Q969T7 (5NT3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic 5'-nucleotidase III-like protein
Short name=cN-III-like protein
EC=3.1.3.5

Gene names

Name:

NT5C3L

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can act both as nucleotidase and as phosphotransferase By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the pyrimidine 5'-nucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MLH1	P40692	3	EBI-2932564,EBI-744248

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Cytosolic 5'-nucleotidase III-like protein		PRO_0000328948
Regions
	Region	148 – 149	2	Substrate binding By similarity
Sites
	Active site	33	1	Nucleophile By similarity
	Active site	35	1	Proton donor By similarity
	Metal binding	33	1	Magnesium By similarity
	Metal binding	35	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	222	1	Magnesium By similarity
	Binding site	197	1	Substrate By similarity
Amino acid modifications
	Modified residue	248	1	N6-acetyllysine Ref.4
Natural variations
	Natural variant	201	1	A → V. Ref.2 Ref.3 Corresponds to variant rs1046403 [ dbSNP | Ensembl ].		VAR_042582
	Natural variant	205	1	S → C. Ref.2 Ref.3 Corresponds to variant rs1046404 [ dbSNP | Ensembl ].		VAR_042583

Sequences

	Sequence		Length	Mass (Da)
	Q969T7 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 8ACC59D5F25E629A	FASTA	292	33,528
	10 20 30 40 50 60 MKATVLMRQP GRVQEIVGAL RKGGGDRLQV ISDFDMTLSR FAYNGKRCPS SYNILDNSKI 70 80 90 100 110 120 ISEECRKELT ALLHHYYPIE IDPHRTVKEK LPHMVEWWTK AHNLLCQQKI QKFQIAQVVR 130 140 150 160 170 180 ESNAMLREGY KTFFNTLYHN NIPLFIFSAG IGDILEEIIR QMKVFHPNIH IVSNYMDFNE 190 200 210 220 230 240 DGFLQGFKGQ LIHTYNKNSS ACENSGYFQQ LEGKTNVILL GDSIGDLTMA DGVPGVQNIL 250 260 270 280 290 KIGFLNDKVE ERRERYMDSY DIVLEKDETL DVVNGLLQHI LCQGVQLEMQ GP

References

[1]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS VAL-201 AND CYS-205.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS VAL-201 AND CYS-205. Tissue: Liver, Lung and Testis.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-248, MASS SPECTROMETRY.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC091172 Genomic DNA. No translation available. CH471152 Genomic DNA. Translation: EAW60771.1. BC013742 mRNA. Translation: AAH13742.2. BC014132 mRNA. Translation: AAH14132.2. BC016971 mRNA. Translation: AAH16971.2. BC067788 mRNA. Translation: AAH67788.1.
IPI	IPI00056505.
UniGene	Hs.237536.
3D structure databases
HSSP	HSSP built from PDB template 2CN1 based on UniProtKB Q9UC45.
ProteinModelPortal	Q969T7.
SMR	Q969T7. Positions 1-281.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969T7. 1 interaction.
STRING	Q969T7.
PTM databases
PhosphoSite	Q969T7.
Polymorphism databases
DMDM	296439454.
Proteomic databases
PRIDE	Q969T7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000269534; ENSP00000269534; ENSG00000141698. ENST00000393911; ENSP00000377488; ENSG00000141698.
UCSC	uc002hxx.2. human.
Organism-specific databases
GeneCards	GC17M039981.
HGNC	HGNC:28300. NT5C3L.
HPA	HPA030786.
neXtProt	NX_Q969T7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06021.
GeneTree	ENSGT00390000012959.
HOGENOM	HBG382723.
HOVERGEN	HBG059750.
InParanoid	Q969T7.
OMA	QLQGKTN.
OrthoDB	EOG4JWVDW.
PhylomeDB	Q969T7.
Gene expression databases
ArrayExpress	Q969T7.
Bgee	Q969T7.
CleanEx	HS_NT5C3L.
Genevestigator	Q969T7.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR006434. Pyrimidine_nucleotidase_eu. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
PANTHER	PTHR13045. HAD-SF_hydro_IE. 1 hit.
Pfam	PF05822. UMPH-1. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01544. HAD-SF-IE. 1 hit.
ProtoNet	Search...
Other
NextBio	79498.

Entry information

Entry name

5NT3L_HUMAN

Accession

Primary (citable) accession number: Q969T7
Secondary accession number(s): A8MWB9, Q7L3B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.