Reviewed, UniProtKB/Swiss-Prot Q969U7 (PSMG2_HUMAN)

Names and origin

Protein names

Recommended name:
    Proteasome assembly chaperone 2
      Short name=PAC-2
Alternative name(s):
    Tumor necrosis factor superfamily member 5-induced protein 1
    Hepatocellular carcinoma-susceptibility protein 3

Gene names

Name:	PSMG2
Synonyms:	HCCA3, PAC2, TNFSF5IP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization. Ref.5 Ref.6
Subunit structure	Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits. Ref.5
Subcellular location	Nucleus By similarity. UniProtKB Q9EST4
Tissue specificity	Widely expressed with highest levels in lung, brain and colon. Moderately expressed in muscle, stomach, spleen and heart. Weakly expressed in small intestine, pancreas and liver. Highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. Ref.1
Post-translational modification	Degraded by the proteasome upon completion of 20S proteasome maturation. Ref.5
Sequence similarities	Belongs to the PSMG2 family.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Chaperone
Gene Ontology (GO)
Biological process	proteasome assembly Ref.5 Inferred from direct assay. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Proteasome assembly chaperone 2		PRO_0000322552

Sequences

	Sequence		Length	Mass (Da)
	Q969U7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7D138B81C3C20EF9	FASTA	264	29,396
	10 20 30 40 50 60 MFVPCGESAP DLAGFTLLMP AVSVGNVGQL AMDLIISTLN MSKIGYFYTD CLVPMVGNNP 70 80 90 100 110 120 YATTEGNSTE LSINAEVYSL PSRKLVALQL RSIFIKYKSK PFCEKLLSWV KSSGCARVIV 130 140 150 160 170 180 LSSSHSYQRN DLQLRSTPFR YLLTPSMQKS VQNKIKSLNW EEMEKSRCIP EIDDSEFCIR 190 200 210 220 230 240 IPGGGITKTL YDESCSKEIQ MAVLLKFVSE GDNIPDALGL VEYLNEWLQI LKPLSDDPTV 250 260 SASRWKIPSS WRLLFGSGLP PALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of liver cancer associated gene HCCA3." Wang Z.-X., Hu G.-F., Wang H.-Y., Wu M.-C. World J. Gastroenterol. 7:821-825(2001) [PubMed: 11854909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-264.
[5]	"A heterodimeric complex that promotes the assembly of mammalian 20S proteasomes." Hirano Y., Hendil K.B., Yashiroda H., Iemura S., Nagane R., Hioki Y., Natsume T., Tanaka K., Murata S. Nature 437:1381-1385(2005) [PubMed: 16251969] [Abstract] Cited for: IDENTIFICATION, FUNCTION, INTERACTION WITH PSMA5; PSMA7 AND PSMG1, DEGRADATION BY THE PROTEASOME.
[6]	"20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals." Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R., Marsolier-Kergoat M.-C., Peyroche A. Mol. Cell 27:660-674(2007) [PubMed: 17707236] [Abstract] Cited for: FUNCTION.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF276707 mRNA. Translation: AAK69439.2. CH471113 Genomic DNA. Translation: EAX01540.1. BC013356 mRNA. Translation: AAH13356.1. CR457181 mRNA. Translation: CAG33462.1. BR000237 mRNA. Translation: FAA00023.1.
IPI	IPI00644482.
RefSeq	NP_064617.2.
UniGene	Hs.464652
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969U7. 1 interaction.
Proteomic databases
PRIDE	Q969U7.
Genome annotation databases
Ensembl	ENSG00000128789. Homo sapiens. [Contig view]
GeneID	56984.
KEGG	hsa:56984.
Organism-specific databases
GeneCards	GC18P012684.
HGNC	HGNC:24929. PSMG2.
MIM	609702. gene.
PharmGKB	PA134982165.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q969U7.
HOVERGEN	Q969U7.
OMA	Q969U7. RSIFIKY.
Gene expression databases
ArrayExpress	Q969U7.
Bgee	Q969U7.
CleanEx	HS_PSMG2.
Family and domain databases
InterPro	IPR019151. 20S_Psome_assmbly_chp2. IPR016562. 20S_Psome_chp2_sbgrp. [Graphical view]
Pfam	PF09754. HCCA3. 1 hit. [Graphical view]
PIRSF	PIRSF010044. UCP010044. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	62663.
SOURCE	Search...

Entry information

Entry name

PSMG2_HUMAN

Accession

Primary (citable) accession number: Q969U7
Secondary accession number(s): Q6IAH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)