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Protein

Immunoglobulin superfamily member 8

Gene

IGSF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.5 Publications

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • movement of cell or subcellular component Source: UniProtKB
  • nervous system development Source: UniProtKB
  • single fertilization Source: UniProtKB
  • skeletal muscle tissue development Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 8
Short name:
IgSF8
Alternative name(s):
CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
Short name:
EWI-2
Keratinocytes-associated transmembrane protein 4
Short name:
KCT-4
LIR-D1
Prostaglandin regulatory-like protein
Short name:
PGRL
CD_antigen: CD316
Gene namesi
Name:IGSF8
Synonyms:CD81P3, EWI2, KCT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:17813. IGSF8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 579ExtracellularSequence analysisAdd BLAST552
Transmembranei580 – 600HelicalSequence analysisAdd BLAST21
Topological domaini601 – 613CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi93185.
OpenTargetsiENSG00000162729.
PharmGKBiPA29767.

Polymorphism and mutation databases

BioMutaiIGSF8.
DMDMi74762642.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000022624728 – 613Immunoglobulin superfamily member 8Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 127PROSITE-ProRule annotation
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi186 ↔ 270PROSITE-ProRule annotation
Disulfide bondi326 ↔ 406PROSITE-ProRule annotation
Glycosylationi327N-linked (GlcNAc...)1 Publication1
Disulfide bondi462 ↔ 544PROSITE-ProRule annotation
Glycosylationi463N-linked (GlcNAc...)1 Publication1
Modified residuei518PhosphoserineCombined sources1
Lipidationi604S-palmitoyl cysteineBy similarity1
Lipidationi605S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ969P0.
MaxQBiQ969P0.
PaxDbiQ969P0.
PeptideAtlasiQ969P0.
PRIDEiQ969P0.

2D gel databases

UCD-2DPAGEQ969P0.

PTM databases

iPTMnetiQ969P0.
PhosphoSitePlusiQ969P0.
SwissPalmiQ969P0.

Expressioni

Tissue specificityi

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.3 Publications

Gene expression databases

BgeeiENSG00000162729.
CleanExiHS_IGSF8.
ExpressionAtlasiQ969P0. baseline and differential.
GenevisibleiQ969P0. HS.

Organism-specific databases

HPAiHPA011917.

Interactioni

Subunit structurei

Interacts directly with CD82, CD81/tetraspanin-28 and CD9/tetraspanin-29. Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-4P603723EBI-8293590,EBI-10178153
KRTAP10-7P604093EBI-8293590,EBI-10172290
KRTAP10-8P604105EBI-8293590,EBI-10171774
KRTAP10-9P604113EBI-8293590,EBI-10172052

Protein-protein interaction databases

BioGridi125011. 192 interactors.
IntActiQ969P0. 8 interactors.
MINTiMINT-4536413.
STRINGi9606.ENSP00000316664.

Structurei

3D structure databases

ProteinModelPortaliQ969P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 149Ig-like C2-type 1Add BLAST122
Domaini162 – 286Ig-like C2-type 2Add BLAST125
Domaini303 – 424Ig-like C2-type 3Add BLAST122
Domaini431 – 560Ig-like C2-type 4Add BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 276EWI motif3

Domaini

The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.By similarity
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHZZ. Eukaryota.
ENOG410XSYU. LUCA.
GeneTreeiENSGT00390000010278.
HOGENOMiHOG000113020.
HOVERGENiHBG073047.
InParanoidiQ969P0.
KOiK06730.
OMAiVTCCFMK.
OrthoDBiEOG091G00OL.
PhylomeDBiQ969P0.
TreeFamiTF332702.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGALRPTLLP PSLPLLLLLM LGMGCWAREV LVPEGPLYRV AGTAVSISCN
60 70 80 90 100
VTGYEGPAQQ NFEWFLYRPE APDTALGIVS TKDTQFSYAV FKSRVVAGEV
110 120 130 140 150
QVQRLQGDAV VLKIARLQAQ DAGIYECHTP STDTRYLGSY SGKVELRVLP
160 170 180 190 200
DVLQVSAAPP GPRGRQAPTS PPRMTVHEGQ ELALGCLART STQKHTHLAV
210 220 230 240 250
SFGRSVPEAP VGRSTLQEVV GIRSDLAVEA GAPYAERLAA GELRLGKEGT
260 270 280 290 300
DRYRMVVGGA QAGDAGTYHC TAAEWIQDPD GSWAQIAEKR AVLAHVDVQT
310 320 330 340 350
LSSQLAVTVG PGERRIGPGE PLELLCNVSG ALPPAGRHAA YSVGWEMAPA
360 370 380 390 400
GAPGPGRLVA QLDTEGVGSL GPGYEGRHIA MEKVASRTYR LRLEAARPGD
410 420 430 440 450
AGTYRCLAKA YVRGSGTRLR EAASARSRPL PVHVREEGVV LEAVAWLAGG
460 470 480 490 500
TVYRGETASL LCNISVRGGP PGLRLAASWW VERPEDGELS SVPAQLVGGV
510 520 530 540 550
GQDGVAELGV RPGGGPVSVE LVGPRSHRLR LHSLGPEDEG VYHCAPSAWV
560 570 580 590 600
QHADYSWYQA GSARSGPVTV YPYMHALDTL FVPLLVGTGV ALVTGATVLG
610
TITCCFMKRL RKR
Length:613
Mass (Da):65,034
Last modified:December 1, 2001 - v1
Checksum:iA7255D54D42EFB88
GO
Isoform 2 (identifier: Q969P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-315: ELRLGKEGTD...AVTVGPGERR → MRGRSWHWAA...GSFVWARKGP

Note: No experimental confirmation available.
Show »
Length:372
Mass (Da):40,191
Checksum:i4872C8D6CC303638
GO
Isoform 3 (identifier: Q969P0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     475-561: Missing.

Note: No experimental confirmation available.
Show »
Length:526
Mass (Da):55,808
Checksum:iC2A88D773A4F4802
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303S → R in AAM94901 (Ref. 4) Curated1
Sequence conflicti306A → P in AAM94901 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0174291 – 241Missing in isoform 2. 1 PublicationAdd BLAST241
Alternative sequenceiVSP_017430242 – 315ELRLG…PGERR → MRGRSWHWAAWRGQAHRSTH TWQCPLGDLCPRHQLGGQLC RKWWESGQTWPWRLELPMLS DWLQGSFVWARKGP in isoform 2. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_017431475 – 561Missing in isoform 3. 1 PublicationAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407274 mRNA. Translation: AAL01052.1.
AY044845 mRNA. Translation: AAK92220.1.
AY157579 mRNA. Translation: AAO13163.1.
AF311906 mRNA. Translation: AAM94901.1.
AK055843 mRNA. Translation: BAB71027.1.
BC004108 mRNA. Translation: AAH04108.2.
BC053881 mRNA. Translation: AAH53881.1.
CCDSiCCDS1195.1. [Q969P0-1]
RefSeqiNP_001193594.1. NM_001206665.2. [Q969P0-1]
NP_001307176.1. NM_001320247.1. [Q969P0-1]
NP_443100.1. NM_052868.5. [Q969P0-1]
XP_016858324.1. XM_017002835.1. [Q969P0-1]
XP_016858325.1. XM_017002836.1. [Q969P0-1]
XP_016858326.1. XM_017002837.1. [Q969P0-1]
UniGeneiHs.332012.

Genome annotation databases

EnsembliENST00000314485; ENSP00000316664; ENSG00000162729. [Q969P0-1]
ENST00000368086; ENSP00000357065; ENSG00000162729. [Q969P0-1]
ENST00000614243; ENSP00000477565; ENSG00000162729. [Q969P0-1]
GeneIDi93185.
KEGGihsa:93185.
UCSCiuc001fuz.4. human. [Q969P0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407274 mRNA. Translation: AAL01052.1.
AY044845 mRNA. Translation: AAK92220.1.
AY157579 mRNA. Translation: AAO13163.1.
AF311906 mRNA. Translation: AAM94901.1.
AK055843 mRNA. Translation: BAB71027.1.
BC004108 mRNA. Translation: AAH04108.2.
BC053881 mRNA. Translation: AAH53881.1.
CCDSiCCDS1195.1. [Q969P0-1]
RefSeqiNP_001193594.1. NM_001206665.2. [Q969P0-1]
NP_001307176.1. NM_001320247.1. [Q969P0-1]
NP_443100.1. NM_052868.5. [Q969P0-1]
XP_016858324.1. XM_017002835.1. [Q969P0-1]
XP_016858325.1. XM_017002836.1. [Q969P0-1]
XP_016858326.1. XM_017002837.1. [Q969P0-1]
UniGeneiHs.332012.

3D structure databases

ProteinModelPortaliQ969P0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125011. 192 interactors.
IntActiQ969P0. 8 interactors.
MINTiMINT-4536413.
STRINGi9606.ENSP00000316664.

PTM databases

iPTMnetiQ969P0.
PhosphoSitePlusiQ969P0.
SwissPalmiQ969P0.

Polymorphism and mutation databases

BioMutaiIGSF8.
DMDMi74762642.

2D gel databases

UCD-2DPAGEQ969P0.

Proteomic databases

EPDiQ969P0.
MaxQBiQ969P0.
PaxDbiQ969P0.
PeptideAtlasiQ969P0.
PRIDEiQ969P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314485; ENSP00000316664; ENSG00000162729. [Q969P0-1]
ENST00000368086; ENSP00000357065; ENSG00000162729. [Q969P0-1]
ENST00000614243; ENSP00000477565; ENSG00000162729. [Q969P0-1]
GeneIDi93185.
KEGGihsa:93185.
UCSCiuc001fuz.4. human. [Q969P0-1]

Organism-specific databases

CTDi93185.
DisGeNETi93185.
GeneCardsiIGSF8.
HGNCiHGNC:17813. IGSF8.
HPAiHPA011917.
MIMi606644. gene.
neXtProtiNX_Q969P0.
OpenTargetsiENSG00000162729.
PharmGKBiPA29767.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHZZ. Eukaryota.
ENOG410XSYU. LUCA.
GeneTreeiENSGT00390000010278.
HOGENOMiHOG000113020.
HOVERGENiHBG073047.
InParanoidiQ969P0.
KOiK06730.
OMAiVTCCFMK.
OrthoDBiEOG091G00OL.
PhylomeDBiQ969P0.
TreeFamiTF332702.

Miscellaneous databases

ChiTaRSiIGSF8. human.
GeneWikiiIGSF8.
GenomeRNAii93185.
PROiQ969P0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162729.
CleanExiHS_IGSF8.
ExpressionAtlasiQ969P0. baseline and differential.
GenevisibleiQ969P0. HS.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIGSF8_HUMAN
AccessioniPrimary (citable) accession number: Q969P0
Secondary accession number(s): Q8NG09, Q96DP4, Q9BTG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.