ID   ZDH16_HUMAN             Reviewed;         377 AA.
AC   Q969W1; D3DR52; D3DR53; D3DR54; Q5JTG7; Q5JTH0; Q8N4Z6; Q8WY84; Q9BSV3;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   24-JAN-2024, entry version 167.
DE   RecName: Full=Palmitoyltransferase ZDHHC16 {ECO:0000305};
DE            EC=2.3.1.225 {ECO:0000269|PubMed:28826475};
DE   AltName: Full=Abl-philin 2 {ECO:0000303|PubMed:17123647};
DE   AltName: Full=Zinc finger DHHC domain-containing protein 16;
DE            Short=DHHC-16;
GN   Name=ZDHHC16 {ECO:0000312|HGNC:HGNC:20714};
GN   Synonyms=APH2 {ECO:0000303|PubMed:17123647}; ORFNames=UNQ2570/PRO6258;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, ALTERNATIVE
RP   SPLICING, TISSUE SPECIFICITY, AND INTERACTION WITH COPS5.
RC   TISSUE=Placenta;
RX   PubMed=17123647; DOI=10.1016/j.bbaexp.2006.10.002;
RA   Zhang F., Di Y., Li J., Shi Y., Zhang L., Wang C., He X., Liu Y., Wan D.,
RA   Huo K., Gu J.;
RT   "Molecular cloning and characterization of human Aph2 gene, involved in AP-
RT   1 regulation by interaction with JAB1.";
RL   Biochim. Biophys. Acta 1759:514-525(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA   Sarafidou T., Moschonas N.K.;
RT   "A novel gene containing the cysteine-rich zf-DHHC motif is localised at
RT   human chromosomal region 10q23.3-q24.";
RL   Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164054; DOI=10.1038/nature02462;
RA   Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA   Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA   Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA   Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA   Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA   Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA   Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA   Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA   Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA   Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA   Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA   Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA   McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA   Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA   Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA   Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA   Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA   Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA   Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA   Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA   Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 10.";
RL   Nature 429:375-381(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
RC   TISSUE=B-cell, Brain, Ovary, and Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=28826475; DOI=10.7554/elife.27826;
RA   Abrami L., Dallavilla T., Sandoz P.A., Demir M., Kunz B., Savoglidis G.,
RA   Hatzimanikatis V., van der Goot F.G.;
RT   "Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation
RT   cascade.";
RL   Elife 6:0-0(2017).
CC   -!- FUNCTION: Palmitoyl acyltransferase that mediates palmitoylation of
CC       proteins such as PLN and ZDHHC6 (PubMed:28826475). Required during
CC       embryonic heart development and cardiac function, possibly by mediating
CC       palmitoylation of PLN, thereby affecting PLN phosphorylation and
CC       homooligomerization (By similarity). Also required for eye development
CC       (By similarity). Palmitoylates ZDHHC6, affecting the quaternary
CC       assembly of ZDHHC6, its localization, stability and function
CC       (PubMed:28826475). May play a role in DNA damage response (By
CC       similarity). May be involved in apoptosis regulation (By similarity).
CC       Involved in the proliferation of neural stem cells by regulating the
CC       FGF/ERK pathway (By similarity). {ECO:0000250|UniProtKB:B8A4F0,
CC       ECO:0000250|UniProtKB:Q9ESG8, ECO:0000269|PubMed:28826475}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC         hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC         COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC         EC=2.3.1.225; Evidence={ECO:0000269|PubMed:28826475};
CC   -!- SUBUNIT: Interacts with ABL1 (By similarity). Interacts with COPS5/JAB1
CC       (PubMed:17123647). {ECO:0000250|UniProtKB:Q9ESG8,
CC       ECO:0000269|PubMed:17123647}.
CC   -!- INTERACTION:
CC       Q969W1; Q6PL24: TMED8; NbExp=3; IntAct=EBI-11572692, EBI-11603430;
CC       Q969W1; O95070: YIF1A; NbExp=3; IntAct=EBI-11572692, EBI-2799703;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000305|PubMed:17123647}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q9ESG8}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=Q969W1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q969W1-2; Sequence=VSP_008716;
CC       Name=3;
CC         IsoId=Q969W1-3; Sequence=VSP_016275;
CC       Name=4;
CC         IsoId=Q969W1-4; Sequence=VSP_016274, VSP_008716;
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:17123647}.
CC   -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC       {ECO:0000250|UniProtKB:Q8IUH5}.
CC   -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AF258563; AAG23766.1; -; mRNA.
DR   EMBL; AJ294945; CAC82556.1; -; mRNA.
DR   EMBL; AY359080; AAQ89439.1; -; mRNA.
DR   EMBL; AL355490; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471066; EAW49929.1; -; Genomic_DNA.
DR   EMBL; CH471066; EAW49930.1; -; Genomic_DNA.
DR   EMBL; CH471066; EAW49931.1; -; Genomic_DNA.
DR   EMBL; CH471066; EAW49932.1; -; Genomic_DNA.
DR   EMBL; CH471066; EAW49933.1; -; Genomic_DNA.
DR   EMBL; CH471066; EAW49934.1; -; Genomic_DNA.
DR   EMBL; BC004535; AAH04535.1; -; mRNA.
DR   EMBL; BC008074; AAH08074.1; -; mRNA.
DR   EMBL; BC011749; AAH11749.1; -; mRNA.
DR   EMBL; BC012830; AAH12830.1; -; mRNA.
DR   EMBL; BC033157; AAH33157.1; -; mRNA.
DR   CCDS; CCDS7460.1; -. [Q969W1-1]
DR   CCDS; CCDS7461.1; -. [Q969W1-2]
DR   CCDS; CCDS7462.1; -. [Q969W1-3]
DR   CCDS; CCDS7463.1; -. [Q969W1-4]
DR   RefSeq; NP_001274732.1; NM_001287803.1.
DR   RefSeq; NP_001274733.1; NM_001287804.1.
DR   RefSeq; NP_115703.2; NM_032327.3. [Q969W1-1]
DR   RefSeq; NP_932160.1; NM_198043.2. [Q969W1-2]
DR   RefSeq; NP_932161.1; NM_198044.2. [Q969W1-3]
DR   RefSeq; NP_932162.1; NM_198045.2. [Q969W1-4]
DR   RefSeq; NP_932163.1; NM_198046.2. [Q969W1-1]
DR   RefSeq; XP_016872255.1; XM_017016766.1. [Q969W1-2]
DR   RefSeq; XP_016872257.1; XM_017016768.1. [Q969W1-3]
DR   AlphaFoldDB; Q969W1; -.
DR   SMR; Q969W1; -.
DR   BioGRID; 124014; 9.
DR   IntAct; Q969W1; 3.
DR   STRING; 9606.ENSP00000377357; -.
DR   GlyGen; Q969W1; 1 site, 2 O-linked glycans (1 site).
DR   iPTMnet; Q969W1; -.
DR   PhosphoSitePlus; Q969W1; -.
DR   SwissPalm; Q969W1; -.
DR   BioMuta; ZDHHC16; -.
DR   DMDM; 37999852; -.
DR   MassIVE; Q969W1; -.
DR   MaxQB; Q969W1; -.
DR   PaxDb; 9606-ENSP00000377357; -.
DR   PeptideAtlas; Q969W1; -.
DR   ProteomicsDB; 75856; -. [Q969W1-1]
DR   ProteomicsDB; 75857; -. [Q969W1-2]
DR   ProteomicsDB; 75858; -. [Q969W1-3]
DR   ProteomicsDB; 75859; -. [Q969W1-4]
DR   Antibodypedia; 30898; 154 antibodies from 20 providers.
DR   DNASU; 84287; -.
DR   Ensembl; ENST00000345745.9; ENSP00000304487.8; ENSG00000171307.19. [Q969W1-4]
DR   Ensembl; ENST00000352634.8; ENSP00000345383.4; ENSG00000171307.19. [Q969W1-2]
DR   Ensembl; ENST00000353979.7; ENSP00000323360.3; ENSG00000171307.19. [Q969W1-3]
DR   Ensembl; ENST00000370842.6; ENSP00000359879.1; ENSG00000171307.19. [Q969W1-2]
DR   Ensembl; ENST00000370854.7; ENSP00000359891.3; ENSG00000171307.19. [Q969W1-1]
DR   Ensembl; ENST00000393760.6; ENSP00000377357.1; ENSG00000171307.19. [Q969W1-1]
DR   GeneID; 84287; -.
DR   KEGG; hsa:84287; -.
DR   MANE-Select; ENST00000393760.6; ENSP00000377357.1; NM_198046.3; NP_932163.1.
DR   UCSC; uc001knj.5; human. [Q969W1-1]
DR   AGR; HGNC:20714; -.
DR   CTD; 84287; -.
DR   DisGeNET; 84287; -.
DR   GeneCards; ZDHHC16; -.
DR   HGNC; HGNC:20714; ZDHHC16.
DR   HPA; ENSG00000171307; Low tissue specificity.
DR   MIM; 616750; gene.
DR   neXtProt; NX_Q969W1; -.
DR   OpenTargets; ENSG00000171307; -.
DR   PharmGKB; PA134985701; -.
DR   VEuPathDB; HostDB:ENSG00000171307; -.
DR   eggNOG; KOG1313; Eukaryota.
DR   GeneTree; ENSGT00940000155032; -.
DR   HOGENOM; CLU_054274_0_1_1; -.
DR   InParanoid; Q969W1; -.
DR   OMA; DGIVWDC; -.
DR   OrthoDB; 6683at2759; -.
DR   PhylomeDB; Q969W1; -.
DR   TreeFam; TF320809; -.
DR   BRENDA; 2.3.1.225; 2681.
DR   PathwayCommons; Q969W1; -.
DR   SignaLink; Q969W1; -.
DR   BioGRID-ORCS; 84287; 16 hits in 1158 CRISPR screens.
DR   ChiTaRS; ZDHHC16; human.
DR   GenomeRNAi; 84287; -.
DR   Pharos; Q969W1; Tbio.
DR   PRO; PR:Q969W1; -.
DR   Proteomes; UP000005640; Chromosome 10.
DR   RNAct; Q969W1; Protein.
DR   Bgee; ENSG00000171307; Expressed in pancreatic ductal cell and 179 other cell types or tissues.
DR   ExpressionAtlas; Q969W1; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016409; F:palmitoyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0006974; P:DNA damage response; ISS:UniProtKB.
DR   GO; GO:0001654; P:eye development; ISS:UniProtKB.
DR   GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR   GO; GO:0018345; P:protein palmitoylation; IDA:UniProtKB.
DR   GO; GO:0021537; P:telencephalon development; ISS:UniProtKB.
DR   InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR   PANTHER; PTHR12246; PALMITOYLTRANSFERASE ZDHHC16; 1.
DR   PANTHER; PTHR12246:SF13; PALMITOYLTRANSFERASE ZDHHC16; 1.
DR   Pfam; PF01529; DHHC; 1.
DR   PROSITE; PS50216; DHHC; 1.
DR   Genevisible; Q969W1; HS.
PE   1: Evidence at protein level;
KW   Acyltransferase; Alternative splicing; Apoptosis; DNA damage;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..377
FT                   /note="Palmitoyltransferase ZDHHC16"
FT                   /id="PRO_0000212897"
FT   TOPO_DOM        1..77
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        99..116
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        138..198
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        220..266
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        288..377
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          155..205
FT                   /note="DHHC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT   ACT_SITE        185
FT                   /note="S-palmitoyl cysteine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q8IUH5"
FT   VAR_SEQ         82..146
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_016274"
FT   VAR_SEQ         176..214
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_016275"
FT   VAR_SEQ         231..246
FT                   /note="Missing (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:12975309,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_008716"
SQ   SEQUENCE   377 AA;  43633 MW;  68AC733DE498A81E CRC64;
     MRGQRSLLLG PARLCLRLLL LLGYRRRCPP LLRGLVQRWR YGKVCLRSLL YNSFGGSDTA
     VDAAFEPVYW LVDNVIRWFG VVFVVLVIVL TGSIVAIAYL CVLPLILRTY SVPRLCWHFF
     YSHWNLILIV FHYYQAITTP PGYPPQGRND IATVSICKKC IYPKPARTHH CSICNRCVLK
     MDHHCPWLNN CVGHYNHRYF FSFCFFMTLG CVYCSYGSWD LFREAYAAIE KMKQLDKNKL
     QAVANQTYHQ TPPPTFSFRE RMTHKSLVYL WFLCSSVALA LGALTVWHAV LISRGETSIE
     RHINKKERRR LQAKGRVFRN PYNYGCLDNW KVFLGVDTGR HWLTRVLLPS SHLPHGNGMS
     WEPPPWVTAH SASVMAV
//