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Q969P0 (IGSF8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Immunoglobulin superfamily member 8

Short name=IgSF8
Alternative name(s):
CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
Short name=EWI-2
Keratinocytes-associated transmembrane protein 4
Short name=KCT-4
LIR-D1
Prostaglandin regulatory-like protein
Short name=PGRL
CD_antigen=CD316
Gene names
Name:IGSF8
Synonyms:CD81P3, EWI2, KCT4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length613 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. Ref.1 Ref.3 Ref.8 Ref.9 Ref.10

Subunit structure

Interacts directly with CD82, CD81/tetraspanin-28 and CD9/tetraspanin-29. Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1. Ref.1 Ref.8 Ref.9 Ref.10

Subcellular location

Cell membrane By similarity; Single-pass membrane protein.

Tissue specificity

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets. Ref.1 Ref.2 Ref.8

Domain

The Ig-like C2-type domains 3 and 4 are required for interaction with CD81 By similarity.

The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization By similarity.

Sequence similarities

Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q969P0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q969P0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-315: ELRLGKEGTD...AVTVGPGERR → MRGRSWHWAA...GSFVWARKGP
Note: No experimental confirmation available.
Isoform 3 (identifier: Q969P0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     475-561: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.7
Chain28 – 613586Immunoglobulin superfamily member 8
PRO_0000226247

Regions

Topological domain28 – 579552Extracellular Potential
Transmembrane580 – 60021Helical; Potential
Topological domain601 – 61313Cytoplasmic Potential
Domain28 – 149122Ig-like C2-type 1
Domain162 – 286125Ig-like C2-type 2
Domain303 – 424122Ig-like C2-type 3
Domain431 – 560130Ig-like C2-type 4
Motif274 – 2763EWI motif

Amino acid modifications

Lipidation6041S-palmitoyl cysteine By similarity
Lipidation6051S-palmitoyl cysteine By similarity
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation3271N-linked (GlcNAc...) Ref.11
Glycosylation4631N-linked (GlcNAc...) Ref.11
Disulfide bond49 ↔ 127 By similarity
Disulfide bond186 ↔ 270 By similarity
Disulfide bond326 ↔ 406 By similarity
Disulfide bond462 ↔ 544 By similarity

Natural variations

Alternative sequence1 – 241241Missing in isoform 2.
VSP_017429
Alternative sequence242 – 31574ELRLG…PGERR → MRGRSWHWAAWRGQAHRSTH TWQCPLGDLCPRHQLGGQLC RKWWESGQTWPWRLELPMLS DWLQGSFVWARKGP in isoform 2.
VSP_017430
Alternative sequence475 – 56187Missing in isoform 3.
VSP_017431

Experimental info

Sequence conflict3031S → R in AAM94901. Ref.4
Sequence conflict3061A → P in AAM94901. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: A7255D54D42EFB88

FASTA61365,034
        10         20         30         40         50         60 
MGALRPTLLP PSLPLLLLLM LGMGCWAREV LVPEGPLYRV AGTAVSISCN VTGYEGPAQQ 

        70         80         90        100        110        120 
NFEWFLYRPE APDTALGIVS TKDTQFSYAV FKSRVVAGEV QVQRLQGDAV VLKIARLQAQ 

       130        140        150        160        170        180 
DAGIYECHTP STDTRYLGSY SGKVELRVLP DVLQVSAAPP GPRGRQAPTS PPRMTVHEGQ 

       190        200        210        220        230        240 
ELALGCLART STQKHTHLAV SFGRSVPEAP VGRSTLQEVV GIRSDLAVEA GAPYAERLAA 

       250        260        270        280        290        300 
GELRLGKEGT DRYRMVVGGA QAGDAGTYHC TAAEWIQDPD GSWAQIAEKR AVLAHVDVQT 

       310        320        330        340        350        360 
LSSQLAVTVG PGERRIGPGE PLELLCNVSG ALPPAGRHAA YSVGWEMAPA GAPGPGRLVA 

       370        380        390        400        410        420 
QLDTEGVGSL GPGYEGRHIA MEKVASRTYR LRLEAARPGD AGTYRCLAKA YVRGSGTRLR 

       430        440        450        460        470        480 
EAASARSRPL PVHVREEGVV LEAVAWLAGG TVYRGETASL LCNISVRGGP PGLRLAASWW 

       490        500        510        520        530        540 
VERPEDGELS SVPAQLVGGV GQDGVAELGV RPGGGPVSVE LVGPRSHRLR LHSLGPEDEG 

       550        560        570        580        590        600 
VYHCAPSAWV QHADYSWYQA GSARSGPVTV YPYMHALDTL FVPLLVGTGV ALVTGATVLG 

       610 
TITCCFMKRL RKR 

« Hide

Isoform 2 [UniParc].

Checksum: 4872C8D6CC303638
Show »

FASTA37240,191
Isoform 3 [UniParc].

Checksum: C2A88D773A4F4802
Show »

FASTA52655,808

References

« Hide 'large scale' references
[1]"EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
Stipp C.S., Kolesnikova T.V., Hemler M.E.
J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CD81 AND CD9.
[2]"EWI-2 is a new component of the tetraspanin web in hepatocytes and lymphoid cells."
Charrin S., Le Naour F., Labas V., Billard M., Le Caer J.-P., Emile J.-F., Petit M.-A., Boucheix C., Rubinstein E.
Biochem. J. 373:409-421(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
[3]"Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes."
Bonkobara M., Das A., Takao J., Cruz P.D. Jr., Ariizumi K.
Br. J. Dermatol. 148:654-664(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[4]"Identification of novel membrane proteins."
Zhang W., Li N., Wan T., Cao X.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Kidney.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph.
[7]"Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini."
Xu G., Shin S.B., Jaffrey S.R.
Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 28-40.
Tissue: Leukemic T-cell.
[8]"EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CD82, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
[9]"EWI-2 regulates alpha3beta1 integrin-dependent cell functions on laminin-5."
Stipp C.S., Kolesnikova T.V., Hemler M.E.
J. Cell Biol. 163:1167-1177(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH INTEGRIN ALPHA-3 BETA-1.
[10]"EWI-2 modulates lymphocyte integrin alpha4beta1 functions."
Kolesnikova T.V., Stipp C.S., Rao R.M., Lane W.S., Luscinskas F.W., Hemler M.E.
Blood 103:3013-3019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH INTEGRIN ALPHA-4 BETA-1.
[11]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-327 AND ASN-463.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF407274 mRNA. Translation: AAL01052.1.
AY044845 mRNA. Translation: AAK92220.1.
AY157579 mRNA. Translation: AAO13163.1.
AF311906 mRNA. Translation: AAM94901.1.
AK055843 mRNA. Translation: BAB71027.1.
BC004108 mRNA. Translation: AAH04108.2.
BC053881 mRNA. Translation: AAH53881.1.
CCDSCCDS1195.1. [Q969P0-1]
RefSeqNP_001193594.1. NM_001206665.2. [Q969P0-1]
NP_443100.1. NM_052868.4. [Q969P0-1]
XP_005245671.1. XM_005245614.1. [Q969P0-1]
UniGeneHs.332012.

3D structure databases

ProteinModelPortalQ969P0.
SMRQ969P0. Positions 29-65, 108-134.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125011. 164 interactions.
IntActQ969P0. 1 interaction.
MINTMINT-4536413.
STRING9606.ENSP00000316664.

PTM databases

PhosphoSiteQ969P0.

Polymorphism databases

DMDM74762642.

2D gel databases

UCD-2DPAGEQ969P0.

Proteomic databases

MaxQBQ969P0.
PaxDbQ969P0.
PRIDEQ969P0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000314485; ENSP00000316664; ENSG00000162729. [Q969P0-1]
ENST00000368086; ENSP00000357065; ENSG00000162729. [Q969P0-1]
GeneID93185.
KEGGhsa:93185.
UCSCuc001fuz.3. human. [Q969P0-1]

Organism-specific databases

CTD93185.
GeneCardsGC01M160061.
HGNCHGNC:17813. IGSF8.
HPAHPA011917.
MIM606644. gene.
neXtProtNX_Q969P0.
PharmGKBPA29767.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG28691.
HOGENOMHOG000113020.
HOVERGENHBG073047.
InParanoidQ969P0.
KOK06730.
OMAVTCCFMK.
OrthoDBEOG7H791Z.
PhylomeDBQ969P0.
TreeFamTF332702.

Gene expression databases

ArrayExpressQ969P0.
BgeeQ969P0.
CleanExHS_IGSF8.
GenevestigatorQ969P0.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 4 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIGSF8. human.
GeneWikiIGSF8.
GenomeRNAi93185.
NextBio78023.
PROQ969P0.
SOURCESearch...

Entry information

Entry nameIGSF8_HUMAN
AccessionPrimary (citable) accession number: Q969P0
Secondary accession number(s): Q8NG09, Q96DP4, Q9BTG9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries