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Protein

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma

Gene

PPP2R3C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate MCM3AP phosphorylation through phosphatase recruitment. May act as a negative regulator of ABCB1 expression and function through the dephosphorylation of ABCB1 by TFPI2/PPP2R3C complex. May play a role in the activation-induced cell death of B-cells.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi286 – 297121Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. activation of protein kinase activity Source: Ensembl
  2. B cell homeostasis Source: GO_Central
  3. cortical cytoskeleton organization Source: GO_Central
  4. microtubule cytoskeleton organization Source: GO_Central
  5. positive regulation of B cell differentiation Source: GO_Central
  6. regulation of antimicrobial humoral response Source: Ensembl
  7. regulation of mitochondrial depolarization Source: Ensembl
  8. spleen development Source: Ensembl
  9. T cell homeostasis Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma
Alternative name(s):
Protein phosphatase subunit G5PR
Rhabdomyosarcoma antigen MU-RMS-40.6A/6C
Gene namesi
Name:PPP2R3C
Synonyms:C14orf10, G5PR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:17485. PPP2R3C.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Excluded from the nucleoli. Localization is cell cycle-dependent. Localizes to the cytoplasm during cytokinesis.1 Publication

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. spindle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162400008.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gammaPRO_0000277833Add
BLAST

Proteomic databases

MaxQBiQ969Q6.
PaxDbiQ969Q6.
PRIDEiQ969Q6.

PTM databases

PhosphoSiteiQ969Q6.

Expressioni

Tissue specificityi

Ubiquitously expressed in brain and other tissues.2 Publications

Developmental stagei

Expressed in fetal brain.1 Publication

Gene expression databases

BgeeiQ969Q6.
CleanExiHS_PPP2R3C.
ExpressionAtlasiQ969Q6. baseline and differential.
GenevestigatoriQ969Q6.

Organism-specific databases

HPAiHPA040835.

Interactioni

Subunit structurei

Interacts with MCM3AP/GANP. Interacts with PPP5C, and the phosphatase 2A core enzyme composed of the PPP2CA catalytic subunit and the constant regulatory subunit PPP2R1A. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity2 Publications

Protein-protein interaction databases

BioGridi120344. 10 interactions.
IntActiQ969Q6. 4 interactions.
STRINGi9606.ENSP00000261475.

Structurei

3D structure databases

ProteinModelPortaliQ969Q6.
SMRiQ969Q6. Positions 188-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini273 – 30836EF-hand 1Add
BLAST
Domaini341 – 37636EF-hand 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi226 – 2294Poly-Phe

Sequence similaritiesi

Contains 2 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG279493.
GeneTreeiENSGT00530000063265.
HOVERGENiHBG055242.
KOiK11583.
OMAiQESNWFS.
PhylomeDBiQ969Q6.
TreeFamiTF318412.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969Q6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDWKEVLRRR LATPNTCPNK KKSEQELKDE EMDLFTKYYS EWKGGRKNTN
60 70 80 90 100
EFYKTIPRFY YRLPAEDEVL LQKLREESRA VFLQRKSREL LDNEELQNLW
110 120 130 140 150
FLLDKHQTPP MIGEEAMINY ENFLKVGEKA GAKCKQFFTA KVFAKLLHTD
160 170 180 190 200
SYGRISIMQF FNYVMRKVWL HQTRIGLSLY DVAGQGYLRE SDLENYILEL
210 220 230 240 250
IPTLPQLDGL EKSFYSFYVC TAVRKFFFFL DPLRTGKIKI QDILACSFLD
260 270 280 290 300
DLLELRDEEL SKESQETNWF SAPSALRVYG QYLNLDKDHN GMLSKEELSR
310 320 330 340 350
YGTATMTNVF LDRVFQECLT YDGEMDYKTY LDFVLALENR KEPAALQYIF
360 370 380 390 400
KLLDIENKGY LNVFSLNYFF RAIQELMKIH GQDPVSFQDV KDEIFDMVKP
410 420 430 440 450
KDPLKISLQD LINSNQGDTV TTILIDLNGF WTYENREALV ANDSENSADL

DDT
Length:453
Mass (Da):53,316
Last modified:December 1, 2001 - v1
Checksum:i9749B7188B2F7599
GO
Isoform 2 (identifier: Q969Q6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.

Show »
Length:343
Mass (Da):39,806
Checksum:iA921EE6858995288
GO

Sequence cautioni

The sequence AAT44532.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAT44533.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061H → R in BAA91308 (PubMed:14702039).Curated
Sequence conflicti240 – 2401I → T in AAT44532 (Ref. 6) Curated
Sequence conflicti301 – 3011Y → H in BAA91308 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 110110Missing in isoform 2. 2 PublicationsVSP_023113Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157304 mRNA. Translation: AAO17045.1.
AK000651 mRNA. Translation: BAA91308.1.
AK293717 mRNA. Translation: BAG57148.1.
CH471078 Genomic DNA. Translation: EAW65886.1.
CH471078 Genomic DNA. Translation: EAW65887.1.
CH471078 Genomic DNA. Translation: EAW65889.1.
CH471078 Genomic DNA. Translation: EAW65890.1.
BC006823 mRNA. Translation: AAH06823.1.
BC010293 mRNA. Translation: AAH10293.1.
BC012563 mRNA. Translation: AAH12563.1.
BC063438 mRNA. Translation: AAH63438.1.
BX248043 mRNA. Translation: CAD62352.1.
AY518535 mRNA. Translation: AAT44532.1. Different initiation.
AY518537 mRNA. Translation: AAT44533.1. Different initiation.
CCDSiCCDS9654.1. [Q969Q6-1]
RefSeqiNP_060387.2. NM_017917.2. [Q969Q6-1]
XP_005267839.1. XM_005267782.1. [Q969Q6-1]
XP_005267841.1. XM_005267784.1. [Q969Q6-2]
UniGeneiHs.530712.
Hs.621667.

Genome annotation databases

EnsembliENST00000261475; ENSP00000261475; ENSG00000092020. [Q969Q6-1]
GeneIDi55012.
KEGGihsa:55012.
UCSCiuc001wss.3. human. [Q969Q6-1]

Polymorphism databases

DMDMi74762643.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157304 mRNA. Translation: AAO17045.1.
AK000651 mRNA. Translation: BAA91308.1.
AK293717 mRNA. Translation: BAG57148.1.
CH471078 Genomic DNA. Translation: EAW65886.1.
CH471078 Genomic DNA. Translation: EAW65887.1.
CH471078 Genomic DNA. Translation: EAW65889.1.
CH471078 Genomic DNA. Translation: EAW65890.1.
BC006823 mRNA. Translation: AAH06823.1.
BC010293 mRNA. Translation: AAH10293.1.
BC012563 mRNA. Translation: AAH12563.1.
BC063438 mRNA. Translation: AAH63438.1.
BX248043 mRNA. Translation: CAD62352.1.
AY518535 mRNA. Translation: AAT44532.1. Different initiation.
AY518537 mRNA. Translation: AAT44533.1. Different initiation.
CCDSiCCDS9654.1. [Q969Q6-1]
RefSeqiNP_060387.2. NM_017917.2. [Q969Q6-1]
XP_005267839.1. XM_005267782.1. [Q969Q6-1]
XP_005267841.1. XM_005267784.1. [Q969Q6-2]
UniGeneiHs.530712.
Hs.621667.

3D structure databases

ProteinModelPortaliQ969Q6.
SMRiQ969Q6. Positions 188-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120344. 10 interactions.
IntActiQ969Q6. 4 interactions.
STRINGi9606.ENSP00000261475.

PTM databases

PhosphoSiteiQ969Q6.

Polymorphism databases

DMDMi74762643.

Proteomic databases

MaxQBiQ969Q6.
PaxDbiQ969Q6.
PRIDEiQ969Q6.

Protocols and materials databases

DNASUi55012.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261475; ENSP00000261475; ENSG00000092020. [Q969Q6-1]
GeneIDi55012.
KEGGihsa:55012.
UCSCiuc001wss.3. human. [Q969Q6-1]

Organism-specific databases

CTDi55012.
GeneCardsiGC14M035554.
H-InvDBHIX0026726.
HGNCiHGNC:17485. PPP2R3C.
HPAiHPA040835.
MIMi615902. gene.
neXtProtiNX_Q969Q6.
PharmGKBiPA162400008.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG279493.
GeneTreeiENSGT00530000063265.
HOVERGENiHBG055242.
KOiK11583.
OMAiQESNWFS.
PhylomeDBiQ969Q6.
TreeFamiTF318412.

Miscellaneous databases

GeneWikiiPPP2R3C.
GenomeRNAii55012.
NextBioi58369.
PROiQ969Q6.
SOURCEiSearch...

Gene expression databases

BgeeiQ969Q6.
CleanExiHS_PPP2R3C.
ExpressionAtlasiQ969Q6. baseline and differential.
GenevestigatoriQ969Q6.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Defining a holoprosencephaly locus on human chromosome 14q13 and characterization of potential candidate genes."
    Kamnasaran D., Chen C.-P., Devriendt K., Mehta L., Cox D.W.
    Genomics 85:608-621(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Gastric carcinoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Bone marrow, Brain and Placenta.
  5. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-167 (ISOFORM 1).
    Tissue: Placenta.
  6. "SEREX-defined rhabdomyosarcoma antigens."
    Behrends U., Gotz C., Mautner J.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-453 (ISOFORM 1).
    Tissue: Embryonic rhabdomyosarcoma.
  7. "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR."
    Kono Y., Maeda K., Kuwahara K., Yamamoto H., Miyamoto E., Yonezawa K., Takagi K., Sakaguchi N.
    Genes Cells 7:821-834(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP2CA; PPP2R1A AND PPP5C, TISSUE SPECIFICITY.
  8. "Protein phosphatase complex PP5/PPP2R3C dephosphorylates P-glycoprotein/ABCB1 and down-regulates the expression and function."
    Katayama K., Yamaguchi M., Noguchi K., Sugimoto Y.
    Cancer Lett. 345:124-131(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFPI2 AND ABCB1, FUNCTION.

Entry informationi

Entry nameiP2R3C_HUMAN
AccessioniPrimary (citable) accession number: Q969Q6
Secondary accession number(s): B4DEN7
, D3DS97, D3DS98, Q5GJ55, Q5GJ56, Q6P4G2, Q86TZ3, Q9NWR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.