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Q969X5

- ERGI1_HUMAN

UniProt

Q969X5 - ERGI1_HUMAN

Protein

Endoplasmic reticulum-Golgi intermediate compartment protein 1

Gene

ERGIC1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Possible role in transport between endoplasmic reticulum and Golgi.1 Publication

    GO - Molecular functioni

    1. protein binding Source: HGNC

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: HGNC

    Keywords - Biological processi

    ER-Golgi transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmic reticulum-Golgi intermediate compartment protein 1
    Alternative name(s):
    ER-Golgi intermediate compartment 32 kDa protein
    Short name:
    ERGIC-32
    Gene namesi
    Name:ERGIC1
    Synonyms:ERGIC32, KIAA1181Imported
    ORF Names:HT034
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:29205. ERGIC1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication. Endoplasmic reticulum-Golgi intermediate compartment membrane 1 Publication; Multi-pass membrane protein 1 Publication. Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Cycles between the endoplasmic reticulum and the Golgi.

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: HGNC
    2. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. Golgi membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW
    6. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA143485456.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Endoplasmic reticulum-Golgi intermediate compartment protein 1PRO_0000087023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)2 Publications

    Post-translational modificationi

    N-glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ969X5.
    PaxDbiQ969X5.
    PRIDEiQ969X5.

    PTM databases

    PhosphoSiteiQ969X5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ969X5.
    BgeeiQ969X5.
    CleanExiHS_ERGIC1.
    GenevestigatoriQ969X5.

    Organism-specific databases

    HPAiHPA018666.
    HPA018900.

    Interactioni

    Subunit structurei

    Interacts with ERGIC3/ERV46.1 Publication

    Protein-protein interaction databases

    BioGridi121458. 20 interactions.
    IntActiQ969X5. 2 interactions.
    MINTiMINT-5000068.
    STRINGi9606.ENSP00000377374.

    Structurei

    3D structure databases

    ProteinModelPortaliQ969X5.
    SMRiQ969X5. Positions 114-240.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2626CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini48 – 254207LumenalSequence AnalysisAdd
    BLAST
    Topological domaini276 – 29015CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei27 – 4721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ERGIC family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG260258.
    HOGENOMiHOG000210954.
    HOVERGENiHBG081479.
    InParanoidiQ969X5.
    OMAiHEYILKI.
    OrthoDBiEOG7TJ3JS.
    PhylomeDBiQ969X5.
    TreeFamiTF354253.

    Family and domain databases

    InterProiIPR012936. Erv_C.
    [Graphical view]
    PfamiPF07970. COPIIcoated_ERV. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q969X5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPFDFRRFDI YRKVPKDLTQ PTYTGAIISI CCCLFILFLF LSELTGFITT    50
    EVVNELYVDD PDKDSGGKID VSLNISLPNL HCELVGLDIQ DEMGRHEVGH 100
    IDNSMKIPLN NGAGCRFEGQ FSINKVPGNF HVSTHSATAQ PQNPDMTHVI 150
    HKLSFGDTLQ VQNIHGAFNA LGGADRLTSN PLASHDYILK IVPTVYEDKS 200
    GKQRYSYQYT VANKEYVAYS HTGRIIPAIW FRYDLSPITV KYTERRQPLY 250
    RFITTICAII GGTFTVAGIL DSCIFTASEA WKKIQLGKMH 290
    Length:290
    Mass (Da):32,592
    Last modified:December 1, 2001 - v1
    Checksum:i5B13C7B8B9F8E51D
    GO
    Isoform 2 (identifier: Q969X5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-92: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:198
    Mass (Da):22,160
    Checksum:i931E3EBE28377777
    GO
    Isoform 3 (identifier: Q969X5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-45: Missing.
         46-52: GFITTEV → MERWAMR
         127-204: PGNFHVSTHS...VYEDKSGKQR → WKPCLSPFYL...GSGRSYSSLQ
         205-290: Missing.

    Show »
    Length:159
    Mass (Da):17,778
    Checksum:iFB02DEBA58B1D20D
    GO

    Sequence cautioni

    The sequence AAG44724.1 differs from that shown. Reason: Frameshift at positions 100 and 150.
    The sequence BAA86495.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9292Missing in isoform 2. 1 PublicationVSP_011560Add
    BLAST
    Alternative sequencei1 – 4545Missing in isoform 3. 2 PublicationsVSP_011561Add
    BLAST
    Alternative sequencei46 – 527GFITTEV → MERWAMR in isoform 3. 2 PublicationsVSP_011562
    Alternative sequencei127 – 20478PGNFH…SGKQR → WKPCLSPFYLLPFPAVSPLP GNWLWRHSLDLTLTQPPASE GSCPAAWPFLLRIWMGVQAP WGFKPLMAGSGRSYSSLQ in isoform 3. 2 PublicationsVSP_011563Add
    BLAST
    Alternative sequencei205 – 29086Missing in isoform 3. 2 PublicationsVSP_011564Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB033007 mRNA. Translation: BAA86495.1. Different initiation.
    AF267855 mRNA. Translation: AAG44724.1. Frameshift.
    AL136753 mRNA. Translation: CAB66687.1.
    CR533474 mRNA. Translation: CAG38505.1.
    BC012766 mRNA. Translation: AAH12766.1.
    BC014490 mRNA. Translation: AAH14490.1.
    CCDSiCCDS34292.1. [Q969X5-1]
    RefSeqiNP_001026881.1. NM_001031711.2. [Q969X5-1]
    XP_006714955.1. XM_006714892.1. [Q969X5-2]
    XP_006714957.1. XM_006714894.1. [Q969X5-3]
    UniGeneiHs.509163.

    Genome annotation databases

    EnsembliENST00000326654; ENSP00000325127; ENSG00000113719. [Q969X5-3]
    ENST00000393784; ENSP00000377374; ENSG00000113719. [Q969X5-1]
    GeneIDi57222.
    KEGGihsa:57222.
    UCSCiuc003mbw.4. human. [Q969X5-1]
    uc003mbz.4. human. [Q969X5-3]

    Polymorphism databases

    DMDMi51701446.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB033007 mRNA. Translation: BAA86495.1 . Different initiation.
    AF267855 mRNA. Translation: AAG44724.1 . Frameshift.
    AL136753 mRNA. Translation: CAB66687.1 .
    CR533474 mRNA. Translation: CAG38505.1 .
    BC012766 mRNA. Translation: AAH12766.1 .
    BC014490 mRNA. Translation: AAH14490.1 .
    CCDSi CCDS34292.1. [Q969X5-1 ]
    RefSeqi NP_001026881.1. NM_001031711.2. [Q969X5-1 ]
    XP_006714955.1. XM_006714892.1. [Q969X5-2 ]
    XP_006714957.1. XM_006714894.1. [Q969X5-3 ]
    UniGenei Hs.509163.

    3D structure databases

    ProteinModelPortali Q969X5.
    SMRi Q969X5. Positions 114-240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121458. 20 interactions.
    IntActi Q969X5. 2 interactions.
    MINTi MINT-5000068.
    STRINGi 9606.ENSP00000377374.

    PTM databases

    PhosphoSitei Q969X5.

    Polymorphism databases

    DMDMi 51701446.

    Proteomic databases

    MaxQBi Q969X5.
    PaxDbi Q969X5.
    PRIDEi Q969X5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000326654 ; ENSP00000325127 ; ENSG00000113719 . [Q969X5-3 ]
    ENST00000393784 ; ENSP00000377374 ; ENSG00000113719 . [Q969X5-1 ]
    GeneIDi 57222.
    KEGGi hsa:57222.
    UCSCi uc003mbw.4. human. [Q969X5-1 ]
    uc003mbz.4. human. [Q969X5-3 ]

    Organism-specific databases

    CTDi 57222.
    GeneCardsi GC05P172261.
    H-InvDB HIX0005421.
    HGNCi HGNC:29205. ERGIC1.
    HPAi HPA018666.
    HPA018900.
    neXtProti NX_Q969X5.
    PharmGKBi PA143485456.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG260258.
    HOGENOMi HOG000210954.
    HOVERGENi HBG081479.
    InParanoidi Q969X5.
    OMAi HEYILKI.
    OrthoDBi EOG7TJ3JS.
    PhylomeDBi Q969X5.
    TreeFami TF354253.

    Miscellaneous databases

    ChiTaRSi ERGIC1. human.
    GenomeRNAii 57222.
    NextBioi 63349.
    PROi Q969X5.

    Gene expression databases

    ArrayExpressi Q969X5.
    Bgeei Q969X5.
    CleanExi HS_ERGIC1.
    Genevestigatori Q969X5.

    Family and domain databases

    InterProi IPR012936. Erv_C.
    [Graphical view ]
    Pfami PF07970. COPIIcoated_ERV. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
      Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
      DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: BrainImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Hypothalamus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Testis.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: LymphImported and PancreasImported.
    6. "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46."
      Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P.
      J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 177-199 AND 233-241, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION, INTERACTION WITH ERGIC3.
      Tissue: Hepatoma1 Publication.
    7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74.
      Tissue: Liver.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiERGI1_HUMAN
    AccessioniPrimary (citable) accession number: Q969X5
    Secondary accession number(s): Q9H0L0, Q9H2J2, Q9ULN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3