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Protein

Ubiquitin-conjugating enzyme E2 E3

Gene

UBE2E3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: Reactome
  • regulation of growth Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
Biological processGrowth regulation, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ969T4
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E3 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme E3
UbcH9
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
Ubiquitin-protein ligase E3
Gene namesi
Name:UBE2E3
Synonyms:UBCE4, UBCH9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000170035.15
HGNCiHGNC:12479 UBE2E3
MIMi604151 gene
neXtProtiNX_Q969T4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145C → S: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi10477
OpenTargetsiENSG00000170035
PharmGKBiPA37129

Polymorphism and mutation databases

DMDMi47606197

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000824742 – 207Ubiquitin-conjugating enzyme E2 E3Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei8PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ969T4
MaxQBiQ969T4
PaxDbiQ969T4
PeptideAtlasiQ969T4
PRIDEiQ969T4
TopDownProteomicsiQ969T4

PTM databases

iPTMnetiQ969T4
PhosphoSitePlusiQ969T4

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000170035
CleanExiHS_UBE2E3
ExpressionAtlasiQ969T4 baseline and differential
GenevisibleiQ969T4 HS

Organism-specific databases

HPAiHPA003303
HPA030445

Interactioni

Subunit structurei

The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Interacts with NEDD4L.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115740, 100 interactors
IntActiQ969T4, 66 interactors
MINTiQ969T4
STRINGi9606.ENSP00000376215

Structurei

3D structure databases

ProteinModelPortaliQ969T4
SMRiQ969T4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiQ969T4
KOiK20217
OMAiGDRAKHD
OrthoDBiEOG091G0GF8
PhylomeDBiQ969T4
TreeFamiTF101117

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q969T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK
60 70 80 90 100
LSSKTTAKLS TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP
110 120 130 140 150
PGSVYEGGVF FLDITFSSDY PFKPPKVTFR TRIYHCNINS QGVICLDILK
160 170 180 190 200
DNWSPALTIS KVLLSICSLL TDCNPADPLV GSIATQYLTN RAEHDRIARQ

WTKRYAT
Length:207
Mass (Da):22,913
Last modified:December 1, 2001 - v1
Checksum:i821CB1382478DC9F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65R → K in AAP97266 (Ref. 3) Curated1
Sequence conflicti91Y → C in AAP97266 (Ref. 3) Curated1
Sequence conflicti101P → L in AAP97266 (Ref. 3) Curated1
Sequence conflicti134Y → D in AAP97266 (Ref. 3) Curated1
Sequence conflicti159I → V in AAP97266 (Ref. 3) Curated1
Sequence conflicti171T → M in AAP97266 (Ref. 3) Curated1
Sequence conflicti176A → V in AAP97266 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023392201W → R1 PublicationCorresponds to variant dbSNP:rs2368192Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017644 mRNA Translation: BAA76544.1
AF085362 mRNA Translation: AAD40197.1
AF136176 mRNA Translation: AAP97266.1
BT019345 mRNA Translation: AAV38152.1
AK314145 mRNA Translation: BAG36833.1
AC104076 Genomic DNA Translation: AAY14882.1
CH471058 Genomic DNA Translation: EAX10989.1
CH471058 Genomic DNA Translation: EAX10990.1
BC003554 mRNA Translation: AAH03554.1
BC092407 mRNA Translation: AAH92407.1
CCDSiCCDS2282.1
RefSeqiNP_001265483.1, NM_001278554.1
NP_001265484.1, NM_001278555.1
NP_006348.1, NM_006357.3
NP_872619.1, NM_182678.2
XP_005246301.1, XM_005246244.2
UniGeneiHs.470804
Hs.567831

Genome annotation databases

EnsembliENST00000392415; ENSP00000376215; ENSG00000170035
ENST00000410062; ENSP00000386788; ENSG00000170035
ENST00000602710; ENSP00000473623; ENSG00000170035
ENST00000602959; ENSP00000473639; ENSG00000170035
GeneIDi10477
KEGGihsa:10477
UCSCiuc002unr.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiUB2E3_HUMAN
AccessioniPrimary (citable) accession number: Q969T4
Secondary accession number(s): B2RAD6
, D3DPG3, Q5U0R7, Q7Z4W4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

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