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Protein

Myosin regulatory light chain 2, skeletal muscle isoform

Gene

MYLPF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi38 – 4912Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, skeletal muscle isoform
Alternative name(s):
Fast skeletal myosin light chain 2
MLC2B
Gene namesi
Name:MYLPFBy similarity
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:29824. MYLPF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • lysosomal membrane Source: UniProtKB
  • muscle myosin complex Source: ProtInc
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164723287.

Polymorphism and mutation databases

BioMutaiMYLPF.
DMDMi74760696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 169168Myosin regulatory light chain 2, skeletal muscle isoformBy similarityPRO_0000283746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N,N-trimethylalanineBy similarity
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei25 – 251PhosphothreonineBy similarity
Modified residuei35 – 351PhosphothreonineBy similarity
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei101 – 1011PhosphothreonineBy similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ96A32.
PRIDEiQ96A32.

PTM databases

iPTMnetiQ96A32.
PhosphoSiteiQ96A32.

Expressioni

Tissue specificityi

Expressed in fetal and adult skeletal muscle.1 Publication

Gene expression databases

BgeeiQ96A32.
ExpressionAtlasiQ96A32. baseline and differential.
GenevisibleiQ96A32. HS.

Organism-specific databases

HPAiHPA039262.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

BioGridi118947. 4 interactions.
IntActiQ96A32. 2 interactions.
STRINGi9606.ENSP00000325239.

Structurei

3D structure databases

ProteinModelPortaliQ96A32.
SMRiQ96A32. Positions 17-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 6036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini95 – 13036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini131 – 16636EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ96A32.
KOiK12758.
PhylomeDBiQ96A32.
TreeFamiTF314218.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL
60 70 80 90 100
RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI
110 120 130 140 150
TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN
160
VDYKNICYVI THGDAKDQE
Length:169
Mass (Da):19,015
Last modified:December 1, 2001 - v1
Checksum:i2CE585D6B1F8769D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111V → VA in CAA40762 (PubMed:14756420).Curated
Sequence conflicti11 – 111V → VA in AAA91848 (Ref. 2) Curated
Sequence conflicti169 – 1691E → D in CAG33244 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57543 mRNA. Translation: CAA40762.1.
M21812 mRNA. Translation: AAA91848.1.
AF363061 mRNA. Translation: AAK52797.1.
CR456963 mRNA. Translation: CAG33244.1.
AK314082 mRNA. Translation: BAG36780.1.
CH471192 Genomic DNA. Translation: EAW52254.1.
BC012571 mRNA. Translation: AAH12571.1.
CCDSiCCDS10677.1.
RefSeqiNP_037424.2. NM_013292.3.
UniGeneiHs.50889.

Genome annotation databases

EnsembliENST00000322861; ENSP00000325239; ENSG00000180209.
GeneIDi29895.
KEGGihsa:29895.
UCSCiuc002dxv.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57543 mRNA. Translation: CAA40762.1.
M21812 mRNA. Translation: AAA91848.1.
AF363061 mRNA. Translation: AAK52797.1.
CR456963 mRNA. Translation: CAG33244.1.
AK314082 mRNA. Translation: BAG36780.1.
CH471192 Genomic DNA. Translation: EAW52254.1.
BC012571 mRNA. Translation: AAH12571.1.
CCDSiCCDS10677.1.
RefSeqiNP_037424.2. NM_013292.3.
UniGeneiHs.50889.

3D structure databases

ProteinModelPortaliQ96A32.
SMRiQ96A32. Positions 17-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118947. 4 interactions.
IntActiQ96A32. 2 interactions.
STRINGi9606.ENSP00000325239.

PTM databases

iPTMnetiQ96A32.
PhosphoSiteiQ96A32.

Polymorphism and mutation databases

BioMutaiMYLPF.
DMDMi74760696.

Proteomic databases

PaxDbiQ96A32.
PRIDEiQ96A32.

Protocols and materials databases

DNASUi29895.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322861; ENSP00000325239; ENSG00000180209.
GeneIDi29895.
KEGGihsa:29895.
UCSCiuc002dxv.2. human.

Organism-specific databases

CTDi29895.
GeneCardsiMYLPF.
HGNCiHGNC:29824. MYLPF.
HPAiHPA039262.
neXtProtiNX_Q96A32.
PharmGKBiPA164723287.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ96A32.
KOiK12758.
PhylomeDBiQ96A32.
TreeFamiTF314218.

Enzyme and pathway databases

ReactomeiR-HSA-445355. Smooth Muscle Contraction.

Miscellaneous databases

ChiTaRSiMYLPF. human.
GenomeRNAii29895.
NextBioi52456.
PROiQ96A32.

Gene expression databases

BgeeiQ96A32.
ExpressionAtlasiQ96A32. baseline and differential.
GenevisibleiQ96A32. HS.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human fast skeletal myosin light chain 2 cDNA: isolation, tissue specific expression of the single copy gene, comparative sequence analysis of isoforms and evolutionary relationships."
    Sachdev S., Raychowdhury M.K., Sarkar S.
    DNA Seq. 14:339-350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Skeletal muscleImported.
  2. "Characterization of a full length human skeletal fast light chain 2 cDNA."
    Wu Q.L.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscleImported.
  3. "Cloning and characterization of a human MRLC2 gene encoding a myosin regulatory light chain."
    Gong L., Wang Y.-G., Li T., Wu K.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: PlacentaImported.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tongue.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscleImported.

Entry informationi

Entry nameiMLRS_HUMAN
AccessioniPrimary (citable) accession number: Q96A32
Secondary accession number(s): B2RA83, Q14843, Q6IB41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 1, 2001
Last modified: March 16, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This chain binds calcium.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.