Q96A00 (PP14A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 14A

Alternative name(s):
17 kDa PKC-potentiated inhibitory protein of PP1
Protein kinase C-potentiated inhibitor protein of 17 kDa
Short name=CPI-17

Gene names

Name:	PPP1R14A
Synonyms:	CPI17, PPP1INL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. Ref.1
Sequence similarities	Belongs to the PP1 inhibitor family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Protein phosphatase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein phosphatase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96A00-1)

Also known as: CPI-17alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96A00-2)

Also known as: CPI-17beta;

The sequence of this isoform differs from the canonical sequence as follows:
     68-94: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Protein phosphatase 1 regulatory subunit 14A		PRO_0000071486
Regions
	Region	35 – 120	86	Inhibitory
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.5 Ref.6
	Modified residue	38	1	Phosphothreonine By similarity
	Modified residue	128	1	Phosphoserine Ref.5 Ref.6
	Modified residue	134	1	Phosphoserine Ref.6
	Modified residue	136	1	Phosphoserine Ref.5 Ref.6
Natural variations
	Alternative sequence	68 – 94	27	Missing in isoform 2.		VSP_011841
Experimental info
	Sequence conflict	123	1	G → R in AAP97242. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CPI-17alpha) [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 470EE2C082FF7319	FASTA	147	16,693
	10 20 30 40 50 60 MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL 70 80 90 100 110 120 EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR 130 140 QPGLRQPSPS HDGSLSPLQD RARTAHP
	Isoform 2 (CPI-17beta) [UniParc]. Checksum: B729738ABCE93AC5	FASTA	120	13,480
	10 20 30 40 50 60 MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL 70 80 90 100 110 120 EELYRGMGLL KSCGKPVEDF IQELLAKLQG LHRQPGLRQP SPSHDGSLSP LQDRARTAHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase." Yamawaki K., Ito M., Machida H., Moriki N., Okamoto R., Isaka N., Shimpo H., Kohda A., Okumura K., Hartshorne D.J., Nakano T. Biochem. Biophys. Res. Commun. 285:1040-1045(2001) [PubMed: 11467857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Aorta.
[2]	"Cloning a new human cDNA homologous to Sus scrofa mRNA for 17-KDa PKC-potentiated inhibitory protein of PP1." Cui W.C., Yu L., Zhao S.Y. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA." Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J., Uhl G.R. Biochem. J. 377:171-181(2004) [PubMed: 12974676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Tissue: Smooth muscle.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[5]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-128 AND SER-136, MASS SPECTROMETRY. Tissue: Platelet.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-128; SER-134 AND SER-136, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB056508 mRNA. Translation: BAB62502.1. AB056509 mRNA. Translation: BAB62503.1. AF123763 mRNA. Translation: AAP97242.1. AY050668 Genomic DNA. Translation: AAL24445.1. AY050670 mRNA. Translation: AAL25828.1. BC021089 mRNA. Translation: AAH21089.1.
IPI	IPI00045503. IPI00059135.
PIR	JC7719.
RefSeq	NP_001230876.1. NM_001243947.1. NP_150281.1. NM_033256.2.
UniGene	Hs.631569.
3D structure databases
ProteinModelPortal	Q96A00.
SMR	Q96A00. Positions 22-120.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q96A00. 1 interaction.
MINT	MINT-240329.
STRING	Q96A00.
PTM databases
PhosphoSite	Q96A00.
Polymorphism databases
DMDM	55583974.
Proteomic databases
PRIDE	Q96A00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301242; ENSP00000301242; ENSG00000167641.
GeneID	94274.
KEGG	hsa:94274.
UCSC	uc002ohq.1. human. uc010efv.1. human.
Organism-specific databases
CTD	94274.
GeneCards	GC19M038741.
H-InvDB	HIX0015086.
HGNC	HGNC:14871. PPP1R14A.
HPA	CAB005044. CAB009803.
MIM	608153. gene.
neXtProt	NX_Q96A00.
PharmGKB	PA33623.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21083.
GeneTree	ENSGT00390000003608.
HOGENOM	HBG717795.
HOVERGEN	HBG054419.
InParanoid	Q96A00.
OMA	PIEDFIQ.
OrthoDB	EOG4HDSW5.
PhylomeDB	Q96A00.
Gene expression databases
ArrayExpress	Q96A00.
Bgee	Q96A00.
CleanEx	HS_PPP1R14A.
Genevestigator	Q96A00.
GermOnline	ENSG00000167641. Homo sapiens.
Family and domain databases
InterPro	IPR008025. PP1_inhibitor. [Graphical view]
Gene3D	G3DSA:1.10.150.220. PP1_inhibitor. 1 hit.
KO	K12328.
PANTHER	PTHR16188. PP1_inhibitor. 1 hit.
Pfam	PF05361. PP1_inhibitor. 1 hit. [Graphical view]
SUPFAM	SSF81790. PP1_inhibitor. 1 hit.
ProtoNet	Search...
Other
NextBio	78503.
SOURCE	Search...

Entry information

Entry name

PP14A_HUMAN

Accession

Primary (citable) accession number: Q96A00
Secondary accession number(s): Q7Z4X7, Q96S54

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.