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Protein

Proteasome assembly chaperone 2

Gene

PSMG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome assembly chaperone 2
Short name:
PAC-2
Alternative name(s):
Hepatocellular carcinoma-susceptibility protein 3
Tumor necrosis factor superfamily member 5-induced protein 1
Gene namesi
Name:PSMG2Imported
Synonyms:HCCA3Imported, PAC2Imported, TNFSF5IP1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:24929. PSMG2.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162400246.

Polymorphism and mutation databases

BioMutaiPSMG2.
DMDMi74731063.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Proteasome assembly chaperone 2PRO_0000322552Add
BLAST

Post-translational modificationi

Degraded by the proteasome upon completion of 20S proteasome maturation.1 Publication

Proteomic databases

EPDiQ969U7.
MaxQBiQ969U7.
PaxDbiQ969U7.
PRIDEiQ969U7.

PTM databases

iPTMnetiQ969U7.
PhosphoSiteiQ969U7.

Expressioni

Tissue specificityi

Widely expressed with highest levels in lung, brain and colon. Moderately expressed in muscle, stomach, spleen and heart. Weakly expressed in small intestine, pancreas and liver. Highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue.1 Publication

Gene expression databases

BgeeiQ969U7.
CleanExiHS_PSMG2.
ExpressionAtlasiQ969U7. baseline and differential.
GenevisibleiQ969U7. HS.

Organism-specific databases

HPAiHPA041517.
HPA046741.

Interactioni

Subunit structurei

Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153233EBI-723276,EBI-948001
NOTCH2NLQ7Z3S93EBI-723276,EBI-945833
TCF4P158843EBI-723276,EBI-533224

Protein-protein interaction databases

BioGridi121301. 48 interactions.
IntActiQ969U7. 10 interactions.
MINTiMINT-6176023.
STRINGi9606.ENSP00000325919.

Structurei

3D structure databases

ProteinModelPortaliQ969U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PSMG2 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3112. Eukaryota.
ENOG410XRHJ. LUCA.
GeneTreeiENSGT00390000018415.
HOGENOMiHOG000046033.
HOVERGENiHBG059464.
InParanoidiQ969U7.
KOiK11876.
OMAiMTINDYY.
OrthoDBiEOG7BKCW2.
PhylomeDBiQ969U7.
TreeFamiTF105397.

Family and domain databases

InterProiIPR019151. Proteasome_assmbl_chaperone_2.
IPR016562. Proteasome_assmbl_chp_2_euk.
[Graphical view]
PfamiPF09754. PAC2. 1 hit.
[Graphical view]
PIRSFiPIRSF010044. UCP010044. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969U7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFVPCGESAP DLAGFTLLMP AVSVGNVGQL AMDLIISTLN MSKIGYFYTD
60 70 80 90 100
CLVPMVGNNP YATTEGNSTE LSINAEVYSL PSRKLVALQL RSIFIKYKSK
110 120 130 140 150
PFCEKLLSWV KSSGCARVIV LSSSHSYQRN DLQLRSTPFR YLLTPSMQKS
160 170 180 190 200
VQNKIKSLNW EEMEKSRCIP EIDDSEFCIR IPGGGITKTL YDESCSKEIQ
210 220 230 240 250
MAVLLKFVSE GDNIPDALGL VEYLNEWLQI LKPLSDDPTV SASRWKIPSS
260
WRLLFGSGLP PALF
Length:264
Mass (Da):29,396
Last modified:December 1, 2001 - v1
Checksum:i7D138B81C3C20EF9
GO
Isoform 2 (identifier: Q969U7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Show »
Length:233
Mass (Da):26,321
Checksum:i8910F04AC6B83BEB
GO

Sequence cautioni

The sequence AAF87314.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171R → E in AAF87314 (PubMed:11920191).Curated
Sequence conflicti164 – 1641E → D in AAF87314 (PubMed:11920191).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3131Missing in isoform 2. 1 PublicationVSP_055721Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168712 mRNA. Translation: AAF87314.1. Different initiation.
AF276707 mRNA. Translation: AAK69439.2.
EF445040 Genomic DNA. Translation: ACA06090.1.
AK057005 mRNA. Translation: BAG51841.1.
AP005482 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01540.1.
CH471113 Genomic DNA. Translation: EAX01541.1.
BC013356 mRNA. Translation: AAH13356.1.
CR457181 mRNA. Translation: CAG33462.1.
BR000237 mRNA. Translation: FAA00023.1.
CCDSiCCDS11862.1. [Q969U7-1]
CCDS67440.1. [Q969U7-2]
RefSeqiNP_064617.2. NM_020232.4. [Q969U7-1]
NP_671692.1. NM_147163.1. [Q969U7-2]
UniGeneiHs.464652.

Genome annotation databases

EnsembliENST00000317615; ENSP00000325919; ENSG00000128789. [Q969U7-1]
ENST00000585331; ENSP00000476763; ENSG00000128789. [Q969U7-2]
GeneIDi56984.
KEGGihsa:56984.
UCSCiuc002krg.5. human. [Q969U7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168712 mRNA. Translation: AAF87314.1. Different initiation.
AF276707 mRNA. Translation: AAK69439.2.
EF445040 Genomic DNA. Translation: ACA06090.1.
AK057005 mRNA. Translation: BAG51841.1.
AP005482 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01540.1.
CH471113 Genomic DNA. Translation: EAX01541.1.
BC013356 mRNA. Translation: AAH13356.1.
CR457181 mRNA. Translation: CAG33462.1.
BR000237 mRNA. Translation: FAA00023.1.
CCDSiCCDS11862.1. [Q969U7-1]
CCDS67440.1. [Q969U7-2]
RefSeqiNP_064617.2. NM_020232.4. [Q969U7-1]
NP_671692.1. NM_147163.1. [Q969U7-2]
UniGeneiHs.464652.

3D structure databases

ProteinModelPortaliQ969U7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121301. 48 interactions.
IntActiQ969U7. 10 interactions.
MINTiMINT-6176023.
STRINGi9606.ENSP00000325919.

PTM databases

iPTMnetiQ969U7.
PhosphoSiteiQ969U7.

Polymorphism and mutation databases

BioMutaiPSMG2.
DMDMi74731063.

Proteomic databases

EPDiQ969U7.
MaxQBiQ969U7.
PaxDbiQ969U7.
PRIDEiQ969U7.

Protocols and materials databases

DNASUi56984.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317615; ENSP00000325919; ENSG00000128789. [Q969U7-1]
ENST00000585331; ENSP00000476763; ENSG00000128789. [Q969U7-2]
GeneIDi56984.
KEGGihsa:56984.
UCSCiuc002krg.5. human. [Q969U7-1]

Organism-specific databases

CTDi56984.
GeneCardsiPSMG2.
HGNCiHGNC:24929. PSMG2.
HPAiHPA041517.
HPA046741.
MIMi609702. gene.
neXtProtiNX_Q969U7.
PharmGKBiPA162400246.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3112. Eukaryota.
ENOG410XRHJ. LUCA.
GeneTreeiENSGT00390000018415.
HOGENOMiHOG000046033.
HOVERGENiHBG059464.
InParanoidiQ969U7.
KOiK11876.
OMAiMTINDYY.
OrthoDBiEOG7BKCW2.
PhylomeDBiQ969U7.
TreeFamiTF105397.

Miscellaneous databases

ChiTaRSiPSMG2. human.
GenomeRNAii56984.
NextBioi62663.
PROiQ969U7.
SOURCEiSearch...

Gene expression databases

BgeeiQ969U7.
CleanExiHS_PSMG2.
ExpressionAtlasiQ969U7. baseline and differential.
GenevisibleiQ969U7. HS.

Family and domain databases

InterProiIPR019151. Proteasome_assmbl_chaperone_2.
IPR016562. Proteasome_assmbl_chp_2_euk.
[Graphical view]
PfamiPF09754. PAC2. 1 hit.
[Graphical view]
PIRSFiPIRSF010044. UCP010044. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene expression in CD34(+) cells from normal bone marrow and leukemic origins."
    Gu J., Zhang Q.H., Huang Q.H., Ren S.X., Wu X.Y., Ye M., Huang C.H., Fu G., Zhou J., Niu C., Han Z.G., Chen S.J., Chen Z.
    Hematol. J. 1:206-217(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Hematopoietic stem cell.
  2. "Expression of liver cancer associated gene HCCA3."
    Wang Z.-X., Hu G.-F., Wang H.-Y., Wu M.-C.
    World J. Gastroenterol. 7:821-825(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Placenta1 Publication.
  3. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: PancreasImported.
  8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-264 (ISOFORM 1).
  9. "A heterodimeric complex that promotes the assembly of mammalian 20S proteasomes."
    Hirano Y., Hendil K.B., Yashiroda H., Iemura S., Nagane R., Hioki Y., Natsume T., Tanaka K., Murata S.
    Nature 437:1381-1385(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION, INTERACTION WITH PSMA5; PSMA7 AND PSMG1, DEGRADATION BY THE PROTEASOME.
  10. "20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals."
    Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R., Marsolier-Kergoat M.-C., Peyroche A.
    Mol. Cell 27:660-674(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSMG2_HUMAN
AccessioniPrimary (citable) accession number: Q969U7
Secondary accession number(s): B0YJB3
, Q6IAH4, Q9NRV1, V9GYH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 1, 2001
Last modified: April 13, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.