Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q969X5 (ERGI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoplasmic reticulum-Golgi intermediate compartment protein 1
Alternative name(s):
ER-Golgi intermediate compartment 32 kDa protein
Short name=ERGIC-32
Gene names
Name:ERGIC1
Synonyms:ERGIC32, KIAA1181
ORF Names:HT034
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible role in transport between endoplasmic reticulum and Golgi. Ref.6

Subunit structure

Interacts with ERGIC3/ERV46. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Cycles between the endoplasmic reticulum and the Golgi. Ref.6

Post-translational modification

N-glycosylated. Ref.6

Sequence similarities

Belongs to the ERGIC family.

Sequence caution

The sequence AAG44724.1 differs from that shown. Reason: Frameshift at positions 100 and 150.

The sequence BAA86495.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q969X5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q969X5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q969X5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
     46-52: GFITTEV → MERWAMR
     127-204: PGNFHVSTHS...VYEDKSGKQR → WKPCLSPFYL...GSGRSYSSLQ
     205-290: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Endoplasmic reticulum-Golgi intermediate compartment protein 1
PRO_0000087023

Regions

Topological domain1 – 2626Cytoplasmic Potential
Transmembrane27 – 4721Helical; Potential
Topological domain48 – 254207Lumenal Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 29015Cytoplasmic Potential

Amino acid modifications

Glycosylation741N-linked (GlcNAc...) Ref.6 Ref.7

Natural variations

Alternative sequence1 – 9292Missing in isoform 2.
VSP_011560
Alternative sequence1 – 4545Missing in isoform 3.
VSP_011561
Alternative sequence46 – 527GFITTEV → MERWAMR in isoform 3.
VSP_011562
Alternative sequence127 – 20478PGNFH…SGKQR → WKPCLSPFYLLPFPAVSPLP GNWLWRHSLDLTLTQPPASE GSCPAAWPFLLRIWMGVQAP WGFKPLMAGSGRSYSSLQ in isoform 3.
VSP_011563
Alternative sequence205 – 29086Missing in isoform 3.
VSP_011564

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5B13C7B8B9F8E51D

FASTA29032,592
        10         20         30         40         50         60 
MPFDFRRFDI YRKVPKDLTQ PTYTGAIISI CCCLFILFLF LSELTGFITT EVVNELYVDD 

        70         80         90        100        110        120 
PDKDSGGKID VSLNISLPNL HCELVGLDIQ DEMGRHEVGH IDNSMKIPLN NGAGCRFEGQ 

       130        140        150        160        170        180 
FSINKVPGNF HVSTHSATAQ PQNPDMTHVI HKLSFGDTLQ VQNIHGAFNA LGGADRLTSN 

       190        200        210        220        230        240 
PLASHDYILK IVPTVYEDKS GKQRYSYQYT VANKEYVAYS HTGRIIPAIW FRYDLSPITV 

       250        260        270        280        290 
KYTERRQPLY RFITTICAII GGTFTVAGIL DSCIFTASEA WKKIQLGKMH 

« Hide

Isoform 2 [UniParc].

Checksum: 931E3EBE28377777
Show »

FASTA19822,160
Isoform 3 [UniParc].

Checksum: FB02DEBA58B1D20D
Show »

FASTA15917,778

References

« Hide 'large scale' references
[1]"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Hypothalamus.
[3]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph and Pancreas.
[6]"Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46."
Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W., Hauri H.-P.
J. Biol. Chem. 279:47242-47253(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 177-199 AND 233-241, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION, INTERACTION WITH ERGIC3.
Tissue: Hepatoma.
[7]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74.
Tissue: Liver.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB033007 mRNA. Translation: BAA86495.1. Different initiation.
AF267855 mRNA. Translation: AAG44724.1. Frameshift.
AL136753 mRNA. Translation: CAB66687.1.
CR533474 mRNA. Translation: CAG38505.1.
BC012766 mRNA. Translation: AAH12766.1.
BC014490 mRNA. Translation: AAH14490.1.
RefSeqNP_001026881.1. NM_001031711.2.
UniGeneHs.509163.

3D structure databases

ProteinModelPortalQ969X5.
SMRQ969X5. Positions 114-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121458. 16 interactions.
IntActQ969X5. 2 interactions.
MINTMINT-5000068.
STRING9606.ENSP00000377374.

PTM databases

PhosphoSiteQ969X5.

Polymorphism databases

DMDM51701446.

Proteomic databases

PaxDbQ969X5.
PRIDEQ969X5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000326654; ENSP00000325127; ENSG00000113719. [Q969X5-3]
ENST00000393784; ENSP00000377374; ENSG00000113719. [Q969X5-1]
GeneID57222.
KEGGhsa:57222.
UCSCuc003mbw.4. human. [Q969X5-1]
uc003mbz.4. human. [Q969X5-3]

Organism-specific databases

CTD57222.
GeneCardsGC05P172261.
H-InvDBHIX0005421.
HGNCHGNC:29205. ERGIC1.
HPAHPA018666.
HPA018900.
neXtProtNX_Q969X5.
PharmGKBPA143485456.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG260258.
HOGENOMHOG000210954.
HOVERGENHBG081479.
InParanoidQ969X5.
OMAHEYILKI.
OrthoDBEOG7TJ3JS.
PhylomeDBQ969X5.
TreeFamTF354253.

Gene expression databases

ArrayExpressQ969X5.
BgeeQ969X5.
CleanExHS_ERGIC1.
GenevestigatorQ969X5.

Family and domain databases

InterProIPR012936. Erv_C.
[Graphical view]
PfamPF07970. COPIIcoated_ERV. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSERGIC1. human.
GenomeRNAi57222.
NextBio63349.
PROQ969X5.

Entry information

Entry nameERGI1_HUMAN
AccessionPrimary (citable) accession number: Q969X5
Secondary accession number(s): Q9H0L0, Q9H2J2, Q9ULN9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM