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Protein

Ribosome-releasing factor 2, mitochondrial

Gene

GFM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.UniRule annotation1 Publication

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84GTPUniRule annotation8
Nucleotide bindingi141 – 145GTPUniRule annotation5
Nucleotide bindingi195 – 198GTPUniRule annotation4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • mitochondrial translation Source: UniProtKB
  • mitochondrial translational termination Source: Reactome
  • ribosome disassembly Source: UniProtKB

Keywordsi

Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5419276 Mitochondrial translation termination

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-releasing factor 2, mitochondrialUniRule annotation
Short name:
RRF2mtUniRule annotation
Alternative name(s):
Elongation factor G 2, mitochondrialUniRule annotation
Short name:
EF-G2mtUniRule annotation
Short name:
mEF-G 2UniRule annotation
Elongation factor G2
Short name:
hEFG2
Gene namesi
Name:GFM2UniRule annotation
Synonyms:EFG2UniRule annotation
ORF Names:MSTP027
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164347.17
HGNCiHGNC:29682 GFM2
MIMi606544 gene
neXtProtiNX_Q969S9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

MalaCardsiGFM2
OpenTargetsiENSG00000164347
PharmGKBiPA134949527

Polymorphism and mutation databases

BioMutaiGFM2
DMDMi28201798

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000074501 – 779Ribosome-releasing factor 2, mitochondrialAdd BLAST779

Proteomic databases

EPDiQ969S9
MaxQBiQ969S9
PaxDbiQ969S9
PeptideAtlasiQ969S9
PRIDEiQ969S9

PTM databases

CarbonylDBiQ969S9
iPTMnetiQ969S9
PhosphoSitePlusiQ969S9

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000164347
CleanExiHS_GFM2
ExpressionAtlasiQ969S9 baseline and differential
GenevisibleiQ969S9 HS

Organism-specific databases

HPAiHPA036863

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi124062, 22 interactors
DIPiDIP-53437N
IntActiQ969S9, 9 interactors
MINTiQ969S9
STRINGi9606.ENSP00000296805

Structurei

3D structure databases

ProteinModelPortaliQ969S9
SMRiQ969S9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 353tr-type GAdd BLAST286

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0465 Eukaryota
COG0480 LUCA
GeneTreeiENSGT00550000074890
HOGENOMiHOG000231585
InParanoidiQ969S9
KOiK02355
OMAiQGMDDMV
OrthoDBiEOG091G02HJ
PhylomeDBiQ969S9
TreeFamiTF314848

Family and domain databases

CDDicd16262 EFG_III, 1 hit
cd03713 EFG_mtEFG_C, 1 hit
Gene3Di3.30.230.10, 2 hits
HAMAPiMF_03059 mEF_G_2, 1 hit
InterProiView protein in InterPro
IPR030851 EFG2
IPR009022 EFG_III
IPR035647 EFG_III/V
IPR035649 EFG_V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTNLRIFAM SHQTIPSVYI NNICCYKIRA SLKRLKPHVP LGRNCSSLPG
60 70 80 90 100
LIGNDIKSLH SIINPPIAKI RNIGIMAHID AGKTTTTERI LYYSGYTRSL
110 120 130 140 150
GDVDDGDTVT DFMAQERERG ITIQSAAVTF DWKGYRVNLI DTPGHVDFTL
160 170 180 190 200
EVERCLRVLD GAVAVFDASA GVEAQTLTVW RQADKHNIPR ICFLNKMDKT
210 220 230 240 250
GASFKYAVES IREKLKAKPL LLQLPIGEAK TFKGVVDVVM KEKLLWNCNS
260 270 280 290 300
NDGKDFERKP LLEMNDPELL KETTEARNAL IEQVADLDDE FADLVLEEFS
310 320 330 340 350
ENFDLLPAEK LQTAIHRVTL AQTAVPVLCG SALKNKGIQP LLDAVTMYLP
360 370 380 390 400
SPEERNYEFL QWYKDDLCAL AFKVLHDKQR GPLVFMRIYS GTIKPQLAIH
410 420 430 440 450
NINGNCTERI SRLLLPFADQ HVEIPSLTAG NIALTVGLKH TATGDTIVSS
460 470 480 490 500
KSSALAAARR AEREGEKKHR QNNEAERLLL AGVEIPEPVF FCTIEPPSLS
510 520 530 540 550
KQPDLEHALK CLQREDPSLK VRLDPDSGQT VLCGMGELHI EIIHDRIKRE
560 570 580 590 600
YGLETYLGPL QVAYRETILN SVRATDTLDR TLGDKRHLVT VEVEARPIET
610 620 630 640 650
SSVMPVIEFE YAESINEGLL KVSQEAIENG IHSACLQGPL LGSPIQDVAI
660 670 680 690 700
TLHSLTIHPG TSTTMISACV SRCVQKALKK ADKQVLEPLM NLEVTVARDY
710 720 730 740 750
LSPVLADLAQ RRGNIQEIQT RQDNKVVIGF VPLAEIMGYS TVLRTLTSGS
760 770
ATFALELSTY QAMNPQDQNT LLNRRSGLT
Length:779
Mass (Da):86,601
Last modified:December 1, 2001 - v1
Checksum:i856B4E26FD9DD94E
GO
Isoform 2 (identifier: Q969S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-407: Missing.

Note: No experimental confirmation available.
Show »
Length:732
Mass (Da):81,200
Checksum:i15869E090CB0848A
GO
Isoform 3 (identifier: Q969S9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     608-609: Missing.

Note: No experimental confirmation available.
Show »
Length:777
Mass (Da):86,324
Checksum:i539970B070FB4904
GO
Isoform 4 (identifier: Q969S9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     530-531: TV → IP
     532-709: Missing.

Note: No experimental confirmation available.
Show »
Length:601
Mass (Da):67,068
Checksum:iC712D5773F21E83E
GO
Isoform 5 (identifier: Q969S9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-513: DLEHALKCLQ → GINGLSVSTN
     514-779: Missing.

Note: No experimental confirmation available.
Show »
Length:513
Mass (Da):57,060
Checksum:i81B3AF2019E9D71F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59L → I in AAH30612 (PubMed:15489334).Curated1
Sequence conflicti201G → V in AAS49035 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05398364N → S. Corresponds to variant dbSNP:rs957680EnsemblClinVar.1
Natural variantiVAR_053984300S → C. Corresponds to variant dbSNP:rs16872235EnsemblClinVar.1
Natural variantiVAR_060200425P → H1 PublicationCorresponds to variant dbSNP:rs17852780Ensembl.1
Natural variantiVAR_060201594E → G1 PublicationCorresponds to variant dbSNP:rs17856872Ensembl.1
Natural variantiVAR_060202735E → G1 PublicationCorresponds to variant dbSNP:rs17856871Ensembl.1
Natural variantiVAR_053985774R → Q. Corresponds to variant dbSNP:rs1048167EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001363361 – 407Missing in isoform 2. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_038190504 – 513DLEHALKCLQ → GINGLSVSTN in isoform 5. 1 Publication10
Alternative sequenceiVSP_038191514 – 779Missing in isoform 5. 1 PublicationAdd BLAST266
Alternative sequenceiVSP_038192530 – 531TV → IP in isoform 4. 1 Publication2
Alternative sequenceiVSP_038193532 – 709Missing in isoform 4. 1 PublicationAdd BLAST178
Alternative sequenceiVSP_038194608 – 609Missing in isoform 3. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367997 mRNA Translation: AAK53401.1
AY453587 mRNA Translation: AAS49035.1
AK025314 mRNA Translation: BAB15109.1
AF111808 mRNA Translation: AAL39010.1
CH471084 Genomic DNA Translation: EAW95744.1
BC015712 mRNA Translation: AAH15712.1
BC030612 mRNA Translation: AAH30612.1
CCDSiCCDS4023.1 [Q969S9-1]
CCDS4024.1 [Q969S9-2]
CCDS47232.1 [Q969S9-5]
RefSeqiNP_001268231.1, NM_001281302.1
NP_115756.2, NM_032380.4 [Q969S9-1]
NP_733781.1, NM_170681.2 [Q969S9-5]
NP_733792.1, NM_170691.2 [Q969S9-2]
XP_016865475.1, XM_017009986.1 [Q969S9-1]
UniGeneiHs.277154
Hs.656975

Genome annotation databases

EnsembliENST00000296805; ENSP00000296805; ENSG00000164347 [Q969S9-1]
ENST00000345239; ENSP00000296804; ENSG00000164347 [Q969S9-2]
ENST00000427854; ENSP00000405808; ENSG00000164347 [Q969S9-5]
ENST00000509430; ENSP00000427004; ENSG00000164347 [Q969S9-1]
GeneIDi84340
KEGGihsa:84340
UCSCiuc003kdh.2 human [Q969S9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRRF2M_HUMAN
AccessioniPrimary (citable) accession number: Q969S9
Secondary accession number(s): A0AR28
, Q8N6D8, Q8WYI0, Q9H6Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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