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Protein

Myosin regulatory light chain 2, skeletal muscle isoform

Gene

MYLPF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Miscellaneous

This chain binds calcium.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi38 – 49Sequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMotor protein, Muscle protein, Myosin
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-445355. Smooth Muscle Contraction.
SIGNORiQ96A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, skeletal muscle isoform
Alternative name(s):
Fast skeletal myosin light chain 2
MLC2B
Gene namesi
Name:MYLPFBy similarity
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:29824. MYLPF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • lysosomal membrane Source: UniProtKB
  • muscle myosin complex Source: ProtInc

Pathology & Biotechi

Organism-specific databases

DisGeNETi29895.
OpenTargetsiENSG00000180209.
PharmGKBiPA164723287.

Polymorphism and mutation databases

BioMutaiMYLPF.
DMDMi74760696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002837462 – 169Myosin regulatory light chain 2, skeletal muscle isoformBy similarityAdd BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylalanineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei25PhosphothreonineBy similarity1
Modified residuei35PhosphothreonineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei101PhosphothreonineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ96A32.
PeptideAtlasiQ96A32.
PRIDEiQ96A32.

PTM databases

iPTMnetiQ96A32.
PhosphoSitePlusiQ96A32.

Expressioni

Tissue specificityi

Expressed in fetal and adult skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000180209.
ExpressionAtlasiQ96A32. baseline and differential.
GenevisibleiQ96A32. HS.

Organism-specific databases

HPAiHPA039262.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

BioGridi118947. 5 interactors.
IntActiQ96A32. 2 interactors.
STRINGi9606.ENSP00000325239.

Structurei

3D structure databases

ProteinModelPortaliQ96A32.
SMRiQ96A32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 60EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini95 – 130EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini131 – 166EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ96A32.
KOiK12758.
PhylomeDBiQ96A32.
TreeFamiTF314218.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF13405. EF-hand_6. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 2 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q96A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL
60 70 80 90 100
RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI
110 120 130 140 150
TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN
160
VDYKNICYVI THGDAKDQE
Length:169
Mass (Da):19,015
Last modified:December 1, 2001 - v1
Checksum:i2CE585D6B1F8769D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11V → VA in CAA40762 (PubMed:14756420).Curated1
Sequence conflicti11V → VA in AAA91848 (Ref. 2) Curated1
Sequence conflicti169E → D in CAG33244 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57543 mRNA. Translation: CAA40762.1.
M21812 mRNA. Translation: AAA91848.1.
AF363061 mRNA. Translation: AAK52797.1.
CR456963 mRNA. Translation: CAG33244.1.
AK314082 mRNA. Translation: BAG36780.1.
CH471192 Genomic DNA. Translation: EAW52254.1.
BC012571 mRNA. Translation: AAH12571.1.
CCDSiCCDS10677.1.
RefSeqiNP_001311387.1. NM_001324458.1.
NP_001311388.1. NM_001324459.1.
NP_037424.2. NM_013292.4.
UniGeneiHs.50889.

Genome annotation databases

EnsembliENST00000322861; ENSP00000325239; ENSG00000180209.
GeneIDi29895.
KEGGihsa:29895.
UCSCiuc002dxv.2. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMLRS_HUMAN
AccessioniPrimary (citable) accession number: Q96A32
Secondary accession number(s): B2RA83, Q14843, Q6IB41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 1, 2001
Last modified: May 10, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot