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Q969T4

- UB2E3_HUMAN

UniProt

Q969T4 - UB2E3_HUMAN

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Protein

Ubiquitin-conjugating enzyme E2 E3

Gene

UBE2E3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei145 – 1451Glycyl thioester intermediate

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein K11-linked ubiquitination Source: UniProtKB
  2. protein K48-linked ubiquitination Source: UniProtKB
  3. protein K63-linked ubiquitination Source: UniProtKB
  4. regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Growth regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ969T4.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E3 (EC:6.3.2.19)
Alternative name(s):
UbcH9
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
Ubiquitin-protein ligase E3
Gene namesi
Name:UBE2E3
Synonyms:UBCE4, UBCH9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:12479. UBE2E3.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi145 – 1451C → S: Loss of activity. 1 Publication

Organism-specific databases

PharmGKBiPA37129.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 207206Ubiquitin-conjugating enzyme E2 E3PRO_0000082474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei8 – 81Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ969T4.
PaxDbiQ969T4.
PRIDEiQ969T4.

PTM databases

PhosphoSiteiQ969T4.

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.1 Publication

Gene expression databases

BgeeiQ969T4.
CleanExiHS_UBE2E3.
ExpressionAtlasiQ969T4. baseline.
GenevestigatoriQ969T4.

Organism-specific databases

HPAiHPA003303.
HPA030445.

Interactioni

Subunit structurei

The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus (By similarity). Interacts with NEDD4L.By similarity

Protein-protein interaction databases

BioGridi115740. 86 interactions.
IntActiQ969T4. 56 interactions.
MINTiMINT-1035070.
STRINGi9606.ENSP00000307109.

Structurei

3D structure databases

ProteinModelPortaliQ969T4.
SMRiQ969T4. Positions 60-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ969T4.
KOiK06689.
OMAiAKPNPKM.
OrthoDBiEOG7PCJGX.
PhylomeDBiQ969T4.
TreeFamiTF101117.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q969T4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK
60 70 80 90 100
LSSKTTAKLS TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP
110 120 130 140 150
PGSVYEGGVF FLDITFSSDY PFKPPKVTFR TRIYHCNINS QGVICLDILK
160 170 180 190 200
DNWSPALTIS KVLLSICSLL TDCNPADPLV GSIATQYLTN RAEHDRIARQ

WTKRYAT
Length:207
Mass (Da):22,913
Last modified:December 1, 2001 - v1
Checksum:i821CB1382478DC9F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651R → K in AAP97266. 1 PublicationCurated
Sequence conflicti91 – 911Y → C in AAP97266. 1 PublicationCurated
Sequence conflicti101 – 1011P → L in AAP97266. 1 PublicationCurated
Sequence conflicti134 – 1341Y → D in AAP97266. 1 PublicationCurated
Sequence conflicti159 – 1591I → V in AAP97266. 1 PublicationCurated
Sequence conflicti171 – 1711T → M in AAP97266. 1 PublicationCurated
Sequence conflicti176 – 1761A → V in AAP97266. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti201 – 2011W → R.1 Publication
Corresponds to variant rs2368192 [ dbSNP | Ensembl ].
VAR_023392

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017644 mRNA. Translation: BAA76544.1.
AF085362 mRNA. Translation: AAD40197.1.
AF136176 mRNA. Translation: AAP97266.1.
BT019345 mRNA. Translation: AAV38152.1.
AK314145 mRNA. Translation: BAG36833.1.
AC104076 Genomic DNA. Translation: AAY14882.1.
CH471058 Genomic DNA. Translation: EAX10989.1.
CH471058 Genomic DNA. Translation: EAX10990.1.
BC003554 mRNA. Translation: AAH03554.1.
BC092407 mRNA. Translation: AAH92407.1.
CCDSiCCDS2282.1.
RefSeqiNP_001265483.1. NM_001278554.1.
NP_001265484.1. NM_001278555.1.
NP_006348.1. NM_006357.3.
NP_872619.1. NM_182678.2.
XP_005246301.1. XM_005246244.2.
UniGeneiHs.470804.
Hs.567831.

Genome annotation databases

EnsembliENST00000392415; ENSP00000376215; ENSG00000170035.
ENST00000410062; ENSP00000386788; ENSG00000170035.
ENST00000602710; ENSP00000473623; ENSG00000170035.
ENST00000602959; ENSP00000473639; ENSG00000170035.
GeneIDi10477.
KEGGihsa:10477.
UCSCiuc002unq.1. human.

Polymorphism databases

DMDMi47606197.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017644 mRNA. Translation: BAA76544.1 .
AF085362 mRNA. Translation: AAD40197.1 .
AF136176 mRNA. Translation: AAP97266.1 .
BT019345 mRNA. Translation: AAV38152.1 .
AK314145 mRNA. Translation: BAG36833.1 .
AC104076 Genomic DNA. Translation: AAY14882.1 .
CH471058 Genomic DNA. Translation: EAX10989.1 .
CH471058 Genomic DNA. Translation: EAX10990.1 .
BC003554 mRNA. Translation: AAH03554.1 .
BC092407 mRNA. Translation: AAH92407.1 .
CCDSi CCDS2282.1.
RefSeqi NP_001265483.1. NM_001278554.1.
NP_001265484.1. NM_001278555.1.
NP_006348.1. NM_006357.3.
NP_872619.1. NM_182678.2.
XP_005246301.1. XM_005246244.2.
UniGenei Hs.470804.
Hs.567831.

3D structure databases

ProteinModelPortali Q969T4.
SMRi Q969T4. Positions 60-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115740. 86 interactions.
IntActi Q969T4. 56 interactions.
MINTi MINT-1035070.
STRINGi 9606.ENSP00000307109.

PTM databases

PhosphoSitei Q969T4.

Polymorphism databases

DMDMi 47606197.

Proteomic databases

MaxQBi Q969T4.
PaxDbi Q969T4.
PRIDEi Q969T4.

Protocols and materials databases

DNASUi 10477.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000392415 ; ENSP00000376215 ; ENSG00000170035 .
ENST00000410062 ; ENSP00000386788 ; ENSG00000170035 .
ENST00000602710 ; ENSP00000473623 ; ENSG00000170035 .
ENST00000602959 ; ENSP00000473639 ; ENSG00000170035 .
GeneIDi 10477.
KEGGi hsa:10477.
UCSCi uc002unq.1. human.

Organism-specific databases

CTDi 10477.
GeneCardsi GC02P181809.
H-InvDB HIX0056142.
HGNCi HGNC:12479. UBE2E3.
HPAi HPA003303.
HPA030445.
MIMi 604151. gene.
neXtProti NX_Q969T4.
PharmGKBi PA37129.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00760000119012.
HOGENOMi HOG000233455.
HOVERGENi HBG063308.
InParanoidi Q969T4.
KOi K06689.
OMAi AKPNPKM.
OrthoDBi EOG7PCJGX.
PhylomeDBi Q969T4.
TreeFami TF101117.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinki Q969T4.

Miscellaneous databases

ChiTaRSi UBE2E3. human.
GeneWikii UBE2E3.
GenomeRNAii 10477.
NextBioi 39740.
PROi Q969T4.
SOURCEi Search...

Gene expression databases

Bgeei Q969T4.
CleanExi HS_UBE2E3.
ExpressionAtlasi Q969T4. baseline.
Genevestigatori Q969T4.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme."
    Ito K., Kato S., Matsuda Y., Kimura M., Okano Y.
    Cytogenet. Cell Genet. 84:99-104(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF CYS-145.
    Tissue: Gastric adenocarcinoma.
  2. "Human UbcM2 gene, complete cds."
    Shen Y., Ye M., Fu G., Zhou J., Zhang Q., Huang Q., Xu S., He K., Chen S., Mao M., Chen Z.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Umbilical cord blood.
  3. "Cloning of a new human cDNA homologous to Mus musculus ubiquitin-conjugating enzyme UbcM2."
    Xin Y.R., Yu L., Zhao S.Y.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-201.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and PNS.
  9. "Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import."
    Plafker S.M., Plafker K.S., Weissman A.M., Macara I.G.
    J. Cell Biol. 167:649-659(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUB2E3_HUMAN
AccessioniPrimary (citable) accession number: Q969T4
Secondary accession number(s): B2RAD6
, D3DPG3, Q5U0R7, Q7Z4W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3