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Protein

FAST kinase domain-containing protein 4

Gene

TBRG4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in processing of mitochondrial RNA precursors and in stabilization of a subset of mature mitochondrial RNA species, such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6. May play a role in cell cycle progression (PubMed:9383053).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell cycle arrest Source: UniProtKB
  • cellular respiration Source: UniProtKB
  • cellular response to calcium ion Source: ARUK-UCL
  • mitochondrial mRNA processing Source: UniProtKB
  • mRNA metabolic process Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • regulation of mitochondrial mRNA stability Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
FAST kinase domain-containing protein 41 Publication
Alternative name(s):
Cell cycle progression restoration protein 2
Short name:
Cell cycle progression protein 2
Protein TBRG4
Transforming growth factor beta regulator 4Imported
Gene namesi
Name:TBRG4Imported
Synonyms:CPR21 Publication, FASTKD41 Publication, KIAA0948
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136270.13
HGNCiHGNC:17443 TBRG4
MIMi611325 gene
neXtProtiNX_Q969Z0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi531D → A: Does not affect location in mitochondria; fails to rescue the increased accumulation of the level of mature MT-ND3, MT-CO3, MT-CYB or MT-ND5 mRNA in TBRG4-deficient cells; does not abolish the accumulation of the MT-ND5-CYB precursor RNA in a TBRG4-deficient cell line. 1 Publication1

Organism-specific databases

DisGeNETi9238
OpenTargetsiENSG00000136270
PharmGKBiPA134882196

Polymorphism and mutation databases

BioMutaiTBRG4
DMDMi74731072

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 107MitochondrionSequence analysisAdd BLAST107
ChainiPRO_0000273026108 – 631FAST kinase domain-containing protein 4Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei553PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ969Z0
MaxQBiQ969Z0
PaxDbiQ969Z0
PeptideAtlasiQ969Z0
PRIDEiQ969Z0

PTM databases

iPTMnetiQ969Z0
PhosphoSitePlusiQ969Z0

Expressioni

Tissue specificityi

Ubiquitously expressed (PubMed:12759187). Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in smooth muscle (PubMed:20869947).2 Publications

Gene expression databases

BgeeiENSG00000136270
CleanExiHS_TBRG4
ExpressionAtlasiQ969Z0 baseline and differential
GenevisibleiQ969Z0 HS

Organism-specific databases

HPAiHPA020582
HPA050430

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114666, 41 interactors
IntActiQ969Z0, 22 interactors
MINTiQ969Z0
STRINGi9606.ENSP00000258770

Structurei

3D structure databases

ProteinModelPortaliQ969Z0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini561 – 619RAPPROSITE-ProRule annotationAdd BLAST59

Domaini

RAP domain is required for TBRG4 function in mRNA stability and translation.1 Publication

Sequence similaritiesi

Belongs to the FAST kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IEJA Eukaryota
ENOG4111EYX LUCA
GeneTreeiENSGT00530000063266
HOVERGENiHBG094028
InParanoidiQ969Z0
OMAiMMKVGHL
OrthoDBiEOG091G0WV6
PhylomeDBiQ969Z0
TreeFamiTF324885

Family and domain databases

InterProiView protein in InterPro
IPR013579 FAST_2
IPR010622 FAST_Leu-rich
IPR013584 RAP
PfamiView protein in Pfam
PF06743 FAST_1, 1 hit
PF08368 FAST_2, 1 hit
PF08373 RAP, 1 hit
SMARTiView protein in SMART
SM00952 RAP, 1 hit
PROSITEiView protein in PROSITE
PS51286 RAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969Z0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAHLVKRCT CLLREAARQA PAMAPVGRLR LAWVAHKTLT SSATSPISHL
60 70 80 90 100
PGSLMEPVEK ERASTPYIEK QVDHLIKKAT RPEELLELLG GSHDLDSNQA
110 120 130 140 150
AMVLIRLSHL LSEKPEDKGL LIQDAHFHQL LCLLNSQIAS VWHGTLSKLL
160 170 180 190 200
GSLYALGIPK ASKELQSVEQ EVRWRMRKLK YKHLAFLAES CATLSQEQHS
210 220 230 240 250
QELLAELLTH LERRWTEIED SHTLVTVMMK VGHLSEPLMN RLEDKCLELV
260 270 280 290 300
EHFGPNELRK VLVMLAAQSR RSVPLLRAIS YHLVQKPFSL TKDVLLDVAY
310 320 330 340 350
AYGKLSFHQT QVSQRLATDL LSLMPSLTSG EVAHCAKSFA LLKWLSLPLF
360 370 380 390 400
EAFAQHVLNR AQDITLPHLC SVLLAFARLN FHPDQEDQFF SLVHEKLGSE
410 420 430 440 450
LPGLEPALQV DLVWALCVLQ QAREAELQAV LHPEFHIQFL GGKSQKDQNT
460 470 480 490 500
FQKLLHINAT ALLEYPEYSG PLLPASAVAP GPSALDRKVT PLQKELQETL
510 520 530 540 550
KGLLGSADKG SLEVATQYGW VLDAEVLLDS DGEFLPVRDF VAPHLAQPTG
560 570 580 590 600
SQSPPPGSKR LAFLRWEFPN FNSRSKDLLG RFVLARRHIV AAGFLIVDVP
610 620 630
FYEWLELKSE WQKGAYLKDK MRKAVAEELA K
Length:631
Mass (Da):70,738
Last modified:December 1, 2001 - v1
Checksum:i4EFC3AF5D5BBABD3
GO
Isoform 2 (identifier: Q969Z0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-355: Missing.

Show »
Length:521
Mass (Da):58,443
Checksum:iCE2A279B0BFEE5B6
GO

Sequence cautioni

The sequence AAB69312 differs from that shown. Reason: Frameshift at positions 211, 479, 498 and 576.Curated
The sequence BAA76792 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti365T → A in BAD96663 (Ref. 3) Curated1
Sequence conflicti489V → E in AAB69312 (PubMed:9383053).Curated1
Sequence conflicti524A → S in AAB69312 (PubMed:9383053).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03007122A → S1 PublicationCorresponds to variant dbSNP:rs2304694Ensembl.1
Natural variantiVAR_03007257P → L1 PublicationCorresponds to variant dbSNP:rs2304693Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022460246 – 355Missing in isoform 2. 2 PublicationsAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023165 mRNA Translation: BAA76792.2 Different initiation.
AK222943 mRNA Translation: BAD96663.1
AL833840 mRNA Translation: CAD38700.1
CH236960 Genomic DNA Translation: EAL23744.1
CH236960 Genomic DNA Translation: EAL23745.1
CH471128 Genomic DNA Translation: EAW61051.1
CH471128 Genomic DNA Translation: EAW61052.1
CH471128 Genomic DNA Translation: EAW61054.1
CH471128 Genomic DNA Translation: EAW61055.1
BC002732 mRNA Translation: AAH02732.2
BC014918 mRNA Translation: AAH14918.1
BC017235 mRNA Translation: AAH17235.1
AF011792 mRNA Translation: AAB69312.1 Frameshift.
CCDSiCCDS5501.1 [Q969Z0-1]
CCDS5502.1 [Q969Z0-2]
RefSeqiNP_004740.2, NM_004749.3 [Q969Z0-1]
NP_112162.1, NM_030900.3 [Q969Z0-2]
NP_954573.1, NM_199122.2 [Q969Z0-2]
UniGeneiHs.231411
Hs.741611

Genome annotation databases

EnsembliENST00000258770; ENSP00000258770; ENSG00000136270 [Q969Z0-1]
ENST00000361278; ENSP00000354992; ENSG00000136270 [Q969Z0-2]
ENST00000395655; ENSP00000379016; ENSG00000136270 [Q969Z0-2]
ENST00000494076; ENSP00000420597; ENSG00000136270 [Q969Z0-1]
GeneIDi9238
KEGGihsa:9238
UCSCiuc003tmv.5 human [Q969Z0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiFAKD4_HUMAN
AccessioniPrimary (citable) accession number: Q969Z0
Secondary accession number(s): A4D2L2
, A4D2L3, D3DVL5, D3DVL6, O14710, Q53GI8, Q8NDM4, Q9BUC6, Q9Y2F6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health