Q969T4 (UB2E3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 E3
EC=6.3.2.19

Alternative name(s):
UbcH9
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
Ubiquitin-protein ligase E3

Gene names

Name:	UBE2E3
Synonyms:	UBCE4, UBCH9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Ref.1 Ref.10
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus By similarity. Interacts with NEDD4L.
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. Ref.9
Tissue specificity	Ubiquitously expressed at low levels. Highly expressed in skeletal muscle. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Growth regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K11-linked ubiquitination Inferred from direct assay Ref.10. Source: UniProtKB protein K48-linked ubiquitination Inferred from direct assay Ref.10. Source: UniProtKB protein K63-linked ubiquitination Inferred from direct assay Ref.10. Source: UniProtKB regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.10. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Ubiquitin-conjugating enzyme E2 E3		PRO_0000082474
Sites
	Active site	145	1	Glycyl thioester intermediate
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.12
	Modified residue	91	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	201	1	W → R. Ref.3 Corresponds to variant rs2368192 [ dbSNP | Ensembl ].		VAR_023392
Experimental info
	Mutagenesis	145	1	C → S: Loss of activity. Ref.1
	Sequence conflict	65	1	R → K in AAP97266. Ref.3
	Sequence conflict	91	1	Y → C in AAP97266. Ref.3
	Sequence conflict	101	1	P → L in AAP97266. Ref.3
	Sequence conflict	134	1	Y → D in AAP97266. Ref.3
	Sequence conflict	159	1	I → V in AAP97266. Ref.3
	Sequence conflict	171	1	T → M in AAP97266. Ref.3
	Sequence conflict	176	1	A → V in AAP97266. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q969T4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 821CB1382478DC9F	FASTA	207	22,913
	10 20 30 40 50 60 MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK LSSKTTAKLS 70 80 90 100 110 120 TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP PGSVYEGGVF FLDITFSSDY 130 140 150 160 170 180 PFKPPKVTFR TRIYHCNINS QGVICLDILK DNWSPALTIS KVLLSICSLL TDCNPADPLV 190 200 GSIATQYLTN RAEHDRIARQ WTKRYAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme." Ito K., Kato S., Matsuda Y., Kimura M., Okano Y. Cytogenet. Cell Genet. 84:99-104(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF CYS-145. Tissue: Gastric adenocarcinoma.
[2]	"Human UbcM2 gene, complete cds." Shen Y., Ye M., Fu G., Zhou J., Zhang Q., Huang Q., Xu S., He K., Chen S., Mao M., Chen Z. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical cord blood.
[3]	"Cloning of a new human cDNA homologous to Mus musculus ubiquitin-conjugating enzyme UbcM2." Xin Y.R., Yu L., Zhao S.Y. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-201.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and PNS.
[9]	"Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import." Plafker S.M., Plafker K.S., Weissman A.M., Macara I.G. J. Cell Biol. 167:649-659(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines." David Y., Ziv T., Admon A., Navon A. J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017644 mRNA. Translation: BAA76544.1. AF085362 mRNA. Translation: AAD40197.1. AF136176 mRNA. Translation: AAP97266.1. BT019345 mRNA. Translation: AAV38152.1. AK314145 mRNA. Translation: BAG36833.1. AC104076 Genomic DNA. Translation: AAY14882.1. CH471058 Genomic DNA. Translation: EAX10989.1. CH471058 Genomic DNA. Translation: EAX10990.1. BC003554 mRNA. Translation: AAH03554.1. BC092407 mRNA. Translation: AAH92407.1.
IPI	IPI00056504.
RefSeq	NP_006348.1. NM_006357.2. NP_872619.1. NM_182678.1.
UniGene	Hs.470804. Hs.567831.
3D structure databases
ProteinModelPortal	Q969T4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969T4. 55 interactions.
MINT	MINT-1035070.
STRING	9606.ENSP00000307109.
PTM databases
PhosphoSite	Q969T4.
Polymorphism databases
DMDM	47606197.
Proteomic databases
PaxDb	Q969T4.
PRIDE	Q969T4.
Protocols and materials databases
DNASU	10477.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000392415; ENSP00000376215; ENSG00000170035. ENST00000410062; ENSP00000386788; ENSG00000170035.
GeneID	10477.
KEGG	hsa:10477.
UCSC	uc002unq.1. human.
Organism-specific databases
CTD	10477.
GeneCards	GC02P181809.
H-InvDB	HIX0056142.
HGNC	HGNC:12479. UBE2E3.
HPA	HPA003303. HPA030445.
MIM	604151. gene.
neXtProt	NX_Q969T4.
PharmGKB	PA37129.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
KO	K06689.
OMA	AKPNPKM.
PhylomeDB	Q969T4.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q969T4.
Bgee	Q969T4.
CleanEx	HS_UBE2E3.
Genevestigator	Q969T4.
GermOnline	ENSG00000170035. Homo sapiens.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	UBE2E3. human.
GenomeRNAi	10477.
NextBio	39740.
SOURCE	Search...

Entry information

Entry name

UB2E3_HUMAN

Accession

Primary (citable) accession number: Q969T4
Secondary accession number(s): B2RAD6 , D3DPG3, Q5U0R7, Q7Z4W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.