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Protein

Ubiquitin-conjugating enzyme E2 E3

Gene

UBE2E3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Growth regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS10060-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ969T4.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E3 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme E3
UbcH9
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
Ubiquitin-protein ligase E3
Gene namesi
Name:UBE2E3
Synonyms:UBCE4, UBCH9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:12479. UBE2E3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145C → S: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi10477.
OpenTargetsiENSG00000170035.
PharmGKBiPA37129.

Polymorphism and mutation databases

DMDMi47606197.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000824742 – 207Ubiquitin-conjugating enzyme E2 E3Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei8PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ969T4.
MaxQBiQ969T4.
PaxDbiQ969T4.
PeptideAtlasiQ969T4.
PRIDEiQ969T4.
TopDownProteomicsiQ969T4.

PTM databases

iPTMnetiQ969T4.
PhosphoSitePlusiQ969T4.

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000170035.
CleanExiHS_UBE2E3.
ExpressionAtlasiQ969T4. baseline and differential.
GenevisibleiQ969T4. HS.

Organism-specific databases

HPAiHPA003303.
HPA030445.

Interactioni

Subunit structurei

The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Interacts with NEDD4L.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MID1O153446EBI-348496,EBI-2340316
RNF185Q96GF16EBI-348496,EBI-2340249
RNF5Q999427EBI-348496,EBI-348482
TRIM39Q9HCM94EBI-348496,EBI-739510

Protein-protein interaction databases

BioGridi115740. 98 interactors.
IntActiQ969T4. 64 interactors.
MINTiMINT-1035070.
STRINGi9606.ENSP00000376215.

Structurei

3D structure databases

ProteinModelPortaliQ969T4.
SMRiQ969T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ969T4.
KOiK20217.
OMAiNICEWRS.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ969T4.
TreeFamiTF101117.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q969T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK
60 70 80 90 100
LSSKTTAKLS TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP
110 120 130 140 150
PGSVYEGGVF FLDITFSSDY PFKPPKVTFR TRIYHCNINS QGVICLDILK
160 170 180 190 200
DNWSPALTIS KVLLSICSLL TDCNPADPLV GSIATQYLTN RAEHDRIARQ

WTKRYAT
Length:207
Mass (Da):22,913
Last modified:December 1, 2001 - v1
Checksum:i821CB1382478DC9F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65R → K in AAP97266 (Ref. 3) Curated1
Sequence conflicti91Y → C in AAP97266 (Ref. 3) Curated1
Sequence conflicti101P → L in AAP97266 (Ref. 3) Curated1
Sequence conflicti134Y → D in AAP97266 (Ref. 3) Curated1
Sequence conflicti159I → V in AAP97266 (Ref. 3) Curated1
Sequence conflicti171T → M in AAP97266 (Ref. 3) Curated1
Sequence conflicti176A → V in AAP97266 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023392201W → R.1 PublicationCorresponds to variant rs2368192dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017644 mRNA. Translation: BAA76544.1.
AF085362 mRNA. Translation: AAD40197.1.
AF136176 mRNA. Translation: AAP97266.1.
BT019345 mRNA. Translation: AAV38152.1.
AK314145 mRNA. Translation: BAG36833.1.
AC104076 Genomic DNA. Translation: AAY14882.1.
CH471058 Genomic DNA. Translation: EAX10989.1.
CH471058 Genomic DNA. Translation: EAX10990.1.
BC003554 mRNA. Translation: AAH03554.1.
BC092407 mRNA. Translation: AAH92407.1.
CCDSiCCDS2282.1.
RefSeqiNP_001265483.1. NM_001278554.1.
NP_001265484.1. NM_001278555.1.
NP_006348.1. NM_006357.3.
NP_872619.1. NM_182678.2.
XP_005246301.1. XM_005246244.2.
UniGeneiHs.470804.
Hs.567831.

Genome annotation databases

EnsembliENST00000392415; ENSP00000376215; ENSG00000170035.
ENST00000410062; ENSP00000386788; ENSG00000170035.
ENST00000602710; ENSP00000473623; ENSG00000170035.
ENST00000602959; ENSP00000473639; ENSG00000170035.
GeneIDi10477.
KEGGihsa:10477.
UCSCiuc002unr.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017644 mRNA. Translation: BAA76544.1.
AF085362 mRNA. Translation: AAD40197.1.
AF136176 mRNA. Translation: AAP97266.1.
BT019345 mRNA. Translation: AAV38152.1.
AK314145 mRNA. Translation: BAG36833.1.
AC104076 Genomic DNA. Translation: AAY14882.1.
CH471058 Genomic DNA. Translation: EAX10989.1.
CH471058 Genomic DNA. Translation: EAX10990.1.
BC003554 mRNA. Translation: AAH03554.1.
BC092407 mRNA. Translation: AAH92407.1.
CCDSiCCDS2282.1.
RefSeqiNP_001265483.1. NM_001278554.1.
NP_001265484.1. NM_001278555.1.
NP_006348.1. NM_006357.3.
NP_872619.1. NM_182678.2.
XP_005246301.1. XM_005246244.2.
UniGeneiHs.470804.
Hs.567831.

3D structure databases

ProteinModelPortaliQ969T4.
SMRiQ969T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115740. 98 interactors.
IntActiQ969T4. 64 interactors.
MINTiMINT-1035070.
STRINGi9606.ENSP00000376215.

PTM databases

iPTMnetiQ969T4.
PhosphoSitePlusiQ969T4.

Polymorphism and mutation databases

DMDMi47606197.

Proteomic databases

EPDiQ969T4.
MaxQBiQ969T4.
PaxDbiQ969T4.
PeptideAtlasiQ969T4.
PRIDEiQ969T4.
TopDownProteomicsiQ969T4.

Protocols and materials databases

DNASUi10477.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392415; ENSP00000376215; ENSG00000170035.
ENST00000410062; ENSP00000386788; ENSG00000170035.
ENST00000602710; ENSP00000473623; ENSG00000170035.
ENST00000602959; ENSP00000473639; ENSG00000170035.
GeneIDi10477.
KEGGihsa:10477.
UCSCiuc002unr.3. human.

Organism-specific databases

CTDi10477.
DisGeNETi10477.
GeneCardsiUBE2E3.
H-InvDBHIX0056142.
HGNCiHGNC:12479. UBE2E3.
HPAiHPA003303.
HPA030445.
MIMi604151. gene.
neXtProtiNX_Q969T4.
OpenTargetsiENSG00000170035.
PharmGKBiPA37129.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ969T4.
KOiK20217.
OMAiNICEWRS.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ969T4.
TreeFamiTF101117.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:HS10060-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ969T4.

Miscellaneous databases

ChiTaRSiUBE2E3. human.
GeneWikiiUBE2E3.
GenomeRNAii10477.
PROiQ969T4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170035.
CleanExiHS_UBE2E3.
ExpressionAtlasiQ969T4. baseline and differential.
GenevisibleiQ969T4. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2E3_HUMAN
AccessioniPrimary (citable) accession number: Q969T4
Secondary accession number(s): B2RAD6
, D3DPG3, Q5U0R7, Q7Z4W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.