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Protein

Protein phosphatase 1 regulatory subunit 14A

Gene

PPP1R14A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-5625740. RHO GTPases activate PKNs.
SIGNORiQ96A00.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14A
Alternative name(s):
17 kDa PKC-potentiated inhibitory protein of PP1
Protein kinase C-potentiated inhibitor protein of 17 kDa
Short name:
CPI-17
Gene namesi
Name:PPP1R14A
Synonyms:CPI17, PPP1INL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:14871. PPP1R14A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33623.

Polymorphism and mutation databases

BioMutaiPPP1R14A.
DMDMi55583974.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Protein phosphatase 1 regulatory subunit 14APRO_0000071486Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei38 – 381Phosphothreonine; by PKC1 Publication
Modified residuei128 – 1281PhosphoserineCombined sources
Modified residuei134 – 1341PhosphoserineCombined sources
Modified residuei136 – 1361PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ96A00.
PaxDbiQ96A00.
PeptideAtlasiQ96A00.
PRIDEiQ96A00.

PTM databases

iPTMnetiQ96A00.
PhosphoSiteiQ96A00.

Expressioni

Tissue specificityi

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.1 Publication

Gene expression databases

BgeeiQ96A00.
CleanExiHS_PPP1R14A.
ExpressionAtlasiQ96A00. baseline and differential.
GenevisibleiQ96A00. HS.

Organism-specific databases

HPAiCAB005044.
CAB009803.
HPA042097.

Interactioni

Protein-protein interaction databases

BioGridi125153. 15 interactions.
IntActiQ96A00. 1 interaction.
MINTiMINT-240329.
STRINGi9606.ENSP00000301242.

Structurei

3D structure databases

ProteinModelPortaliQ96A00.
SMRiQ96A00. Positions 22-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 12086InhibitoryAdd
BLAST

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG410IZE6. Eukaryota.
ENOG4111Q1J. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ96A00.
KOiK12328.
OMAiWIDERLE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ96A00.
TreeFamiTF105546.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q96A00-1) [UniParc]FASTAAdd to basket

Also known as: CPI-17alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL
60 70 80 90 100
DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC
110 120 130 140
GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
Length:147
Mass (Da):16,693
Last modified:December 1, 2001 - v1
Checksum:i470EE2C082FF7319
GO
Isoform 2 (identifier: Q96A00-2) [UniParc]FASTAAdd to basket

Also known as: CPI-17beta

The sequence of this isoform differs from the canonical sequence as follows:
     68-94: Missing.

Show »
Length:120
Mass (Da):13,480
Checksum:iB729738ABCE93AC5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231G → R in AAP97242 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei68 – 9427Missing in isoform 2. 1 PublicationVSP_011841Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056508 mRNA. Translation: BAB62502.1.
AB056509 mRNA. Translation: BAB62503.1.
AF123763 mRNA. Translation: AAP97242.1.
AY050668 Genomic DNA. Translation: AAL24445.1.
AY050670 mRNA. Translation: AAL25828.1.
BC021089 mRNA. Translation: AAH21089.1.
CCDSiCCDS12509.1. [Q96A00-1]
CCDS58660.1. [Q96A00-2]
PIRiJC7719.
RefSeqiNP_001230876.1. NM_001243947.1. [Q96A00-2]
NP_150281.1. NM_033256.2. [Q96A00-1]
UniGeneiHs.631569.

Genome annotation databases

EnsembliENST00000301242; ENSP00000301242; ENSG00000167641. [Q96A00-1]
ENST00000347262; ENSP00000301243; ENSG00000167641. [Q96A00-2]
GeneIDi94274.
KEGGihsa:94274.
UCSCiuc002ohq.3. human. [Q96A00-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056508 mRNA. Translation: BAB62502.1.
AB056509 mRNA. Translation: BAB62503.1.
AF123763 mRNA. Translation: AAP97242.1.
AY050668 Genomic DNA. Translation: AAL24445.1.
AY050670 mRNA. Translation: AAL25828.1.
BC021089 mRNA. Translation: AAH21089.1.
CCDSiCCDS12509.1. [Q96A00-1]
CCDS58660.1. [Q96A00-2]
PIRiJC7719.
RefSeqiNP_001230876.1. NM_001243947.1. [Q96A00-2]
NP_150281.1. NM_033256.2. [Q96A00-1]
UniGeneiHs.631569.

3D structure databases

ProteinModelPortaliQ96A00.
SMRiQ96A00. Positions 22-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125153. 15 interactions.
IntActiQ96A00. 1 interaction.
MINTiMINT-240329.
STRINGi9606.ENSP00000301242.

PTM databases

iPTMnetiQ96A00.
PhosphoSiteiQ96A00.

Polymorphism and mutation databases

BioMutaiPPP1R14A.
DMDMi55583974.

Proteomic databases

MaxQBiQ96A00.
PaxDbiQ96A00.
PeptideAtlasiQ96A00.
PRIDEiQ96A00.

Protocols and materials databases

DNASUi94274.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301242; ENSP00000301242; ENSG00000167641. [Q96A00-1]
ENST00000347262; ENSP00000301243; ENSG00000167641. [Q96A00-2]
GeneIDi94274.
KEGGihsa:94274.
UCSCiuc002ohq.3. human. [Q96A00-1]

Organism-specific databases

CTDi94274.
GeneCardsiPPP1R14A.
HGNCiHGNC:14871. PPP1R14A.
HPAiCAB005044.
CAB009803.
HPA042097.
MIMi608153. gene.
neXtProtiNX_Q96A00.
PharmGKBiPA33623.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZE6. Eukaryota.
ENOG4111Q1J. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ96A00.
KOiK12328.
OMAiWIDERLE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ96A00.
TreeFamiTF105546.

Enzyme and pathway databases

ReactomeiR-HSA-5625740. RHO GTPases activate PKNs.
SIGNORiQ96A00.

Miscellaneous databases

ChiTaRSiPPP1R14A. human.
GeneWikiiPPP1R14A.
GenomeRNAii94274.
PROiQ96A00.
SOURCEiSearch...

Gene expression databases

BgeeiQ96A00.
CleanExiHS_PPP1R14A.
ExpressionAtlasiQ96A00. baseline and differential.
GenevisibleiQ96A00. HS.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Aorta.
  2. "Cloning a new human cDNA homologous to Sus scrofa mRNA for 17-KDa PKC-potentiated inhibitory protein of PP1."
    Cui W.C., Yu L., Zhao S.Y.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA."
    Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J., Uhl G.R.
    Biochem. J. 377:171-181(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Tissue: Smooth muscle.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas.
  5. "Association of CPI-17 with protein kinase C and casein kinase I."
    Zemlickova E., Johannes F.J., Aitken A., Dubois T.
    Biochem. Biophys. Res. Commun. 316:39-47(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-38.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-128; SER-134 AND SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPP14A_HUMAN
AccessioniPrimary (citable) accession number: Q96A00
Secondary accession number(s): Q7Z4X7, Q96S54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.