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Q969T7

- 5NT3B_HUMAN

UniProt

Q969T7 - 5NT3B_HUMAN

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Protein
7-methylguanosine phosphate-specific 5'-nucleotidase
Gene
NT5C3B, NT5C3L
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.1 Publication

Catalytic activityi

N(7)-methyl-GMP + H2O = N(7)-methyl-guanosine + phosphate.1 Publication
CMP + H2O = cytidine + phosphate.1 Publication
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.1 Publication

Kineticsi

kcat is 0.24 sec(-1) with m7GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP.

  1. KM=7.8 µM for m7GMP1 Publication
  2. KM=79 µM for CMP
  3. KM=355 µM for GMP
  4. KM=456 µM for AMP
  5. KM=439 µM for UMP

Vmax=0.41 µmol/min/mg enzyme with m7GMP as substrate

Vmax=12 µmol/min/mg enzyme with CMP as substrate

Vmax=0.13 µmol/min/mg enzyme with GMP as substrate

Vmax=0.07 µmol/min/mg enzyme with AMP as substrate

Vmax=10.7 µmol/min/mg enzyme with UMP as substrate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411Nucleophile By similarity
Metal bindingi41 – 411Magnesium By similarity
Active sitei43 – 431Proton donor By similarity
Metal bindingi43 – 431Magnesium; via carbonyl oxygen By similarity
Binding sitei205 – 2051Substrate By similarity
Metal bindingi230 – 2301Magnesium By similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. nucleotide metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ969T7.

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidase (EC:3.1.3.91)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like protein (EC:3.1.3.5)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:NT5C3B
Synonyms:NT5C3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:28300. NT5C3B.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3003007-methylguanosine phosphate-specific 5'-nucleotidase
PRO_0000328948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ969T7.
PaxDbiQ969T7.
PRIDEiQ969T7.

PTM databases

PhosphoSiteiQ969T7.

Expressioni

Gene expression databases

ArrayExpressiQ969T7.
BgeeiQ969T7.
CleanExiHS_NT5C3L.
GenevestigatoriQ969T7.

Organism-specific databases

HPAiHPA030786.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P406923EBI-2932564,EBI-744248

Protein-protein interaction databases

IntActiQ969T7. 1 interaction.
STRINGi9606.ENSP00000269534.

Structurei

3D structure databases

ProteinModelPortaliQ969T7.
SMRiQ969T7. Positions 18-289.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 1572Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG266578.
HOVERGENiHBG059750.
InParanoidiQ969T7.
KOiK01081.
OMAiNDKVEEQ.
TreeFamiTF314663.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PANTHERiPTHR13045. PTHR13045. 1 hit.
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q969T7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA    50
YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP 100
HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI 150
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI 200
HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI 250
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP 300
Length:300
Mass (Da):34,389
Last modified:April 3, 2013 - v4
Checksum:i3E221583153381F8
GO
Isoform 2 (identifier: Q969T7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:292
Mass (Da):33,528
Checksum:i8ACC59D5F25E629A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti209 – 2091A → V.2 Publications
Corresponds to variant rs1046403 [ dbSNP | Ensembl ].
VAR_042582
Natural varianti213 – 2131S → C.2 Publications
Corresponds to variant rs1046404 [ dbSNP | Ensembl ].
VAR_042583

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88Missing in isoform 2.
VSP_046297

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC091172 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60771.1.
BC013742 mRNA. Translation: AAH13742.2.
BC014132 mRNA. Translation: AAH14132.2.
BC016971 mRNA. Translation: AAH16971.2.
BC067788 mRNA. Translation: AAH67788.1.
CCDSiCCDS11410.2. [Q969T7-1]
RefSeqiNP_443167.4. NM_052935.4. [Q969T7-1]
UniGeneiHs.237536.

Genome annotation databases

EnsembliENST00000269534; ENSP00000269534; ENSG00000141698. [Q969T7-2]
ENST00000435506; ENSP00000389948; ENSG00000141698. [Q969T7-1]
ENST00000569139; ENSP00000454620; ENSG00000260753. [Q969T7-1]
GeneIDi115024.
KEGGihsa:115024.
UCSCiuc002hxy.4. human. [Q969T7-1]

Polymorphism databases

DMDMi476007845.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC091172 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60771.1 .
BC013742 mRNA. Translation: AAH13742.2 .
BC014132 mRNA. Translation: AAH14132.2 .
BC016971 mRNA. Translation: AAH16971.2 .
BC067788 mRNA. Translation: AAH67788.1 .
CCDSi CCDS11410.2. [Q969T7-1 ]
RefSeqi NP_443167.4. NM_052935.4. [Q969T7-1 ]
UniGenei Hs.237536.

3D structure databases

ProteinModelPortali Q969T7.
SMRi Q969T7. Positions 18-289.
ModBasei Search...

Protein-protein interaction databases

IntActi Q969T7. 1 interaction.
STRINGi 9606.ENSP00000269534.

PTM databases

PhosphoSitei Q969T7.

Polymorphism databases

DMDMi 476007845.

Proteomic databases

MaxQBi Q969T7.
PaxDbi Q969T7.
PRIDEi Q969T7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000269534 ; ENSP00000269534 ; ENSG00000141698 . [Q969T7-2 ]
ENST00000435506 ; ENSP00000389948 ; ENSG00000141698 . [Q969T7-1 ]
ENST00000569139 ; ENSP00000454620 ; ENSG00000260753 . [Q969T7-1 ]
GeneIDi 115024.
KEGGi hsa:115024.
UCSCi uc002hxy.4. human. [Q969T7-1 ]

Organism-specific databases

CTDi 115024.
GeneCardsi GC17M039982.
HGNCi HGNC:28300. NT5C3B.
HPAi HPA030786.
neXtProti NX_Q969T7.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG266578.
HOVERGENi HBG059750.
InParanoidi Q969T7.
KOi K01081.
OMAi NDKVEEQ.
TreeFami TF314663.

Enzyme and pathway databases

SABIO-RK Q969T7.

Miscellaneous databases

GenomeRNAii 115024.
NextBioi 13644547.
PROi Q969T7.

Gene expression databases

ArrayExpressi Q969T7.
Bgeei Q969T7.
CleanExi HS_NT5C3L.
Genevestigatori Q969T7.

Family and domain databases

Gene3Di 3.40.50.1000. 2 hits.
InterProi IPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view ]
PANTHERi PTHR13045. PTHR13045. 1 hit.
Pfami PF05822. UMPH-1. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01544. HAD-SF-IE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS VAL-209 AND CYS-213.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS VAL-209 AND CYS-213.
    Tissue: Liver, Lung and Testis.
  4. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-256, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Identification of Drosophila and Human 7-Methyl GMP-specific Nucleotidases."
    Buschmann J., Moritz B., Jeske M., Lilie H., Schierhorn A., Wahle E.
    J. Biol. Chem. 288:2441-2451(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry namei5NT3B_HUMAN
AccessioniPrimary (citable) accession number: Q969T7
Secondary accession number(s): A8MWB9, C9JKC4, Q7L3B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: July 9, 2014
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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