile B.T., Hilton D.J.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases."
    Kamura T., Maenaka K., Kotoshiba S., Matsumoto M., Kohda D., Conaway R.C., Conaway J.W., Nakayama K.I.
    Genes Dev. 18:3055-3065(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AN E3 UBIQUITIN-PROTEIN LIGASE COMPLEX, INTERACTION WITH CUL5; RNF7; TCEB1 AND TCEB2.
  5. "The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts with MET and enhances the hepatocyte growth factor-induced Erk-Elk-1-serum response element pathway."
    Wang D., Li Z., Messing E.M., Wu G.
    J. Biol. Chem. 280:16393-16401(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MET.

Entry informationi

Entry nameiSPSB4_HUMAN
AccessioniPrimary (citable) accession number: Q96A44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.