Q969X5 (ERGI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoplasmic reticulum-Golgi intermediate compartment protein 1

Alternative name(s):
ER-Golgi intermediate compartment 32 kDa protein
Short name=ERGIC-32

Gene names

Name:	ERGIC1
Synonyms:	ERGIC32, KIAA1181
ORF Names:	HT034

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in transport between endoplasmic reticulum and Golgi. Ref.6
Subunit structure	Interacts with ERGIC3/ERV46. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Cycles between the endoplasmic reticulum and the Golgi. Ref.6
Post-translational modification	N-glycosylated. Ref.6
Sequence similarities	Belongs to the ERGIC family.
Sequence caution	The sequence AAG44724.1 differs from that shown. Reason: Frameshift at positions 100 and 150. The sequence BAA86495.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from direct assay Ref.6. Source: HGNC
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum-Golgi intermediate compartment Inferred from direct assay Ref.6. Source: HGNC endoplasmic reticulum-Golgi intermediate compartment membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERGIC3	Q9Y282	1	EBI-781527,EBI-781551

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q969X5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q969X5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q969X5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
     46-52: GFITTEV → MERWAMR
     127-204: PGNFHVSTHS...VYEDKSGKQR → WKPCLSPFYL...GSGRSYSSLQ
     205-290: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Endoplasmic reticulum-Golgi intermediate compartment protein 1		PRO_0000087023
Regions
	Topological domain	1 – 26	26	Cytoplasmic Potential
	Transmembrane	27 – 47	21	Helical; Potential
	Topological domain	48 – 254	207	Lumenal Potential
	Transmembrane	255 – 275	21	Helical; Potential
	Topological domain	276 – 290	15	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	74	1	N-linked (GlcNAc...) Ref.6 Ref.7
Natural variations
	Alternative sequence	1 – 92	92	Missing in isoform 2.		VSP_011560
	Alternative sequence	1 – 45	45	Missing in isoform 3.		VSP_011561
	Alternative sequence	46 – 52	7	GFITTEV → MERWAMR in isoform 3.		VSP_011562
	Alternative sequence	127 – 204	78	PGNFH…SGKQR → WKPCLSPFYLLPFPAVSPLP GNWLWRHSLDLTLTQPPASE GSCPAAWPFLLRIWMGVQAP WGFKPLMAGSGRSYSSLQ in isoform 3.		VSP_011563
	Alternative sequence	205 – 290	86	Missing in isoform 3.		VSP_011564

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5B13C7B8B9F8E51D	FASTA	290	32,592
	10 20 30 40 50 60 MPFDFRRFDI YRKVPKDLTQ PTYTGAIISI CCCLFILFLF LSELTGFITT EVVNELYVDD 70 80 90 100 110 120 PDKDSGGKID VSLNISLPNL HCELVGLDIQ DEMGRHEVGH IDNSMKIPLN NGAGCRFEGQ 130 140 150 160 170 180 FSINKVPGNF HVSTHSATAQ PQNPDMTHVI HKLSFGDTLQ VQNIHGAFNA LGGADRLTSN 190 200 210 220 230 240 PLASHDYILK IVPTVYEDKS GKQRYSYQYT VANKEYVAYS HTGRIIPAIW FRYDLSPITV 250 260 270 280 290 KYTERRQPLY RFITTICAII GGTFTVAGIL DSCIFTASEA WKKIQLGKMH
	Isoform 2 [UniParc]. Checksum: 931E3EBE28377777	FASTA	198	22,160
	10 20 30 40 50 60 MGRHEVGHID NSMKIPLNNG AGCRFEGQFS INKVPGNFHV STHSATAQPQ NPDMTHVIHK 70 80 90 100 110 120 LSFGDTLQVQ NIHGAFNALG GADRLTSNPL ASHDYILKIV PTVYEDKSGK QRYSYQYTVA 130 140 150 160 170 180 NKEYVAYSHT GRIIPAIWFR YDLSPITVKY TERRQPLYRF ITTICAIIGG TFTVAGILDS 190 CIFTASEAWK KIQLGKMH
	Isoform 3 [UniParc]. Checksum: FB02DEBA58B1D20D	FASTA	159	17,778
	10 20 30 40 50 60 MERWAMRVNE LYVDDPDKDS GGKIDVSLNI SLPNLHCELV GLDIQDEMGR HEVGHIDNSM 70 80 90 100 110 120 KIPLNNGAGC RFEGQFSINK VWKPCLSPFY LLPFPAVSPL PGNWLWRHSL DLTLTQPPAS 130 140 150 EGSCPAAWPF LLRIWMGVQA PWGFKPLMAG SGRSYSSLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain." Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O. DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hypothalamus.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph and Pancreas.
[6]	"Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46." Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P. J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 177-199 AND 233-241, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION, INTERACTION WITH ERGIC3. Tissue: Hepatoma.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB033007 mRNA. Translation: BAA86495.1. Different initiation. AF267855 mRNA. Translation: AAG44724.1. Frameshift. AL136753 mRNA. Translation: CAB66687.1. CR533474 mRNA. Translation: CAG38505.1. BC012766 mRNA. Translation: AAH12766.1. BC014490 mRNA. Translation: AAH14490.1.
IPI	IPI00003635. IPI00021763. IPI00022887.
RefSeq	NP_001026881.1. NM_001031711.2.
UniGene	Hs.509163.
3D structure databases
ProteinModelPortal	Q969X5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969X5. 2 interactions.
STRING	9606.ENSP00000377374.
PTM databases
PhosphoSite	Q969X5.
Polymorphism databases
DMDM	51701446.
Proteomic databases
PaxDb	Q969X5.
PRIDE	Q969X5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000326654; ENSP00000325127; ENSG00000113719. ENST00000393784; ENSP00000377374; ENSG00000113719.
GeneID	57222.
KEGG	hsa:57222.
UCSC	uc003mbw.4. human. uc003mbz.4. human.
Organism-specific databases
CTD	57222.
GeneCards	GC05P172261.
H-InvDB	HIX0005421.
HGNC	HGNC:29205. ERGIC1.
HPA	HPA018666. HPA018900.
neXtProt	NX_Q969X5.
PharmGKB	PA143485456.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG260258.
HOGENOM	HOG000210954.
HOVERGEN	HBG081479.
InParanoid	Q969X5.
OMA	HEYILKI.
OrthoDB	EOG47H5QF.
Gene expression databases
ArrayExpress	Q969X5.
Bgee	Q969X5.
CleanEx	HS_ERGIC1.
Genevestigator	Q969X5.
GermOnline	ENSG00000113719. Homo sapiens.
Family and domain databases
InterPro	IPR012936. Erv_C. [Graphical view]
Pfam	PF07970. COPIIcoated_ERV. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ERGIC1. human.
GenomeRNAi	57222.
NextBio	63349.

Entry information

Entry name

ERGI1_HUMAN

Accession

Primary (citable) accession number: Q969X5
Secondary accession number(s): Q9H0L0, Q9H2J2, Q9ULN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.