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Q969P0

- IGSF8_HUMAN

UniProt

Q969P0 - IGSF8_HUMAN

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Protein

Immunoglobulin superfamily member 8

Gene

IGSF8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.5 Publications

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. cellular component movement Source: UniProtKB
  3. nervous system development Source: UniProtKB
  4. single fertilization Source: UniProtKB
  5. skeletal muscle tissue development Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 8
Short name:
IgSF8
Alternative name(s):
CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
Short name:
EWI-2
Keratinocytes-associated transmembrane protein 4
Short name:
KCT-4
LIR-D1
Prostaglandin regulatory-like protein
Short name:
PGRL
CD_antigen: CD316
Gene namesi
Name:IGSF8
Synonyms:CD81P3, EWI2, KCT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:17813. IGSF8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 579552ExtracellularSequence AnalysisAdd
BLAST
Transmembranei580 – 60021HelicalSequence AnalysisAdd
BLAST
Topological domaini601 – 61313CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. integral component of membrane Source: UniProtKB
  3. membrane Source: UniProtKB
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29767.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 613586Immunoglobulin superfamily member 8PRO_0000226247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 127PROSITE-ProRule annotation
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi186 ↔ 270PROSITE-ProRule annotation
Disulfide bondi326 ↔ 406PROSITE-ProRule annotation
Glycosylationi327 – 3271N-linked (GlcNAc...)1 Publication
Disulfide bondi462 ↔ 544PROSITE-ProRule annotation
Glycosylationi463 – 4631N-linked (GlcNAc...)1 Publication
Lipidationi604 – 6041S-palmitoyl cysteineBy similarity
Lipidationi605 – 6051S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ969P0.
PaxDbiQ969P0.
PRIDEiQ969P0.

2D gel databases

UCD-2DPAGEQ969P0.

PTM databases

PhosphoSiteiQ969P0.

Expressioni

Tissue specificityi

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.3 Publications

Gene expression databases

BgeeiQ969P0.
CleanExiHS_IGSF8.
ExpressionAtlasiQ969P0. baseline and differential.
GenevestigatoriQ969P0.

Organism-specific databases

HPAiHPA011917.

Interactioni

Subunit structurei

Interacts directly with CD82, CD81/tetraspanin-28 and CD9/tetraspanin-29. Also interacts with integrin alpha-3/beta-1 and integrin alpha-4/beta-1.4 Publications

Protein-protein interaction databases

BioGridi125011. 179 interactions.
IntActiQ969P0. 1 interaction.
MINTiMINT-4536413.
STRINGi9606.ENSP00000316664.

Structurei

3D structure databases

ProteinModelPortaliQ969P0.
SMRiQ969P0. Positions 32-130, 176-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 149122Ig-like C2-type 1Add
BLAST
Domaini162 – 286125Ig-like C2-type 2Add
BLAST
Domaini303 – 424122Ig-like C2-type 3Add
BLAST
Domaini431 – 560130Ig-like C2-type 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi274 – 2763EWI motif

Domaini

The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.By similarity
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG28691.
GeneTreeiENSGT00390000010278.
HOGENOMiHOG000113020.
HOVERGENiHBG073047.
InParanoidiQ969P0.
KOiK06730.
OMAiVTCCFMK.
OrthoDBiEOG7H791Z.
PhylomeDBiQ969P0.
TreeFamiTF332702.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q969P0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGALRPTLLP PSLPLLLLLM LGMGCWAREV LVPEGPLYRV AGTAVSISCN
60 70 80 90 100
VTGYEGPAQQ NFEWFLYRPE APDTALGIVS TKDTQFSYAV FKSRVVAGEV
110 120 130 140 150
QVQRLQGDAV VLKIARLQAQ DAGIYECHTP STDTRYLGSY SGKVELRVLP
160 170 180 190 200
DVLQVSAAPP GPRGRQAPTS PPRMTVHEGQ ELALGCLART STQKHTHLAV
210 220 230 240 250
SFGRSVPEAP VGRSTLQEVV GIRSDLAVEA GAPYAERLAA GELRLGKEGT
260 270 280 290 300
DRYRMVVGGA QAGDAGTYHC TAAEWIQDPD GSWAQIAEKR AVLAHVDVQT
310 320 330 340 350
LSSQLAVTVG PGERRIGPGE PLELLCNVSG ALPPAGRHAA YSVGWEMAPA
360 370 380 390 400
GAPGPGRLVA QLDTEGVGSL GPGYEGRHIA MEKVASRTYR LRLEAARPGD
410 420 430 440 450
AGTYRCLAKA YVRGSGTRLR EAASARSRPL PVHVREEGVV LEAVAWLAGG
460 470 480 490 500
TVYRGETASL LCNISVRGGP PGLRLAASWW VERPEDGELS SVPAQLVGGV
510 520 530 540 550
GQDGVAELGV RPGGGPVSVE LVGPRSHRLR LHSLGPEDEG VYHCAPSAWV
560 570 580 590 600
QHADYSWYQA GSARSGPVTV YPYMHALDTL FVPLLVGTGV ALVTGATVLG
610
TITCCFMKRL RKR
Length:613
Mass (Da):65,034
Last modified:December 1, 2001 - v1
Checksum:iA7255D54D42EFB88
GO
Isoform 2 (identifier: Q969P0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-315: ELRLGKEGTD...AVTVGPGERR → MRGRSWHWAA...GSFVWARKGP

Note: No experimental confirmation available.

Show »
Length:372
Mass (Da):40,191
Checksum:i4872C8D6CC303638
GO
Isoform 3 (identifier: Q969P0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     475-561: Missing.

Note: No experimental confirmation available.

Show »
Length:526
Mass (Da):55,808
Checksum:iC2A88D773A4F4802
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3031S → R in AAM94901. 1 PublicationCurated
Sequence conflicti306 – 3061A → P in AAM94901. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 241241Missing in isoform 2. 1 PublicationVSP_017429Add
BLAST
Alternative sequencei242 – 31574ELRLG…PGERR → MRGRSWHWAAWRGQAHRSTH TWQCPLGDLCPRHQLGGQLC RKWWESGQTWPWRLELPMLS DWLQGSFVWARKGP in isoform 2. 1 PublicationVSP_017430Add
BLAST
Alternative sequencei475 – 56187Missing in isoform 3. 1 PublicationVSP_017431Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407274 mRNA. Translation: AAL01052.1.
AY044845 mRNA. Translation: AAK92220.1.
AY157579 mRNA. Translation: AAO13163.1.
AF311906 mRNA. Translation: AAM94901.1.
AK055843 mRNA. Translation: BAB71027.1.
BC004108 mRNA. Translation: AAH04108.2.
BC053881 mRNA. Translation: AAH53881.1.
CCDSiCCDS1195.1. [Q969P0-1]
RefSeqiNP_001193594.1. NM_001206665.2. [Q969P0-1]
NP_443100.1. NM_052868.4. [Q969P0-1]
XP_005245671.1. XM_005245614.1. [Q969P0-1]
UniGeneiHs.332012.

Genome annotation databases

EnsembliENST00000314485; ENSP00000316664; ENSG00000162729. [Q969P0-1]
ENST00000368086; ENSP00000357065; ENSG00000162729. [Q969P0-1]
ENST00000614243; ENSP00000477565; ENSG00000162729. [Q969P0-1]
GeneIDi93185.
KEGGihsa:93185.
UCSCiuc001fuz.3. human. [Q969P0-1]

Polymorphism databases

DMDMi74762642.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407274 mRNA. Translation: AAL01052.1 .
AY044845 mRNA. Translation: AAK92220.1 .
AY157579 mRNA. Translation: AAO13163.1 .
AF311906 mRNA. Translation: AAM94901.1 .
AK055843 mRNA. Translation: BAB71027.1 .
BC004108 mRNA. Translation: AAH04108.2 .
BC053881 mRNA. Translation: AAH53881.1 .
CCDSi CCDS1195.1. [Q969P0-1 ]
RefSeqi NP_001193594.1. NM_001206665.2. [Q969P0-1 ]
NP_443100.1. NM_052868.4. [Q969P0-1 ]
XP_005245671.1. XM_005245614.1. [Q969P0-1 ]
UniGenei Hs.332012.

3D structure databases

ProteinModelPortali Q969P0.
SMRi Q969P0. Positions 32-130, 176-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125011. 179 interactions.
IntActi Q969P0. 1 interaction.
MINTi MINT-4536413.
STRINGi 9606.ENSP00000316664.

PTM databases

PhosphoSitei Q969P0.

Polymorphism databases

DMDMi 74762642.

2D gel databases

UCD-2DPAGE Q969P0.

Proteomic databases

MaxQBi Q969P0.
PaxDbi Q969P0.
PRIDEi Q969P0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000314485 ; ENSP00000316664 ; ENSG00000162729 . [Q969P0-1 ]
ENST00000368086 ; ENSP00000357065 ; ENSG00000162729 . [Q969P0-1 ]
ENST00000614243 ; ENSP00000477565 ; ENSG00000162729 . [Q969P0-1 ]
GeneIDi 93185.
KEGGi hsa:93185.
UCSCi uc001fuz.3. human. [Q969P0-1 ]

Organism-specific databases

CTDi 93185.
GeneCardsi GC01M160061.
HGNCi HGNC:17813. IGSF8.
HPAi HPA011917.
MIMi 606644. gene.
neXtProti NX_Q969P0.
PharmGKBi PA29767.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG28691.
GeneTreei ENSGT00390000010278.
HOGENOMi HOG000113020.
HOVERGENi HBG073047.
InParanoidi Q969P0.
KOi K06730.
OMAi VTCCFMK.
OrthoDBi EOG7H791Z.
PhylomeDBi Q969P0.
TreeFami TF332702.

Miscellaneous databases

ChiTaRSi IGSF8. human.
GeneWikii IGSF8.
GenomeRNAii 93185.
NextBioi 78023.
PROi Q969P0.
SOURCEi Search...

Gene expression databases

Bgeei Q969P0.
CleanExi HS_IGSF8.
ExpressionAtlasi Q969P0. baseline and differential.
Genevestigatori Q969P0.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 4 hits.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
    Stipp C.S., Kolesnikova T.V., Hemler M.E.
    J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CD81 AND CD9.
  2. "EWI-2 is a new component of the tetraspanin web in hepatocytes and lymphoid cells."
    Charrin S., Le Naour F., Labas V., Billard M., Le Caer J.-P., Emile J.-F., Petit M.-A., Boucheix C., Rubinstein E.
    Biochem. J. 373:409-421(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  3. "Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes."
    Bonkobara M., Das A., Takao J., Cruz P.D. Jr., Ariizumi K.
    Br. J. Dermatol. 148:654-664(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  4. "Identification of novel membrane proteins."
    Zhang W., Li N., Wan T., Cao X.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Kidney.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  7. "Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini."
    Xu G., Shin S.B., Jaffrey S.R.
    Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 28-40.
    Tissue: Leukemic T-cell.
  8. "EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
    Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
    Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CD82, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  9. "EWI-2 regulates alpha3beta1 integrin-dependent cell functions on laminin-5."
    Stipp C.S., Kolesnikova T.V., Hemler M.E.
    J. Cell Biol. 163:1167-1177(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH INTEGRIN ALPHA-3 BETA-1.
  10. "EWI-2 modulates lymphocyte integrin alpha4beta1 functions."
    Kolesnikova T.V., Stipp C.S., Rao R.M., Lane W.S., Luscinskas F.W., Hemler M.E.
    Blood 103:3013-3019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH INTEGRIN ALPHA-4 BETA-1.
  11. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-327 AND ASN-463.
    Tissue: Liver.

Entry informationi

Entry nameiIGSF8_HUMAN
AccessioniPrimary (citable) accession number: Q969P0
Secondary accession number(s): Q8NG09, Q96DP4, Q9BTG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3