Reviewed, UniProtKB/Swiss-Prot Q969T4 (UB2E3_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 E3
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase E3
    Ubiquitin carrier protein E3
    Ubiquitin-conjugating enzyme E2-23 kDa
    UbcH9
    UbcM2

Gene names

Name:	UBE2E3
Synonyms:	UBCE4, UBCH9

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus By similarity. Interacts with NEDD4L.
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. Ref.7
Tissue specificity	Ubiquitously expressed at low levels. Highly expressed in skeletal muscle. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Growth regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNF5	Q99942	1	EBI-348496,EBI-348482

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Ubiquitin-conjugating enzyme E2 E3		PRO_0000082474
Sites
	Active site	145	1	Glycyl thioester intermediate
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.8
	Modified residue	91	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	201	1	W → R: dbSNP rs2368192. Ref.3		VAR_023392
Experimental info
	Mutagenesis	145	1	C → S: Loss of activity. Ref.1
	Sequence conflict	65	1	R → K in AAP97266. Ref.3
	Sequence conflict	91	1	Y → C in AAP97266. Ref.3
	Sequence conflict	101	1	P → L in AAP97266. Ref.3
	Sequence conflict	134	1	Y → D in AAP97266. Ref.3
	Sequence conflict	159	1	I → V in AAP97266. Ref.3
	Sequence conflict	171	1	T → M in AAP97266. Ref.3
	Sequence conflict	176	1	A → V in AAP97266. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q969T4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 821CB1382478DC9F	FASTA	207	22,913
	10 20 30 40 50 60 MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK LSSKTTAKLS 70 80 90 100 110 120 TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP PGSVYEGGVF FLDITFSSDY 130 140 150 160 170 180 PFKPPKVTFR TRIYHCNINS QGVICLDILK DNWSPALTIS KVLLSICSLL TDCNPADPLV 190 200 GSIATQYLTN RAEHDRIARQ WTKRYAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme." Ito K., Kato S., Matsuda Y., Kimura M., Okano Y. Cytogenet. Cell Genet. 84:99-104(1999) [PubMed: 10343118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF CYS-145. Tissue: Gastric adenocarcinoma.
[2]	"Human UbcM2 gene, complete cds." Shen Y., Ye M., Fu G., Zhou J., Zhang Q., Huang Q., Xu S., He K., Chen S., Mao M., Chen Z. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical cord blood.
[3]	"Cloning of a new human cDNA homologous to Mus musculus ubiquitin-conjugating enzyme UbcM2." Xin Y.R., Yu L., Zhao S.Y. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-201.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and PNS.
[7]	"Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import." Plafker S.M., Plafker K.S., Weissman A.M., Macara I.G. J. Cell Biol. 167:649-659(2004) [PubMed: 15545318] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB017644 mRNA. Translation: BAA76544.1. AF085362 mRNA. Translation: AAD40197.1. AF136176 mRNA. Translation: AAP97266.1. BT019345 mRNA. Translation: AAV38152.1. AC104076 Genomic DNA. Translation: AAY14882.1. BC003554 mRNA. Translation: AAH03554.1. BC092407 mRNA. Translation: AAH92407.1.
IPI	IPI00056504.
RefSeq	NP_006348.1. NP_872619.1.
UniGene	Hs.470804 Hs.567831
3D structure databases
HSSP	HSSP built from PDB template 1QCQ based on UniProtKB P15731.
SMR	Q969T4. Positions 60-207.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969T4. 2 interactions.
PTM databases
PhosphoSite	Q969T4.
Proteomic databases
PRIDE	Q969T4.
Genome annotation databases
Ensembl	ENSG00000170035. Homo sapiens. [Contig view]
GeneID	10477.
KEGG	hsa:10477.
Organism-specific databases
GeneCards	GC02P181553.
H-InvDB	HIX0002642. HIX0056142.
HGNC	HGNC:12479. UBE2E3.
HPA	HPA003303.
MIM	604151. gene.
PharmGKB	PA37129.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q969T4.
HOVERGEN	Q969T4.
Enzyme and pathway databases
BRENDA	6.3.2.19. 247.
Gene expression databases
CleanEx	HS_UBE2E3.
GermOnline	ENSG00000170035. Homo sapiens.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39740.
SOURCE	Search...

Entry information

Entry name

UB2E3_HUMAN

Accession

Primary (citable) accession number: Q969T4
Secondary accession number(s): Q5U0R7, Q7Z4W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)