Reviewed, UniProtKB/Swiss-Prot Q969M3 (YIPF5_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein YIPF5
Alternative name(s):
    YIP1 family member 5
    YPT-interacting protein 1 A
    Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5
    Smooth muscle cell-associated protein 5
      Short name=SMAP-5

Gene names

Name:	YIPF5
Synonyms:	FINGER5, YIP1A
ORF Names:	PP12723, SB140, UNQ3123/PRO10275

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in transport between endoplasmic reticulum and Golgi. Ref.1 Ref.11
Subunit structure	Interacts with the COPII coat components Sec23 (SEC23A and/or SEC23B) and Sec24 (SEC24A and/or SEC24B). Interacts with YIF1A. May interact with RAB1A. Ref.1 Ref.11 Ref.12
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus › Golgi stack membrane; Multi-pass membrane protein. Cytoplasmic vesicle › COPII-coated vesicle. Note: Enriched at the endoplasmic reticulum exit sites. Incorporated into COPII coated vesicles. Ref.1 Ref.12
Tissue specificity	Ubiquitously expressed. Highly expressed in coronary smooth muscles. Ref.2
Induction	By TGFB1. Ref.2
Sequence similarities	Belongs to the YIP1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ER to Golgi transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q969M3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q969M3-2)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Isoform 3 (identifier: Q969M3-3)

Also known as: I;

The sequence of this isoform differs from the canonical sequence as follows:
     242-257: YPCALLYGVFALISVF → LQPNITYGSNYFLFCCLPYPQQHF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Protein YIPF5		PRO_0000234328
Regions
	Topological domain	1 – 124	124	Cytoplasmic Potential
	Transmembrane	125 – 145	21	Potential
	Topological domain	146	1	Lumenal Potential
	Transmembrane	147 – 167	21	Potential
	Topological domain	168 – 173	6	Cytoplasmic Potential
	Transmembrane	174 – 194	21	Potential
	Topological domain	195 – 196	2	Lumenal Potential
	Transmembrane	197 – 217	21	Potential
	Topological domain	218 – 236	19	Cytoplasmic Potential
	Transmembrane	237 – 257	21	Potential
	Region	75 – 106	32	Interaction with Sec23
Amino acid modifications
	Modified residue	39	1	Phosphotyrosine Ref.13
	Modified residue	44	1	Phosphotyrosine Ref.13
Natural variations
	Alternative sequence	1 – 54	54	Missing in isoform 2.		VSP_018253
	Alternative sequence	242 – 257	16	YPCAL…LISVF → LQPNITYGSNYFLFCCLPYP QQHF in isoform 3.		VSP_018254
Experimental info
	Sequence conflict	136 – 138	3	AFG → DLA in AAK67644. Ref.3
	Sequence conflict	155	1	I → S in AAG48521. Ref.1
	Sequence conflict	187	1	C → W in AAK67644. Ref.3
	Sequence conflict	192	1	I → Y in AAG48521. Ref.1
	Sequence conflict	244	1	C → R in BAD97235. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: B30EACB0E514DC67	FASTA	257	27,989
	10 20 30 40 50 60 MSGFENLNTD FYQTSYSIDD QSQQSYDYGG SGGPYSKQYA GYDYSQQGRF VPPDMMQPQQ 70 80 90 100 110 120 PYTGQIYQPT QAYTPASPQP FYGNNFEDEP PLLEELGINF DHIWQKTLTV LHPLKVADGS 130 140 150 160 170 180 IMNETDLAGP MVFCLAFGAT LLLAGKIQFG YVYGISAIGC LGMFCLLNLM SMTGVSFGCV 190 200 210 220 230 240 ASVLGYCLLP MILLSSFAVI FSLQGMVGII LTAGIIGWCS FSASKIFISA LAMEGQQLLV 250 AYPCALLYGV FALISVF
	Isoform 2 (D). Checksum: C29C76D8D3D7FF6F	FASTA	203	21,896
	10 20 30 40 50 60 MMQPQQPYTG QIYQPTQAYT PASPQPFYGN NFEDEPPLLE ELGINFDHIW QKTLTVLHPL 70 80 90 100 110 120 KVADGSIMNE TDLAGPMVFC LAFGATLLLA GKIQFGYVYG ISAIGCLGMF CLLNLMSMTG 130 140 150 160 170 180 VSFGCVASVL GYCLLPMILL SSFAVIFSLQ GMVGIILTAG IIGWCSFSAS KIFISALAME 190 200 GQQLLVAYPC ALLYGVFALI SVF
	Isoform 3 (I). Checksum: DBAD311C26DE5A05	FASTA	265	29,107
	10 20 30 40 50 60 MSGFENLNTD FYQTSYSIDD QSQQSYDYGG SGGPYSKQYA GYDYSQQGRF VPPDMMQPQQ 70 80 90 100 110 120 PYTGQIYQPT QAYTPASPQP FYGNNFEDEP PLLEELGINF DHIWQKTLTV LHPLKVADGS 130 140 150 160 170 180 IMNETDLAGP MVFCLAFGAT LLLAGKIQFG YVYGISAIGC LGMFCLLNLM SMTGVSFGCV 190 200 210 220 230 240 ASVLGYCLLP MILLSSFAVI FSLQGMVGII LTAGIIGWCS FSASKIFISA LAMEGQQLLV 250 260 ALQPNITYGS NYFLFCCLPY PQQHF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A membrane protein enriched in endoplasmic reticulum exit sites interacts with COPII." Tang B.L., Ong Y.S., Huang B., Wei S., Wong E.T., Qi R., Horstmann H., Hong W. J. Biol. Chem. 276:40008-40017(2001) [PubMed: 11489904] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH SEC23 AND SEC24, SUBCELLULAR LOCATION.
[2]	"Cloning, cellular localization, genomic organization, and tissue-specific expression of the TGFbeta1-inducible SMAP-5 gene." Stolle K., Schnoor M., Fuellen G., Spitzer M., Engel T., Spener F., Cullen P., Lorkowski S. Gene 351:119-130(2005) [PubMed: 15922870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY, INDUCTION. Tissue: Coronary artery.
[3]	Zhang W., Li N., Zhang M., Wan T., Cao X. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Neuroblastoma.
[6]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney epithelium.
[8]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Adipose tissue.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix, Eye and Uterus.
[10]	"Molecular cloning and characterization of the human smooth muscle cell associated protein-5 (SMAP-5)." Nishimoto S., Toyoda H., Tawara J., Aoki T., Komurasaki T. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-257 (ISOFORMS 1/2). Tissue: Heart.
[11]	"Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor." Chen C.Z., Calero M., DeRegis C.J., Heidtman M., Barlowe C., Collins R.N. Genetics 168:1827-1841(2004) [PubMed: 15611160] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAB1A.
[12]	"Human Yip1A specifies the localization of Yif1 to the Golgi apparatus." Jin C., Zhang Y., Zhu H., Ahmed K., Fu C., Yao X. Biochem. Biophys. Res. Commun. 334:16-22(2005) [PubMed: 15990086] [Abstract] Cited for: INTERACTION WITH YIF1A, SUBCELLULAR LOCATION.
[13]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-39 AND TYR-44, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF140225 mRNA. Translation: AAG48521.1. AY640925 mRNA. Translation: AAV51256.1. AY640926 mRNA. Translation: AAV51257.1. AY640927 mRNA. Translation: AAV51258.1. AY640928 mRNA. Translation: AAV51259.1. AY640929 mRNA. No translation available. AY640934 mRNA. Translation: AAV51260.1. AY037152 mRNA. Translation: AAK67644.1. AY358863 mRNA. Translation: AAQ89222.1. AK054576 mRNA. Translation: BAB70763.1. AF318329 mRNA. Translation: AAL55836.1. AK223515 mRNA. Translation: BAD97235.1. CR749463 mRNA. Translation: CAH18295.1. BC007829 mRNA. Translation: AAH07829.1. BC014253 mRNA. Translation: AAH14253.1. BC024737 mRNA. Translation: AAH24737.1. AB014733 mRNA. Translation: BAB20270.1.
IPI	IPI00334657. IPI00746973. IPI00748093.
RefSeq	NP_001020118.1. NP_110426.4.
UniGene	Hs.372050
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q969M3. 1 interaction.
PTM databases
PhosphoSite	Q969M3.
Proteomic databases
PRIDE	Q969M3.
Genome annotation databases
Ensembl	ENSG00000145817. Homo sapiens. [Contig view]
GeneID	81555.
KEGG	hsa:81555.
Organism-specific databases
GeneCards	GC05M143520.
H-InvDB	HIX0004184. HIX0005274.
HGNC	HGNC:24877. YIPF5.
MIM	611483. gene.
PharmGKB	PA142670548.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q969M3.
OMA	Q969M3. ISALAMD.
Gene expression databases
ArrayExpress	Q969M3.
Bgee	Q969M3.
CleanEx	HS_YIPF5.
GermOnline	ENSG00000145817. Homo sapiens.
Family and domain databases
InterPro	IPR006977. Yip1. [Graphical view]
Pfam	PF04893. Yip1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	71810.
SOURCE	Search...

Entry information

Entry name

YIPF5_HUMAN

Accession

Primary (citable) accession number: Q969M3
Secondary accession number(s): Q4VSN6 , Q53EX4, Q8NHE5, Q9H338, Q9H3U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)