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Q969X5

- ERGI1_HUMAN

UniProt

Q969X5 - ERGI1_HUMAN

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Protein

Endoplasmic reticulum-Golgi intermediate compartment protein 1

Gene

ERGIC1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Possible role in transport between endoplasmic reticulum and Golgi.1 Publication

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: HGNC
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum-Golgi intermediate compartment protein 1
Alternative name(s):
ER-Golgi intermediate compartment 32 kDa protein
Short name:
ERGIC-32
Gene namesi
Name:ERGIC1
Synonyms:ERGIC32, KIAA1181Imported
ORF Names:HT034
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:29205. ERGIC1.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication. Endoplasmic reticulum-Golgi intermediate compartment membrane 1 Publication; Multi-pass membrane protein 1 Publication. Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Cycles between the endoplasmic reticulum and the Golgi.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. endoplasmic reticulum-Golgi intermediate compartment Source: HGNC
  3. Golgi apparatus Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485456.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Endoplasmic reticulum-Golgi intermediate compartment protein 1PRO_0000087023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)2 Publications

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ969X5.
PaxDbiQ969X5.
PRIDEiQ969X5.

PTM databases

PhosphoSiteiQ969X5.

Expressioni

Gene expression databases

BgeeiQ969X5.
CleanExiHS_ERGIC1.
ExpressionAtlasiQ969X5. baseline and differential.
GenevestigatoriQ969X5.

Organism-specific databases

HPAiHPA018666.
HPA018900.

Interactioni

Subunit structurei

Interacts with ERGIC3/ERV46.1 Publication

Protein-protein interaction databases

BioGridi121458. 23 interactions.
IntActiQ969X5. 2 interactions.
MINTiMINT-5000068.
STRINGi9606.ENSP00000377374.

Structurei

3D structure databases

ProteinModelPortaliQ969X5.
SMRiQ969X5. Positions 114-240.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini48 – 254207LumenalSequence AnalysisAdd
BLAST
Topological domaini276 – 29015CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei27 – 4721HelicalSequence AnalysisAdd
BLAST
Transmembranei255 – 27521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the ERGIC family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG260258.
GeneTreeiENSGT00530000063113.
HOGENOMiHOG000210954.
HOVERGENiHBG081479.
InParanoidiQ969X5.
OMAiHEYILKI.
OrthoDBiEOG7TJ3JS.
PhylomeDBiQ969X5.
TreeFamiTF354253.

Family and domain databases

InterProiIPR012936. Erv_C.
[Graphical view]
PfamiPF07970. COPIIcoated_ERV. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q969X5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFDFRRFDI YRKVPKDLTQ PTYTGAIISI CCCLFILFLF LSELTGFITT
60 70 80 90 100
EVVNELYVDD PDKDSGGKID VSLNISLPNL HCELVGLDIQ DEMGRHEVGH
110 120 130 140 150
IDNSMKIPLN NGAGCRFEGQ FSINKVPGNF HVSTHSATAQ PQNPDMTHVI
160 170 180 190 200
HKLSFGDTLQ VQNIHGAFNA LGGADRLTSN PLASHDYILK IVPTVYEDKS
210 220 230 240 250
GKQRYSYQYT VANKEYVAYS HTGRIIPAIW FRYDLSPITV KYTERRQPLY
260 270 280 290
RFITTICAII GGTFTVAGIL DSCIFTASEA WKKIQLGKMH
Length:290
Mass (Da):32,592
Last modified:December 1, 2001 - v1
Checksum:i5B13C7B8B9F8E51D
GO
Isoform 2 (identifier: Q969X5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.

Note: No experimental confirmation available.

Show »
Length:198
Mass (Da):22,160
Checksum:i931E3EBE28377777
GO
Isoform 3 (identifier: Q969X5-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
     46-52: GFITTEV → MERWAMR
     127-204: PGNFHVSTHS...VYEDKSGKQR → WKPCLSPFYL...GSGRSYSSLQ
     205-290: Missing.

Show »
Length:159
Mass (Da):17,778
Checksum:iFB02DEBA58B1D20D
GO

Sequence cautioni

The sequence AAG44724.1 differs from that shown. Reason: Frameshift at positions 100 and 150.
The sequence BAA86495.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9292Missing in isoform 2. 1 PublicationVSP_011560Add
BLAST
Alternative sequencei1 – 4545Missing in isoform 3. 2 PublicationsVSP_011561Add
BLAST
Alternative sequencei46 – 527GFITTEV → MERWAMR in isoform 3. 2 PublicationsVSP_011562
Alternative sequencei127 – 20478PGNFH…SGKQR → WKPCLSPFYLLPFPAVSPLP GNWLWRHSLDLTLTQPPASE GSCPAAWPFLLRIWMGVQAP WGFKPLMAGSGRSYSSLQ in isoform 3. 2 PublicationsVSP_011563Add
BLAST
Alternative sequencei205 – 29086Missing in isoform 3. 2 PublicationsVSP_011564Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB033007 mRNA. Translation: BAA86495.1. Different initiation.
AF267855 mRNA. Translation: AAG44724.1. Frameshift.
AL136753 mRNA. Translation: CAB66687.1.
CR533474 mRNA. Translation: CAG38505.1.
BC012766 mRNA. Translation: AAH12766.1.
BC014490 mRNA. Translation: AAH14490.1.
CCDSiCCDS34292.1. [Q969X5-1]
RefSeqiNP_001026881.1. NM_001031711.2. [Q969X5-1]
XP_006714955.1. XM_006714892.1. [Q969X5-2]
XP_006714957.1. XM_006714894.1. [Q969X5-3]
UniGeneiHs.509163.

Genome annotation databases

EnsembliENST00000326654; ENSP00000325127; ENSG00000113719. [Q969X5-3]
ENST00000393784; ENSP00000377374; ENSG00000113719. [Q969X5-1]
GeneIDi57222.
KEGGihsa:57222.
UCSCiuc003mbw.4. human. [Q969X5-1]
uc003mbz.4. human. [Q969X5-3]

Polymorphism databases

DMDMi51701446.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB033007 mRNA. Translation: BAA86495.1 . Different initiation.
AF267855 mRNA. Translation: AAG44724.1 . Frameshift.
AL136753 mRNA. Translation: CAB66687.1 .
CR533474 mRNA. Translation: CAG38505.1 .
BC012766 mRNA. Translation: AAH12766.1 .
BC014490 mRNA. Translation: AAH14490.1 .
CCDSi CCDS34292.1. [Q969X5-1 ]
RefSeqi NP_001026881.1. NM_001031711.2. [Q969X5-1 ]
XP_006714955.1. XM_006714892.1. [Q969X5-2 ]
XP_006714957.1. XM_006714894.1. [Q969X5-3 ]
UniGenei Hs.509163.

3D structure databases

ProteinModelPortali Q969X5.
SMRi Q969X5. Positions 114-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121458. 23 interactions.
IntActi Q969X5. 2 interactions.
MINTi MINT-5000068.
STRINGi 9606.ENSP00000377374.

PTM databases

PhosphoSitei Q969X5.

Polymorphism databases

DMDMi 51701446.

Proteomic databases

MaxQBi Q969X5.
PaxDbi Q969X5.
PRIDEi Q969X5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000326654 ; ENSP00000325127 ; ENSG00000113719 . [Q969X5-3 ]
ENST00000393784 ; ENSP00000377374 ; ENSG00000113719 . [Q969X5-1 ]
GeneIDi 57222.
KEGGi hsa:57222.
UCSCi uc003mbw.4. human. [Q969X5-1 ]
uc003mbz.4. human. [Q969X5-3 ]

Organism-specific databases

CTDi 57222.
GeneCardsi GC05P172261.
H-InvDB HIX0005421.
HGNCi HGNC:29205. ERGIC1.
HPAi HPA018666.
HPA018900.
neXtProti NX_Q969X5.
PharmGKBi PA143485456.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG260258.
GeneTreei ENSGT00530000063113.
HOGENOMi HOG000210954.
HOVERGENi HBG081479.
InParanoidi Q969X5.
OMAi HEYILKI.
OrthoDBi EOG7TJ3JS.
PhylomeDBi Q969X5.
TreeFami TF354253.

Miscellaneous databases

ChiTaRSi ERGIC1. human.
GenomeRNAii 57222.
NextBioi 63349.
PROi Q969X5.

Gene expression databases

Bgeei Q969X5.
CleanExi HS_ERGIC1.
ExpressionAtlasi Q969X5. baseline and differential.
Genevestigatori Q969X5.

Family and domain databases

InterProi IPR012936. Erv_C.
[Graphical view ]
Pfami PF07970. COPIIcoated_ERV. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
    Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
    DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: BrainImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Hypothalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Testis.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: LymphImported and PancreasImported.
  6. "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46."
    Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P.
    J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 177-199 AND 233-241, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION, INTERACTION WITH ERGIC3.
    Tissue: Hepatoma1 Publication.
  7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74.
    Tissue: Liver.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiERGI1_HUMAN
AccessioniPrimary (citable) accession number: Q969X5
Secondary accession number(s): Q9H0L0, Q9H2J2, Q9ULN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3