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Q969R5 (LMBL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lethal(3)malignant brain tumor-like protein 2

Short name=H-l(3)mbt-like protein 2
Short name=L(3)mbt-like protein 2
Gene names
Name:L3MBTL2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. Ref.12

Subunit structure

Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, BAT8 and YAF2.

Subcellular location

Nucleus Probable.

Sequence similarities

Contains 1 FCS-type zinc finger.

Contains 4 MBT repeats.

Sequence caution

The sequence BAB84917.1 differs from that shown. Reason: Intron retention.

The sequence BAC04936.1 differs from that shown. Reason: Intron retention.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HDAC3O153796EBI-739909,EBI-607682

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q969R5-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q969R5-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     608-614: EPATPLK → GKLPRSL
     615-705: Missing.
Isoform 3 (identifier: Q969R5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     608-617: EPATPLKAKE → GVGSRGPKRL
     618-705: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Lethal(3)malignant brain tumor-like protein 2
PRO_0000084448

Regions

Repeat179 – 283105MBT 1
Repeat291 – 391101MBT 2
Repeat397 – 500104MBT 3
Repeat508 – 60497MBT 4
Zinc finger81 – 11636FCS-type
Compositional bias17 – 204Poly-Glu
Compositional bias620 – 6245Poly-Lys

Amino acid modifications

Modified residue131Phosphoserine Ref.8
Modified residue3381Phosphoserine Ref.11
Modified residue6831Phosphoserine Ref.8
Modified residue6881Phosphoserine Ref.8
Modified residue6891Phosphoserine Ref.8

Natural variations

Alternative sequence608 – 61710EPATPLKAKE → GVGSRGPKRL in isoform 3.
VSP_003906
Alternative sequence608 – 6147EPATPLK → GKLPRSL in isoform 2.
VSP_003904
Alternative sequence615 – 70591Missing in isoform 2.
VSP_003905
Alternative sequence618 – 70588Missing in isoform 3.
VSP_003907
Natural variant71I → V.
Corresponds to variant rs3804097 [ dbSNP | Ensembl ].
VAR_033998
Natural variant3001R → W.
Corresponds to variant rs2277846 [ dbSNP | Ensembl ].
VAR_015093
Natural variant3371V → A.
Corresponds to variant rs34289721 [ dbSNP | Ensembl ].
VAR_061675

Secondary structure

.................................................................................... 705
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8FC86A440982FFA7

FASTA70579,110
        10         20         30         40         50         60 
MEKPRSIEET PSSEPMEEEE DDDLELFGGY DSFRSYNSSV GSESSSYLEE SSEAENEDRE 

        70         80         90        100        110        120 
AGELPTSPLH LLSPGTPRSL DGSGSEPAVC EMCGIVGTRE AFFSKTKRFC SVSCSRSYSS 

       130        140        150        160        170        180 
NSKKASILAR LQGKPPTKKA KVLHKAAWSA KIGAFLHSQG TGQLADGTPT GQDALVLGFD 

       190        200        210        220        230        240 
WGKFLKDHSY KAAPVSCFKH VPLYDQWEDV MKGMKVEVLN SDAVLPSRVY WIASVIQTAG 

       250        260        270        280        290        300 
YRVLLRYEGF ENDASHDFWC NLGTVDVHPI GWCAINSKIL VPPRTIHAKF TDWKGYLMKR 

       310        320        330        340        350        360 
LVGSRTLPVD FHIKMVESMK YPFRQGMRLE VVDKSQVSRT RMAVVDTVIG GRLRLLYEDG 

       370        380        390        400        410        420 
DSDDDFWCHM WSPLIHPVGW SRRVGHGIKM SERRSDMAHH PTFRKIYCDA VPYLFKKVRA 

       430        440        450        460        470        480 
VYTEGGWFEE GMKLEAIDPL NLGNICVATV CKVLLDGYLM ICVDGGPSTD GLDWFCYHAS 

       490        500        510        520        530        540 
SHAIFPATFC QKNDIELTPP KGYEAQTFNW ENYLEKTKSK AAPSRLFNMD CPNHGFKVGM 

       550        560        570        580        590        600 
KLEAVDLMEP RLICVATVKR VVHRLLSIHF DGWDSEYDQW VDCESPDIYP VGWCELTGYQ 

       610        620        630        640        650        660 
LQPPVAAEPA TPLKAKEATK KKKKQFGKKR KRIPPTKTRP LRQGSKKPLL EDDPQGARKI 

       670        680        690        700 
SSEPVPGEII AVRVKEEHLD VASPDKASSP ELPVSVENIK QETDD 

« Hide

Isoform 2 (B) [UniParc].

Checksum: 6098B154D7387790
Show »

FASTA61469,008
Isoform 3 [UniParc].

Checksum: 2AD8D020C5715608
Show »

FASTA61769,264

References

« Hide 'large scale' references
[1]"Molecular characterization of h-l(3)mbt-like: a new member of the human mbt family."
Wismar J.
FEBS Lett. 507:119-121(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Amygdala.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Small intestine and Spleen.
[4]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[7]"A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells."
Ogawa H., Ishiguro K., Gaubatz S., Livingston D.M., Nakatani Y.
Science 296:1132-1136(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF COMPLEX WITH E2F6; TFDP1; MAX; MGA; EUHMTASE1; CBX3; RING1; RNF2; MBLR; BAT8 AND YAF2.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-683; SER-688 AND SER-689, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Methylation-state-specific recognition of histones by the MBT repeat protein L3MBTL2."
Guo Y., Nady N., Qi C., Allali-Hassani A., Zhu H., Pan P., Adams-Cioaba M.A., Amaya M.F., Dong A., Vedadi M., Schapira M., Read R.J., Arrowsmith C.H., Min J.
Nucleic Acids Res. 37:2204-2210(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 170-625 IN COMPLEX WITH MONOMETHYLATED HISTONE H4 PEPTIDE, FUNCTION.
[13]"Solution structure of the FCS zinc finger domain of the human polycomb group protein L(3)mbt-like 2."
Lechtenberg B.C., Allen M.D., Rutherford T.J., Freund S.M., Bycroft M.
Protein Sci. 18:657-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 82-124 IN COMPLEX WITH ZINC IONS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ305226 mRNA. Translation: CAC37794.1.
AJ305227 mRNA. Translation: CAC37795.1.
AL136564 mRNA. Translation: CAB66499.2.
AK074091 mRNA. Translation: BAB84917.1. Sequence problems.
AK097052 mRNA. Translation: BAC04936.1. Sequence problems.
CR456482 mRNA. Translation: CAG30368.1.
AL035658, AL035681 Genomic DNA. Translation: CAI23036.1.
AL035681, AL035658 Genomic DNA. Translation: CAI22654.1.
BC017191 mRNA. Translation: AAH17191.1.
RefSeqNP_113676.2. NM_031488.4.
UniGeneHs.517641.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2W0TNMR-A82-124[»]
3CEYX-ray2.20A/B170-625[»]
3F70X-ray2.10A/B170-625[»]
ProteinModelPortalQ969R5.
SMRQ969R5. Positions 82-124, 143-626.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123753. 18 interactions.
IntActQ969R5. 6 interactions.
MINTMINT-1483852.
STRING9606.ENSP00000216237.

PTM databases

PhosphoSiteQ969R5.

Polymorphism databases

DMDM27734418.

Proteomic databases

PaxDbQ969R5.
PRIDEQ969R5.

Protocols and materials databases

DNASU83746.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216237; ENSP00000216237; ENSG00000100395. [Q969R5-1]
ENST00000452106; ENSP00000414423; ENSG00000100395. [Q969R5-2]
GeneID83746.
KEGGhsa:83746.
UCSCuc003azo.3. human. [Q969R5-1]
uc010gyi.1. human. [Q969R5-3]

Organism-specific databases

CTD83746.
GeneCardsGC22P041601.
HGNCHGNC:18594. L3MBTL2.
HPAHPA000815.
MIM611865. gene.
neXtProtNX_Q969R5.
PharmGKBPA38356.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG271713.
HOGENOMHOG000231220.
HOVERGENHBG057974.
InParanoidQ969R5.
OMADGWDNEY.
OrthoDBEOG7M98G1.
PhylomeDBQ969R5.
TreeFamTF316498.

Gene expression databases

ArrayExpressQ969R5.
BgeeQ969R5.
CleanExHS_L3MBTL2.
GenevestigatorQ969R5.

Family and domain databases

InterProIPR004092. Mbt.
IPR012313. Znf_FCS.
[Graphical view]
PfamPF02820. MBT. 4 hits.
[Graphical view]
SMARTSM00561. MBT. 4 hits.
[Graphical view]
PROSITEPS51079. MBT. 4 hits.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSL3MBTL2. human.
EvolutionaryTraceQ969R5.
GeneWikiL3MBTL2.
GenomeRNAi83746.
NextBio72771.
PROQ969R5.
SOURCESearch...

Entry information

Entry nameLMBL2_HUMAN
AccessionPrimary (citable) accession number: Q969R5
Secondary accession number(s): Q8TEN1 expand/collapse secondary AC list , Q96SC4, Q9BQI2, Q9UGS4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM