Q969T7 (5NT3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidase
EC=3.1.3.-

Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like protein
Short name=cN-III-like protein
EC=3.1.3.5

Gene names

Name:	NT5C3B
Synonyms:	NT5C3L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates. Ref.6
Catalytic activity	7-methylguanosine 5'-phosphate + H2O = a 7-methylguanosine + phosphate. Ref.6 A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. Ref.6
Subunit structure	Monomer. Ref.6
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the pyrimidine 5'-nucleotidase family.
Biophysicochemical properties	Kinetic parameters: kcat is 0.24 sec(-1) with m7GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP. KM=7.8 µM for m7GMP Ref.6 KM=79 µM for CMP KM=355 µM for GMP KM=456 µM for AMP KM=439 µM for UMP Vmax=0.41 µmol/min/mg enzyme with m7GMP as substrate Vmax=12 µmol/min/mg enzyme with CMP as substrate Vmax=0.13 µmol/min/mg enzyme with GMP as substrate Vmax=0.07 µmol/min/mg enzyme with AMP as substrate Vmax=10.7 µmol/min/mg enzyme with UMP as substrate

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MLH1	P40692	3	EBI-2932564,EBI-744248

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q969T7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q969T7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	7-methylguanosine phosphate-specific 5'-nucleotidase		PRO_0000328948
Regions
	Region	156 – 157	2	Substrate binding By similarity
Sites
	Active site	41	1	Nucleophile By similarity
	Active site	43	1	Proton donor By similarity
	Metal binding	41	1	Magnesium By similarity
	Metal binding	43	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	230	1	Magnesium By similarity
	Binding site	205	1	Substrate By similarity
Amino acid modifications
	Modified residue	256	1	N6-acetyllysine Ref.4
Natural variations
	Alternative sequence	1 – 8	8	Missing in isoform 2.		VSP_046297
	Natural variant	209	1	A → V. Ref.2 Ref.3 Corresponds to variant rs1046403 [ dbSNP | Ensembl ].		VAR_042582
	Natural variant	213	1	S → C. Ref.2 Ref.3 Corresponds to variant rs1046404 [ dbSNP | Ensembl ].		VAR_042583

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2013. Version 4. Checksum: 3E221583153381F8	FASTA	300	34,389
	10 20 30 40 50 60 MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA YNGKRCPSSY 70 80 90 100 110 120 NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP HMVEWWTKAH NLLCQQKIQK 130 140 150 160 170 180 FQIAQVVRES NAMLREGYKT FFNTLYHNNI PLFIFSAGIG DILEEIIRQM KVFHPNIHIV 190 200 210 220 230 240 SNYMDFNEDG FLQGFKGQLI HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG 250 260 270 280 290 300 VPGVQNILKI GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP
	Isoform 2 [UniParc]. Checksum: 8ACC59D5F25E629A	FASTA	292	33,528
	10 20 30 40 50 60 MKATVLMRQP GRVQEIVGAL RKGGGDRLQV ISDFDMTLSR FAYNGKRCPS SYNILDNSKI 70 80 90 100 110 120 ISEECRKELT ALLHHYYPIE IDPHRTVKEK LPHMVEWWTK AHNLLCQQKI QKFQIAQVVR 130 140 150 160 170 180 ESNAMLREGY KTFFNTLYHN NIPLFIFSAG IGDILEEIIR QMKVFHPNIH IVSNYMDFNE 190 200 210 220 230 240 DGFLQGFKGQ LIHTYNKNSS ACENSGYFQQ LEGKTNVILL GDSIGDLTMA DGVPGVQNIL 250 260 270 280 290 KIGFLNDKVE ERRERYMDSY DIVLEKDETL DVVNGLLQHI LCQGVQLEMQ GP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS VAL-209 AND CYS-213.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS VAL-209 AND CYS-213. Tissue: Liver, Lung and Testis.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-256, MASS SPECTROMETRY.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Identification of Drosophila and Human 7-Methyl GMP-specific Nucleotidases." Buschmann J., Moritz B., Jeske M., Lilie H., Schierhorn A., Wahle E. J. Biol. Chem. 288:2441-2451(2013) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC091172 Genomic DNA. No translation available. CH471152 Genomic DNA. Translation: EAW60771.1. BC013742 mRNA. Translation: AAH13742.2. BC014132 mRNA. Translation: AAH14132.2. BC016971 mRNA. Translation: AAH16971.2. BC067788 mRNA. Translation: AAH67788.1.
IPI	IPI00056505.
RefSeq	NP_443167.4. NM_052935.4.
UniGene	Hs.237536.
3D structure databases
HSSP	HSSP built from PDB template 2CN1 based on UniProtKB Q9UC45.
ProteinModelPortal	Q969T7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969T7. 1 interaction.
STRING	9606.ENSP00000269534.
PTM databases
PhosphoSite	Q969T7.
Polymorphism databases
DMDM	296439454.
Proteomic databases
PaxDb	Q969T7.
PRIDE	Q969T7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000269534; ENSP00000269534; ENSG00000141698. ENST00000435506; ENSP00000389948; ENSG00000141698. ENST00000569139; ENSP00000454620; ENSG00000260753.
GeneID	115024.
KEGG	hsa:115024.
UCSC	uc002hxy.4. human.
Organism-specific databases
CTD	115024.
GeneCards	GC17M039981.
HGNC	HGNC:28300. NT5C3B.
HPA	HPA030786.
neXtProt	NX_Q969T7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG266578.
HOVERGEN	HBG059750.
InParanoid	Q969T7.
KO	K01081.
OrthoDB	EOG4JWVDW.
PhylomeDB	Q969T7.
Gene expression databases
ArrayExpress	Q969T7.
Bgee	Q969T7.
CleanEx	HS_NT5C3L.
Genevestigator	Q969T7.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR023214. HAD-like_dom. IPR006434. Pyrimidine_nucleotidase_eu. [Graphical view]
PANTHER	PTHR13045. PTHR13045. 1 hit.
Pfam	PF05822. UMPH-1. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01544. HAD-SF-IE. 1 hit.
ProtoNet	Search...
Other
NextBio	13644547.

Entry information

Entry name

5NT3B_HUMAN

Accession

Primary (citable) accession number: Q969T7
Secondary accession number(s): A8MWB9, C9JKC4, Q7L3B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 3, 2013
Last modified:	May 1, 2013
This is version 95 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.