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Protein

Ribosome-releasing factor 2, mitochondrial

Gene

GFM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84GTPUniRule annotation8
Nucleotide bindingi141 – 145GTPUniRule annotation5
Nucleotide bindingi195 – 198GTPUniRule annotation4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • mitochondrial translation Source: UniProtKB
  • mitochondrial translational termination Source: Reactome
  • ribosome disassembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164347-MONOMER.
ReactomeiR-HSA-5419276. Mitochondrial translation termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-releasing factor 2, mitochondrialUniRule annotation
Short name:
RRF2mtUniRule annotation
Alternative name(s):
Elongation factor G 2, mitochondrialUniRule annotation
Short name:
EF-G2mtUniRule annotation
Short name:
mEF-G 2UniRule annotation
Elongation factor G2
Short name:
hEFG2
Gene namesi
Name:GFM2UniRule annotation
Synonyms:EFG2UniRule annotation
ORF Names:MSTP027
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:29682. GFM2.

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000164347.
PharmGKBiPA134949527.

Polymorphism and mutation databases

BioMutaiGFM2.
DMDMi28201798.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000007450? – 779Ribosome-releasing factor 2, mitochondrial
Transit peptidei1 – ?MitochondrionUniRule annotation

Proteomic databases

EPDiQ969S9.
MaxQBiQ969S9.
PaxDbiQ969S9.
PeptideAtlasiQ969S9.
PRIDEiQ969S9.

PTM databases

iPTMnetiQ969S9.
PhosphoSitePlusiQ969S9.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000164347.
CleanExiHS_GFM2.
ExpressionAtlasiQ969S9. baseline and differential.
GenevisibleiQ969S9. HS.

Organism-specific databases

HPAiHPA036863.
HPA036864.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BANPQ8N9N53EBI-2371750,EBI-744695
TRIM27P143733EBI-2371750,EBI-719493
TRIM9Q9C0263EBI-2371750,EBI-720828
VPS52Q8N1B43EBI-2371750,EBI-2799833

Protein-protein interaction databases

BioGridi124062. 17 interactors.
IntActiQ969S9. 8 interactors.
STRINGi9606.ENSP00000296805.

Structurei

3D structure databases

ProteinModelPortaliQ969S9.
SMRiQ969S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 353tr-type GAdd BLAST286

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0465. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00550000074890.
HOGENOMiHOG000231585.
InParanoidiQ969S9.
KOiK02355.
OMAiNCLQRED.
OrthoDBiEOG091G02HJ.
PhylomeDBiQ969S9.
TreeFamiTF314848.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03059. mEF_G_2. 1 hit.
InterProiIPR030851. EFG2.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTNLRIFAM SHQTIPSVYI NNICCYKIRA SLKRLKPHVP LGRNCSSLPG
60 70 80 90 100
LIGNDIKSLH SIINPPIAKI RNIGIMAHID AGKTTTTERI LYYSGYTRSL
110 120 130 140 150
GDVDDGDTVT DFMAQERERG ITIQSAAVTF DWKGYRVNLI DTPGHVDFTL
160 170 180 190 200
EVERCLRVLD GAVAVFDASA GVEAQTLTVW RQADKHNIPR ICFLNKMDKT
210 220 230 240 250
GASFKYAVES IREKLKAKPL LLQLPIGEAK TFKGVVDVVM KEKLLWNCNS
260 270 280 290 300
NDGKDFERKP LLEMNDPELL KETTEARNAL IEQVADLDDE FADLVLEEFS
310 320 330 340 350
ENFDLLPAEK LQTAIHRVTL AQTAVPVLCG SALKNKGIQP LLDAVTMYLP
360 370 380 390 400
SPEERNYEFL QWYKDDLCAL AFKVLHDKQR GPLVFMRIYS GTIKPQLAIH
410 420 430 440 450
NINGNCTERI SRLLLPFADQ HVEIPSLTAG NIALTVGLKH TATGDTIVSS
460 470 480 490 500
KSSALAAARR AEREGEKKHR QNNEAERLLL AGVEIPEPVF FCTIEPPSLS
510 520 530 540 550
KQPDLEHALK CLQREDPSLK VRLDPDSGQT VLCGMGELHI EIIHDRIKRE
560 570 580 590 600
YGLETYLGPL QVAYRETILN SVRATDTLDR TLGDKRHLVT VEVEARPIET
610 620 630 640 650
SSVMPVIEFE YAESINEGLL KVSQEAIENG IHSACLQGPL LGSPIQDVAI
660 670 680 690 700
TLHSLTIHPG TSTTMISACV SRCVQKALKK ADKQVLEPLM NLEVTVARDY
710 720 730 740 750
LSPVLADLAQ RRGNIQEIQT RQDNKVVIGF VPLAEIMGYS TVLRTLTSGS
760 770
ATFALELSTY QAMNPQDQNT LLNRRSGLT
Length:779
Mass (Da):86,601
Last modified:December 1, 2001 - v1
Checksum:i856B4E26FD9DD94E
GO
Isoform 2 (identifier: Q969S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-407: Missing.

Note: No experimental confirmation available.
Show »
Length:732
Mass (Da):81,200
Checksum:i15869E090CB0848A
GO
Isoform 3 (identifier: Q969S9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     608-609: Missing.

Note: No experimental confirmation available.
Show »
Length:777
Mass (Da):86,324
Checksum:i539970B070FB4904
GO
Isoform 4 (identifier: Q969S9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     530-531: TV → IP
     532-709: Missing.

Note: No experimental confirmation available.
Show »
Length:601
Mass (Da):67,068
Checksum:iC712D5773F21E83E
GO
Isoform 5 (identifier: Q969S9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-513: DLEHALKCLQ → GINGLSVSTN
     514-779: Missing.

Note: No experimental confirmation available.
Show »
Length:513
Mass (Da):57,060
Checksum:i81B3AF2019E9D71F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59L → I in AAH30612 (PubMed:15489334).Curated1
Sequence conflicti201G → V in AAS49035 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05398364N → S.Corresponds to variant rs957680dbSNPEnsembl.1
Natural variantiVAR_053984300S → C.Corresponds to variant rs16872235dbSNPEnsembl.1
Natural variantiVAR_060200425P → H.1 PublicationCorresponds to variant rs17852780dbSNPEnsembl.1
Natural variantiVAR_060201594E → G.1 PublicationCorresponds to variant rs17856872dbSNPEnsembl.1
Natural variantiVAR_060202735E → G.1 PublicationCorresponds to variant rs17856871dbSNPEnsembl.1
Natural variantiVAR_053985774R → Q.Corresponds to variant rs1048167dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001363361 – 407Missing in isoform 2. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_038190504 – 513DLEHALKCLQ → GINGLSVSTN in isoform 5. 1 Publication10
Alternative sequenceiVSP_038191514 – 779Missing in isoform 5. 1 PublicationAdd BLAST266
Alternative sequenceiVSP_038192530 – 531TV → IP in isoform 4. 1 Publication2
Alternative sequenceiVSP_038193532 – 709Missing in isoform 4. 1 PublicationAdd BLAST178
Alternative sequenceiVSP_038194608 – 609Missing in isoform 3. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367997 mRNA. Translation: AAK53401.1.
AY453587 mRNA. Translation: AAS49035.1.
AK025314 mRNA. Translation: BAB15109.1.
AF111808 mRNA. Translation: AAL39010.1.
CH471084 Genomic DNA. Translation: EAW95744.1.
BC015712 mRNA. Translation: AAH15712.1.
BC030612 mRNA. Translation: AAH30612.1.
CCDSiCCDS4023.1. [Q969S9-1]
CCDS4024.1. [Q969S9-2]
CCDS47232.1. [Q969S9-5]
RefSeqiNP_001268231.1. NM_001281302.1.
NP_115756.2. NM_032380.4. [Q969S9-1]
NP_733781.1. NM_170681.2. [Q969S9-5]
NP_733792.1. NM_170691.2. [Q969S9-2]
XP_016865475.1. XM_017009986.1. [Q969S9-1]
UniGeneiHs.277154.
Hs.656975.

Genome annotation databases

EnsembliENST00000296805; ENSP00000296805; ENSG00000164347. [Q969S9-1]
ENST00000345239; ENSP00000296804; ENSG00000164347. [Q969S9-2]
ENST00000427854; ENSP00000405808; ENSG00000164347. [Q969S9-5]
ENST00000509430; ENSP00000427004; ENSG00000164347. [Q969S9-1]
GeneIDi84340.
KEGGihsa:84340.
UCSCiuc003kdh.2. human. [Q969S9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367997 mRNA. Translation: AAK53401.1.
AY453587 mRNA. Translation: AAS49035.1.
AK025314 mRNA. Translation: BAB15109.1.
AF111808 mRNA. Translation: AAL39010.1.
CH471084 Genomic DNA. Translation: EAW95744.1.
BC015712 mRNA. Translation: AAH15712.1.
BC030612 mRNA. Translation: AAH30612.1.
CCDSiCCDS4023.1. [Q969S9-1]
CCDS4024.1. [Q969S9-2]
CCDS47232.1. [Q969S9-5]
RefSeqiNP_001268231.1. NM_001281302.1.
NP_115756.2. NM_032380.4. [Q969S9-1]
NP_733781.1. NM_170681.2. [Q969S9-5]
NP_733792.1. NM_170691.2. [Q969S9-2]
XP_016865475.1. XM_017009986.1. [Q969S9-1]
UniGeneiHs.277154.
Hs.656975.

3D structure databases

ProteinModelPortaliQ969S9.
SMRiQ969S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124062. 17 interactors.
IntActiQ969S9. 8 interactors.
STRINGi9606.ENSP00000296805.

PTM databases

iPTMnetiQ969S9.
PhosphoSitePlusiQ969S9.

Polymorphism and mutation databases

BioMutaiGFM2.
DMDMi28201798.

Proteomic databases

EPDiQ969S9.
MaxQBiQ969S9.
PaxDbiQ969S9.
PeptideAtlasiQ969S9.
PRIDEiQ969S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296805; ENSP00000296805; ENSG00000164347. [Q969S9-1]
ENST00000345239; ENSP00000296804; ENSG00000164347. [Q969S9-2]
ENST00000427854; ENSP00000405808; ENSG00000164347. [Q969S9-5]
ENST00000509430; ENSP00000427004; ENSG00000164347. [Q969S9-1]
GeneIDi84340.
KEGGihsa:84340.
UCSCiuc003kdh.2. human. [Q969S9-1]

Organism-specific databases

CTDi84340.
GeneCardsiGFM2.
HGNCiHGNC:29682. GFM2.
HPAiHPA036863.
HPA036864.
MIMi606544. gene.
neXtProtiNX_Q969S9.
OpenTargetsiENSG00000164347.
PharmGKBiPA134949527.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0465. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00550000074890.
HOGENOMiHOG000231585.
InParanoidiQ969S9.
KOiK02355.
OMAiNCLQRED.
OrthoDBiEOG091G02HJ.
PhylomeDBiQ969S9.
TreeFamiTF314848.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164347-MONOMER.
ReactomeiR-HSA-5419276. Mitochondrial translation termination.

Miscellaneous databases

ChiTaRSiGFM2. human.
GenomeRNAii84340.
PROiQ969S9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164347.
CleanExiHS_GFM2.
ExpressionAtlasiQ969S9. baseline and differential.
GenevisibleiQ969S9. HS.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03059. mEF_G_2. 1 hit.
InterProiIPR030851. EFG2.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRRF2M_HUMAN
AccessioniPrimary (citable) accession number: Q969S9
Secondary accession number(s): A0AR28
, Q8N6D8, Q8WYI0, Q9H6Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.