Galactose-3-O-sulfotransferase 3 - Homo sapiens (Human)

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Q96A11 (G3ST3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (6) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Galactose-3-O-sulfotransferase 3
Short name=Gal3ST-3
EC=2.8.2.-

Alternative name(s):
Beta-galactose-3-O-sulfotransferase 3
Short name=Gal3ST3
Gal-beta-1, 3-GalNAc 3'-sulfotransferase 3

Gene names

Name:

GAL3ST3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers a sulfate to position 3 of non-reducing beta-galactosyl residues in N-glycans and core2-branched O-glycans. Has high activity towards Gal-beta-1,4-GlcNAc, Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc and lower activity towards Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc. Ref.1 Ref.2
Cofactor	Magnesium.
Pathway	Protein modification; carbohydrate sulfation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Highly expressed in thyroid, brain, kidney, heart and spinal cord. Ref.1 Ref.2
Sequence similarities	Belongs to the galactose-3-O-sulfotransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0-8.0.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Magnesium
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	monosaccharide metabolic process Non-traceable author statement Ref.2. Source: UniProtKB oligosaccharide metabolic process Non-traceable author statement Ref.2 Ref.1. Source: UniProtKB poly-N-acetyllactosamine metabolic process Non-traceable author statement Ref.2 Ref.1. Source: UniProtKB proteoglycan biosynthetic process Non-traceable author statement Ref.1. Source: UniProtKB sulfur compound metabolic process Non-traceable author statement Ref.2. Source: UniProtKB
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.2. Source: UniProtKB
Molecular_function	3'-phosphoadenosine 5'-phosphosulfate binding Non-traceable author statement Ref.2. Source: UniProtKB carbohydrate binding Non-traceable author statement Ref.2. Source: UniProtKB galactose 3-O-sulfotransferase activity Inferred from direct assay Ref.2. Source: UniProtKB galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: InterPro proteoglycan sulfotransferase activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 431

431

Galactose-3-O-sulfotransferase 3

PRO_0000085206

Regions

Topological domain

1 – 19

Cytoplasmic Potential

Transmembrane

20 – 40

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

41 – 431

391

Lumenal Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

110

N-linked (GlcNAc...) Potential

Glycosylation

177

N-linked (GlcNAc...) Potential

Glycosylation

302

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

221

A → D.
Corresponds to variant rs35285455 [ dbSNP | Ensembl ].

VAR_053988

Natural variant

410

E → A.
Corresponds to variant rs4565902 [ dbSNP | Ensembl ].

VAR_053989

Sequences

Sequence

Length

Mass (Da)

Tools

Q96A11 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 3E1B8418A72A5EB6
Show »

FASTA

431

48,956

        10         20         30         40         50         60 
MPPILQRLQQ ATKMMSRRKI LLLVLGCSTV SLLIHQGAQL SWYPKLFPLS CPPLRNSPPR 

        70         80         90        100        110        120 
PKHMTVAFLK THKTAGTTVQ NILFRFAERH NLTVALPHPS CEHQFCYPRN FSAHFVHPAT 

       130        140        150        160        170        180 
RPPHVLASHL RFDRAELERL MPPSTVYVTI LREPAAMFES LFSYYNQYCP AFRRVPNASL 

       190        200        210        220        230        240 
EAFLRAPEAY YRAGEHFAMF AHNTLAYDLG GDNERSPRDD AAYLAGLIRQ VEEVFSLVMI 

       250        260        270        280        290        300 
AEYFDESLVL LRRLLAWDLD DVLYAKLNAR AASSRLAAIP AALARAARTW NALDAGLYDH 

       310        320        330        340        350        360 
FNATFWRHVA RAGRACVERE ARELREARQR LLRRCFGDEP LLRPAAQIRT KQLQPWQPSR 

       370        380        390        400        410        420 
KVDIMGYDLP GGGAGPATEA CLKLAMPEVQ YSNYLLRKQK RRGGARARPE PVLDNPPPRP 

       430 
IRVLPRGPQG P

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human Gal-3-O-sulfotransferase: molecular cloning, characterization, and its implications in biosynthesis of (SO4-3)Galbeta 1-4(Fucalpha 1-3)GlcNAc." El-Fasakhany F.M., Uchimura K., Kannagi R., Muramatsu T. J. Biol. Chem. 276:26988-26994(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Molecular cloning and expression of a novel human beta-Gal-3-O-sulfotransferase that acts preferentially on N-acetyllactosamine in N- and O-glycans." Suzuki A., Hiraoka N., Suzuki M., Angata K., Misra A.K., McAuliffe J., Hindsgaul O., Fukuda M. J. Biol. Chem. 276:24388-24395(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB053232 mRNA. Translation: BAB61900.1. AY026481 mRNA. Translation: AAK01945.1. BC113552 mRNA. Translation: AAI13553.1. BC113554 mRNA. Translation: AAI13555.1.
IPI	IPI00059164.
RefSeq	NP_149025.1. NM_033036.2.
UniGene	Hs.208343.
3D structure databases
ProteinModelPortal	Q96A11.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000308591.
PTM databases
PhosphoSite	Q96A11.
Polymorphism databases
DMDM	47116425.
Proteomic databases
PaxDb	Q96A11.
PRIDE	Q96A11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312006; ENSP00000308591; ENSG00000175229. ENST00000527878; ENSP00000434829; ENSG00000175229.
GeneID	89792.
KEGG	hsa:89792.
UCSC	uc001ogv.3. human.
Organism-specific databases
CTD	89792.
GeneCards	GC11M065810.
HGNC	HGNC:24144. GAL3ST3.
HPA	HPA013570.
MIM	608234. gene.
neXtProt	NX_Q96A11.
PharmGKB	PA134864180.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42801.
HOGENOM	HOG000232148.
HOVERGEN	HBG051681.
InParanoid	Q96A11.
KO	K09676.
OMA	DHFNATF.
OrthoDB	EOG42BX8P.
PhylomeDB	Q96A11.
Enzyme and pathway databases
UniPathway	UPA00353.
Gene expression databases
Bgee	Q96A11.
CleanEx	HS_GAL3ST3.
Genevestigator	Q96A11.
GermOnline	ENSG00000175229. Homo sapiens.
Family and domain databases
InterPro	IPR009729. Gal-3-0_sulfotransfrase. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR14647. PTHR14647. 1 hit.
Pfam	PF06990. Gal-3-0_sulfotr. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	89792.
NextBio	76281.
SOURCE	Search...

Entry information

Entry name

G3ST3_HUMAN

Accession

Primary (citable) accession number: Q96A11
Secondary accession number(s): Q14D05

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents