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Protein

Immunoglobulin superfamily member 8

Gene

IGSF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.5 Publications

GO - Biological processi

  • cell motility Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • nervous system development Source: UniProtKB
  • single fertilization Source: UniProtKB
  • skeletal muscle tissue development Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 8
Short name:
IgSF8
Alternative name(s):
CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
Short name:
EWI-2
Keratinocytes-associated transmembrane protein 4
Short name:
KCT-4
LIR-D1
Prostaglandin regulatory-like protein
Short name:
PGRL
CD_antigen: CD316
Gene namesi
Name:IGSF8
Synonyms:CD81P3, EWI2, KCT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162729.13
HGNCiHGNC:17813 IGSF8
MIMi606644 gene
neXtProtiNX_Q969P0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 579ExtracellularSequence analysisAdd BLAST552
Transmembranei580 – 600HelicalSequence analysisAdd BLAST21
Topological domaini601 – 613CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi93185
OpenTargetsiENSG00000162729
PharmGKBiPA29767

Polymorphism and mutation databases

BioMutaiIGSF8
DMDMi74762642

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000022624728 – 613Immunoglobulin superfamily member 8Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 127PROSITE-ProRule annotation
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi186 ↔ 270PROSITE-ProRule annotation
Disulfide bondi326 ↔ 406PROSITE-ProRule annotation
Glycosylationi327N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi462 ↔ 544PROSITE-ProRule annotation
Glycosylationi463N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei518PhosphoserineCombined sources1
Lipidationi604S-palmitoyl cysteineBy similarity1
Lipidationi605S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ969P0
MaxQBiQ969P0
PaxDbiQ969P0
PeptideAtlasiQ969P0
PRIDEiQ969P0

2D gel databases

UCD-2DPAGEQ969P0

PTM databases

iPTMnetiQ969P0
PhosphoSitePlusiQ969P0
SwissPalmiQ969P0

Expressioni

Tissue specificityi

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.3 Publications

Gene expression databases

BgeeiENSG00000162729
CleanExiHS_IGSF8
ExpressionAtlasiQ969P0 baseline and differential
GenevisibleiQ969P0 HS

Organism-specific databases

HPAiHPA011917

Interactioni

Subunit structurei

Interacts directly with CD82, CD81/tetraspanin-28 and CD9/tetraspanin-29. Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi125011, 201 interactors
IntActiQ969P0, 9 interactors
MINTiQ969P0
STRINGi9606.ENSP00000316664

Structurei

3D structure databases

ProteinModelPortaliQ969P0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 149Ig-like C2-type 1Add BLAST122
Domaini162 – 286Ig-like C2-type 2Add BLAST125
Domaini303 – 424Ig-like C2-type 3Add BLAST122
Domaini431 – 560Ig-like C2-type 4Add BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 276EWI motif3

Domaini

The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.By similarity
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.By similarity

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHZZ Eukaryota
ENOG410XSYU LUCA
GeneTreeiENSGT00390000010278
HOGENOMiHOG000113020
HOVERGENiHBG073047
InParanoidiQ969P0
KOiK06730
OMAiNFEWFLY
OrthoDBiEOG091G00OL
PhylomeDBiQ969P0
TreeFamiTF332702

Family and domain databases

Gene3Di2.60.40.10, 4 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 4 hits
SUPFAMiSSF48726 SSF48726, 4 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q969P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGALRPTLLP PSLPLLLLLM LGMGCWAREV LVPEGPLYRV AGTAVSISCN
60 70 80 90 100
VTGYEGPAQQ NFEWFLYRPE APDTALGIVS TKDTQFSYAV FKSRVVAGEV
110 120 130 140 150
QVQRLQGDAV VLKIARLQAQ DAGIYECHTP STDTRYLGSY SGKVELRVLP
160 170 180 190 200
DVLQVSAAPP GPRGRQAPTS PPRMTVHEGQ ELALGCLART STQKHTHLAV
210 220 230 240 250
SFGRSVPEAP VGRSTLQEVV GIRSDLAVEA GAPYAERLAA GELRLGKEGT
260 270 280 290 300
DRYRMVVGGA QAGDAGTYHC TAAEWIQDPD GSWAQIAEKR AVLAHVDVQT
310 320 330 340 350
LSSQLAVTVG PGERRIGPGE PLELLCNVSG ALPPAGRHAA YSVGWEMAPA
360 370 380 390 400
GAPGPGRLVA QLDTEGVGSL GPGYEGRHIA MEKVASRTYR LRLEAARPGD
410 420 430 440 450
AGTYRCLAKA YVRGSGTRLR EAASARSRPL PVHVREEGVV LEAVAWLAGG
460 470 480 490 500
TVYRGETASL LCNISVRGGP PGLRLAASWW VERPEDGELS SVPAQLVGGV
510 520 530 540 550
GQDGVAELGV RPGGGPVSVE LVGPRSHRLR LHSLGPEDEG VYHCAPSAWV
560 570 580 590 600
QHADYSWYQA GSARSGPVTV YPYMHALDTL FVPLLVGTGV ALVTGATVLG
610
TITCCFMKRL RKR
Length:613
Mass (Da):65,034
Last modified:December 1, 2001 - v1
Checksum:iA7255D54D42EFB88
GO
Isoform 2 (identifier: Q969P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-315: ELRLGKEGTD...AVTVGPGERR → MRGRSWHWAA...GSFVWARKGP

Note: No experimental confirmation available.
Show »
Length:372
Mass (Da):40,191
Checksum:i4872C8D6CC303638
GO
Isoform 3 (identifier: Q969P0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     475-561: Missing.

Note: No experimental confirmation available.
Show »
Length:526
Mass (Da):55,808
Checksum:iC2A88D773A4F4802
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303S → R in AAM94901 (Ref. 4) Curated1
Sequence conflicti306A → P in AAM94901 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0174291 – 241Missing in isoform 2. 1 PublicationAdd BLAST241
Alternative sequenceiVSP_017430242 – 315ELRLG…PGERR → MRGRSWHWAAWRGQAHRSTH TWQCPLGDLCPRHQLGGQLC RKWWESGQTWPWRLELPMLS DWLQGSFVWARKGP in isoform 2. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_017431475 – 561Missing in isoform 3. 1 PublicationAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407274 mRNA Translation: AAL01052.1
AY044845 mRNA Translation: AAK92220.1
AY157579 mRNA Translation: AAO13163.1
AF311906 mRNA Translation: AAM94901.1
AK055843 mRNA Translation: BAB71027.1
BC004108 mRNA Translation: AAH04108.2
BC053881 mRNA Translation: AAH53881.1
CCDSiCCDS1195.1 [Q969P0-1]
RefSeqiNP_001193594.1, NM_001206665.2 [Q969P0-1]
NP_001307176.1, NM_001320247.1 [Q969P0-1]
NP_443100.1, NM_052868.5 [Q969P0-1]
XP_016858324.1, XM_017002835.1 [Q969P0-1]
XP_016858325.1, XM_017002836.1 [Q969P0-1]
XP_016858326.1, XM_017002837.1 [Q969P0-1]
UniGeneiHs.332012

Genome annotation databases

EnsembliENST00000314485; ENSP00000316664; ENSG00000162729 [Q969P0-1]
ENST00000368086; ENSP00000357065; ENSG00000162729 [Q969P0-1]
ENST00000614243; ENSP00000477565; ENSG00000162729 [Q969P0-1]
GeneIDi93185
KEGGihsa:93185
UCSCiuc001fuz.4 human [Q969P0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIGSF8_HUMAN
AccessioniPrimary (citable) accession number: Q969P0
Secondary accession number(s): Q8NG09, Q96DP4, Q9BTG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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