ID   FAKD4_HUMAN             Reviewed;         631 AA.
AC   Q969Z0; A4D2L2; A4D2L3; D3DVL5; D3DVL6; O14710; Q53GI8; Q8NDM4; Q9BUC6;
AC   Q9Y2F6;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 172.
DE   RecName: Full=FAST kinase domain-containing protein 4 {ECO:0000303|PubMed:20869947};
DE   AltName: Full=Cell cycle progression restoration protein 2;
DE            Short=Cell cycle progression protein 2;
DE   AltName: Full=Protein TBRG4;
DE   AltName: Full=Transforming growth factor beta regulator 4 {ECO:0000312|HGNC:HGNC:17443};
DE   Flags: Precursor;
GN   Name=TBRG4 {ECO:0000312|HGNC:HGNC:17443};
GN   Synonyms=CPR2 {ECO:0000303|PubMed:9383053}, FASTKD4
GN   {ECO:0000303|PubMed:20869947}, KIAA0948;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=10231032; DOI=10.1093/dnares/6.1.63;
RA   Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA   Tanaka A., Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XIII. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 6:63-70(1999).
RN   [2]
RP   SEQUENCE REVISION.
RA   Ohara O., Nagase T., Kikuno R.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Kidney;
RA   Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA   Tanaka A., Yokoyama S.;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS SER-22 AND
RP   LEU-57.
RC   TISSUE=Mammary cancer;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA   Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA   Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA   Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA   Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA   Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA   Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA   Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA   Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA   Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA   Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA   Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA   Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Lung, and Uterus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 172-615 (ISOFORM 1), AND FUNCTION.
RC   TISSUE=Hepatoma;
RX   PubMed=9383053; DOI=10.1093/genetics/147.3.1063;
RA   Edwards M.C., Liegeois N., Horecka J., DePinho R.A., Sprague G.F. Jr.,
RA   Tyers M., Elledge S.J.;
RT   "Human CPR (cell cycle progression restoration) genes impart a Far-
RT   phenotype on yeast cells.";
RL   Genetics 147:1063-1076(1997).
RN   [9]
RP   TISSUE SPECIFICITY.
RC   TISSUE=Lung;
RX   PubMed=12759187; DOI=10.1016/s0167-4781(03)00051-4;
RA   Rival-Gervier S., Thepot D., Jolivet G., Houdebine L.-M.;
RT   "Pig whey acidic protein gene is surrounded by two ubiquitously expressed
RT   genes.";
RL   Biochim. Biophys. Acta 1627:7-14(2003).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA   Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA   Greff Z., Keri G., Stemmann O., Mann M.;
RT   "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT   kinome across the cell cycle.";
RL   Mol. Cell 31:438-448(2008).
RN   [11]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19608861; DOI=10.1126/science.1175371;
RA   Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA   Olsen J.V., Mann M.;
RT   "Lysine acetylation targets protein complexes and co-regulates major
RT   cellular functions.";
RL   Science 325:834-840(2009).
RN   [12]
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=20869947; DOI=10.1016/j.bbrc.2010.09.075;
RA   Simarro M., Gimenez-Cassina A., Kedersha N., Lazaro J.B., Adelmant G.O.,
RA   Marto J.A., Rhee K., Tisdale S., Danial N., Benarafa C., Orduna A.,
RA   Anderson P.;
RT   "Fast kinase domain-containing protein 3 is a mitochondrial protein
RT   essential for cellular respiration.";
RL   Biochem. Biophys. Res. Commun. 401:440-446(2010).
RN   [13]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [14]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [15]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-553, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [16]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
RN   [17]
RP   SUBCELLULAR LOCATION, FUNCTION, DOMAIN, AND MUTAGENESIS OF ASP-531.
RX   PubMed=28335001; DOI=10.1093/nar/gkx164;
RA   Boehm E., Zaganelli S., Maundrell K., Jourdain A.A., Thore S.,
RA   Martinou J.C.;
RT   "FASTKD1 and FASTKD4 have opposite effects on expression of specific
RT   mitochondrial RNAs, depending upon their endonuclease-like RAP domain.";
RL   Nucleic Acids Res. 45:6135-6146(2017).
CC   -!- FUNCTION: Plays a role in processing of mitochondrial RNA precursors
CC       and in stabilization of a subset of mature mitochondrial RNA species,
CC       such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6.
CC       May play a role in cell cycle progression (PubMed:9383053).
CC       {ECO:0000269|PubMed:28335001, ECO:0000269|PubMed:9383053}.
CC   -!- INTERACTION:
CC       Q969Z0; Q6RW13: AGTRAP; NbExp=3; IntAct=EBI-702328, EBI-741181;
CC       Q969Z0; Q6RW13-2: AGTRAP; NbExp=3; IntAct=EBI-702328, EBI-11522760;
CC       Q969Z0; Q8TD16-2: BICD2; NbExp=3; IntAct=EBI-702328, EBI-11975051;
CC       Q969Z0; Q8WZ55: BSND; NbExp=3; IntAct=EBI-702328, EBI-7996695;
CC       Q969Z0; Q13137: CALCOCO2; NbExp=3; IntAct=EBI-702328, EBI-739580;
CC       Q969Z0; Q86X02: CDR2L; NbExp=3; IntAct=EBI-702328, EBI-11063830;
CC       Q969Z0; Q8IZR5-2: CMTM4; NbExp=3; IntAct=EBI-702328, EBI-17278014;
CC       Q969Z0; Q96DZ9-2: CMTM5; NbExp=3; IntAct=EBI-702328, EBI-11522780;
CC       Q969Z0; Q9NRY5: FAM114A2; NbExp=4; IntAct=EBI-702328, EBI-10973142;
CC       Q969Z0; Q8IZU0: FAM9B; NbExp=3; IntAct=EBI-702328, EBI-10175124;
CC       Q969Z0; Q96D09: GPRASP2; NbExp=3; IntAct=EBI-702328, EBI-473189;
CC       Q969Z0; Q7Z5P4: HSD17B13; NbExp=3; IntAct=EBI-702328, EBI-18053395;
CC       Q969Z0; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-702328, EBI-3044087;
CC       Q969Z0; Q9H400: LIME1; NbExp=3; IntAct=EBI-702328, EBI-2830566;
CC       Q969Z0; Q969L2: MAL2; NbExp=3; IntAct=EBI-702328, EBI-944295;
CC       Q969Z0; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-702328, EBI-16439278;
CC       Q969Z0; Q04118: PRB3; NbExp=3; IntAct=EBI-702328, EBI-13360404;
CC       Q969Z0; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-702328, EBI-10192441;
CC       Q969Z0; O15126: SCAMP1; NbExp=3; IntAct=EBI-702328, EBI-954338;
CC       Q969Z0; Q8WV19: SFT2D1; NbExp=3; IntAct=EBI-702328, EBI-2854842;
CC       Q969Z0; B7ZLI8: STK19; NbExp=3; IntAct=EBI-702328, EBI-10176124;
CC       Q969Z0; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-702328, EBI-1105213;
CC       Q969Z0; Q8WW34-2: TMEM239; NbExp=3; IntAct=EBI-702328, EBI-11528917;
CC       Q969Z0; Q9UBN6: TNFRSF10D; NbExp=3; IntAct=EBI-702328, EBI-1044859;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC       {ECO:0000269|PubMed:20869947, ECO:0000269|PubMed:28335001}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q969Z0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q969Z0-2; Sequence=VSP_022460;
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed (PubMed:12759187).
CC       Expression detected in spleen, thymus, testis, ovary, colon, heart,
CC       smooth muscle, kidney, brain, lung, liver and white adipose tissue with
CC       highest expression in smooth muscle (PubMed:20869947).
CC       {ECO:0000269|PubMed:12759187, ECO:0000269|PubMed:20869947}.
CC   -!- DOMAIN: RAP domain is required for TBRG4 function in mRNA stability and
CC       translation. {ECO:0000269|PubMed:28335001}.
CC   -!- SIMILARITY: Belongs to the FAST kinase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB69312.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAA76792.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB023165; BAA76792.2; ALT_INIT; mRNA.
DR   EMBL; AK222943; BAD96663.1; -; mRNA.
DR   EMBL; AL833840; CAD38700.1; -; mRNA.
DR   EMBL; CH236960; EAL23744.1; -; Genomic_DNA.
DR   EMBL; CH236960; EAL23745.1; -; Genomic_DNA.
DR   EMBL; CH471128; EAW61051.1; -; Genomic_DNA.
DR   EMBL; CH471128; EAW61052.1; -; Genomic_DNA.
DR   EMBL; CH471128; EAW61054.1; -; Genomic_DNA.
DR   EMBL; CH471128; EAW61055.1; -; Genomic_DNA.
DR   EMBL; BC002732; AAH02732.2; -; mRNA.
DR   EMBL; BC014918; AAH14918.1; -; mRNA.
DR   EMBL; BC017235; AAH17235.1; -; mRNA.
DR   EMBL; AF011792; AAB69312.1; ALT_FRAME; mRNA.
DR   CCDS; CCDS5501.1; -. [Q969Z0-1]
DR   CCDS; CCDS5502.1; -. [Q969Z0-2]
DR   RefSeq; NP_004740.2; NM_004749.3. [Q969Z0-1]
DR   RefSeq; NP_112162.1; NM_030900.3. [Q969Z0-2]
DR   RefSeq; NP_954573.1; NM_199122.2. [Q969Z0-2]
DR   AlphaFoldDB; Q969Z0; -.
DR   SMR; Q969Z0; -.
DR   BioGRID; 114666; 417.
DR   IntAct; Q969Z0; 64.
DR   MINT; Q969Z0; -.
DR   STRING; 9606.ENSP00000258770; -.
DR   GlyGen; Q969Z0; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q969Z0; -.
DR   MetOSite; Q969Z0; -.
DR   PhosphoSitePlus; Q969Z0; -.
DR   SwissPalm; Q969Z0; -.
DR   BioMuta; TBRG4; -.
DR   DMDM; 74731072; -.
DR   EPD; Q969Z0; -.
DR   jPOST; Q969Z0; -.
DR   MassIVE; Q969Z0; -.
DR   MaxQB; Q969Z0; -.
DR   PaxDb; 9606-ENSP00000258770; -.
DR   PeptideAtlas; Q969Z0; -.
DR   ProteomicsDB; 75880; -. [Q969Z0-1]
DR   ProteomicsDB; 75881; -. [Q969Z0-2]
DR   Pumba; Q969Z0; -.
DR   Antibodypedia; 13534; 203 antibodies from 28 providers.
DR   DNASU; 9238; -.
DR   Ensembl; ENST00000258770.8; ENSP00000258770.3; ENSG00000136270.14. [Q969Z0-1]
DR   Ensembl; ENST00000361278.7; ENSP00000354992.3; ENSG00000136270.14. [Q969Z0-2]
DR   Ensembl; ENST00000395655.8; ENSP00000379016.4; ENSG00000136270.14. [Q969Z0-2]
DR   Ensembl; ENST00000494076.5; ENSP00000420597.1; ENSG00000136270.14. [Q969Z0-1]
DR   GeneID; 9238; -.
DR   KEGG; hsa:9238; -.
DR   MANE-Select; ENST00000258770.8; ENSP00000258770.3; NM_004749.4; NP_004740.2.
DR   UCSC; uc003tmv.5; human. [Q969Z0-1]
DR   AGR; HGNC:17443; -.
DR   CTD; 9238; -.
DR   DisGeNET; 9238; -.
DR   GeneCards; TBRG4; -.
DR   HGNC; HGNC:17443; TBRG4.
DR   HPA; ENSG00000136270; Low tissue specificity.
DR   MIM; 611325; gene.
DR   neXtProt; NX_Q969Z0; -.
DR   OpenTargets; ENSG00000136270; -.
DR   PharmGKB; PA134882196; -.
DR   VEuPathDB; HostDB:ENSG00000136270; -.
DR   eggNOG; ENOG502QTRE; Eukaryota.
DR   GeneTree; ENSGT01030000234607; -.
DR   HOGENOM; CLU_029448_0_0_1; -.
DR   InParanoid; Q969Z0; -.
DR   OMA; NFNHSQV; -.
DR   OrthoDB; 5347066at2759; -.
DR   PhylomeDB; Q969Z0; -.
DR   TreeFam; TF324885; -.
DR   PathwayCommons; Q969Z0; -.
DR   Reactome; R-HSA-9837092; FASTK family proteins regulate processing and stability of mitochondrial RNAs.
DR   SignaLink; Q969Z0; -.
DR   BioGRID-ORCS; 9238; 49 hits in 1161 CRISPR screens.
DR   ChiTaRS; TBRG4; human.
DR   GeneWiki; TBRG4; -.
DR   GenomeRNAi; 9238; -.
DR   Pharos; Q969Z0; Tbio.
DR   PRO; PR:Q969Z0; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q969Z0; Protein.
DR   Bgee; ENSG00000136270; Expressed in mucosa of transverse colon and 131 other cell types or tissues.
DR   ExpressionAtlas; Q969Z0; baseline and differential.
DR   GO; GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR   GO; GO:0035770; C:ribonucleoprotein granule; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR   GO; GO:0090615; P:mitochondrial mRNA processing; IMP:UniProtKB.
DR   GO; GO:0000963; P:mitochondrial RNA processing; IBA:GO_Central.
DR   GO; GO:0016071; P:mRNA metabolic process; IMP:UniProtKB.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; TAS:UniProtKB.
DR   GO; GO:0051726; P:regulation of cell cycle; TAS:UniProtKB.
DR   GO; GO:0044528; P:regulation of mitochondrial mRNA stability; IMP:UniProtKB.
DR   InterPro; IPR013579; FAST_2.
DR   InterPro; IPR010622; FAST_Leu-rich.
DR   InterPro; IPR013584; RAP.
DR   PANTHER; PTHR21228:SF59; FAST KINASE DOMAIN-CONTAINING PROTEIN 4; 1.
DR   PANTHER; PTHR21228; FAST LEU-RICH DOMAIN-CONTAINING; 1.
DR   Pfam; PF06743; FAST_1; 1.
DR   Pfam; PF08368; FAST_2; 1.
DR   Pfam; PF08373; RAP; 1.
DR   SMART; SM00952; RAP; 1.
DR   PROSITE; PS51286; RAP; 1.
DR   Genevisible; Q969Z0; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Mitochondrion; Phosphoprotein; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..107
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           108..631
FT                   /note="FAST kinase domain-containing protein 4"
FT                   /id="PRO_0000273026"
FT   DOMAIN          561..619
FT                   /note="RAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
FT   MOD_RES         553
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VAR_SEQ         246..355
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10231032, ECO:0000303|Ref.3"
FT                   /id="VSP_022460"
FT   VARIANT         22
FT                   /note="A -> S (in dbSNP:rs2304694)"
FT                   /evidence="ECO:0000269|PubMed:17974005"
FT                   /id="VAR_030071"
FT   VARIANT         57
FT                   /note="P -> L (in dbSNP:rs2304693)"
FT                   /evidence="ECO:0000269|PubMed:17974005"
FT                   /id="VAR_030072"
FT   MUTAGEN         531
FT                   /note="D->A: Does not affect location in mitochondria;
FT                   fails to rescue the increased accumulation of the level of
FT                   mature MT-ND3, MT-CO3, MT-CYB or MT-ND5 mRNA in
FT                   TBRG4-deficient cells; does not abolish the accumulation of
FT                   the MT-ND5-CYB precursor RNA in a TBRG4-deficient cell
FT                   line."
FT                   /evidence="ECO:0000269|PubMed:28335001"
FT   CONFLICT        365
FT                   /note="T -> A (in Ref. 3; BAD96663)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        489
FT                   /note="V -> E (in Ref. 8; AAB69312)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        524
FT                   /note="A -> S (in Ref. 8; AAB69312)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   631 AA;  70738 MW;  4EFC3AF5D5BBABD3 CRC64;
     MAAHLVKRCT CLLREAARQA PAMAPVGRLR LAWVAHKTLT SSATSPISHL PGSLMEPVEK
     ERASTPYIEK QVDHLIKKAT RPEELLELLG GSHDLDSNQA AMVLIRLSHL LSEKPEDKGL
     LIQDAHFHQL LCLLNSQIAS VWHGTLSKLL GSLYALGIPK ASKELQSVEQ EVRWRMRKLK
     YKHLAFLAES CATLSQEQHS QELLAELLTH LERRWTEIED SHTLVTVMMK VGHLSEPLMN
     RLEDKCLELV EHFGPNELRK VLVMLAAQSR RSVPLLRAIS YHLVQKPFSL TKDVLLDVAY
     AYGKLSFHQT QVSQRLATDL LSLMPSLTSG EVAHCAKSFA LLKWLSLPLF EAFAQHVLNR
     AQDITLPHLC SVLLAFARLN FHPDQEDQFF SLVHEKLGSE LPGLEPALQV DLVWALCVLQ
     QAREAELQAV LHPEFHIQFL GGKSQKDQNT FQKLLHINAT ALLEYPEYSG PLLPASAVAP
     GPSALDRKVT PLQKELQETL KGLLGSADKG SLEVATQYGW VLDAEVLLDS DGEFLPVRDF
     VAPHLAQPTG SQSPPPGSKR LAFLRWEFPN FNSRSKDLLG RFVLARRHIV AAGFLIVDVP
     FYEWLELKSE WQKGAYLKDK MRKAVAEELA K
//