Q969W9 (PMEPA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane prostate androgen-induced protein

Alternative name(s):
Solid tumor-associated 1 protein

Gene names

Name:	PMEPA1
Synonyms:	STAG1, TMEPAI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Ref.8
Subunit structure	Interacts with NEDD4 (via WW domains). Interacts with AR. Ref.7 Ref.8
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Tissue specificity	Highest expression in prostate. Also expressed in ovary.
Induction	By androgen.
Domain	The WW-binding motifs mediate interaction with NEDD4 By similarity.
Sequence similarities	Belongs to the PMEPA1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen receptor signaling pathway Non-traceable author statement Ref.1. Source: UniProtKB negative regulation of transforming growth factor beta receptor signaling pathway Traceable author statement. Source: Reactome transforming growth factor beta receptor signaling pathway Traceable author statement. Source: Reactome
Cellular_component	early endosome membrane Traceable author statement. Source: Reactome integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q969W9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q969W9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: MHRLMGVNSTAAAAAGQPNVSCTCNCKRSLFQSMEIT → MA

Note: No experimental confirmation available.

Isoform 3 (identifier: Q969W9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Transmembrane prostate androgen-induced protein		PRO_0000185442
Regions
	Topological domain	1 – 40	40	Extracellular Potential
	Transmembrane	41 – 63	23	Helical; Potential
	Topological domain	64 – 287	224	Cytoplasmic Potential
	Motif	158 – 161	4	WW-binding 1
	Motif	229 – 232	4	WW-binding 2
Natural variations
	Alternative sequence	1 – 50	50	Missing in isoform 3.		VSP_044588
	Alternative sequence	1 – 37	37	MHRLM…SMEIT → MA in isoform 2.		VSP_006438
	Natural variant	128	1	E → D. Corresponds to variant rs41314918 [ dbSNP | Ensembl ].		VAR_062154
Experimental info
	Mutagenesis	161	1	Y → A: Impairs interaction with NEDD4. Impairs polyubiquitination of AR; when associated with A-232. Ref.7 Ref.8
	Mutagenesis	232	1	Y → A: Impairs interaction with NEDD4. Impairs polyubiquitination of AR; when associated with A-232. Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6103473561AE08DA	FASTA	287	31,609
	10 20 30 40 50 60 MHRLMGVNST AAAAAGQPNV SCTCNCKRSL FQSMEITELE FVQIIIIVVV MMVMVVVITC 70 80 90 100 110 120 LLSHYKLSAR SFISRHSQGR RREDALSSEG CLWPSESTVS GNGIPEPQVY APPRPTDRLA 130 140 150 160 170 180 VPPFAQRERF HRFQPTYPYL QHEIDLPPTI SLSDGEEPPP YQGPCTLQLR DPEQQLELNR 190 200 210 220 230 240 ESVRAPPNRT IFDSDLMDSA RLGGPCPPSS NSGISATCYG SGGRMEGPPP TYSEVIGHYP 250 260 270 280 GSSFQHQQSS GPPSLLEGTR LHHTHIAPLE SAAIWSKEKD KQKGHPL
	Isoform 2 [UniParc]. Checksum: 0864B9DAB8A21959	FASTA	252	27,900
	10 20 30 40 50 60 MAELEFVQII IIVVVMMVMV VVITCLLSHY KLSARSFISR HSQGRRREDA LSSEGCLWPS 70 80 90 100 110 120 ESTVSGNGIP EPQVYAPPRP TDRLAVPPFA QRERFHRFQP TYPYLQHEID LPPTISLSDG 130 140 150 160 170 180 EEPPPYQGPC TLQLRDPEQQ LELNRESVRA PPNRTIFDSD LMDSARLGGP CPPSSNSGIS 190 200 210 220 230 240 ATCYGSGGRM EGPPPTYSEV IGHYPGSSFQ HQQSSGPPSL LEGTRLHHTH IAPLESAAIW 250 SKEKDKQKGH PL
	Isoform 3 [UniParc]. Checksum: A44A274EAABFD930	FASTA	237	26,201
	10 20 30 40 50 60 MMVMVVVITC LLSHYKLSAR SFISRHSQGR RREDALSSEG CLWPSESTVS GNGIPEPQVY 70 80 90 100 110 120 APPRPTDRLA VPPFAQRERF HRFQPTYPYL QHEIDLPPTI SLSDGEEPPP YQGPCTLQLR 130 140 150 160 170 180 DPEQQLELNR ESVRAPPNRT IFDSDLMDSA RLGGPCPPSS NSGISATCYG SGGRMEGPPP 190 200 210 220 230 TYSEVIGHYP GSSFQHQQSS GPPSLLEGTR LHHTHIAPLE SAAIWSKEKD KQKGHPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel androgen-regulated gene, PMEPA1, located on chromosome 20q13 exhibits high level expression in prostate." Xu L.L., Shanmugam N., Segawa T., Sesterhenn I.A., McLeod D.G., Moul J.W., Srivastava S. Genomics 66:257-263(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors." Rae F.K., Hooper J.D., Nicol D.L., Clements J.A. Mol. Carcinog. 32:44-53(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"PMEPA1, a transforming growth factor-beta-induced marker of terminal colonocyte differentiation whose expression is maintained in primary and metastatic colon cancer." Brunschwig E.B., Wilson K., Mack D., Dawson D., Lawrence E., Willson J.K.V., Lu S., Nosrati A., Rerko R.M., Swinler S., Beard L., Lutterbaugh J.D., Willis J., Platzer P., Markowitz S. Cancer Res. 63:1568-1575(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-287 (ISOFORM 1). Tissue: Kidney.
[7]	"PMEPA1, an androgen-regulated NEDD4-binding protein, exhibits cell growth inhibitory function and decreased expression during prostate cancer progression." Xu L.L., Shi Y., Petrovics G., Sun C., Makarem M., Zhang W., Sesterhenn I.A., McLeod D.G., Sun L., Moul J.W., Srivastava S. Cancer Res. 63:4299-4304(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NEDD4, MUTAGENESIS OF TYR-161 AND TYR-232.
[8]	"A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells." Li H., Xu L.L., Masuda K., Raymundo E., McLeod D.G., Dobi A., Srivastava S. J. Biol. Chem. 283:28988-28995(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AR, MUTAGENESIS OF TYR-161 AND TYR-232.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF224278 mRNA. Translation: AAF86322.1. AF305616 mRNA. Translation: AAL16781.1. AY128643 mRNA. Translation: AAM89277.1. AF305426 Genomic DNA. Translation: AAL09357.1. AL035541, AL121913 Genomic DNA. Translation: CAI23105.1. AL121913, AL035541 Genomic DNA. Translation: CAI19031.1. AL121913, AL035541 Genomic DNA. Translation: CAM27272.1. AL035541, AL121913 Genomic DNA. Translation: CAM28295.1. CH471077 Genomic DNA. Translation: EAW75505.1. CH471077 Genomic DNA. Translation: EAW75508.1. BC015918 mRNA. Translation: AAH15918.2.
IPI	IPI00056521. IPI00183679. IPI00396293.
RefSeq	NP_001242905.1. NM_001255976.1. NP_064567.2. NM_020182.4. NP_954638.1. NM_199169.2. NP_954639.1. NM_199170.2. NP_954640.1. NM_199171.2.
UniGene	Hs.517155.
3D structure databases
ProteinModelPortal	Q969W9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969W9. 1 interaction.
STRING	9606.ENSP00000345826.
PTM databases
PhosphoSite	Q969W9.
Polymorphism databases
DMDM	20140695.
Proteomic databases
PaxDb	Q969W9.
PRIDE	Q969W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265626; ENSP00000265626; ENSG00000124225. ENST00000341744; ENSP00000345826; ENSG00000124225. ENST00000347215; ENSP00000344014; ENSG00000124225. ENST00000395814; ENSP00000379159; ENSG00000124225. ENST00000395816; ENSP00000379161; ENSG00000124225.
GeneID	56937.
KEGG	hsa:56937.
UCSC	uc002xyq.3. human.
Organism-specific databases
CTD	56937.
GeneCards	GC20M056223.
HGNC	HGNC:14107. PMEPA1.
MIM	606564. gene.
neXtProt	NX_Q969W9.
PharmGKB	PA162399822.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG27420.
HOGENOM	HOG000276535.
HOVERGEN	HBG059833.
OMA	HIAPLES.
OrthoDB	EOG49GKHD.
PhylomeDB	Q969W9.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q969W9.
Bgee	Q969W9.
CleanEx	HS_PMEPA1. HS_STAG1.
Genevestigator	Q969W9.
GermOnline	ENSG00000124225. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	56937.
NextBio	62491.
SOURCE	Search...

Entry information

Entry name

PMEPA_HUMAN

Accession

Primary (citable) accession number: Q969W9
Secondary accession number(s): Q5TDR6 , Q8NER4, Q96B72, Q9UJD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.