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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

NT5C3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).2 Publications

Catalytic activityi

N(7)-methyl-GMP + H2O = N(7)-methyl-guanosine + phosphate.1 Publication
CMP + H2O = cytidine + phosphate.1 Publication
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.1 Publication

Kineticsi

kcat is 0.24 sec(-1) with m7GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP.

  1. KM=7.8 µM for m7GMP (at 37 degrees Celsius)1 Publication
  2. KM=8 µM for m7GMP (at 37 degrees Celsius)1 Publication
  3. KM=79 µM for CMP (at 37 degrees Celsius)2 Publications
  4. KM=355 µM for GMP (at 37 degrees Celsius)1 Publication
  5. KM=456 µM for AMP (at 37 degrees Celsius)1 Publication
  6. KM=439 µM for UMP (at 37 degrees Celsius)1 Publication
  1. Vmax=0.41 µmol/min/mg enzyme with m7GMP as substrate1 Publication
  2. Vmax=12 µmol/min/mg enzyme with CMP as substrate1 Publication
  3. Vmax=0.13 µmol/min/mg enzyme with GMP as substrate1 Publication
  4. Vmax=0.07 µmol/min/mg enzyme with AMP as substrate1 Publication
  5. Vmax=10.7 µmol/min/mg enzyme with UMP as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41NucleophileBy similarity1
Metal bindingi41MagnesiumBy similarity1
Active sitei43Proton donorBy similarity1
Metal bindingi43Magnesium; via carbonyl oxygenBy similarity1
Binding sitei88CMPBy similarity1
Binding sitei88N(7)-methyl-GMPBy similarity1
Binding sitei205SubstrateBy similarity1
Metal bindingi230MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS13906-MONOMER.
ReactomeiR-HSA-429958. mRNA decay by 3' to 5' exoribonuclease.
SABIO-RKQ969T7.

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidase1 Publication (EC:3.1.3.911 Publication)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like protein1 Publication (EC:3.1.3.51 Publication)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:NT5C3B
Synonyms:NT5C3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:28300. NT5C3B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000141698.

Polymorphism and mutation databases

BioMutaiNT5C3B.
DMDMi476007845.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289481 – 3007-methylguanosine phosphate-specific 5'-nucleotidaseAdd BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ969T7.
MaxQBiQ969T7.
PaxDbiQ969T7.
PeptideAtlasiQ969T7.
PRIDEiQ969T7.

PTM databases

DEPODiQ969T7.
iPTMnetiQ969T7.
PhosphoSitePlusiQ969T7.

Expressioni

Gene expression databases

BgeeiENSG00000141698.
CleanExiHS_NT5C3L.
ExpressionAtlasiQ969T7. baseline and differential.

Organism-specific databases

HPAiHPA030786.
HPA057095.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P406923EBI-2932564,EBI-744248

Protein-protein interaction databases

BioGridi125410. 4 interactors.
IntActiQ969T7. 1 interactor.
STRINGi9606.ENSP00000389948.

Structurei

3D structure databases

ProteinModelPortaliQ969T7.
SMRiQ969T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 157Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOGENOMiHOG000244931.
HOVERGENiHBG059750.
KOiK01081.
OMAiDPHRTVK.
OrthoDBiEOG091G0BCN.
TreeFamiTF314663.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969T7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA
60 70 80 90 100
YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP
110 120 130 140 150
HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI
160 170 180 190 200
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI
210 220 230 240 250
HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI
260 270 280 290 300
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP
Length:300
Mass (Da):34,389
Last modified:April 3, 2013 - v4
Checksum:i3E221583153381F8
GO
Isoform 2 (identifier: Q969T7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:292
Mass (Da):33,528
Checksum:i8ACC59D5F25E629A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042582209A → V.2 PublicationsCorresponds to variant rs1046403dbSNPEnsembl.1
Natural variantiVAR_042583213S → C.2 PublicationsCorresponds to variant rs1046404dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462971 – 8Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091172 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60771.1.
BC013742 mRNA. Translation: AAH13742.2.
BC014132 mRNA. Translation: AAH14132.2.
BC016971 mRNA. Translation: AAH16971.2.
BC067788 mRNA. Translation: AAH67788.1.
CCDSiCCDS11410.2. [Q969T7-1]
RefSeqiNP_443167.4. NM_052935.4. [Q969T7-1]
UniGeneiHs.237536.

Genome annotation databases

EnsembliENST00000435506; ENSP00000389948; ENSG00000141698. [Q969T7-1]
GeneIDi115024.
KEGGihsa:115024.
UCSCiuc021txo.2. human. [Q969T7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091172 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60771.1.
BC013742 mRNA. Translation: AAH13742.2.
BC014132 mRNA. Translation: AAH14132.2.
BC016971 mRNA. Translation: AAH16971.2.
BC067788 mRNA. Translation: AAH67788.1.
CCDSiCCDS11410.2. [Q969T7-1]
RefSeqiNP_443167.4. NM_052935.4. [Q969T7-1]
UniGeneiHs.237536.

3D structure databases

ProteinModelPortaliQ969T7.
SMRiQ969T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125410. 4 interactors.
IntActiQ969T7. 1 interactor.
STRINGi9606.ENSP00000389948.

PTM databases

DEPODiQ969T7.
iPTMnetiQ969T7.
PhosphoSitePlusiQ969T7.

Polymorphism and mutation databases

BioMutaiNT5C3B.
DMDMi476007845.

Proteomic databases

EPDiQ969T7.
MaxQBiQ969T7.
PaxDbiQ969T7.
PeptideAtlasiQ969T7.
PRIDEiQ969T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000435506; ENSP00000389948; ENSG00000141698. [Q969T7-1]
GeneIDi115024.
KEGGihsa:115024.
UCSCiuc021txo.2. human. [Q969T7-1]

Organism-specific databases

CTDi115024.
GeneCardsiNT5C3B.
HGNCiHGNC:28300. NT5C3B.
HPAiHPA030786.
HPA057095.
neXtProtiNX_Q969T7.
OpenTargetsiENSG00000141698.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOGENOMiHOG000244931.
HOVERGENiHBG059750.
KOiK01081.
OMAiDPHRTVK.
OrthoDBiEOG091G0BCN.
TreeFamiTF314663.

Enzyme and pathway databases

BioCyciZFISH:HS13906-MONOMER.
ReactomeiR-HSA-429958. mRNA decay by 3' to 5' exoribonuclease.
SABIO-RKQ969T7.

Miscellaneous databases

GenomeRNAii115024.
PROiQ969T7.

Gene expression databases

BgeeiENSG00000141698.
CleanExiHS_NT5C3L.
ExpressionAtlasiQ969T7. baseline and differential.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei5NT3B_HUMAN
AccessioniPrimary (citable) accession number: Q969T7
Secondary accession number(s): A8MWB9, C9JKC4, Q7L3B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: November 2, 2016
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.