Q969S0 (S35B4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-xylose and UDP-N-acetylglucosamine transporter

Alternative name(s):
Solute carrier family 35 member B4
YEA4 homolog

Gene names

Name:	SLC35B4
Synonyms:	YEA4
ORF Names:	PSEC0055

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sugar transporter that specifically mediates the transport of UDP-xylose (UDP-Xyl) and UDP-N-acetylglucosamine (UDP-GlcNAc) from cytosol into Golgi. Ref.1
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.1.
Sequence similarities	Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.
Sequence caution	The sequence BAB55325.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UDP-N-acetylglucosamine transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB UDP-xylose transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q969S0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q969S0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-231: ELYEIPV → GEFGVRQ
     232-331: Missing.

Isoform 3 (identifier: Q969S0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     251-253: YVC → TPT
     254-331: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-xylose and UDP-N-acetylglucosamine transporter		PRO_0000213385
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	251 – 267	17	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Transmembrane	291 – 311	21	Helical; Potential
Natural variations
	Alternative sequence	225 – 231	7	ELYEIPV → GEFGVRQ in isoform 2.		VSP_016197
	Alternative sequence	232 – 331	100	Missing in isoform 2.		VSP_016198
	Alternative sequence	251 – 253	3	YVC → TPT in isoform 3.		VSP_016199
	Alternative sequence	254 – 331	78	Missing in isoform 3.		VSP_016200
Experimental info
	Sequence conflict	21	1	L → P in BAD96594. Ref.6
	Sequence conflict	119	1	F → S in BAD96594. Ref.6
	Sequence conflict	179	1	I → M in BAD96594. Ref.6
	Sequence conflict	205	1	P → S in BAD96594. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8C305DE30EFD4F82	FASTA	331	37,424
	10 20 30 40 50 60 MRPALAVGLV FAGCCSNVIF LELLARKHPG CGNIVTFAQF LFIAVEGFLF EADLGRKPPA 70 80 90 100 110 120 IPIRYYAIMV TMFFTVSVVN NYALNLNIAM PLHMIFRSGS LIANMILGII ILKKRYSIFK 130 140 150 160 170 180 YTSIALVSVG IFICTFMSAK QVTSQSSLSE NDGFQAFVWW LLGIGALTFA LLMSARMGIF 190 200 210 220 230 240 QETLYKRFGK HSKEALFYNH ALPLPGFVFL ASDIYDHAVL FNKSELYEIP VIGVTLPIMW 250 260 270 280 290 300 FYLLMNIITQ YVCIRGVFIL TTECASLTVT LVVTLRKFVS LIFSILYFQN PFTLWHWLGT 310 320 330 LFVFIGTLMY TEVWNNLGTT KSEPQKDSKK N
	Isoform 2 [UniParc]. Checksum: 9FB3C66ACF052788	FASTA	231	25,739
	10 20 30 40 50 60 MRPALAVGLV FAGCCSNVIF LELLARKHPG CGNIVTFAQF LFIAVEGFLF EADLGRKPPA 70 80 90 100 110 120 IPIRYYAIMV TMFFTVSVVN NYALNLNIAM PLHMIFRSGS LIANMILGII ILKKRYSIFK 130 140 150 160 170 180 YTSIALVSVG IFICTFMSAK QVTSQSSLSE NDGFQAFVWW LLGIGALTFA LLMSARMGIF 190 200 210 220 230 QETLYKRFGK HSKEALFYNH ALPLPGFVFL ASDIYDHAVL FNKSGEFGVR Q
	Isoform 3 [UniParc]. Checksum: B58AEBAECC3CE6FA	FASTA	253	28,357
	10 20 30 40 50 60 MRPALAVGLV FAGCCSNVIF LELLARKHPG CGNIVTFAQF LFIAVEGFLF EADLGRKPPA 70 80 90 100 110 120 IPIRYYAIMV TMFFTVSVVN NYALNLNIAM PLHMIFRSGS LIANMILGII ILKKRYSIFK 130 140 150 160 170 180 YTSIALVSVG IFICTFMSAK QVTSQSSLSE NDGFQAFVWW LLGIGALTFA LLMSARMGIF 190 200 210 220 230 240 QETLYKRFGK HSKEALFYNH ALPLPGFVFL ASDIYDHAVL FNKSELYEIP VIGVTLPIMW 250 FYLLMNIITQ TPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human solute carrier gene SLC35B4 encodes a bifunctional nucleotide sugar transporter with specificity for UDP-xylose and UDP-N-acetylglucosamine." Ashikov A., Routier F., Fuhlrott J., Helmus Y., Wild M., Gerardy-Schahn R., Bakker H. J. Biol. Chem. 280:27230-27235(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION.
[2]	"Molecular cloning of full-length cDNA similar to Yeast Yea4p from human liver cDNA library." Moriya Y., Ito K., Aburatani H., Suzuki H., Sugiyama Y. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Liver.
[3]	"Molecular cloning and characterisation of human yea4p." Kobayashi T., Burchell B. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-331 (ISOFORM 3).
[5]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[6]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[7]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ971941 mRNA. Translation: CAI98963.1. AB052892 mRNA. Translation: BAB61040.1. AJ315497 mRNA. Translation: CAC84567.1. AJ315498 mRNA. Translation: CAC84568.1. AK027603 mRNA. Translation: BAB55225.1. AK027726 mRNA. Translation: BAB55325.1. Different initiation. AK075368 mRNA. Translation: BAC11573.1. AK222874 mRNA. Translation: BAD96594.1. AC008154 Genomic DNA. No translation available. CH236950 Genomic DNA. Translation: EAL24071.1. CH471070 Genomic DNA. Translation: EAW83810.1. BC008413 mRNA. Translation: AAH08413.1.
IPI	IPI00299921. IPI00655802. IPI00655974.
RefSeq	NP_116215.1. NM_032826.4.
UniGene	Hs.490181.
3D structure databases
ProteinModelPortal	Q969S0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000367770.
Protein family/group databases
TCDB	2.A.7.10.2. drug/metabolite transporter (DMT) superfamily.
Polymorphism databases
DMDM	74751727.
Proteomic databases
PaxDb	Q969S0.
PRIDE	Q969S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378509; ENSP00000367770; ENSG00000205060.
GeneID	84912.
KEGG	hsa:84912.
UCSC	uc003vrn.3. human. uc003vro.4. human.
Organism-specific databases
CTD	84912.
GeneCards	GC07M133974.
HGNC	HGNC:20584. SLC35B4.
HPA	HPA049779.
MIM	610923. gene.
neXtProt	NX_Q969S0.
PharmGKB	PA134922110.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250393.
HOGENOM	HOG000234632.
HOVERGEN	HBG093926.
InParanoid	Q969S0.
KO	K15278.
OMA	LLYTEVW.
OrthoDB	EOG447FTJ.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q969S0.
Bgee	Q969S0.
CleanEx	HS_SLC35B4.
Genevestigator	Q969S0.
GermOnline	ENSG00000205060. Homo sapiens.
Family and domain databases
InterPro	IPR013657. UAA. [Graphical view]
Pfam	PF08449. UAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC35B4. human.
GenomeRNAi	84912.
NextBio	75300.
SOURCE	Search...

Entry information

Entry name

S35B4_HUMAN

Accession

Primary (citable) accession number: Q969S0
Secondary accession number(s): A4D1P3 , A6NNS4, Q53GQ7, Q8TCU7, Q96K33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.